A device and method for studying the approach behavior of grain storage pests

Abstract

The application belongs to the field of stored grain pest control, and discloses a device and method for studying the tendency behavior of stored grain pests, which comprises a reaction box, the reaction box comprises a box body and a box cover, a inoculation hole is arranged in the middle of the box cover, two first partition plates are arranged in the reaction box, the reaction box is divided into an observation area and two collection areas by the two first partition plates, the two collection areas are respectively arranged on the two sides of the observation area, and the inoculation hole is arranged above the observation area, and at least one discharge port is arranged in each of the observation area and the two collection areas. The position of the first partition plate can be adjusted to adjust the position and space size of the observation area and the collection area, the device can be used for studying the tendency behavior of different stored grain pests, the collection objects in the collection areas can be prevented from entering the observation area, the device does not affect the passing of the stored grain pests, and the tendency behavior of the stored grain pests can be observed.

Description

Technical Field

[0001] This invention belongs to the field of grain storage pest control technology, and in particular relates to a device and method for studying the directional behavior of grain storage pests. Background Technology

[0002] Not only do stored grain pests cause weight loss, quality decline, heat generation, and mold growth in stored grains, but their excrement and waste can also reduce the processing quality, seed quality, and technological quality of stored grains. They can even cause the spread of diseases and harm people's physical and mental health.

[0003] Currently, chemical control is the main method for controlling stored grain pests, using fumigation to achieve the desired control. However, due to the irrational use of fumigation, stored grain pests are increasingly developing resistance to pesticides, leading to increased resistance and reduced control effectiveness. Mechanical control technology utilizes the specific attraction and habits of stored grain pests, applying simple tools and physical technologies such as air, light, and electricity to control them. It has the advantages of being simple, effective, and environmentally friendly. Stored grain pest traps are a common mechanical control method, not only for trapping and monitoring stored grain pests in grain piles but also for ecological research on stored grain pests. However, existing stored grain pest traps are mostly used for breeding pests or observing their movement and behavior; devices for studying the tactical behavior of stored grain pests are not common, and observation and statistical analysis during the research process are inconvenient. Summary of the Invention

[0004] To address the problem that existing grain pest traps cannot be used to study the directional behavior of grain pests, this invention proposes a device and method for studying the directional behavior of grain pests. This overcomes the inconvenience of detecting grain pests and can not only study the directional behavior of grain pests towards different attractants, but also analyze the effective attraction distance of the attractants on grain pests, thereby simulating the attraction effect of different attractants on grain pests in actual warehouses.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0006] A device for studying the tactic behavior of stored grain pests includes a reaction chamber, which is a hollow box. The reaction chamber includes a box body and a lid, with the lid located on the upper part of the box body. An inoculation hole is provided in the middle of the lid. Two first partition plates are provided inside the reaction chamber, dividing the interior of the reaction chamber into an observation area and two trapping areas. The two trapping areas are respectively distributed on both sides of the observation area, and the inoculation hole is located above the observation area. Each of the observation area and the two trapping areas is provided with at least one discharge port.

[0007] Using the above scheme, the position and size of the observation area and the trapping area inside the device can be adjusted as needed through the first partition plate, which facilitates the study of the directional behavior of different types of stored grain pests.

[0008] Furthermore, each of the trapping zones is provided with at least one second partition plate, and the second partition plates in the two trapping zones are arranged symmetrically from left to right. The second partition plate divides the interior of each trapping zone into multiple grain storage spaces; each grain storage space is provided with a discharge port.

[0009] Alternatively, no second partition can be installed in the trapping area, and the trapping area can be used directly as grain storage space.

[0010] By adopting the above-mentioned further scheme, the trapping area is divided into multiple grain storage spaces, and the effects of long-distance crawling on the directional behavior of stored pests can be investigated while conducting control or comparative experiments.

[0011] Furthermore, each discharge port is equipped with a discharge box, which includes a collection hopper and a sealing plug. The collection hopper has a conical structure that is larger at the top and smaller at the bottom, and the bottom of the collection hopper has a discharge port. The sealing plug is used to block the discharge port.

[0012] The above-mentioned further solution involves setting a discharge box at the discharge port and designing the collection hopper of the discharge box as a conical structure that is larger at the top and smaller at the bottom, which facilitates the release of attractants and stored grain pests from the reaction chamber.

[0013] Furthermore, a humidity regulating box is also provided in the trapping area. The humidity regulating box includes a box body, which is a hollow box body. The top of the box body has several humidity regulating holes smaller than the particle size of the attractant and the width of the grain pest. The side wall of the box body is provided with hooks, and the side wall of the box body is provided with a corresponding card plate. The hooks and card plates are detachably connected.

[0014] With the above-mentioned further solution, the humidity regulating box can be detachably connected to the box body through hooks and plates, and is easy to disassemble, making it convenient to replace or add salt water inside the box. In addition, the humidity regulating box can be connected to the trapping area, which can roughly regulate the humidity in the trapping area.

[0015] The trapping area can also employ existing temperature and humidity control devices to control the temperature and humidity inside the box.

