High-throughput capillary-driven microfluidic chip for biochemical detection

Abstract

The application discloses a high-throughput capillary-driven microfluidic chip for biochemical detection, which comprises a first feeding unit, a first mixing microchannel, a first capillary pump, a first capillary trigger valve, a third feeding port, a second capillary pump and a detection chamber; the first feeding unit is used for adding detection raw materials and a lysis solution to the chip, and the outlet of the first feeding unit is connected with the inlet of the first mixing microchannel through a microchannel; and the detection chamber is used for receiving a liquid to be detected to detect the liquid. Through comprehensive improvement on the depth of the microchannel, the microcolumn array of the capillary pump and the size of the microcolumn, the microfluidic chip has greater flow flux and more stable flow speed.

Description

Technical Field

[0001] This invention belongs to the field of microfluidics technology and relates to a high-throughput capillary-driven microfluidic chip for biochemical detection. Background Technology

[0002] Microfluidic chip technology, also known as laboratory-on-a-chip technology, is a scientific technology characterized by the manipulation of fluids in a micrometer-scale space. Microfluidic chip technology is one of the most important cutting-edge scientific fields of the 21st century, possessing advantages such as low sample consumption, high throughput, integrability, and ease of fabrication and portability. Therefore, it is widely used in fields such as biology, environment, and chemical engineering.

[0003] The key to the normal operation of microfluidic chips lies in the precise control of fluid flow, displacing an accurate volume of liquid at a precise flow rate. In practice, two common methods are used: one is an active method using an external driving source, such as an optical field, electric field, magnetic field, or mechanical pump; the other is a passive method relying on the capillary force of the fluid itself. The advantage of the active driving method is that it can more precisely control the fluid flow and obtain more stable results. The disadvantage is that it requires many external control devices, significantly increasing production costs and imposing certain requirements on the application environment.

[0004] Currently, to increase throughput, an external injection pump is typically used to provide power. However, capillary microfluidic chips without external force drive struggle to maintain stable and high-speed fluid flow. To improve high-speed fluid flow in capillary microfluidic chips without external force drive, 202410150921X discloses a self-flowing pump-free microfluidic chip based on capillary action. This chip consists of several layers, with a drainage chamber connected to the driving chamber in the upper PDMS layer to enhance high-speed fluid flow within the chip. This structure is complex and has high manufacturing costs. Summary of the Invention

[0005] To address the aforementioned issues, the present invention aims to provide a high-throughput capillary-driven microfluidic chip for biochemical detection. Unlike existing microfluidic chips operating at the micrometer to tens of micrometer level, this microfluidic chip incorporates microchannels at the hundred-micrometer level and capillary pumps at the hundred-micrometer level. Through comprehensive improvements to the microchannel depth, capillary pump micropillar array, and micropillar size, this microfluidic chip achieves greater flow throughput and more stable flow velocity.

[0006] This invention is achieved through the following technical solution: A high-throughput capillary-driven microfluidic chip for biochemical detection includes a first feeding unit, a first mixing microchannel, a first capillary pump, a first capillary trigger valve, a third feed port, a second capillary pump, and a detection chamber; The first feeding unit is used to add detection raw materials and pyrolysis solution to the chip. The outlet of the first feeding unit is connected to the inlet of the first mixing microchannel through a microchannel. The first mixing microchannel is used to provide a mixing microchannel for the detection raw material and the lysis solution, and to provide a reaction site for them. The outlet of the first mixing microchannel is connected to the inlet of the first capillary pump through the microchannel. The microchannel is a hundred-micrometer-level channel with a depth of 0.1~0.6 mm, a width range of 0.2~1 mm, and a width-to-depth ratio of 2-5:1. The first capillary pump is at the hundred-micron level, with a length of 10-21 mm, a width of 5-10 mm, and a height of 0.1-0.6 mm. The height is consistent with the depth of the microchannel, and the short axis of the microcolumn is 100-750 μm. The outlet of the first capillary pump is connected to the first capillary trigger valve through the microchannel. The second inlet of the first capillary trigger valve is connected to the outlet of the third feed port through a microchannel, and the outlet of the first capillary trigger valve is connected to the inlet of the second capillary pump through a microchannel. The second capillary pump is in the hundreds of micrometers range, with a length of 10-21 mm, a width of 5-10 mm, and a height of 0.1-0.6 mm. The height is consistent with the depth of the microchannel. The short axis of the microcolumn is 100-750 μm. The outlet of the second capillary pump is connected to the inlet of the detection chamber. The testing chamber receives and tests the liquid to be tested. During testing, in the first stage, when the liquid flows through the first mixing microchannel and the first capillary pump: the hundred-micron-sized microchannel, under the influence of capillary force, gravity, and viscosity, combined with the pumping action of the hundred-micron-sized first capillary pump, greatly increases the flow rate and maintains a good flow velocity. In the second stage, after feeding from the third inlet, when the liquid passes through the second capillary pump, the hundred-micron-sized microchannel, under the influence of capillary force, gravity, and viscosity, combined with the pumping action of the hundred-micron-sized second capillary pump, greatly increases the flow rate and maintains a good flow velocity, resulting in a large flow rate and good flow velocity of the liquid throughout the chip system.

