A traditional Chinese medicine composition for inhibiting oral bacteria, a preparation method thereof and application thereof

Abstract

The application belongs to the field of traditional Chinese medicine preparations, and particularly relates to a traditional Chinese medicine composition for preventing and treating oral diseases, and a preparation method and application thereof. The traditional Chinese medicine composition is composed of insect medicine, a plant in Caprifoliaceae, and a plant in Labiatae. The plant in Caprifoliaceae has the effects of resisting and eliminating inflammation, clearing heat and detoxifying, the plant in Labiatae has the effects of resisting bacteria and eliminating inflammation, and the insect medicine has the effects of activating blood and unblocking collaterals, astringing and healing, and repairing mucosa. The three medicines are combined, and have the effects of regulating ascending and descending, regulating cold and heat, and invigorating the spleen to eliminate dampness, and healing and stopping bleeding. The traditional Chinese medicine composition can effectively prevent and treat common oral diseases such as gingivitis, oral ulcer, periodontal disease, and the like.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine preparations, specifically relating to a traditional Chinese medicine composition for the prevention and treatment of oral diseases, its preparation method, and its application. Background Technology

[0002] Oral diseases are a common problem affecting human health, including gingivitis, periodontitis, oral ulcers, halitosis, and tooth decay. These diseases not only cause pain and discomfort but can also lead to more serious health problems, such as systemic infections and cardiovascular diseases. Therefore, timely and effective prevention and treatment of oral diseases are particularly important.

[0003] Mouthwash, as an auxiliary oral cleaning product, is widely used in daily oral care. Its main functions include: freshening breath, reducing plaque and food debris in the mouth, and preventing gingivitis and other oral diseases. However, most mouthwashes on the market are primarily composed of chemical ingredients, and long-term use may cause side effects such as oral mucosal irritation and bacterial imbalance.

[0004] Traditional Chinese medicine (TCM) herbs have a long history of use and significant clinical efficacy. Many TCM herbs have been proven to have unique advantages in anti-inflammatory, antibacterial, antiviral, and tissue repair-promoting effects. Therefore, developing a TCM composition that can effectively prevent and treat oral diseases while avoiding the side effects of chemical components is of great practical significance for people's oral health.

[0005] Currently, while some oral care products are based on single Chinese herbal ingredients, there is a lack of systematic research on combinations of Chinese herbal medicines, making it difficult to comprehensively address the prevention and treatment needs of various oral diseases. Therefore, there is an urgent need for a scientifically formulated, highly targeted, low-toxicity, and harmless Chinese herbal mouthwash to provide a more effective solution for the prevention and treatment of oral diseases.

[0006] Considering the above background, this paper proposes... Summary of the Invention

[0007] To overcome the limitations of Western medicine, such as its single target and strong toxic side effects, and the irritation of the oral mucosa by traditional mouthwashes and alcohol-based bactericides, and the lack of a systematic approach to the advantages of traditional Chinese medicine and the limitations of Western medicine, this invention aims to provide a traditional Chinese medicine composition for the prevention and treatment of oral diseases, its preparation method, and its application. The traditional Chinese medicine composition of this invention consists of plants from the Caprifoliaceae, Lamiaceae, and insect-derived medicinal materials. The Caprifoliaceae plants possess heat-clearing and detoxifying effects, the Lamiaceae plants have antibacterial, anti-inflammatory, and antioxidant effects, and the insect-derived medicinal materials have blood-activating, astringent, tissue-regenerating, and mucosal-repairing effects.

[0008] The technical solution of the present invention is as follows: a traditional Chinese medicine composition for the prevention and treatment of oral diseases, wherein the raw materials of the traditional Chinese medicine composition are composed of the following components by weight: 10-65 parts of insect medicine, 10-65 parts of heat-clearing and detoxifying medicine, and 10-65 parts of anti-inflammatory and antibacterial medicine.

[0009] Furthermore, the insecticide is selected from one or a mixture of two or more of the following: dung beetles, wasps, giant larvae, horseflies, and water beetles; preferably, giant larvae.

[0010] Furthermore, the heat-clearing and detoxifying medicine is selected from one or more of the following: gardenia, honeysuckle, honeysuckle, elderberry, and honeysuckle.

[0011] Furthermore, the anti-inflammatory and antibacterial drug is selected from one or a mixture of two or more of peppermint, thyme, scutellaria, schizonepeta, patchouli, and salvia miltiorrhiza.

[0012] Furthermore, the Chinese herbal medicine composition comprises the following components by weight: 16-35 parts of insect medicine, 35-60 parts of heat-clearing and detoxifying medicine, and 22-57 parts of anti-inflammatory and antibacterial medicine.

[0013] Furthermore, the Chinese herbal medicine composition comprises the following ingredients by weight: 27 parts of American cockroach, 39 parts of honeysuckle, 21 parts of honeysuckle root, and 24 parts of peppermint.

