Processing method of Arisaema cum Bile and its differentiation method from Arisaema heterophyllum

Abstract

This invention discloses a method for processing Arisaema cum Bile and its identification method with Arisaema heterophyllum, comprising the following steps: taking Arisaema heterophyllum and 2-6 times its total mass of bovine bile, 0.3-0.7 times its total mass of citric acid aqueous solution, 0.1-0.5 times its total mass of calcium lactate aqueous solution, and 0.1-0.3 times its total mass of spirulina powder, adding them to a bovine gallbladder, tying it tightly, and hanging it for fermentation, thus obtaining Arisaema cum Bile. This invention achieves an increase in bile acid content by adding citric acid aqueous solution, calcium lactate aqueous solution, and spirulina.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine processing technology. More specifically, this invention relates to a method for processing Arisaema cum Bile and a method for differentiating it from Arisaema heterophyllum. Background Technology

[0002] Arisaema cum Bile is a traditional Chinese medicine listed in the Chinese Pharmacopoeia and subject to national drug approval number management. It is mainly processed from Arisaema cum Bile and bovine, mutton, or pig bile, or made by fermenting Arisaema cum Bile in bovine, mutton, or pig bile. It is usually in the form of cubes or cylindrical blocks, with a brownish-yellow, grayish-brown, or brownish-black color, a relatively hard texture, a slightly fishy odor, and a bitter taste. The main function of Arisaema cum Bile is to clear heat and resolve phlegm. Its bitter and cooling properties allow it to dry dampness and resolve phlegm, while its cooling properties clear heat. It can be used to treat symptoms such as cough with phlegm and fire, dizziness due to headache, phlegm obstruction due to stroke, senile mental fatigue, postpartum palpitations, and insomnia due to phlegm and fire. Current methods for preparing Arisaema cum Bile generally use fermentation, but this process reduces the bile acid content, affecting the quality of the product. Summary of the Invention

[0003] One object of the present invention is to solve at least the above-mentioned problems and to provide at least the advantages that will be described later.

[0004] Another objective of this invention is to provide a method for processing Arisaema cum Bile, which increases the bile acid content by adding citric acid aqueous solution, calcium lactate aqueous solution, and spirulina.

[0005] To achieve these objectives and other advantages of the present invention, a method for processing Arisaema cum Bile is provided, comprising the following steps:

[0006] Take Arisaema heterophyllum and 2-6 times its total mass of ox bile, 0.3-0.7 times its total mass of citric acid aqueous solution, 0.1-0.5 times its total mass of calcium lactate aqueous solution, and 0.1-0.3 times its total mass of spirulina powder, add them to the ox gallbladder, tie it tightly, and hang it to ferment, thus obtaining Arisaema heterophyllum in gallbladder.

[0007] Preferably, before adding Arisaema heterophyllum and citric acid aqueous solution to the bovine gallbladder, the citric acid aqueous solution is divided into three equal portions for later use. The Arisaema heterophyllum is first pulverized and passed through a 10-mesh sieve to obtain the first pulverized material. One portion of the citric acid aqueous solution is mixed with the first pulverized material and subjected to auxiliary ultrasonic treatment under conditions of 60W ultrasonic power, 20kHz frequency, 45℃ temperature, and 20min ultrasonic time. The first pulverized material is then pulverized and passed through a 40-mesh sieve to obtain the second pulverized material. One portion of the citric acid aqueous solution is mixed with the first pulverized material and subjected to auxiliary ultrasonic treatment under conditions of 200W ultrasonic power, 40kHz frequency, 45℃ temperature, and 25min ultrasonic time. The second pulverized material is then pulverized and passed through a 100-mesh sieve to obtain the third pulverized material. The remaining portion of the citric acid aqueous solution is mixed with the third pulverized material to obtain pretreated Arisaema heterophyllum, which is then added to the bovine gallbladder.

[0008] Preferably, the pretreated Arisaema is mixed with an aqueous solution of calcium lactate, placed at -6°C and cooled to -18°C, frozen at -18°C for 2 hours, heated to -6°C and kept at -6°C for 3 hours, then removed and exposed to room temperature and nitrogen atmosphere for 15 minutes. This process is repeated three times, wherein the temperature change rate during the heating and cooling processes is 2°C / h.