[0007] It also includes a second capillary trigger valve and a reagent inlet. The first outlet of the second capillary trigger valve is connected to the inlet of the reagent inlet, the second outlet of the second capillary trigger valve is connected to the detection chamber, and the inlet of the second capillary trigger valve is connected to the outlet of the second capillary pump.

[0008] The feeding unit includes a first feed inlet and a second feed inlet. Both the first feed inlet and the second feed inlet are located on the inlet side of the first capillary pump. The first feed inlet and the second feed inlet are connected through a T-shaped mixing port. The outlet of the T-shaped mixing port is connected to the inlet of the first mixing microchannel. The outlet of the first mixing microchannel is connected to the first capillary pump. During detection, the first feed inlet and the second feed inlet can be fed simultaneously, and the liquids entering from the first feed inlet and the second feed inlet respectively are mixed in the T-shaped mixing port.

[0009] It also includes a second mixing microchannel. The inlet of the second mixing microchannel is connected to the outlet side of the first capillary trigger valve, and the outlet of the second mixing microchannel is connected to the inlet of the second capillary pump. When the liquid reflows through the second capillary trigger valve, the hundred-micron-sized microchannel, combined with the pumping action of the hundred-micron-sized second capillary pump, greatly increases the flow rate and maintains a good flow velocity under the combined action of capillary force, gravity, and viscous force, resulting in a large flow rate and good flow velocity of the liquid throughout the chip system. The width and depth of the microchannel are the same as those of the first and second mixing microchannels.

[0010] The capillary pump configuration in this invention differs from conventional capillary pumps. From the perspective of micro-column size and arrangement, it explores the optimal micro-column capillary pump structure on a large spatial scale, and measures show that the maximum liquid flow rate under this structure is tens to hundreds of times that of conventional pumps, exhibiting a significant difference in flow characteristics compared to small-sized micro-column capillary pumps. However, the height of the capillary pump and the size of the capillary micro-columns in this invention cannot be increased indefinitely, as this would affect the filling effect and the stability of the pumped fluid, while also increasing the flow path resistance. In complex and diverse biochemical detection environments, the liquid and microchannel are not only pulled by capillary forces, but also by inertial forces due to gravity. The flow patterns of liquid in small-sized microchannels and capillary pumps differ from those in large-sized microchannels. This invention increases the depth of the microchannel, thereby increasing the gravity and inertial forces of the liquid per unit length. Combined with the drag-reducing and flow-guiding effects of the capillary pump, the shear force of the microchannel wall on the liquid is further reduced. This results in a chip with a large flow throughput. In other words, this invention greatly improves the flow throughput of the chip through the synergistic effect of a microchannel depth of hundreds of micrometers and a capillary pump of hundreds of micrometers.

[0011] In practice, the mixing microchannels in this invention serve as transport units while also providing sufficient spacetime for the reaction. In practice, serpentine channels or similar methods can be used to enhance fluid mixing. The required mixing unit length varies depending on the properties of the fluid used.

