A traditional Chinese medicine composition for treating endometritis of dairy cows

Abstract

The present application relates to the technical field of endometritis treatment, and discloses a traditional Chinese medicine composition for treating endometritis of dairy cows, which comprises traditional Chinese medicine main materials and auxiliary agents, and the traditional Chinese medicine main materials comprise the following components in parts by weight: 10-15 parts of chuanxiong, 8-12 parts of safflower, 8-12 parts of angelica, 3-5 parts of peach kernel, 6-8 parts of coptis, 6-8 parts of forsythia, 2-4 parts of prepared rehmannia and 1-2 parts of five spirit resins. The traditional Chinese medicine composition prepared by the present application can promote the repair of postpartum uterine tissue of dairy cows, reduce the generation of bacteria in the postpartum uterus of dairy cows, reduce the occurrence of endometritis and the risk of bacterial infection, improve the immune anti-inflammatory ability of the body, prevent and treat metritis, promote the rapid estrus and breeding of dairy cows, and improve the pregnancy rate. The traditional Chinese medicine composition has remarkable curative effect on treating endometritis of dairy cows, has little drug residue, and has small toxic and side effects.

Description

Technical Field

[0001] This invention relates to the field of endometritis treatment technology, specifically a traditional Chinese medicine composition for treating endometritis in dairy cows. Background Technology

[0002] Bovine endometritis is an inflammation of the uterine lining in dairy cows after calving. It can prolong the intercalation period, reduce milk production, increase culling rates, and raise treatment costs. Common causes include birth canal injury, dystocia, abortion, uterine prolapse, vaginal prolapse, vaginitis, cervicitis, lochia retention, retained placenta, and inadequate sterilization during artificial insemination or vaginal examinations, allowing viral infections to invade the uterus. Currently, antibiotics are the primary treatment for bovine endometritis. However, due to the continuous emergence of drug-resistant strains, the efficacy of these drugs has significantly decreased, and drug residues are easily left in livestock products.

[0003] Chinese patent discloses a traditional Chinese medicine infusion solution for treating endometritis in dairy cows and its preparation process (authorization announcement number CN104173630B). This patented technology has a good antibacterial effect against the pathogens causing endometritis in dairy cows, but it is not good at improving the body's immune and anti-inflammatory capabilities after calving; and it cannot effectively promote estrus, mating, and conception in dairy cows. Summary of the Invention

[0004] The purpose of this invention is to provide a traditional Chinese medicine composition for treating endometritis in dairy cows, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] A traditional Chinese medicine composition for treating endometritis in dairy cows includes main Chinese medicinal materials and auxiliary agents. The main Chinese medicinal materials include the following components by weight: 10-15 parts of Ligusticum chuanxiong, 8-12 parts of Carthamus tinctorius, 8-12 parts of Angelica sinensis, 3-5 parts of Prunus persica, 6-8 parts of Coptis chinensis, 6-8 parts of Forsythia suspensa, 2-4 parts of Rehmannia glutinosa, and 1-2 parts of Trogopterus xanthipes.

[0007] As a further embodiment of the present invention, the auxiliary agent comprises the following components by weight: 10-12 parts cyclodextrin, 3-5 parts sodium carboxymethyl starch, 3-5 parts microcrystalline cellulose, 2-3 parts micronized silica gel, 2-3 parts calcium sulfate, 15-20 parts ethanol, 3-5 parts mannitol, 3-5 parts xylitol and 8-10 parts anhydrous lactose.

[0008] As a further embodiment of the present invention, the preparation method includes the following steps:

[0009] S1. Take 10-15 parts of Ligusticum chuanxiong, 8-12 parts of Carthamus tinctorius, 8-12 parts of Angelica sinensis, 3-5 parts of Prunus persica, 6-8 parts of Coptis chinensis, 6-8 parts of Forsythia suspensa, 2-4 parts of Rehmannia glutinosa, and 1-2 parts of Trogopterus xanthipes. Slice each herb and add an appropriate amount of distilled water so that the water level covers the main herbs by 3-5 cm. Soak for 1-2 hours, then bring to a boil over high heat and simmer over low heat for 2-3 hours. Filter to obtain filtrate and residue. Repeat the above method to decoct the residue twice more, and mix the filtrates obtained from the three decoctions. After centrifugation, take the supernatant to obtain the herbal extract.