[0016] Furthermore, the inner wall of the box is provided with a plurality of connecting grooves, and a first partition plate or a second partition plate is movably arranged in each connecting groove, or a portion of the connecting grooves are movably arranged with a first partition plate or a second partition plate; the plurality of connecting grooves are arranged at equal intervals.

[0017] Furthermore, the first partition plate includes a partition frame and a partition mesh, with the partition mesh fixedly disposed in the middle of the partition frame; the second partition plate has the same structure as the first partition plate.

[0018] By adopting the above-mentioned further solution, the partition frame can better support the partition net and provide sufficient strength for the partition frame. At the same time, by movably setting the partition frame in the connecting groove, it can better support the partition plate and effectively prevent the partition net from deforming and failing when filling with attractant.

[0019] Furthermore, the aperture of the separating mesh is greater than or equal to the body width of the stored grain pests and smaller than the particle size of the attractant.

[0020] By adopting the above-mentioned further scheme, the restriction of the mesh size ensures that the mesh does not block stored grain pests, but it can block the attractants, allowing stored grain pests to enter the attractant area from the observation area. This facilitates experiments on the directional behavior of stored grain pests towards different attractants. Furthermore, stored grain pests can move freely within the attractant area, which also facilitates experiments on the influence of long-distance crawling on the directional behavior of stored grain pests.

[0021] Furthermore, two rows of guide rails are arranged parallel to each other on the top of the box, and the box cover is slidably connected to the box through the guide rails; or one end of the box cover is hinged to the box, and the other end is snapped to the box or directly covers the surface of the box; or the box cover directly covers the surface of the box.

[0022] The reaction chamber also includes a cover for sealing the inoculation hole, the cover being adapted to the inoculation hole and detachably connected.

[0023] The above-mentioned further scheme facilitates the disassembly and fixing of the box lid and the box body, thereby making it easier to place the attractant in the attraction area; and by setting a cover, the inoculation hole can be sealed after inoculating the stored grain pests, preventing the stored grain pests in the observation area from escaping, while also reducing the influence of external factors on the experimental results.

[0024] A method for studying the tactic behavior of stored grain pests includes the following steps:

[0025] Step 1: Determine the position of the first partition plate according to the predetermined movement distance of stored grain pests, and use the first partition plate to divide the reaction box into an observation area and a trapping area;

[0026] Step 2: Design the location and quantity of grain storage space according to experimental needs;

[0027] No second partition is installed in the trapping area; the trapping area is directly used as grain storage space.

[0028] Alternatively, at least one second partition can be set up in each trapping area to divide each trapping area into at least multiple grain storage spaces;

[0029] Step 3: Place attractants into one or more grain storage spaces in one side of the attractant area, and place equal amounts of different attractants into grain storage spaces in symmetrical positions in the other side of the attractant area, or place equal amounts of the same attractants into grain storage spaces in asymmetrical positions in the other side of the attractant area, with the top surface of the attractants flat.

[0030] Step 4: Place the reaction chamber in a dark environment and let it stand. Inoculate the observation area with 30 to 120 stored grain pests and control the temperature and relative humidity during the experiment.

[0031] Step 5: After an interval of 2 to 8 hours, discharge the attractants from the outlet of each grain storage space, count the number of stored grain pests, and record the directional data of the stored grain pests.

[0032] Using the above technical solution, experiments can be conducted on the attraction behavior of stored grain pests to different attractants, while also studying the impact of long-distance crawling on the attraction behavior of stored grain pests. The same device can be used to study multiple experiments affecting the attraction behavior of stored grain pests.

[0033] Furthermore, before step 3, the process includes placing a humidification box filled with saline solution inside the reaction chamber.

[0034] The above-mentioned further technical solutions facilitate the maintenance of humidity in the trapping area during the test in excessively dry test environments.

[0035] The beneficial effects of the present invention through the above technical solution are as follows:

[0036] 1. By adjusting the position of the first partition, the position and size of the observation area and the trapping area can be adjusted. This can be used to study the tactic behavior of different stored grain pests and prevent the attractants in the trapping area from entering the observation area, but it will not affect the passage of stored grain pests and allow for observation of their tactic behavior.

[0037] 2. The trapping area is set on both sides of the observation area. As needed, a control experiment can be conducted by placing the trapping substance at one end and the control substance at the other end, or a comparative experiment can be conducted by placing different trapping substances at both ends.

[0038] 3. The trapping area can be used directly as a grain storage space or divided into multiple grain storage spaces by a second partition. While conducting experiments on the attraction behavior of grain storage pests to different traps, it is also possible to analyze the effective trapping distance of different traps for grain storage pests. In addition, experiments can be conducted to explore the effective trapping distance of a single trap for grain storage pests, making it highly practical.

[0039] 4. The humidity control box inside the reaction chamber is used to regulate the humidity in the corresponding trapping area to ensure the humidity of the traps and reduce the impact of humidity on the test results. Each grain storage space and observation area is equipped with a discharge port and discharge box to facilitate the discharge of the traps after the test and to count the number of stored grain pests in the traps.