[0012] The first capillary pump uses rhomboid micropillars, arranged in two or four horizontal rows along its length. In practice, these two arrangements have shown to minimize resistance and optimize the structure of the capillary pump. The shape and arrangement of the internal micropillars can be modified to suit different applications, as long as the basic design principles are met.

[0013] In one embodiment, the first capillary pump is long L =10.5 mm, width W =5mm, capillary microcolumn minor axis length D =0.5 mm, length of capillary microcolumn LD =1mm, longitudinal distance of micropillars SD =0.5mm, lateral distance of micropillars FD =0.5 mm.

[0014] A detection method for a high-throughput capillary-driven microfluidic chip includes a sample entering through a first inlet and a lysis buffer entering through a second inlet. The two samples meet at a T-shaped mixing port and flow through a microchannel for fluid delivery to a first mixing microchannel for mixing and reaction. The lysis buffer then flows into a first capillary pump for high-throughput pumping. The lysed sample flows through the microchannel for fluid delivery to a first capillary trigger valve where it is stopped. The amplification buffer enters through a third inlet and flows to the first capillary trigger valve, causing the lysed sample to flow again and mix with the lysis buffer in a second mixing microchannel. The amplified sample then flows to a second capillary pump for high-throughput pumping. The amplified sample flows to the second capillary trigger valve where it is stopped. The detection buffer enters through a fourth inlet and flows to the second capillary trigger valve, causing the amplified sample to flow again and mix with the detection buffer before entering the detection chamber.

[0015] Compared with the prior art, the present invention has the following advantages and beneficial effects: 1. This invention sets up microchannels and capillary pumps at the level of hundreds of micrometers. Through comprehensive improvements in the depth of the microchannels, the arrangement of the capillary pump micropillar array, and the size of the micropillars, the microfluidic chip has a larger flow throughput and a more stable flow velocity, making it a fully automated capillary microfluidic detection system. 2. The overall system throughput and the throughput of the locally simplified simulation system of the present invention are both higher than the throughput of the individual capillary pumps in the prior art, indicating that the overall system throughput of the present invention is greatly improved. 3. This invention can design multiple detection units to perform different detection reactions, and the results do not interfere with each other. The structure that achieves high throughput, fast and stable performance is designed to meet the needs of multiple reactions.

[0016] 4. The processing cost of this invention is low, making it suitable for large-scale production applications. Attached Figure Description

[0017] The accompanying drawings, which are included to provide a further understanding of embodiments of the invention and form part of this application, do not constitute a limitation thereof. In the drawings: Figure 1 This is a schematic diagram of the structure of the present invention.

[0018] Figure 2 This is a schematic diagram of the capillary pump structure of the present invention.

[0019] Figure 3 This is a schematic diagram of the capillary pump structure of the present invention.

[0020] Figure 4 This is a top view of the first capillary trigger valve.

[0021] Figure 5 This is a side view of the first capillary trigger valve.

[0022] The attached diagram shows the markings and corresponding component names: 1-First feed inlet, 2-Second feed inlet, 3-T-type mixing port, 4-Microchannel, 5-First mixing microchannel, 6-First capillary pump, 7-Third feed inlet, 8-First capillary trigger valve, 9-Second mixing microchannel, 10-Second capillary pump, 11-Second capillary trigger valve, 12-Fourth feed inlet, 13-Detection chamber, 14-Capillary microcolumn. Detailed Implementation

[0023] To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments and accompanying drawings. The illustrative embodiments and descriptions of the present invention are only used to explain the present invention and are not intended to limit the present invention.