[0009] Preferably, the citric acid aqueous solution has a mass fraction of 25%.

[0010] Preferably, the mass fraction of the calcium lactate aqueous solution is 8%.

[0011] This invention also provides a method for differentiating between Arisaema cum Bile and Arisaema heterophyllum, comprising the following steps:

[0012] Step 1: Sample preparation: Add 5g of the sample to be tested to 50mL of 95% ethanol and soak for 12h. Then, treat it under the conditions of 200W power and 40kHz frequency for 60min. Filter and remove the solvent, add 20mL of water to dissolve, and then extract with petroleum ether or ethyl acetate. Take the organic phase and remove the solvent. Add 5mL of methanol to dissolve to obtain the sample to be tested. Arisaema heterophyllum and Arisaema cum bile are treated by the above method to obtain the Arisaema heterophyllum sample and Arisaema cum bile sample to be tested.

[0013] Step 2: Take 3 μL each of the Arisaema heterophyllum and Arisaema cum Bile test samples and spot them on the same silica gel thin-layer plate for development. Remove and air dry, develop the color with 10% sulfuric acid ethanol solution, and examine under a 365 nm ultraviolet lamp. By comparing and analyzing the spots on the same silica gel thin-layer plate, the Arisaema heterophyllum and Arisaema cum Bile test samples can be distinguished.

[0014] Preferably, the developing solvent is a chloroform-acetone system or a petroleum ether-ethyl acetate system.

[0015] Preferably, the volume ratio of chloroform to acetone is 8:1.

[0016] Preferably, the volume ratio of petroleum ether to ethyl acetate is 1.5:1.

[0017] The present invention has at least the following beneficial effects:

[0018] This invention increases the bile acid content by adding citric acid aqueous solution, calcium lactate aqueous solution, and spirulina. By adding citric acid aqueous solution, calcium lactate aqueous solution, and spirulina, and by improving the addition method of citric acid aqueous solution and calcium lactate aqueous solution, this invention can effectively increase the bile acid content in Arisaema cum Bile and enhance the efficacy of Arisaema cum Bile.

[0019] Other advantages, objectives and features of the present invention will become apparent in part from the following description, and in part from those skilled in the art through study and practice of the invention. Attached Figure Description

[0020] Figure 1 This is a thin-layer chromatogram for distinguishing between Arisaema heterophyllum and Arisaema cum Bile, according to one embodiment of the present invention;

[0021] Figure 2 This is a thin-layer chromatogram for distinguishing between Arisaema heterophyllum and Arisaema cum bile, according to one embodiment of the present invention. Detailed Implementation

[0022] The present invention will be further described in detail below with reference to embodiments, so that those skilled in the art can implement it based on the description.

[0023] <Example 1>

[0024] The method for processing Arisaema cum Bile includes the following steps:

[0025] Take Arisaema heterophyllum and twice the total mass of Arisaema heterophyllum in ox bile, 0.3 times the amount of citric acid aqueous solution, 0.1 times the amount of calcium lactate aqueous solution, and 0.1 times the amount of spirulina powder, mix them, add them to the ox gallbladder, tie it tightly, and hang it to ferment, thus obtaining Arisaema heterophyllum in ox bile.

[0026] Before adding Arisaema heterophyllum and citric acid aqueous solution to the bovine gallbladder, the citric acid aqueous solution was divided into three equal portions for later use. Arisaema heterophyllum was first pulverized and passed through a 10-mesh sieve to obtain the first pulverized material. One portion of the citric acid aqueous solution was mixed with the first pulverized material and subjected to auxiliary ultrasonic treatment under conditions of 60W ultrasonic power, 20kHz frequency, 45℃ temperature, and 20min ultrasonic time. The first pulverized material was then pulverized and passed through a 40-mesh sieve to obtain the second pulverized material. One portion of the citric acid aqueous solution was mixed with the first pulverized material and subjected to auxiliary ultrasonic treatment under conditions of 200W ultrasonic power, 40kHz frequency, 45℃ temperature, and 25min ultrasonic time. The second pulverized material was then pulverized and passed through a 100-mesh sieve to obtain the third pulverized material. The remaining portion of the citric acid aqueous solution was mixed with the third pulverized material to obtain pretreated Arisaema heterophyllum, which was then added to the bovine gallbladder.