[0040] 5. This invention has a simple structure, is highly efficient and environmentally friendly, and has low testing costs. It can not only test the tactic behavior of stored grain pests towards different attractants, overcoming the shortcomings of existing devices that are inconvenient for detecting stored grain pests, but also simulate the attraction effect of different attractants on stored grain pests in actual warehouses. It has important theoretical research and application value. Attached Figure Description

[0041] Figure 1 This is a schematic diagram of the structure of a grain storage pest directional behavior research device according to the present invention.

[0042] Figure 2 This is a front view of a device for studying the directional behavior of stored grain pests according to the present invention.

[0043] Figure 3 This is a side view of a device for studying the directional behavior of stored grain pests according to the present invention.

[0044] Figure 4 This is a top view of the internal structure of a grain storage pest directional behavior research device according to the present invention.

[0045] Figure 5 This is a schematic diagram of the discharge box in a grain storage pest tactic behavior research device of the present invention;

[0046] Figure 6 This is a schematic diagram of the partition plate in the grain storage pest tactic behavior research device of the present invention;

[0047] Figure 7 This is a schematic diagram of the installation of the partition plate in the grain storage pest tactic behavior research device of the present invention.

[0048] Figure 8 This is a schematic diagram of the humidity control box in a grain storage pest tactic behavior research device of the present invention;

[0049] Figure 9 This is a schematic diagram of the sealing block structure in a grain storage pest directional behavior research device of the present invention;

[0050] Figure 10 This invention relates to a device for studying the directional behavior of stored grain pests. Figure 1 A magnified view of part A in the image;

[0051] Figure 11 This is a schematic diagram of the discharge box in Embodiment 3 of the grain storage pest tactic behavior research device of the present invention;

[0052] The attached diagram is labeled as follows: 1 is the reaction chamber; 2 is the observation area; 3 is the induction area; 401 is the first partition plate; 402 is the second partition plate; 5 is the humidity control box; 6 is the discharge box; 7 is the partition frame; 8 is the partition net; 9 is the connecting groove; 10 is the collection hopper; 11 is the discharge port; 12 is the sealing plug; 13 is the box body; 14 is the hook; 15 is the clamping plate; 16 is the box body; 17 is the box cover; 18 is the inoculation hole; 19 is the sealing cover; 20 is the support foot; 21 is the guide rail; 22 is the humidity control hole; 23 is the insertion plate. Detailed Implementation

[0053] like Figures 1 to 11 As shown, this invention relates to a device for studying the tactic behavior of stored grain pests, comprising a reaction chamber 1, which is a hollow box. The reaction chamber 1 includes a box body 16 and a box cover 17. The box cover 17 is located on the upper part of the box body 16, and an inoculation hole 18 is provided in the middle of the box cover 17. Two first partition plates 401 are provided inside the reaction chamber 1, which divide the interior of the reaction chamber 1 into an observation area 2 and two trapping areas 3. The two trapping areas 3 are respectively distributed on both sides of the observation area 2, and the inoculation hole 18 is located above the observation area 2. Each of the observation area 2 and the two trapping areas 3 is provided with at least one discharge port.

[0054] In this invention, the observation area 2 is used to inoculate stored grain pests, and the trapping area 3 contains attractants to attract stored grain pests. After the reaction chamber 1 is divided by the first partition plate 401, stored grain pests are inoculated into the reaction chamber 1 from the observation area 2. Under the attraction of the attractants, the stored grain pests will crawl to the corresponding trapping area.

[0055] In this invention, the position and size of the observation area 2 and the attraction area 3 inside the reaction chamber 1 can be adjusted accordingly by the placement of the first partition plate 401, thereby adapting to the activity characteristics of different stored grain pests. This prevents the inability to distinguish whether the stored grain pests crawl to the attraction area due to the influence of the attractant or due to their own large activity range, thus enabling a more accurate determination of whether the stored grain pests are attracted by the attractant, facilitating the study of the directional behavior of different types of stored grain pests.

[0056] In this invention, the trapping zones 3 are distributed on both sides of the observation zone 2. As needed, a trapping agent can be placed on one side and a control agent on the other side to conduct a control experiment, thereby determining the effectiveness of the trapping agent and whether the behavior of stored grain pests is affected by the trapping agent. Different trapping agents can also be placed on both sides to conduct a comparative experiment, thereby evaluating the effectiveness of different trapping agents and selecting the most effective control method.

[0057] In this invention, the observation area 2 is set in correspondence with the inoculation hole 18 on the box cover 17, which can directly introduce stored grain pests into the observation area 2, making it convenient to place stored grain pests during the experiment. At the same time, it can avoid the introduction of stored grain pests to one side of the trapping area 3, which would affect the accuracy of the experiment.

[0058] In this invention, the observation area 2 and each trapping area 3 are provided with at least one discharge port. More preferably, the discharge port is located at the bottom of the observation area 2 and the bottom of the trapping area 3, so as to facilitate the discharge of the trapping material after the experiment and to count the number of stored grain pests in the trapping material.

[0059] As one possible implementation, no second partition is set in the trapping area, and the trapping area is directly used as grain storage space.

[0060] As one possible implementation, each of the trapping zones 3 is provided with at least one second partition plate 402, and the second partition plates 402 in the two trapping zones 3 are arranged symmetrically on the left and right. The second partition plate 402 divides the interior of each trapping zone 3 into multiple grain storage spaces; each grain storage space is provided with a discharge port.