[0024] Example 1

[0025] A high-throughput capillary-driven microfluidic chip for biochemical detection includes a first feeding unit, a first mixing microchannel 5, a first capillary pump 6, a first capillary trigger valve 8, a third feed port 7, a second capillary pump 10, and a detection chamber 13. The first feeding unit, also known as the raw material pyrolysis solution feeding unit, is used to add detection raw materials and pyrolysis solution to the chip. The outlet of the first feeding unit is connected to the inlet of the first mixing microchannel 5 through the microchannel 4. The first mixing microchannel 5 is used to provide a mixing microchannel for the detection raw material and the lysis solution, and to provide a reaction site for them. The outlet of the first mixing microchannel 5 is connected to the inlet of the first capillary pump 6 through the microchannel 4. The microchannel 4 is a channel of hundreds of micrometers, with a depth of 0.1~0.6 mm, a width of 0.2~1 mm, and a width-to-depth ratio of 2-5:1. The first capillary pump 6 is at the hundred-micron level, with a length of 10-21 mm, a width of 5-10 mm, and a height of 0.1-0.6 mm. The height is consistent with the depth of the microchannel 4, and the short axis of the microcolumn is 100-750 μm. The outlet of the first capillary pump 6 is connected to the first capillary trigger valve 8 through the microchannel 4. The second inlet of the first capillary trigger valve 8 is connected to the outlet of the third feed port 7 through the microchannel 4, and the outlet of the first capillary trigger valve 8 is connected to the inlet of the second capillary pump 10 through the microchannel 4. The second capillary pump 10 is in the hundreds of micrometers range, with a length of 10-21 mm, a width of 5-10 mm, and a height of 0.1-0.6 mm. The height is consistent with the depth of the microchannel 4, and the short axis of the microcolumn is 100-750 μm. It also includes a second capillary trigger valve 11 and a reagent inlet 12. The first inlet of the second capillary trigger valve 11 is connected to the outlet of the reagent inlet 12, the outlet of the second capillary trigger valve 11 is connected to the inlet of the detection chamber 13, and the second inlet of the second capillary trigger valve 11 is connected to the outlet of the second capillary pump 10.

[0026] Testing chamber 13 is used to receive the liquid to be tested and to test it.

[0027] The feeding unit includes a first feed inlet 1 and a second feed inlet 2. Both the first feed inlet 1 and the second feed inlet 2 are located on the inlet side of the first capillary pump 6. The first feed inlet 1 and the second feed inlet 2 are connected through a T-shaped mixing port 3. The outlet of the T-shaped mixing port 3 is connected to the inlet of the first mixing microchannel 5, and the outlet of the first mixing microchannel 5 is connected to the first capillary pump 6. During detection, feed can be simultaneously introduced from the first feed inlet 1 and the second feed inlet 2. The liquids entering from the first feed inlet 1 and the second feed inlet 2 respectively are mixed in the T-shaped mixing port 3. The first feed inlet 1 and the second feed inlet 2 can be filled with pyrolysis solution or raw material solution, respectively.

[0028] It also includes a second mixing microchannel 9, the inlet of which is connected to the outlet side of the first capillary trigger valve 8, and the outlet of the second mixing microchannel 9 is connected to the inlet of the second capillary pump 10.

[0029] During testing, the sample enters through the first inlet 1, and the lysis solution enters through the second inlet 2. The two converge at the T-shaped mixing port 3 and flow through the microchannel 4 to the first mixing microchannel 5 for mixing and reaction. Then, the sample flows into the first capillary pump 6 to achieve high-throughput pumping. The lysed sample flows through the microchannel 4 to the first capillary trigger valve 8, where it is stopped. The amplification solution enters through the third inlet 7 and flows to the first capillary trigger valve 8, causing the lysed sample solution to flow again and mix with it in the second mixing microchannel 4. Then, the sample flows into the second capillary pump 10 to achieve high-throughput pumping. The amplified sample flows to the second capillary trigger valve 11, where it is stopped. The detection solution enters through the fourth inlet and flows to the second capillary trigger valve 11, causing the amplified sample solution to flow again and mix with it before entering the detection chamber 13.