[0027] Pretreated Arisaema was mixed with calcium lactate aqueous solution, placed at -6℃ and cooled from -6℃ to -18℃, frozen at -18℃ for 2 hours, heated from -18℃ to -6℃ and kept at -6℃ for 3 hours, then removed and exposed to room temperature and nitrogen environment for 15 minutes. The operation was repeated three times, with the temperature change rate of the heating and cooling processes being 2℃ / h.

[0028] The citric acid aqueous solution has a mass fraction of 25%.

[0029] <Example 2>

[0030] The method for processing Arisaema cum Bile includes the following steps:

[0031] Take Arisaema heterophyllum and 6 times the total mass of Arisaema heterophyllum in ox bile, 0.7 times the amount of citric acid aqueous solution, 0.5 times the amount of calcium lactate aqueous solution, and 0.3 times the amount of spirulina powder, mix them, add them to the ox gallbladder, tie it tightly, and hang it to ferment, thus obtaining Arisaema heterophyllum in ox bile.

[0032] Before adding Arisaema heterophyllum and citric acid aqueous solution to the bovine gallbladder, the citric acid aqueous solution was divided into three equal portions for later use. Arisaema heterophyllum was first pulverized and passed through a 10-mesh sieve to obtain the first pulverized material. One portion of the citric acid aqueous solution was mixed with the first pulverized material and subjected to auxiliary ultrasonic treatment under conditions of 60W ultrasonic power, 20kHz frequency, 45℃ temperature, and 20min ultrasonic time. The first pulverized material was then pulverized and passed through a 40-mesh sieve to obtain the second pulverized material. One portion of the citric acid aqueous solution was mixed with the first pulverized material and subjected to auxiliary ultrasonic treatment under conditions of 200W ultrasonic power, 40kHz frequency, 45℃ temperature, and 25min ultrasonic time. The second pulverized material was then pulverized and passed through a 100-mesh sieve to obtain the third pulverized material. The remaining portion of the citric acid aqueous solution was mixed with the third pulverized material to obtain pretreated Arisaema heterophyllum, which was then added to the bovine gallbladder.

[0033] Pretreated Arisaema was mixed with calcium lactate aqueous solution, placed at -6℃ and cooled from -6℃ to -18℃, frozen at -18℃ for 2 hours, heated from -18℃ to -6℃ and kept at -6℃ for 3 hours, then removed and exposed to room temperature and nitrogen environment for 15 minutes. The operation was repeated three times, with the temperature change rate of the heating and cooling processes being 2℃ / h.

[0034] The citric acid aqueous solution has a mass fraction of 25%.

[0035] <Example 3>

[0036] The method for processing Arisaema cum Bile includes the following steps:

[0037] Take Arisaema heterophyllum and 4 times the total mass of Arisaema heterophyllum in ox bile, 0.5 times the amount of citric acid aqueous solution, 0.3 times the amount of calcium lactate aqueous solution, and 0.2 times the amount of spirulina powder, mix them, add them to the ox gallbladder, tie it tightly, and hang it to ferment, thus obtaining Arisaema heterophyllum in ox bile.

[0038] Before adding Arisaema heterophyllum and citric acid aqueous solution to the bovine gallbladder, the citric acid aqueous solution was divided into three equal portions for later use. Arisaema heterophyllum was first pulverized and passed through a 10-mesh sieve to obtain the first pulverized material. One portion of the citric acid aqueous solution was mixed with the first pulverized material and subjected to auxiliary ultrasonic treatment under conditions of 60W ultrasonic power, 20kHz frequency, 45℃ temperature, and 20min ultrasonic time. The first pulverized material was then pulverized and passed through a 40-mesh sieve to obtain the second pulverized material. One portion of the citric acid aqueous solution was mixed with the first pulverized material and subjected to auxiliary ultrasonic treatment under conditions of 200W ultrasonic power, 40kHz frequency, 45℃ temperature, and 25min ultrasonic time. The second pulverized material was then pulverized and passed through a 100-mesh sieve to obtain the third pulverized material. The remaining portion of the citric acid aqueous solution was mixed with the third pulverized material to obtain pretreated Arisaema heterophyllum, which was then added to the bovine gallbladder.