[0061] In this invention, the trapping area 3 is divided into multiple grain storage spaces because grain-storing pests crawl a certain distance to reach the attractant. This long crawling distance can influence their directional behavior. Therefore, by dividing the trapping area 3 into multiple grain storage spaces, the attractant can be placed in one or more of these spaces, thus adjusting the distance from the attractant to the observation area 2 and the number of attractants. This allows for the investigation of the effective trapping distance for grain-storing pests during control or comparative experiments, providing a reference for the practical use and deployment of the attractant.

[0062] In this invention, each grain storage space is provided with a discharge port, more preferably the discharge port is provided at the bottom of the grain storage space, so as to facilitate the discharge of the attractant after the experiment and to count the number of grain pests in the attractant.

[0063] As one possible implementation, a discharge box 6 is provided at each discharge port. The discharge box 6 includes a collection hopper 10 and a sealing plug 12. The collection hopper 10 has a conical structure that is larger at the top and smaller at the bottom. The bottom of the collection hopper 10 has a discharge port 11. The sealing plug 12 is used to seal the discharge port 11.

[0064] In this invention, a discharge box 6 is provided at each discharge port, and the collection hopper 10 of the discharge box 6 is set into a conical structure with a larger top and a smaller bottom. This facilitates the release of unattracted grain pests or grain placed in the reaction chamber 1 to simulate the actual grain warehouse environment, as well as the attractants and attracted grain pests in the attraction area 3, without leaving any residue. This allows for accurate counting of the number of grain pests in the attraction area 3, thereby ensuring the accuracy of the experimental data.

[0065] In this invention, the sealing plug 12 can block the discharge port 11 by means of threads or interference fit, which can effectively prevent stored grain pests or attractants from escaping or being lost from the discharge port 11, thereby ensuring the accuracy of the experiment. It is also easy to operate, and the sealing plug 12 can be removed when statistics are needed.

[0066] As one possible implementation, a humidity regulating box 5 is also provided in the trapping area 3. The humidity regulating box 5 includes a box body 13, which is a hollow box body. The top of the box body 13 has several humidity regulating holes 22 that are smaller than the particle size of the attractant and the body width of the stored grain pests. The side wall of the box body 13 is provided with hooks 14, and the side wall of the box body 16 is provided with a card plate 15 corresponding to the hooks. The hooks 14 and the card plate 15 are detachably connected.

[0067] In this invention, the humidity control box 5 can roughly adjust the humidity in the collection area 3. Depending on the dryness of the test environment, saturated or unsaturated salt water can be placed in the humidity control box 5 to roughly adjust the humidity in the collection area 3.

[0068] In this invention, the temperature and humidity control device in the prior art can also be used in the trapping area to control the temperature and humidity inside the box; or the temperature and humidity control device in the prior art can be used while setting the humidity box 5. This invention has no special limitation on this.

[0069] In this invention, the humidity regulating box 5 can be detachably connected to the box body 16 via hooks 14 and a card plate 15, and is easy to disassemble, making it convenient to replace or add salt water in the box body 13. Furthermore, when counting the number of stored grain pests, the box body 13 can be removed, and the stored grain pests and attractants attached to the surface of the box body 13 can be removed to prevent stored grain pests from sticking to the surface of the box body 13 and being unable to be discharged from the outlet 11, thus affecting the accuracy of the test.

[0070] In this invention, a humidity regulating hole 22 is provided on the box body 13, so that the humidity regulating box 5 can communicate with the collection area 3, and can roughly regulate the humidity in the collection area 3 during the test.

[0071] As one possible implementation, the inner wall of the housing 16 is provided with a plurality of connecting grooves 9, and a first partition plate 401 or a second partition plate 402 is movably disposed in each connecting groove 9, or a portion of the connecting grooves 9 are movably disposed with a first partition plate 401 or a second partition plate 402; the plurality of connecting grooves 9 are arranged at equal intervals.

[0072] In this invention, only two first partition plates 401 can be set to divide the reaction chamber 1 into an observation area 2 and two trapping areas 3. At this time, each trapping area 3 is a grain storage space. Different traps can be placed in the grain storage spaces on both sides to study the trapping effect of different traps on grain storage pests.

[0073] In this invention, while setting two first partition plates 401 to divide the reaction chamber 1 into an observation area 2 and two trapping areas 3, at least one second partition plate 402 is set in the connecting groove 9 in each trapping area 3. At this time, each trapping area 3 is divided into at least two grain storage spaces. Different attractants can be placed in the grain storage spaces on both sides, so as to simultaneously study the trapping effect of different attractants on grain storage pests and the effective trapping distance of the attractants on grain storage pests.

[0074] In this invention, while setting two first partition plates 401 to divide the reaction chamber 1 into an observation area 2 and two trapping areas 3, second partition plates 402 are set in all the connecting grooves 9 in the trapping areas 3. At this time, attractants are placed into a grain storage space in one trapping area 3, and the same amount of attractants are placed into the grain storage space at an asymmetrical position in the other trapping area 3, and the top surface of the attractants is flat. By placing the same attractants in the grain storage spaces of the trapping areas 3 on both sides, and only one grain storage space in each trapping area 3 will place attractants, the distance between the grain storage space where the attractants are placed and the observation area 2 is different on both sides, so as to study the influence of long-distance crawling on the directional behavior of grain storage pests more directly.