[0030] Specifically: First capillary pump 6 length L =10.5 mm, width W =5 mm, capillary micro-column 14 minor axis length D =0.5mm, longitudinal distance of micropillars SD =0.5mm, lateral distance of micropillars FD =2.5mm (the first capillary pump 6 and the second capillary pump 10 have the same structure); the length of the channel before the pump is 2mm. The micropillars of the first capillary pump 6 are rhomboid micropillars, and the micropillar array is arranged horizontally in two rows along the length of the first capillary pump 6. The microchannel 4 has a depth of 0.1mm and a width of 0.5mm. The chip uses a microchannel with a width-to-depth ratio of 5:1 to transport fluid, and uses a serpentine microchannel to save chip space and enhance mixing. The first capillary pump 6 and the second capillary pump 10 have the same structure.

[0031] A comprehensive simulation experiment was conducted using water as the simulated liquid. The results showed that the time for water to reach the first capillary trigger valve 8 was 5 seconds; the time from the breakthrough of the first capillary valve to the second capillary trigger valve 11 in the second stage was 3.73 seconds; and the time for detection after the water was added at the third inlet 7 was 0.02 seconds. The total filling flow rate, or flux, was 8.6 × 10⁻⁶. -9 m 3 / s.

[0032] The overall system throughput of the present invention is higher than that of a single capillary pump in the prior art, indicating that the overall system throughput of the present invention is greatly improved.

[0033] The use of the first capillary trigger valve 8 can effectively stop the fluid flow, and the depth of the channel before the valve... h 1 and the depth of the valve downstream channel h The ratio of 2 is 1:2, and fluid shut-off is achieved when the contact angle between the fluid and the wall is 67.5°. The first capillary trigger valve 8 and the second capillary trigger valve 11 have the same structure.

[0034] To simplify the experiment, water was used as the simulated liquid in this invention. A simplified flow simulation was performed by connecting the inlet, the first mixing microchannel 5, and the first capillary pump 6 via microchannel 4. By varying the aspect ratio of microchannel 4 and the micropillar array and size of the first capillary pump 6, the flux results of Examples 2-5 and Comparative Examples 1-4 were finally obtained. Note: An increase in local flux reflects an increase in the overall structure.

[0035] Example 2

[0036] like Figure 2 As shown, the microchannel 4 has a depth of 0.1 mm and a width of 0.4 mm. The chip uses a channel with a width-to-depth ratio of 4:1 to transport fluid.

[0037] The micropillars of the first capillary pump 6 are rhomboid micropillars, and the micropillar array is arranged horizontally in two rows (top and bottom) along the length of the first capillary pump 6. A simulation experiment was conducted using a capillary pump with rhomboid micropillars (the simulated liquid was water). The capillary pump length... L =10.5 mm, width W =5 mm, capillary micro-column 14 minor axis length D =0.5 mm, capillary micro-pillar 14 major axis length LD =1 mm, longitudinal distance of micropillars SD=0.5 mm, lateral distance of micropillars FD When the diameter is 0.5 mm and the length of the inlet channel is 2 mm, the flow resistance of the pump is 9.766 × 10⁻⁶ mm. 8 Pa·s / m 3 The flow rate is 7.688 × 10 -8 m 3 / s.

[0038] Example 3

[0039] like Figure 3 As shown, the channel size and sample addition and flow order are the same as in Example 2, except that the depth of microchannel 4 is 0.2 mm and the width is 1 mm, and the chip uses a channel with a width-to-depth ratio of 5:1 to transport fluid.

[0040] A capillary pump with rhomboid micropillars was used in the simulation experiment (the simulated liquid was water). The capillary pump was long. L =21mm, width W =10 mm, capillary micro-column 14 minor axis length D =0.5 mm, capillary micro-pillar 14 major axis length LD =1 mm, longitudinal distance of micropillar SD =0.5 mm, lateral distance of micropillars FD When the diameter is 0.5 mm and the length of the inlet channel is 2 mm, the flow resistance of the pump is 8.079 × 10⁻⁶ mm. 8 Pa·s / m 3The flow rate is 4.284 × 10 -8 m 3 / s.