[0039] Pretreated Arisaema was mixed with calcium lactate aqueous solution, placed at -6℃ and cooled from -6℃ to -18℃, frozen at -18℃ for 2 hours, heated from -18℃ to -6℃ and kept at -6℃ for 3 hours, then removed and exposed to room temperature and nitrogen environment for 15 minutes. The operation was repeated three times, with the temperature change rate of the heating and cooling processes being 2℃ / h.

[0040] The citric acid aqueous solution has a mass fraction of 25%.

[0041] <Example 4>

[0042] The method for processing Arisaema cum Bile includes the following steps:

[0043] Take Arisaema heterophyllum and 4 times the total mass of Arisaema heterophyllum in ox bile, 0.5 times the amount of citric acid aqueous solution, 0.3 times the amount of calcium lactate aqueous solution, and 0.2 times the amount of spirulina powder, mix them, add them to the ox gallbladder, tie it tightly, and hang it to ferment, thus obtaining Arisaema heterophyllum in ox bile.

[0044] Before adding Arisaema heterophyllum and citric acid aqueous solution to the bovine gallbladder, the citric acid aqueous solution was divided into three equal portions for later use. Arisaema heterophyllum was first pulverized and passed through a 10-mesh sieve to obtain the first pulverized material. One portion of the citric acid aqueous solution was mixed with the first pulverized material and subjected to auxiliary ultrasonic treatment under conditions of 60W ultrasonic power, 20kHz frequency, 45℃ temperature, and 20min ultrasonic time. The first pulverized material was then pulverized and passed through a 40-mesh sieve to obtain the second pulverized material. One portion of the citric acid aqueous solution was mixed with the first pulverized material and subjected to auxiliary ultrasonic treatment under conditions of 200W ultrasonic power, 40kHz frequency, 45℃ temperature, and 25min ultrasonic time. The second pulverized material was then pulverized and passed through a 100-mesh sieve to obtain the third pulverized material. The remaining portion of the citric acid aqueous solution was mixed with the third pulverized material to obtain pretreated Arisaema heterophyllum, which was then added to the bovine gallbladder.

[0045] Pretreated Arisaema was mixed with calcium lactate aqueous solution, placed at -6℃ and cooled from -6℃ to -18℃, frozen at -18℃ for 2 hours, heated from -18℃ to -6℃ and kept at -6℃ for 3 hours, then removed and exposed to room temperature and nitrogen environment for 15 minutes. The operation was repeated three times, with the temperature change rate of the heating and cooling processes being 2℃ / h.

[0046] The citric acid aqueous solution has a mass fraction of 25%.

[0047] A method for differentiating between Arisaema cum Bile and Arisaema heterophyllum, characterized by comprising the following steps:

[0048] Step 1: Sample Preparation: Add 5g of the sample to be tested to 50mL of 95% ethanol and soak for 12 hours. Then, treat it under conditions of 200W power and 40kHz frequency for 60 minutes. Filter and remove the solvent, dissolve in 20mL of water, and extract with petroleum ether. Take the organic phase, remove the solvent, and dissolve in 5mL of methanol to obtain the sample to be tested. Arisaema heterophyllum and Arisaema cum Bile are treated using the above method to obtain the Arisaema heterophyllum sample and Arisaema cum Bile sample (add 5g of Arisaema heterophyllum to 50mL of 95% ethanol, soak for 12 hours, and then treat under conditions of 200W power and 40kHz frequency for 60 minutes). The sample was treated at 40 kHz for 60 min, filtered to remove the solvent, dissolved in 20 mL of water, and then extracted with petroleum ether or ethyl acetate. The organic phase was collected, the solvent was removed, and 5 mL of methanol was added to dissolve the sample to obtain the Arisaema cum Bile test sample. 5 g of Arisaema cum Bile was added to 50 mL of 95% ethanol and soaked for 12 h. Then it was treated at 200 W and 40 kHz for 60 min, filtered to remove the solvent, dissolved in 20 mL of water, and then extracted with petroleum ether or ethyl acetate. The organic phase was collected, the solvent was removed, and 5 mL of methanol was added to dissolve the sample to obtain the Arisaema cum Bile test sample.