[0075] In this invention, the multiple connecting grooves 9 are arranged at equal intervals, which can ensure the uniform distribution of the partition plates, facilitate standardized experimental setup, and improve the comparability of experimental results.

[0076] As one possible implementation, the first partition plate 401 includes a partition frame 7 and a partition mesh 8, with the partition mesh 8 fixedly disposed in the middle of the partition frame 7; the second partition plate 402 has the same structure as the first partition plate 401.

[0077] In this invention, the external partition frame 7 of the partition net 8 can better support the partition net 8 and provide sufficient strength for the partition net 8. At the same time, the partition frame 7 is movably set in the connecting groove 9, which can better support the first partition plate 401 and effectively prevent the partition net 8 from deforming and failing when filling with attractant. Furthermore, the first partition plate 401 and the connecting groove 9 are detachably connected. When counting the number of stored grain pests, after the attractant is discharged from the discharge box, the first partition plate 401 can also be removed to prevent stored grain pests from sticking to the partition plate and not being discharged from the discharge port 11, which would prevent them from being counted and affect the accuracy of the test.

[0078] As one possible implementation, the aperture of the separating mesh 8 is greater than or equal to the body width of the stored grain pests and smaller than the particle size of the attractant.

[0079] In this invention, the limitation of the aperture of the separating net 8 ensures that the separating net 8 does not obstruct the crawling of stored grain pests, but it can block the attractant, allowing the stored grain pests to enter the attractant area 3 from the observation area 2. This facilitates experiments on the directional behavior of stored grain pests towards different attractants. Therefore, the stored grain pests can also move freely within the attractant area 3. While conducting experiments on the directional behavior of stored grain pests towards different attractants, it is also convenient to conduct experiments to explore the influence of long-distance crawling on the directional behavior of stored grain pests. The same device can be used to study multiple experiments affecting the directional behavior of stored grain pests.

[0080] In this invention, the mesh count of the dividing net 8 can also be selected according to the type or size of the attractant and the stored grain pests, so that a suitable dividing net 8 can be selected according to the specific needs of the experiment to ensure the accuracy of the experiment. For example, for smaller stored grain pests or small attractants, a dividing net 8 with a denser mesh count can be selected to prevent stored grain pests from passing through or attractants from passing through the dividing net 8.

[0081] In addition, as an alternative implementation, the material of the separator 7 can also be transparent acrylic material, which is convenient for observation; it can be clearly determined whether there are any unremoved grain pests or attractants in the connection groove between the separator 7 and the connection groove 9. If not, the next test can be carried out; if so, the separator 7 can be removed to remove all the grain pests or attractants, thereby improving the accuracy of the test.

[0082] As one possible implementation, two rows of guide rails 21 are arranged parallel to each other on the top of the box body 16, and the box cover 17 is slidably connected to the box body 16 through the guide rails 21;

[0083] Alternatively, one end of the lid 17 may be hinged to the body 16, and the other end may be snapped to the body 16 or directly cover the surface of the body 16; or the lid 17 may directly cover the surface of the body 16.

[0084] The reaction chamber 1 also includes a cover 19 for sealing the inoculation hole 18, the cover 19 being adapted to the inoculation hole 18 and detachably connected.

[0085] In this invention, the box body 16 and the box cover 17 are designed to facilitate the disassembly and fixing of the box cover and the box body 16, thereby making it easier to put the attractant into the attractant area; and by setting the sealing cover 19, the inoculation hole 18 can be sealed after the stored grain pests are inoculated, preventing the stored grain pests in the observation area 2 from escaping, and also reducing the influence of external factors on the test results.

[0086] In addition, as an alternative implementation, the reaction chamber 1 may also include a support device fixedly installed on the outside of the chamber body 16. Specifically, the support device may be configured as support feet 20, which are located at the four top corners of the bottom of the chamber body 16. The support feet 20 can lift the chamber body 16 off the ground. When the discharge box 6 is located at the bottom of the reaction chamber 1, it is convenient to properly install and remove the sealing plug 12 at the bottom of the discharge box 6, and it is also convenient to release the attractants and stored grain pests inside the reaction chamber 1.

[0087] As one possible implementation, the bottom of the box 16 is provided with an insert plate 23 that can be inserted from the outside. During the test, the bottom of the box 16 is separated from the inlet of the hopper 10. When it is necessary to discharge the grain, the insert plate 23 is pulled out and the sealing plug 12 is removed to discharge the grain for statistics.

[0088] During the experiment, the bottom of the chamber 16 was separated from the inlet of the hopper 10 to prevent the conical slope of the hopper 10 from affecting the movement of stored pests inside the reaction chamber 1, thereby further improving the accuracy of the experimental data.

[0089] This invention also relates to a method for studying the directional behavior of stored grain pests, comprising the following steps:

[0090] S1: Determine the position of the first partition plate 401 according to the predetermined movement distance of the stored grain pests, and use the first partition plate 401 to divide the reaction box 1 into the observation area 2 and the trapping area 3.