[0010] S2. The extract of traditional Chinese medicine is concentrated by vacuum evaporation, dried at 55-60℃, pulverized, and passed through an 80-100 mesh sieve to obtain extract powder.

[0011] S3. Add the extract powder to the reaction vessel along with 10-12 parts cyclodextrin, 3-5 parts sodium carboxymethyl starch, 3-5 parts microcrystalline cellulose, 2-3 parts micronized silica gel, 2-3 parts calcium sulfate, 15-20 parts ethanol, 3-5 parts mannitol, 3-5 parts xylitol, and 8-10 parts anhydrous lactose. Stir thoroughly to obtain the soft material of traditional Chinese medicine; then add it to a granulator to obtain the granules of traditional Chinese medicine.

[0012] S5. After the Chinese medicine granules are dried with hot air, they are sized to make the granules uniform, packaged, and then sterilized under high pressure. When giving them to dairy cows, they can be dissolved in warm water and administered by gavage.

[0013] As a further embodiment of the present invention: in step S1, the centrifugation speed is 3000-4000 r / min and the time is 5-8 min.

[0014] As a further embodiment of the present invention: in step S2, the vacuum degree of vacuum evaporation concentration is 70-95 kPa and the temperature is 50-60°C, and the density of the Chinese herbal extract after vacuum evaporation concentration is 1.15-1.25 g / ml.

[0015] As a further aspect of the present invention: in step S5, the temperature of hot air drying is 50-60°C and the time is 2-3 hours.

[0016] As a further aspect of the present invention: in step S5, the pressure of autoclaving is 103-135 kPa and the time is 15-30 min.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0018] The traditional Chinese medicine composition prepared by this invention can promote the repair of uterine tissue in dairy cows after calving, reduce the production of bacteria in the uterus after calving, reduce the occurrence of endometritis and the risk of bacterial infection; improve the body's immune anti-inflammatory ability and prevent endometritis; at the same time, it can also promote rapid estrus and mating in dairy cows and improve the conception rate; the traditional Chinese medicine composition has significant efficacy in treating endometritis in dairy cows, with low drug residue and minimal toxic side effects. Detailed Implementation

[0019] Example 1

[0020] In this embodiment of the invention, a traditional Chinese medicine composition for treating endometritis in dairy cows includes main medicinal materials and auxiliary agents. The main medicinal materials, by weight, include the following components: 10 parts of Ligusticum chuanxiong, 8 parts of Carthamus tinctorius, 8 parts of Angelica sinensis, 3 parts of Prunus persica, 6 parts of Coptis chinensis, 6 parts of Forsythia suspensa, 2 parts of Rehmannia glutinosa, and 1 part of Trogopterus xanthipes. The auxiliary agents, by weight, include the following components: 10 parts of cyclodextrin, 3 parts of sodium carboxymethyl starch, 3 parts of microcrystalline cellulose, 2 parts of microcrystalline silica gel, 2 parts of calcium sulfate, 15 parts of ethanol, 3 parts of mannitol, 3 parts of xylitol, and 8 parts of anhydrous lactose.

[0021] The preparation method of this traditional Chinese medicine composition includes the following steps:

[0022] S1. Take 10 parts of Ligusticum chuanxiong, 8 parts of Carthamus tinctorius, 8 parts of Angelica sinensis, 3 parts of Prunus persica, 6 parts of Coptis chinensis, 6 parts of Forsythia suspensa, 2 parts of Rehmannia glutinosa, and 1 part of Trogopterus xanthipes. Slice each ingredient separately and add an appropriate amount of distilled water so that the water level covers the main medicinal materials by 3 cm. Soak for 1 hour, then bring to a boil over high heat and simmer over low heat for 3 hours. After filtration, obtain the filtrate and the residue. Then, simmer the residue twice using the above method and mix the filtrates obtained from the three simmerings. Centrifuge at 3000 r / min for 8 minutes and take the supernatant to obtain the medicinal extract.