[0041] Example 4

[0042] The channel dimensions and sample addition and flow sequence are the same as in Example 2, except that the microchannel 4 has a depth of 0.2 mm and a width of 1 mm, and the chip uses a channel with a width-to-depth ratio of 5:1 to transport the fluid. A capillary pump with rhomboid micropillars was used for simulation experiments (the simulated liquid was water). The capillary pump length... L =10.5 mm, width W =5 mm, capillary micro-column 14 minor axis length D =0.5 mm, capillary micro-pillar 14 major axis length LD =1 mm, longitudinal distance of micropillar SD =0.5 mm, lateral distance of micropillars FD =0.5 mm, and the length of the inlet channel is 18 mm, the flow resistance of the pump is 5.446 × 10. 8 Pa·s / m 3 The flow rate is 1.588 × 10 -8 m 3 / s.

[0043] Example 5

[0044] A capillary pump with rhomboid micropillars was used in the simulation experiment. The capillary pump length was... L =10.5 mm, width W =5 mm, capillary micro-column 14 minor axis length D =0.5mm, longitudinal distance of micropillars SD =0.5mm, lateral distance of micropillars FD When the diameter is 2.5mm and the length of the channel before the pump is 2mm, the flow resistance of the pump is 5.732×10. 9 Pa·s / m 3 The flow rate is 7.507 × 10 -8 m 3 / s, compared to the 2.3 × 10⁻⁶ flux of the capillary pump (simulated by water flow) found in the literature. -9 m 3 / s increased by 32.6 times.

[0045] Comparative Example 1 Similar to Example 2, the difference is that the depth of microchannel 4 is 40 micrometers. The final measured flow rate of the capillary pump was 2.3 × 10⁻⁶. -9 m 3 / s.

[0046] Comparative Example 2 Similar to Example 2, the difference lies in the size of the capillary pump micropillars, which are on the order of tens of micrometers, specifically with a minor axis length of 50 μm and a length of 100 μm. The final measured flow resistance of the capillary pump was 0.78 × 10⁻⁶. 14 Pa·s / m 3 The flow rate is 4.11 × 10 -11 m 3 / s.

[0047] Comparative Example 3 Similar to Example 2, the difference is that the channel depth is 6 micrometers, and the aspect ratio is 50:3. The final measured flow resistance of the capillary pump is 1.23 × 10⁻⁶. 14 Pa·s / m 3 The flow rate is 3.42 × 10 -11 m 3 / s.

[0048] Comparative Example 4 Similar to Example 2, the difference is that the channel depth is 40 micrometers and the aspect ratio is 25:4. The final measured flow rate of the capillary pump is 1.82 × 10⁻⁶. -9 m 3 / s.

[0049] In the above embodiments, through the simplified simulated water experiment, it is shown that the flux of the simplified simulated structure of the present invention is greater than that of the single capillary pump in the prior art. Examples 2 and Comparative Examples 1-4 show that different aspect ratios and capillary pumps of different sizes and array distributions have different effects on the flux of the structure. Under the synergistic effect of the microchannel 4 at the hundred-micron level and the capillary pump at the hundred-micron level set in the present invention, the flow flux of the present invention is greatly improved, and the flux of the entire system is also greatly improved.