[0049] Step 2: Take 3 μL each of the Arisaema heterophyllum and Arisaema cum Bile test samples and spot them on the same silica gel thin-layer plate. Develop the plate using chloroform-acetone as the developing solvent. Remove the plate and let it dry. Develop the color with 10% sulfuric acid ethanol solution and examine it under a 365 nm ultraviolet lamp. By comparing and analyzing the spots on the same silica gel thin-layer plate, the Arisaema heterophyllum and Arisaema cum Bile test samples can be distinguished.

[0050] The volume ratio of chloroform to acetone is 8:1.

[0051] <Example 5>

[0052] The method for processing Arisaema cum Bile includes the following steps:

[0053] Take Arisaema heterophyllum and 4 times the total mass of Arisaema heterophyllum in ox bile, 0.5 times the amount of citric acid aqueous solution, 0.3 times the amount of calcium lactate aqueous solution, and 0.2 times the amount of spirulina powder, mix them, add them to the ox gallbladder, tie it tightly, and hang it to ferment, thus obtaining Arisaema heterophyllum in ox bile.

[0054] Before adding Arisaema heterophyllum and citric acid aqueous solution to the bovine gallbladder, the citric acid aqueous solution was divided into three equal portions for later use. Arisaema heterophyllum was first pulverized and passed through a 10-mesh sieve to obtain the first pulverized material. One portion of the citric acid aqueous solution was mixed with the first pulverized material and subjected to auxiliary ultrasonic treatment under conditions of 60W ultrasonic power, 20kHz frequency, 45℃ temperature, and 20min ultrasonic time. The first pulverized material was then pulverized and passed through a 40-mesh sieve to obtain the second pulverized material. One portion of the citric acid aqueous solution was mixed with the first pulverized material and subjected to auxiliary ultrasonic treatment under conditions of 200W ultrasonic power, 40kHz frequency, 45℃ temperature, and 25min ultrasonic time. The second pulverized material was then pulverized and passed through a 100-mesh sieve to obtain the third pulverized material. The remaining portion of the citric acid aqueous solution was mixed with the third pulverized material to obtain pretreated Arisaema heterophyllum, which was then added to the bovine gallbladder.

[0055] Pretreated Arisaema was mixed with calcium lactate aqueous solution, placed at -6℃ and cooled from -6℃ to -18℃, frozen at -18℃ for 2 hours, heated from -18℃ to -6℃ and kept at -6℃ for 3 hours, then removed and exposed to room temperature and nitrogen environment for 15 minutes. The operation was repeated three times, with the temperature change rate of the heating and cooling processes being 2℃ / h.

[0056] The citric acid aqueous solution has a mass fraction of 25%.

[0057] A method for differentiating between Arisaema cum Bile and Arisaema heterophyllum, characterized by comprising the following steps:

[0058] Step 1: Sample Preparation: Add 5g of the sample to be tested to 50mL of 95% ethanol and soak for 12 hours. Then, treat it at 200W power and 40kHz frequency for 60 minutes. Filter and remove the solvent, dissolve in 20mL of water, and extract with ethyl acetate. Take the organic phase, remove the solvent, and dissolve in 5mL of methanol to obtain the sample to be tested. Both Arisaema heterophyllum and Arisaema cum Bile are treated using the above method to obtain the Arisaema heterophyllum sample and Arisaema cum Bile sample (add 5g of Arisaema heterophyllum to 50mL of 95% ethanol, soak for 12 hours, and then treat at 200W power and 40kHz frequency for 60 minutes). The sample was treated at a frequency of 40 kHz for 60 min, filtered to remove the solvent, dissolved in 20 mL of water, and then extracted with petroleum ether or ethyl acetate. The organic phase was collected, the solvent was removed, and 5 mL of methanol was added to dissolve the sample to obtain the Arisaema cum Bile test sample. 5 g of Arisaema cum Bile was added to 50 mL of 95% ethanol and soaked for 12 h. Then it was treated at a power of 200 W and a frequency of 40 kHz for 60 min, filtered to remove the solvent, dissolved in 20 mL of water, and then extracted with petroleum ether or ethyl acetate. The organic phase was collected, the solvent was removed, and 5 mL of methanol was added to dissolve the sample to obtain the Arisaema cum Bile test sample.