[0014] Furthermore, the traditional Chinese medicine composition also includes sodium carboxymethyl cellulose, dextrin, starch, glycerol, or citric acid.

[0015] Furthermore, the traditional Chinese medicine composition is selected from capsules, micro-pellets, powders, tablets, granules, solutions, emulsions, or suspensions; preferably, the traditional Chinese medicine composition is selected from solutions.

[0016] This invention also provides a method for preparing the above-mentioned traditional Chinese medicine composition for preventing and treating oral diseases, comprising the following steps:

[0017] 1) Take the whole dried insect medicine, add an appropriate amount of petroleum ether to extract, defatted, and dried at low temperature to obtain the defatted dried body of the insect medicine;

[0018] 2) Thoroughly pulverize the heat-clearing and detoxifying drugs and anti-inflammatory and antibacterial drugs, mix them with the defatted and dried body of the insect medicine, add an appropriate amount of water, heat and extract using a multi-functional extraction tank with the temperature set, and filter with a 400-mesh filter cloth;

[0019] 3) Collect the filtrate, concentrate it into an extract, and add excipients to prepare the final product.

[0020] Preferably, in step 1), the weight of petroleum ether added is 5-20 times that of the dried insecticide, and the extraction is performed 3-5 times for a total extraction time of 12-36 hours; in step 2), the weight of water added is 6-16 times that of the raw material, and the extraction is performed 2-5 times for a total extraction time of 1-4 hours.

[0021] In step 3), the filtrate is concentrated into an extract with a density of 1.20-1.40 g / mL.

[0022] The present invention also provides the application of the traditional Chinese medicine composition in the preparation of drugs for treating and preventing oral diseases, including but not limited to oral ulcers, periodontal disease, or halitosis.

[0023] The present invention also provides the application of the traditional Chinese medicine composition in the preparation of drugs for inhibiting Helicobacter pylori.

[0024] The beneficial effects of this invention: The traditional Chinese medicine composition for the prevention and treatment of oral diseases of this invention is formulated based on traditional Chinese medicine theory, combined with folk medicine experience, and applied using modern scientific theory. It is scientifically formulated by carefully selecting a variety of traditional Chinese medicine ingredients with effective antibacterial and anti-inflammatory effects. Most mouthwashes on the market are mainly chemical agents, which have certain side effects and cannot comprehensively prevent and treat multiple oral diseases. This invention aims to provide a safe, effective, and multi-functional traditional Chinese medicine composition suitable for daily use, which can significantly improve oral hygiene and prevent and treat oral diseases. When the formula contains insect-derived medicine, it can significantly promote mucosal repair; when the formula contains plants of the Honeysuckle family, it has a significant heat-clearing and detoxifying effect; and the addition of plants of the Lamiaceae family has antibacterial and anti-inflammatory effects. When the formula contains insect-derived medicine, plants of the Honeysuckle family, and plants of the Lamiaceae family simultaneously, it can improve oral diseases such as oral ulcers, halitosis, and periodontitis through heat-clearing, detoxifying, antibacterial, and anti-inflammatory effects. The principal ingredient in this formula is an insect-based medicine that invigorates blood circulation, promotes tissue regeneration, and helps heal sores. The secondary ingredient, a plant from the honeysuckle family, clears heat, detoxifies, reduces swelling, and disperses nodules. The plant from the lamiaceae family has significant antibacterial and anti-inflammatory effects, effectively preventing and treating common oral diseases such as gingivitis, oral ulcers, and periodontitis. Detailed Implementation

[0025] The following embodiments are intended to enable those skilled in the art to more fully understand the present invention, but are not intended to limit the invention to the scope of the embodiments described.

[0026] Example 1

[0027] A traditional Chinese medicine composition for the prevention and treatment of oral diseases is prepared from the following raw materials in parts by weight: 31 parts of American cockroach, 41 parts of honeysuckle, and 25 parts of peppermint.

[0028] The traditional Chinese medicine composition is a solution, and the method for preparing the solution is as follows:

[0029] (1) Take the dried insect medicine, add 6 times the amount of petroleum ether, soak and extract at room temperature 4 times, 18 hours each time, take out the insect medicine, and dry at low temperature to obtain the defatted dried body of the insect medicine.

[0030] (2) Take the defatted and dried body of the insect medicine, add 8 times the amount of water, add dried honeysuckle (Lonicera japonica) and peppermint (Mentha haplocalyx) and extract twice with a multi-functional extraction tank for 4 hours each time. Concentrate the filtrate into an extract with a density of 1.34 g / mL, cool, filter and use the extract for later use.

[0031] (3) Take the extract, add an appropriate amount of deionized water, glycerin and citric acid, mix well, and bottle it to obtain the final product.

