水稻PD1基因及其在提高植物抗非生物胁迫中的用途

By constructing a PD1 function-deficient vector for rice using CRISPR technology, the germination rate and seedling rate of rice under low temperature were improved, solving the problem of insufficient cold resistance during the germination period of rice, enhancing the stress resistance of rice, and promoting the process of molecular breeding.

CN118652875BActive Publication Date: 2026-07-17NANJING AGRICULTURAL UNIVERSITY

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Filing Date
2024-07-19
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Rice germination and seedling rates are low under low temperature conditions, leading to reduced yield. Current technologies lack effective gene cloning and regulation methods to improve its cold tolerance during germination.

Method used

A recombinant expression vector with loss of PD1 function in rice was constructed using CRISPR technology and transformed into plant tissues to cultivate transgenic plants with improved resistance to abiotic stresses, particularly improving germination rate and seedling rate under low temperature stress.

Benefits of technology

It significantly improved the germination rate and seedling rate of rice under low temperature conditions, enhanced the rice's stress resistance, adapted it to direct seeding technology, and promoted the process of stress-resistant molecular breeding.

✦ Generated by Eureka AI based on patent content.

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Abstract

本发明分子生物学技术领域,公开了水稻PD1基因及其在提高植物抗非生物胁迫能力尤其是耐低温胁迫能力中的应用。本发明采用CRISPR敲除技术将水稻PD1基因在水稻中进行敲除突变,敲除突变体可以显著提高低温下水稻的发芽率和成苗率。本发明证明了水稻PD1基因功能的缺失能够提高水稻对非生物逆境抗性,尤其是耐冷性,可提高水稻在低温胁迫下快速萌发,对于保证直播稻田间出苗具有重要意义,为促进直播稻生产提供重要线索。
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