[0023] S2. The herbal extract was concentrated by vacuum evaporation at 70 kPa and 50°C to a density of 1.15 g / ml; then dried at 55°C, pulverized, and passed through an 80-mesh sieve to obtain extract powder.

[0024] S3. Add the extract powder to the reaction vessel, along with 10 parts cyclodextrin, 3 parts sodium carboxymethyl starch, 3 parts microcrystalline cellulose, 2 parts micronized silica gel, 2 parts calcium sulfate, 15 parts ethanol, 3 parts mannitol, 3 parts xylitol, and 8 parts anhydrous lactose. Stir and mix thoroughly to obtain the soft material of traditional Chinese medicine; then add it to the granulator to obtain the granules of traditional Chinese medicine.

[0025] S5. Dry the Chinese medicine granules with hot air at 50℃ for 3 hours, granulate them to make them uniform, pack them into bags, and then sterilize them under high pressure at 103kPa for 30 minutes.

[0026] Example 2

[0027] In this embodiment of the invention, a traditional Chinese medicine composition for treating endometritis in dairy cows includes main medicinal materials and adjuvants. The main medicinal materials, by weight, include the following components: 12 parts of Ligusticum chuanxiong, 10 parts of Carthamus tinctorius, 10 parts of Angelica sinensis, 4 parts of Prunus persica, 7 parts of Coptis chinensis, 7 parts of Forsythia suspensa, 3 parts of Rehmannia glutinosa, and 1.5 parts of Trogopterus xanthipes. The adjuvants, by weight, include the following components: 11 parts of cyclodextrin, 4 parts of sodium carboxymethyl starch, 4 parts of microcrystalline cellulose, 2.5 parts of microcrystalline silica gel, 2.5 parts of calcium sulfate, 18 parts of ethanol, 4 parts of mannitol, 4 parts of xylitol, and 9 parts of anhydrous lactose.

[0028] The preparation method of this traditional Chinese medicine composition includes the following steps:

[0029] S1. Take 12 parts of Ligusticum chuanxiong, 10 parts of Carthamus tinctorius, 10 parts of Angelica sinensis, 4 parts of Prunus persica, 7 parts of Coptis chinensis, 7 parts of Forsythia suspensa, 3 parts of Rehmannia glutinosa, and 1.5 parts of Trogopterus xanthipes. Slice each herb and add an appropriate amount of distilled water so that the water level covers the main herb by 4 cm. Soak for 1.5 hours, bring to a boil over high heat, and then simmer over low heat for 2.5 hours. Filter to obtain filtrate and residue. Then, simmer the residue twice using the above method and mix the filtrates obtained from the three simmerings. Centrifuge at 3500 r / min for 6 minutes and take the supernatant to obtain the herbal extract.

[0030] S2. The herbal extract was concentrated by vacuum evaporation at 85 kPa and 55°C to a density of 1.20 g / ml; then dried at 58°C, pulverized, and passed through a 90-mesh sieve to obtain extract powder.

[0031] S3. Add the extract powder to the reaction vessel, along with 11 parts cyclodextrin, 4 parts sodium carboxymethyl starch, 4 parts microcrystalline cellulose, 2.5 parts micronized silica gel, 2.5 parts calcium sulfate, 18 parts ethanol, 4 parts mannitol, 4 parts xylitol, and 9 parts anhydrous lactose. Stir and mix thoroughly to obtain the soft material of traditional Chinese medicine; then add it to the granulator to obtain the granules of traditional Chinese medicine.

[0032] S5. Dry the Chinese medicine granules with hot air at 58℃ for 2.5 hours, granulate them to make them uniform, pack them into bags, and then sterilize them under high pressure at 120kPa for 20 minutes.