[0091] S2: Design the location and quantity of grain storage space according to experimental needs;

[0092] No second partition 402 is installed in the trapping area 3; the trapping area 3 is directly used as grain storage space.

[0093] Alternatively, at least one second partition 402 may be provided in each trapping area 3 to divide each trapping area 3 into at least multiple grain storage spaces.

[0094] S3: Place attractants into one or more grain storage spaces in one side of the attractant area 3, and place equal amounts of different attractants into grain storage spaces at symmetrical positions in the other side of the attractant area 3, or place equal amounts of the same attractants into grain storage spaces at asymmetrical positions in the other side of the attractant area 3, with the top surface of the attractants being flat.

[0095] When the same amount of attractant is placed into the grain storage space at an asymmetrical position in the other attractant area 3, the effective attracting distance of the attractant to stored grain pests can be analyzed by changing the distance between the grain storage space where the attractant is placed and the observation area 2, and by conducting multiple experiments.

[0096] S4: Place reaction chamber 1 in a dark environment and let it stand still. Inoculate 30-120 stored grain pests into observation area 2 and control the temperature and relative humidity during the experiment.

[0097] S5: After an interval of 2h to 8h, the attractants in each grain storage space are discharged from the discharge port 11, the number of grain storage pests is counted, and the directional data of the grain storage pests are recorded.

[0098] In this invention, each experiment is performed three or more independent replicates, and the stored grain pests that were inoculated once are not reused after one experiment. The final calculation results are taken as the average value, which improves the accuracy of the experiment.

[0099] As one possible implementation, step S3 may further include placing a humidification box 5 filled with saline solution inside the reaction chamber 1.

[0100] To further illustrate the present invention, the technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0101] In the following application examples:

[0102] The response rate refers to the ratio of the total number of stored grain pests in the storage space where the attractant is placed to the total number of stored grain pests inoculated; the attraction rate refers to the ratio of the total number of stored grain pests attracted by each attractant to the total number of stored grain pests; the relative attraction rate refers to the ratio of the difference in the number of stored grain pests attracted by different attractants to the total number of stored grain pests, which can more clearly reflect the directional behavior of stored grain pests towards a certain attractant.

[0103] Application Example 1

[0104] In this embodiment, the reaction chamber 1 is 120cm long, 10cm wide, and 15cm high. The selected grain pest is the rusty red flour beetle, whose adult body length is 1.70-2.34mm and body width is 1.0mm. Considering the body length and width of the rusty red flour beetle and the size of common grain seeds, the mesh size of the separator 8 is set to 10 meshes with a pore size of 2mm. The wheat grains selected for the experiment have a width of 4.4mm-4.6mm and a length of 6.5mm-7.5mm. When other types of grain pests are selected, the mesh size and pore size of the separator 8 should be adjusted accordingly.

[0105] In this embodiment, eleven connecting slots 9 are evenly spaced on the inner wall of the box 16. The distance between two adjacent connecting slots 9 is 10cm. A discharge port is provided between each adjacent connecting slot 9. A discharge port is also provided between the connecting slots 9 at both ends and the box 19. The predetermined movement distance of the rusty red flat grain beetle is 20cm. Based on the predetermined movement distance of the rusty red flat grain beetle, the length of the observation area is determined to be 20cm. The fifth and seventh connecting slots 9 from left to right in the reaction box 1 are movably equipped with a first partition plate 401. The first partition plate 401 divides the reaction box 1 into an observation area 2 and two trapping areas 3. The two trapping areas 3 are of equal size and each has a length of 50cm.

[0106] In this embodiment, each of the trapping areas 3 is provided with a second partition plate 402 to divide each trapping area 3 into two grain storage spaces. One grain storage space is 20cm long and the other grain storage space is 30cm long. The grain storage space closer to the observation area 2 is 20cm long. The second partition plates 402 in the two trapping areas 3 are arranged symmetrically on the left and right.

[0107] In this embodiment, a humidification box 5 filled with saturated saline solution in the left trapping area 3 is placed in the middle of the third and fourth connecting slots 9 from left to right in the reaction chamber 1, and a humidification box 5 is also provided at a symmetrical position in the right trapping area 3.

[0108] In this embodiment, normal wheat is placed in a grain storage space with a length of 30cm in one trapping area 3, and an equal amount of moldy wheat is placed in a grain storage space at a symmetrical position in the other trapping area 3. No wheat is placed in the observation area. The height of the wheat is not less than 75% of the internal height of the box 16 to facilitate the movement of the rusty red flat grain beetle within the trap. The normal wheat serves as the control group, and the moldy wheat serves as the experimental group.

[0109] In this embodiment, when the test temperature is maintained at (28±1)℃ and the relative humidity is maintained at 65%±5%, the existing temperature and humidity control components can be used to achieve stability and humidity control. Therefore, they will not be described in detail in this embodiment. Fifty healthy rusty red flat grain beetles are inoculated into the observation area 2, and then the box cover 17 is closed to allow the rusty red flat grain beetles to move freely without the influence of the external environment.