[0059] Step 2: Take 3 μL each of the Arisaema heterophyllum and Arisaema cum Bile test samples and spot them on the same silica gel thin-layer plate. Develop the plate with petroleum ether-ethyl acetate as the developing solvent. Remove the plate and let it dry. Develop the color with 10% sulfuric acid ethanol solution and examine it under a 365 nm ultraviolet lamp. By comparing and analyzing the spots on the same silica gel thin-layer plate, the Arisaema heterophyllum and Arisaema cum Bile test samples can be distinguished.

[0060] The volume ratio of petroleum ether to ethyl acetate is 1.5:1.

[0061] <Comparative Example 1>

[0062] The method of Example 3 was used to prepare Arisaema cum Bile, except that: Arisaema cum Bile and 2-6 times the total mass of Arisaema cum Bile were added to the gallbladder of a cow, tied tightly and hung to ferment, thus obtaining Arisaema cum Bile.

[0063] <Comparative Example 2>

[0064] The method of Example 3 was used to prepare Arisaema cum Bile, except that: Arisaema cum Bile and 2-6 times the total mass of Arisaema cum Bile, 0.3-0.7 times the amount of citric acid aqueous solution, and 0.1-0.5 times the amount of calcium lactate aqueous solution were added to the gallbladder of a cow, tied tightly, and hung for fermentation to obtain Arisaema cum Bile.

[0065] <Comparative Example 3>

[0066] Arisaema cum Bile was prepared using the method described in Example 3, except that: Arisaema cum Bile was pulverized and passed through a 100-mesh sieve to obtain Arisaema cum Bile powder, and citric acid aqueous solution was mixed with Arisaema cum Bile powder in one step to obtain pretreated Arisaema cum Bile.

[0067] <Comparative Example 4>

[0068] Arisaema cum Bile was prepared using the method described in Example 3, except that: pretreated Arisaema cum Bile, calcium lactate aqueous solution, and spirulina powder were mixed and added to bovine gallbladder for fermentation to obtain Arisaema cum Bile.

[0069] <Results Analysis>

[0070] 1. Bile acid content

[0071] The Arisaema cum Bile obtained in Example 3 and Comparative Examples 1-4 were prepared into medicinal slices, and the bile acid content was determined. The results are shown in Table 1.

[0072] Table 1 shows the bile acid content.

[0073] Example 3 0.051 Comparative Example 1 0.029 Comparative Example 2 0.042 Comparative Example 3 0.043 Comparative Example 4 0.046

[0074] Comparative analysis of Example 3 and Comparative Examples 1-4 shows that the present invention can effectively increase the content of cholic acid in Arisaema cum Bile and enhance the efficacy of Arisaema cum Bile by adding citric acid water, calcium lactate aqueous solution, spirulina and improving the addition method of citric acid water and calcium lactate aqueous solution.

[0075] 2. Thin-layer chromatogram

[0076] The thin-layer chromatography of Arisaema heterophyllum and Arisaema cum Bile was performed using the identification method described in Example 4. Figure 1 As shown ( Figure 1 (1-3 spots indicate Arisaema heterophyllum, 4-6 spots indicate Arisaema cum Bile). The thin-layer chromatography of Arisaema heterophyllum and Arisaema cum Bile was performed using the identification method described in Example 5. Figure 2 As shown ( Figure 2 (1-3 points indicate Arisaema heterophyllum, 4-6 points indicate Arisaema cum bile)

[0077] Results analysis: Figure 1 As can be seen, in points 1-3, Arisaema heterophyllum shows fluorescent spots A and D of the same color, while in points 4-6, Arisaema cum bile adds fluorescent spots B and C. That is, Arisaema heterophyllum and Arisaema cum bile can be distinguished by the newly added spots.