[0033] Example 3

[0034] In this embodiment of the invention, a traditional Chinese medicine composition for treating endometritis in dairy cows includes main Chinese medicinal materials and auxiliary agents. The main Chinese medicinal materials include the following components by weight: 15 parts of Ligusticum chuanxiong, 12 parts of Carthamus tinctorius, 12 parts of Angelica sinensis, 5 parts of Prunus persica, 8 parts of Coptis chinensis, 8 parts of Forsythia suspensa, 4 parts of Rehmannia glutinosa, and 2 parts of Trogopterus xanthipes.

[0035] The excipients, by weight, include the following components: 12 parts cyclodextrin, 5 parts sodium carboxymethyl starch, 5 parts microcrystalline cellulose, 3 parts micronized silica gel, 3 parts calcium sulfate, 20 parts ethanol, 5 parts mannitol, 5 parts xylitol and 10 parts anhydrous lactose.

[0036] The preparation method of this traditional Chinese medicine composition includes the following steps:

[0037] S1. Take 15 parts of Ligusticum chuanxiong, 12 parts of Carthamus tinctorius, 12 parts of Angelica sinensis, 5 parts of Prunus persica, 8 parts of Coptis chinensis, 8 parts of Forsythia suspensa, 4 parts of Rehmannia glutinosa, and 2 parts of Trogopterus xanthipes. Slice each herb and add an appropriate amount of distilled water so that the water level covers the main herb by 5 cm. Soak for 2 hours, then bring to a boil over high heat and simmer over low heat for 2 hours. Filter to obtain filtrate and residue. Repeat the above method to decoct the residue twice, and mix the filtrates obtained from the three decoctions. Centrifuge at 4000 r / min for 5 minutes and take the supernatant to obtain the herbal extract.

[0038] S2. The herbal extract was concentrated by vacuum evaporation at 50°C and 95 kPa to a density of 1.25 g / ml; then dried at 60°C, pulverized, and passed through a 100-mesh sieve to obtain extract powder.

[0039] S3. Add the extract powder to the reaction vessel, along with 12 parts cyclodextrin, 5 parts sodium carboxymethyl starch, 5 parts microcrystalline cellulose, 3 parts micronized silica gel, 3 parts calcium sulfate, 20 parts ethanol, 5 parts mannitol, 5 parts xylitol, and 10 parts anhydrous lactose. Stir and mix thoroughly to obtain the soft material of traditional Chinese medicine; then add it to the granulator to obtain the granules of traditional Chinese medicine.

[0040] S5. Dry the Chinese medicine granules with hot air at 60℃ for 2 hours, granulate them to make them uniform, pack them into bags, and then sterilize them under high pressure at 135kPa for 15 minutes.

[0041] To better illustrate the technical effects of the present invention, the following experiments are conducted:

[0042] I. Diagnosis of endometritis in dairy cows: In large-scale dairy farms, dairy cows with no significant differences in age, parity, weight, and milk yield were selected. Based on clinical symptoms, number of days without pregnancy, vaginal discharge, and uterine condition upon rectal examination, a comprehensive diagnosis was made, and 50 cases of endometritis in dairy cows were diagnosed; among them, 12 cases were acute endometritis and 38 cases were chronic endometritis.

[0043] II. Grouping and Administration: The 50 dairy cows were randomly divided into 5 groups of 10 each, namely, a traditional Chinese medicine (TCM) treatment group 1, a TCM treatment group 2, a TCM treatment group 3, a chemical drug treatment group, and a pathological control group. In the TCM treatment groups 1, 2, and 3, each cow was administered the TCM composition prepared in Examples 1-3 of this invention by gavage. The composition was dissolved in warm water, and 500g was administered to each cow once daily for 7 days as one course of treatment, with two courses of treatment. The chemical drug treatment group used a commonly used treatment method in the farm, namely, dissolving 2g of oxytetracycline powder, 2g of tetracycline powder, 1g of chlortetracycline, 1 million units of penicillin, and 0.5g of streptomycin in 200ml of distilled water and injecting it into the uterus of each cow once. The pathological group was administered 500g of physiological saline once daily for 14 days.