[0110] Two hours later, the sealing plug 12 was opened, and normal wheat and moldy wheat were discharged from the discharge port 11 respectively. The number of rusty red flatbread beetles inside was counted. After opening the box cover 17 to confirm that there were no remaining rusty red flatbread beetles in the reaction box 1, the relative attraction rate was calculated. Each experiment was conducted in three independent replicates, and after each 50 inoculated heads were tested once, they were not reused. The final calculation result was the average value.

[0111] The experimental data are shown below:

[0112] Table 1. Number of insects attracted in the observation area when no wheat was placed and the experiment lasted for 2 hours.

[0113]

[0114] When no wheat was placed in the observation area and the experiment lasted for 2 hours, the response rate of *Strombus rusticus* to moldy and normal wheat was 92.67% ± 4.06%; the attraction rate of moldy wheat to *Strombus rusticus* was 53.33% ± 3.33%, while the attraction rate of normal wheat to *Strombus rusticus* was 39.33% ± 4.06%; the relative attraction rate of moldy wheat to *Strombus rusticus* compared to normal wheat was 14.00% ± 7.21%.

[0115] Comparison of experimental data revealed that placing moldy wheat in trapping area 3 attracted more insects and had a higher attraction rate than placing normal wheat, with a significant difference between the two. This demonstrates that the rusty flour beetle exhibits a more pronounced tactic behavior towards moldy wheat compared to normal wheat.

[0116] In this embodiment, the response rate refers to the ratio of the total number of rusty red flour beetles induced by moldy wheat and normal wheat to the total number of rusty red flour beetles inoculated; the attraction rate refers to the ratio of the number of rusty red flour beetles induced by moldy wheat or normal wheat to the total number of rusty red flour beetles inoculated; the attraction rate is the ratio of the number of rusty red flour beetles induced by moldy wheat minus the number of rusty red flour beetles induced by normal wheat to the total number of rusty red flour beetles inoculated, which can more clearly reflect the directional behavior of rusty red flour beetles towards moldy wheat. h refers to hours, and 2h means 2 hours.

[0117] Example 2

[0118] The technical solution in this embodiment is the same as that in embodiment 1, except that:

[0119] While placing normal wheat and moldy wheat into the two trapping areas 3 respectively, normal wheat of the same height was placed into the observation area 2. When inoculating with the rusty red flour beetle, the rusty red flour beetle was inoculated onto the normal wheat in the observation area 2, and all other conditions were the same.

[0120] During the statistical analysis, the grain in trapping zone 3 was emptied at 2h, 4h, and 8h respectively, and the number of rusty red grain beetles inside was counted, and the relative attraction rate was calculated.

[0121] In this embodiment, the 2h, 4h, and 8h tests were conducted under the same conditions in different groups. The number of rust-red flat grain beetles was counted only in each group at one designated time, without repeated counting, to prevent disturbing the rust-red flat grain beetles and affecting the test results.

[0122] The experimental data are shown below:

[0123] Table 2 shows the number of insects attracted when normal wheat was placed in the observation area and the experiment lasted for 2 hours.

[0124]

[0125] Table 3. Number of insects attracted when normal wheat was placed in the observation area and the experiment lasted for 4 hours.

[0126]

[0127] Table 4. Number of insects attracted when normal wheat was placed in the observation area and the experiment lasted for 8 hours.

[0128]

[0129] The experimental results show that even when normal wheat of the same height is placed in observation area 2, when the experimental time is 2h, 4h, and 8h, the number of rusty red flour beetles attracted by moldy wheat in trapping area 3 is greater than that attracted by normal wheat, and there is a significant difference between the two. This proves that rusty red flour beetles have a more obvious directional behavior towards moldy wheat than normal wheat, and the number of rusty red flour beetles attracted by both moldy wheat and normal wheat increases with the extension of experimental time.

[0130] When wheat was placed in the observation area and the experiment lasted for 2 hours, the response rate of *Strombus rusticus* to moldy wheat and normal wheat was 11.33% ± 1.15%; the attraction rate of moldy wheat to *Strombus rusticus* was 9.33% ± 1.76%, while the attraction rate of normal wheat to *Strombus rusticus* was 2.00% ± 1.15%; the relative attraction rate of moldy wheat to *Strombus rusticus* compared to normal wheat was 7.33% ± 0.67%.

[0131] When wheat was placed in the observation area and the experiment lasted for 4 hours, the response rate of *Strombus rusticus* to moldy wheat and normal wheat was 23.33% ± 0.67%; the attraction rate of moldy wheat to *Strombus rusticus* was 18.67% ± 4.67%, and the attraction rate of normal wheat to *Strombus rusticus* was 4.67% ± 0.67%; the relative attraction rate of moldy wheat to *Strombus rusticus* compared with normal wheat was 14.00% ± 4.62%.

[0132] When wheat was placed in the observation area and the experiment lasted for 8 hours, the response rate of *Strombus rusticus* to moldy wheat and normal wheat was 36.67% ± 1.15%; the attraction rate of moldy wheat to *Strombus rusticus* was 26.67% ± 5.21%, while the attraction rate of normal wheat to *Strombus rusticus* was 10.00% ± 1.15%; the relative attraction rate of moldy wheat to *Strombus rusticus* compared to normal wheat was 16.67% ± 4.81%.