[0032] Example 2

[0033] A traditional Chinese medicine composition for the prevention and treatment of oral diseases is prepared from the following raw materials in parts by weight: 31 parts of American cockroach (a type of insecticide), 41 parts of honeysuckle, and 25 parts of peppermint.

[0034] The traditional Chinese medicine composition is in the form of capsules, and the preparation method of the capsules is as follows:

[0035] (1) Take the dried insect medicine, add 6 times the amount of petroleum ether, soak and extract at room temperature 4 times, 18 hours each time, take out the insect medicine, and dry at low temperature to obtain the defatted dried body of the insect medicine.

[0036] (2) Take the defatted and dried body of the insect drug, add 8 times the amount of water, extract twice using a multi-functional extraction tank, 4 hours each time, concentrate the filtrate into an extract with a density of 1.34 g / mL, cool, filter, and use the extract for later use.

[0037] (3) The powdered and dried honeysuckle (Lonicera japonica) and peppermint (Mentha haplocalyx) are passed through an 80-mesh sieve. The prescribed amount of extract is added and then processed into a capsule form to obtain the capsule dosage form.

[0038] Example 3

[0039] A traditional Chinese medicine composition for the prevention and treatment of oral diseases is prepared from the following raw materials in parts by weight: 31 parts of American cockroach (a type of insecticide), 41 parts of honeysuckle, and 25 parts of peppermint.

[0040] The traditional Chinese medicine composition is in the form of granules, and the method for preparing the granules is as follows:

[0041] (1) Take dried insect medicine, add 6 times the amount of petroleum ether, soak and extract at room temperature 4 times, 18 hours each time, take out the insect medicine, and dry at low temperature to obtain the defatted dried body of the insect medicine;

[0042] (2) Weigh the prescribed amount of dried insect medicine, add honeysuckle and peppermint, add an appropriate amount of water, use a multi-functional extraction tank to set the temperature and heat to extract, filter with a 400-mesh filter cloth; add an appropriate amount of water to the filter residue, heat to extract, filter, combine the filtrates, add an appropriate amount of ethanol to the water extract, mix and let stand for a period of time, centrifuge, remove the supernatant and retain the precipitate.

[0043] (3) After adding sodium carboxymethyl cellulose, starch and dextrin to the prescribed amount of extract, it is processed into granules to obtain the final product.

[0044] Example 4

[0045] A traditional Chinese medicine composition for the prevention and treatment of oral diseases is prepared from the following raw materials in parts by weight: 31 parts of American cockroach (a type of insecticide), 41 parts of honeysuckle, and 25 parts of peppermint.

[0046] The traditional Chinese medicine composition is in the form of tablets, and the method for preparing the tablets is as follows:

[0047] (1) Take dried insect medicine, add 6 times the amount of petroleum ether, soak and extract at room temperature 4 times, 18 hours each time, take out the insect medicine, and dry at low temperature to obtain the defatted dried body of the insect medicine;

[0048] (2) Weigh the prescribed amount of dried insect medicine, add honeysuckle and peppermint, add an appropriate amount of ethanol to soak, sonicate, collect the extract, repeat the same extraction method three times, mix the three extracts and filter to make a fluid extract.

[0049] (3) Take the above extract, add sodium carboxymethyl cellulose and starch, and process it into tablets to obtain the product.

[0050] Example 5-20

[0051] The specific active pharmaceutical ingredient formulations are shown in Table 1. Extracts of the medicinal materials were prepared according to the methods described in Examples 1-4. After adding necessary pharmaceutical excipients, the corresponding pharmaceutical compositions were prepared using conventional methods.

[0052] Table 1. Active pharmaceutical ingredient ratios and formulation types for Examples 5-20

[0053]

[0054]

[0055] The therapeutic effects of the different prescriptions above show that prescriptions containing American cockroach, honeysuckle, and peppermint are more effective in treating oral diseases. Preferred example 17 prescription: 27 parts American cockroach, 39 parts honeysuckle, 21 parts honeysuckle root, and 24 parts peppermint.

[0056] Experimental Example 1

[0057] 1. Experimental Materials

[0058] Helicobacter pylori, serial number 25923, was provided by the Department of Microbiology and Immunology, School of Basic Medical Sciences, Dali University. American giant salamander was provided by Professor Liu Heng of Dali University; honeysuckle, honeysuckle, and mint were purchased from the Dali Yaoca Market; lecithin Tween 80 nutrient agar medium and Helicobacter pylori culture (sheep blood) were provided by Haibo Biotechnology Co., Ltd., Dali High-tech Industrial Park.

[0059] Electric thermostatic water bath HH-1 (Changzhou Ronghua Instrument Manufacturing Co., Ltd.), digital display forced-air drying oven GZX-9140MBE (Shanghai Boxun Industrial Co., Ltd. Medical Equipment Factory), mold incubator MJX-100B-Z (Shanghai Boxun Industrial Co., Ltd. Medical Equipment Factory).