[0044] (1) Uterine morphology analysis: At 7, 14, 21 and 28 days postpartum, the uterine morphology indicators of dairy cows in the traditional Chinese medicine treatment group 1, traditional Chinese medicine treatment group 2, traditional Chinese medicine treatment group 3, chemical drug treatment group and pathological control group were recorded in Table 1 below. The cervical longitudinal diameter and uterine horn wall thickness were measured by rectal examination.

[0045] Table 1. Analysis of Uterine Morphology

[0046]

[0047] Table 1 shows that in dairy cows with endometritis, the cervical longitudinal diameter and uterine horn wall thickness all showed a decreasing trend within 7-28 days postpartum in the traditional Chinese medicine treatment groups (Group 1, Group 2, Group 3, Group 4, and the pathological control group). However, the rate of decrease in cervical longitudinal diameter and uterine horn wall thickness was faster in the traditional Chinese medicine treatment groups (Group 1, Group 2, Group 3, and the Group 4). Among these, the rate of decrease in cervical longitudinal diameter and uterine horn wall thickness in the traditional Chinese medicine treatment groups (Group 1, Group 2, and Group 3) was similar to that in the Group 4. Therefore, it can be concluded that the traditional Chinese medicine composition prepared in this invention can promote the repair of postpartum uterine tissue in dairy cows and reduce the occurrence of endometritis.

[0048] (2) Analysis of uterine secretions: After cleaning the vulva of dairy cows in the Chinese medicine treatment group 1, Chinese medicine treatment group 2, Chinese medicine treatment group 3, chemical drug treatment group and pathological control group with disinfectant, the vulva was opened with a vulvar dilator, and a sterile cotton swab was inserted into the cervix and gently rotated to collect uterine secretions. The color, odor and viscosity of the secretions were observed and recorded in Table 2 below.

[0049] Table 2 Analysis of Uterine Secretions

[0050]

[0051]

[0052] Table 2 shows that the secretions from cows in the traditional Chinese medicine treatment groups 1, 2, and 3, and the chemical drug treatment group were all white, transparent, thin, and had a bloody smell but no foul odor. In contrast, the secretions from cows in the pathological control group were yellowish-white, cloudy, flocculent, viscous, and had an unpleasant and putrid smell. Therefore, it can be concluded that the traditional Chinese medicine composition prepared in this invention can reduce the growth of bacteria in the uterus of cows after calving and reduce the risk of bacterial infection in cows after calving.

[0053] (3) Analysis of estrus and conception: The average time to first estrus (unit: d), the time to first mating (unit: d), and the conception rate (unit: 100%) within 2 estrous cycles were tracked and recorded in dairy cows in the Chinese medicine treatment group 1, Chinese medicine treatment group 2, Chinese medicine treatment group 3, chemical drug treatment group and pathological control group after medication; and recorded in Table 3 below.

[0054] Table 3. Analysis of Estrus and Conception

[0055]

[0056] Table 3 shows that the first estrus and first mating times of dairy cows in the traditional Chinese medicine treatment groups 1, 2, and 3 and the chemical drug treatment group were similar and significantly lower than those in the pathological control group. Furthermore, the conception rate of dairy cows in the traditional Chinese medicine treatment groups 1, 2, and 3 and the chemical drug treatment group was significantly higher than that in the pathological control group. Therefore, it can be concluded that the traditional Chinese medicine composition prepared in this invention can promote rapid estrus and mating in dairy cows, while also increasing the conception rate.

[0057] (4) Blood biochemical index analysis: Blood samples were collected from dairy cows in the following groups before treatment, on day 3 during treatment, and on days 1, 7, and 15 after treatment: Traditional Chinese medicine treatment group 1, Traditional Chinese medicine treatment group 2, Traditional Chinese medicine treatment group 3, chemical drug treatment group, and pathological control group. Blood biochemical indexes were tested. The biochemical index analysis included interleukin-1 (IL-1), interleukin-2 (IL-2), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ). The unit was pg / mL. The results were recorded in Table 4 below.