[0050] The specific embodiments described above further illustrate the purpose, technical solution, and beneficial effects of the present invention. It should be understood that the above description is only a specific embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A high-throughput capillary-driven microfluidic chip for biochemical detection, characterized in that, It includes a first feeding unit, a first mixing microchannel (5), a first capillary pump (6), a first capillary trigger valve (8), a third feed port (7), a second capillary pump (10), and a detection chamber (13); The first feeding unit is used to add samples and lysis solution to the chip. The outlet of the first feeding unit is connected to the inlet of the first mixing microchannel (5) through the microchannel (4). The first mixing microchannel (5) is used to provide a mixing microchannel for the sample and lysis solution and to provide a reaction site for them. The outlet of the first mixing microchannel (5) is connected to the inlet of the first capillary pump (6) through the microchannel (4). The first capillary pump (6) is at the hundred-micrometer level. The length of the first capillary pump (6) is 10-21 mm, the width is 5-10 mm, and the height is 0.1-0.6 mm. The height is consistent with the depth of the microchannel (4). The capillary micropillars (14) of the first capillary pump (6) are rhomboid micropillars, and the array of capillary micropillars (14) is arranged in two or four rows horizontally along the length of the first capillary pump (6). The minor axis of the capillary micropillars (14) is long. D =0.1~0.75 mm, capillary micro-column (14) major axis length LD =0.1~1.5 mm, longitudinal distance of capillary microcolumns (14) SD =0.5~2 mm, lateral distance of capillary microcolumns (14) FD =0.5~2.5 mm; The outlet of the first capillary pump (6) is connected to the first capillary trigger valve (8) through the microchannel (4); The second inlet of the first capillary trigger valve (8) is connected to the outlet of the third feed port (7) through the microchannel (4). The third feed port (7) is used for the entry of the amplification solution. The outlet of the first capillary trigger valve (8) is connected to the inlet of the second capillary pump (10) through the microchannel (4). The second capillary pump (10) has the same structure as the first capillary pump (6), and the outlet of the second capillary pump (10) is connected to the inlet of the detection chamber (13); the microchannel (4) and the first mixing microchannel (5) have the same width and depth; the microchannel (4) is a channel of hundreds of micrometers, with a depth of 0.1~0.6 mm, a width range of 0.2~1 mm, and a width-to-depth ratio of 2-5:1; The testing chamber (13) is used to receive the liquid to be tested and to test it; It also includes a second capillary trigger valve (11) and a test reagent inlet (12). The first inlet of the second capillary trigger valve (11) is connected to the outlet of the test reagent inlet (12). The outlet of the second capillary trigger valve (11) is connected to the test chamber (13). The second inlet of the second capillary trigger valve (11) is connected to the outlet of the second capillary pump (10).

2. The high-throughput capillary-driven microfluidic chip for biochemical detection according to claim 1, characterized in that, The first feeding unit includes a first feed port (1) and a second feed port (2). The first feed port (1) and the second feed port (2) are both located on the inlet side of the first capillary pump (6). The first feed port (1) and the second feed port (2) are connected through a T-shaped mixing port (3). The outlet of the T-shaped mixing port (3) is connected to the inlet of the first mixing microchannel (5). The outlet of the first mixing microchannel (5) is connected to the first capillary pump (6). During operation, the first feed port (1) and the second feed port (2) feed at the same time. The liquids entering from the first feed port (1) and the second feed port (2) respectively are mixed in the T-shaped mixing port (3).

3. The high-throughput capillary-driven microfluidic chip for biochemical detection according to claim 1, characterized in that, It also includes a second mixing microchannel (9), the inlet of which is connected to the outlet side of the first capillary trigger valve (8), and the outlet of the second mixing microchannel (9) is connected to the inlet of the second capillary pump (10). The second mixing microchannel (9) and the microchannel (4) have the same width and depth.

4. The high-throughput capillary-driven microfluidic chip for biochemical detection according to claim 1, characterized in that, First capillary pump (6) long L =10~10.5 mm, width W =5~10 mm.

5. The detection method for a high-throughput capillary-driven microfluidic chip according to claim 3, characterized in that, The sample enters from the first inlet (1), and the lysis solution enters from the second inlet (2). The two meet at the T-type mixing port (3). After flowing through the microchannel (4) of the conveying fluid to the first mixing microchannel (5) for mixing reaction, the sample flows into the first capillary pump (6) to achieve high-throughput pumping of the fluid. The lysed sample flows through the microchannel (4) of the conveying fluid to the first capillary trigger valve (8) and is then stopped. The amplification solution enters from the third inlet (7), flows to the first capillary trigger valve (8) to trigger the lysed sample to flow again and mix with it in the second mixing microchannel (9). The sample then flows into the second capillary pump (10) to achieve high-throughput pumping of the fluid. The amplified sample flows to the second capillary trigger valve (11) and is then stopped. The detection solution enters from the detection reagent inlet (12), flows to the second capillary trigger valve (11) to trigger the amplified sample to flow again and mix with it before entering the detection chamber (13).

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