Electric field staining buffer reagent for promoting in vitro biological tissue staining effect and its application

Abstract

The present invention discloses an electric field staining buffer reagent and its application for promoting the staining effect of isolated biological tissues. The electric field staining buffer reagent is formed by dissolving a pH buffer, a nonionic surfactant, and a protective agent in distilled water. During the staining process, the pH buffer is used to maintain the pH stability of the solution under neutral or alkaline conditions, which is conducive to the penetration of antibodies into deeper tissues. The nonionic surfactant is used to reduce the nonspecific binding between antibodies and other proteins and promote the specific binding of antibodies and proteins. The protective agent helps maintain the morphology and stability of cells and prevents protein degradation. The electric field staining buffer reagent is used to stain isolated biological tissues. The electric field staining buffer of the present invention can greatly shorten the labeling time of human and animal tissues, improve the depth and uniformity of tissue labeling, and does not cause any significant damage to the shape and microstructure of biological tissues.

Images