[0058] Table 3. Blood Biochemical Indicators Analysis Table

[0059]

[0060]

[0061] Table 4 shows that before treatment, there were no significant differences in interleukin-1, interleukin-2, interleukin-8, tumor necrosis factor-α, and interferon-γ levels in dairy cows across the traditional Chinese medicine (TCM) treatment groups 1, 2, and 3, and the chemical drug treatment group. However, after treatment, over time, the levels of interleukin-1, interleukin-2, interleukin-8, tumor necrosis factor-α, and interferon-γ in all three groups continuously decreased. In contrast, in the pathological control group, the levels of interleukin-1, interleukin-2, interleukin-8, tumor necrosis factor-α, and interferon-γ in dairy cows continuously increased until day 15 after treatment, at which point they began to stabilize or slightly decrease. Therefore, it can be concluded that the TCM composition prepared in this invention can significantly reduce the levels of interleukin-1, interleukin-2, interleukin-8, tumor necrosis factor-α, and interferon-γ in the serum of dairy cows with postpartum metritis, thereby improving the body's immune anti-inflammatory capabilities and preventing metritis.

[0062] The above description is merely a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A traditional Chinese medicine composition for treating endometritis of dairy cows, comprising a main material of traditional Chinese medicine and an auxiliary agent, characterized in that, The main Chinese medicinal materials are as follows by weight: 10-15 parts of Ligusticum chuanxiong, 8-12 parts of Carthamus tinctorius, 8-12 parts of Angelica sinensis, 3-5 parts of Prunus persica, 6-8 parts of Coptis chinensis, 6-8 parts of Forsythia suspensa, 2-4 parts of Rehmannia glutinosa, and 1-2 parts of Trogopterus xanthipes; the adjuvants are as follows by weight: 10-12 parts of cyclodextrin, 3-5 parts of sodium carboxymethyl starch, 3-5 parts of microcrystalline cellulose, 2-3 parts of microcrystalline silica gel, 2-3 parts of calcium sulfate, 15-20 parts of ethanol, 3-5 parts of mannitol, 3-5 parts of xylitol, and 8-10 parts of anhydrous lactose; The preparation method of the traditional Chinese medicine composition includes the following steps: S1. Take 10-15 parts of Ligusticum chuanxiong, 8-12 parts of Carthamus tinctorius, 8-12 parts of Angelica sinensis, 3-5 parts of Prunus persica, 6-8 parts of Coptis chinensis, 6-8 parts of Forsythia suspensa, 2-4 parts of Rehmannia glutinosa, and 1-2 parts of Trogopterus xanthipes. Slice each herb and add an appropriate amount of distilled water so that the water level covers the main herbs by 3-5 cm. Soak for 1-2 hours, then bring to a boil over high heat and simmer over low heat for 2-3 hours. Filter to obtain filtrate and residue. Repeat the above method to decoct the residue twice more, and mix the filtrates obtained from the three decoctions. After centrifugation, take the supernatant to obtain the herbal extract. S2. The extract of traditional Chinese medicine is concentrated by vacuum evaporation, dried at 55-60℃, pulverized, and passed through an 80-100 mesh sieve to obtain extract powder. S3. Add the extract powder to the reaction vessel along with 10-12 parts cyclodextrin, 3-5 parts sodium carboxymethyl starch, 3-5 parts microcrystalline cellulose, 2-3 parts micronized silica gel, 2-3 parts calcium sulfate, 15-20 parts ethanol, 3-5 parts mannitol, 3-5 parts xylitol, and 8-10 parts anhydrous lactose. Stir thoroughly to obtain the soft material of traditional Chinese medicine; then add it to a granulator to obtain the granules of traditional Chinese medicine. S5. After the Chinese medicine granules are dried with hot air, they are sized to make the granules uniform, then bagged and sterilized under high pressure. In step S1, the centrifugation speed is 3000-4000 r / min and the time is 5-8 min; In step S2, the vacuum degree of the vacuum evaporation concentration is 70-95 kPa, the temperature is 50-60℃, and the density of the Chinese herbal extract after vacuum evaporation concentration is 1.15-1.25 g / ml. In step S5, the hot air drying temperature is 50-60℃ and the time is 2-3 hours; In step S5, the pressure for autoclaving is 103–135 kPa, and the time is 15–30 min.

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