[0060] 2. Antibacterial effect experiment

[0061] Twenty volunteers (aged 25-45) were recruited and divided into two groups: a test group (n=10) and a control group (n=10). In the test group, oral samples were taken from the mouth before using the mouthwash, followed by rinsing with 15 mL of the herbal composition from Example 17 for 60 seconds, and then samples were taken again for testing. In the control group, water was used instead of the herbal composition for rinsing.

[0062] The specific sampling method is as follows: Use a sterile cotton swab to collect a sample from the left or right half of the back of the tongue. Place the swab on the midline of the back of the tongue, and then roll the swab from the back towards the tip of the tongue and then towards the outer edge of the tongue approximately four times to collect the sample. Place the swab from the left or right side of the tongue into a test tube containing 1 mL of PBS. Vortex the PBS tube containing the sample for 60 seconds, and then serially dilute it with PBS to a concentration of 10:10. -4 The diluted solution was inoculated onto lecithin Tween 80 nutrient agar medium and incubated at 36°C for 48 hours. The colony count of each plate was recorded.

[0063] SPSS 19.0 was used for statistical processing of the data. Paired-samples t-test was used to compare the experimental group and the control group. The results are expressed as mean ± standard deviation (X±S).

[0064] 3. Effect test on inhibiting Helicobacter pylori

[0065] Helicobacter pylori was prepared into colonies with a total bacterial count of 1.0 × 10⁻⁶. 4 CFU / mL~9.0×10 4 The bacterial suspension at CFU / mL was subjected to antibacterial testing using the suspension quantification method in WS / T650-2019 "Evaluation Methods for Antibacterial and Bacteriostatic Effects".

[0066] 4. Experimental Results

[0067] 4.1 Inhibit oral bacteria

[0068] The results of the experiment on the inhibition rate of oral bacteria by the traditional Chinese medicine composition are shown in Table 2. As shown in Table 2, there was no significant difference in the total number of oral bacterial colonies between the test subjects before using the traditional Chinese medicine composition of Example 17 and the control group (P>0.05); however, the bacterial reduction rates after using the traditional Chinese medicine composition of Example 17 and rinsing with water were 99.55% and 4.13%, respectively, showing a high degree of statistical significance (P<0.01). Therefore, this traditional Chinese medicine composition has a significant antibacterial effect on oral bacteria.

[0069] Table 2. Inhibition rate of traditional Chinese medicine composition on oral bacteria (n=10)

[0070]

[0071] 4.2 Effect of inhibiting Helicobacter pylori

[0072] As can be seen from the results in Table 3, the traditional Chinese medicine composition of Example 17 has an inhibition rate of more than 90% against Helicobacter pylori, and has a strong antibacterial effect according to Article 14 of the "Regulations on the Management of Labels and Instructions for Disinfection Products".

[0073] Table 3. Inhibition rate of traditional Chinese medicine composition against Helicobacter pylori

[0074]

Claims

1. A traditional Chinese medicine composition for inhibiting oral bacteria, wherein the raw materials of the traditional Chinese medicine composition are made from the following components in parts by weight: 27 parts of American cockroach, 39 parts of honeysuckle, 21 parts of honeysuckle root, and 24 parts of peppermint. The traditional Chinese medicine composition also includes sodium carboxymethyl cellulose, dextrin, starch, glycerol, or citric acid; The traditional Chinese medicine composition is selected from capsules, micro-pellets, powders, tablets, granules, solutions, emulsions, or suspensions.

2. The method for preparing the traditional Chinese medicine composition according to claim 1, comprising the following steps: 1) Take the whole dried American cockroach, add an appropriate amount of petroleum ether to extract, defatted, and dried at low temperature to obtain the defatted dried body of American cockroach; 2) Thoroughly crush honeysuckle, honeysuckle, and mint, mix with defatted dried American cockroach, add an appropriate amount of water, heat and extract using a multi-functional extraction tank with the temperature set, and filter with a 400-mesh filter cloth. 3) Collect the filtrate, concentrate it into an extract, and add excipients to prepare the final product; In step 1), the weight of petroleum ether added is 5-20 times that of dried American cockroaches, and the extraction is performed 3-5 times for a total extraction time of 12-36 hours. In step 2), the weight of water added should be 6-16 times that of the raw medicinal materials, and the extraction should be performed 2-5 times for 1-4 hours. In step 3), the filtrate is concentrated into an extract with a density of 1.20-1.40 g / mL.

3. The use of the traditional Chinese medicine composition according to claim 1 in the preparation of a drug for inhibiting oral bacteria.

4. The use of the traditional Chinese medicine composition according to claim 1 in the preparation of a drug for inhibiting Helicobacter pylori.

Images