[0133] Experimental data show that as the experimental time increases, the response rate, attraction rate, and relative attraction rate of each group all increase, and the relative attraction rate at 4 hours is significantly higher than that at 2 hours. As the experimental time increases, the attraction rate of moldy wheat to the rusty red flour beetle is greater than that of normal wheat, and there is a significant difference between the two. This proves that the tactic behavior of the rusty red flour beetle towards moldy wheat is obvious compared to normal wheat, and it increases with the extension of experimental time.

[0134] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A device for studying the tactic behavior of stored grain pests, comprising a reaction chamber (1), wherein the reaction chamber (1) is a hollow box, characterized in that, The reaction chamber (1) includes a chamber body (16) and a chamber cover (17). The chamber cover (17) is located on the upper part of the chamber body (16), and an inoculation hole (18) is provided in the middle of the chamber cover (17). The reaction chamber (1) is provided with two first partition plates (401), which divide the interior of the reaction chamber (1) into an observation area (2) and two trapping areas (3). The two trapping areas (3) are respectively distributed on both sides of the observation area (2), and the inoculation hole (18) is located above the observation area (2). An observation area (2) and two trapping areas (3) are each equipped with a discharge port; Each of the trapping areas (3) is provided with at least one second partition plate (402). The second partition plates (402) in the two trapping areas (3) are arranged symmetrically on the left and right. The second partition plate (402) divides the interior of each trapping area (3) into multiple grain storage spaces. Each of the aforementioned grain storage spaces is equipped with a discharge port; The first partition plate (401) includes a partition frame (7) and a partition net (8), wherein the partition net (8) is fixedly disposed in the middle of the partition frame (7); the second partition plate (402) has the same structure as the first partition plate (401); the aperture of the partition net (8) is greater than or equal to the body width of the stored grain pest and smaller than the particle size of the attractant; The location and size of the observation area (2) and the trapping area (3) can be adjusted as needed by the first partition (401); the location of the first partition (401) is determined according to the predetermined movement distance of the stored grain pests; Each discharge port is provided with a discharge box (6), the discharge box (6) includes a collection hopper (10) and a sealing plug (12). The collection hopper (10) is a conical structure with a larger top and a smaller bottom. The bottom of the collection hopper (10) has a discharge port (11). The sealing plug (12) is used to block the discharge port (11).

2. The storage pest directional behavior research device according to claim 1, characterized in that: The trapping area (3) is also equipped with a humidity regulating box (5). The humidity regulating box (5) includes a box body (13). The box body (13) is a hollow box body. The top of the box body (13) is provided with several humidity regulating holes (22) smaller than the particle size of the attractant and the body width of the stored grain pests. The side wall of the box body (13) is provided with hooks (14). The side wall of the box body (16) is provided with a card plate (15) corresponding to the hooks. The hooks (14) and the card plate (15) are detachably connected.

3. The storage pest directional behavior research device according to claim 1, characterized in that: The inner wall of the box (16) is provided with a plurality of connecting grooves (9), and each connecting groove (9) is provided with a first partition plate (401) or a second partition plate (402), or a portion of the connecting grooves (9) are provided with a first partition plate (401) or a second partition plate (402); the plurality of connecting grooves (9) are arranged at equal intervals.

4. The storage pest directional behavior research device according to claim 1, characterized in that: The top of the box (16) is provided with two parallel rows of guide rails (21), and the box cover (17) is slidably connected to the box (16) through the guide rails (21); Alternatively, one end of the lid (17) is hinged to the box body (16), and the other end is snapped to the box body (16) or directly covers the surface of the box body (16); Alternatively, the lid (17) can be placed directly over the surface of the box (16); The reaction chamber (1) also includes a cover (19) for sealing the inoculation hole (18), the cover (19) being adapted to and detachably connected to the inoculation hole (18).

5. The method for studying the directional behavior of stored grain pests using the storage pest directional behavior research device according to any one of claims 1-4, characterized in that, Includes the following steps: Step 1: Determine the position of the first partition plate (401) according to the predetermined movement distance of the stored grain pests, and use the first partition plate (401) to divide the reaction box (1) into an observation area (2) and a trapping area (3). Step 2: Design the location and quantity of grain storage space according to experimental needs; Each trapping area (3) is provided with at least one second partition (402) to divide each trapping area (3) into at least a plurality of grain storage spaces; Step 3: Place attractants into one or more grain storage spaces in one side of the attractant area (3), and place different amounts of attractants into the grain storage spaces at symmetrical positions in the other side of the attractant area (3), or place the same amount of attractants into the grain storage spaces at asymmetrical positions in the other side of the attractant area (3), and the top surface of the attractants is flat. Step 4: Place the reaction chamber (1) in a dark environment and let it stand. Inoculate the observation area (2) with a fixed number of stored grain pests and control the temperature and relative humidity during the experiment. Step 5: After an interval of 2h to 8h, the attractants in each grain storage space are discharged from the discharge port (11), the number of grain storage pests is counted, and the trend data of grain storage pests are recorded.

6. The method for studying the directional behavior of stored grain pests according to claim 5, characterized in that: Before step 3, the process also includes placing a humidification box (5) filled with saline solution inside the reaction chamber (1).

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