[0078] Figure 2 As can be seen, Arisaema heterophyllum and Arisaema cum Bile show the same fluorescent spots B and C. The fluorescence of spot B in Arisaema cum Bile is weakened, and new fluorescent spots A, D and E are added in Arisaema cum Bile. That is, Arisaema heterophyllum and Arisaema cum Bile can be distinguished by the new spots.

[0079] Although embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and embodiments. They can be applied to various fields suitable for the present invention. For those skilled in the art, other modifications can be easily made. Therefore, without departing from the general concept defined by the claims and their equivalents, the present invention is not limited to the specific details and embodiments shown and described herein.

Claims

1. A method for processing Arisaema cum Bile, characterized in that, Includes the following steps: Take Arisaema heterophyllum and 2-6 times its total mass of ox bile, 0.3-0.7 times its total mass of citric acid aqueous solution, 0.1-0.5 times its total mass of calcium lactate aqueous solution, and 0.1-0.3 times its total mass of spirulina powder, add them to the ox gallbladder, tie it tightly, and hang it to ferment, thus obtaining Arisaema heterophyllum in gallbladder.

2. The method for processing Arisaema cum Bile as described in claim 1, characterized in that, Before adding Arisaema heterophyllum and citric acid aqueous solution to the bovine gallbladder, the citric acid aqueous solution was divided into three equal portions for later use. Arisaema heterophyllum was first pulverized and passed through a 10-mesh sieve to obtain the first pulverized material. One portion of the citric acid aqueous solution was mixed with the first pulverized material and subjected to auxiliary ultrasonic treatment under conditions of 60W ultrasonic power, 20kHz frequency, 45℃ temperature, and 20min ultrasonic time. The first pulverized material was then pulverized and passed through a 40-mesh sieve to obtain the second pulverized material. One portion of the citric acid aqueous solution was mixed with the second pulverized material and subjected to auxiliary ultrasonic treatment under conditions of 200W ultrasonic power, 40kHz frequency, 45℃ temperature, and 25min ultrasonic time. The second pulverized material was then pulverized and passed through a 100-mesh sieve to obtain the third pulverized material. The remaining portion of the citric acid aqueous solution was mixed with the third pulverized material to obtain pretreated Arisaema heterophyllum, which was then added to the bovine gallbladder.

3. The method for processing Arisaema cum Bile as described in claim 2, characterized in that, Pretreated Arisaema was mixed with calcium lactate aqueous solution, placed at -6℃ and cooled from -6℃ to -18℃, frozen at -18℃ for 2 hours, heated from -18℃ to -6℃ and kept at -6℃ for 3 hours, then removed and exposed to room temperature and nitrogen environment for 15 minutes. The operation was repeated three times, with the temperature change rate of the heating and cooling processes being 2℃ / h.

4. The method for processing Arisaema cum Bile as described in claim 1, characterized in that, The mass fraction of the citric acid aqueous solution is 25%.

5. The method for processing Arisaema cum Bile as described in claim 1, characterized in that, The mass fraction of the calcium lactate aqueous solution is 8%.

6. The method for distinguishing between Arisaema cum Bile and Arisaema heterophyllum prepared according to any one of claims 1-5, characterized in that, Includes the following steps: Step 1: Sample preparation: Add 5g of the sample to be tested to 50mL of 95% ethanol and soak for 12h. Then, treat it under the conditions of 200W power and 40kHz frequency for 60min. Filter and remove the solvent, add 20mL of water to dissolve, and then extract with petroleum ether or ethyl acetate. Take the organic phase and remove the solvent. Add 5mL of methanol to dissolve to obtain the sample to be tested. Arisaema heterophyllum and Arisaema cum bile are treated by the above method to obtain the Arisaema heterophyllum sample and Arisaema cum bile sample to be tested. Step 2: Take 3 μL each of the Arisaema heterophyllum and Arisaema cum Bile test samples and spot them on the same silica gel thin-layer plate for development. Remove and air dry, develop the color with 10% sulfuric acid ethanol solution, and examine under a 365 nm ultraviolet lamp. By comparing and analyzing the spots on the same silica gel thin-layer plate, the Arisaema heterophyllum and Arisaema cum Bile test samples can be distinguished.

7. The method for distinguishing between Arisaema cum Bile and Arisaema heterophyllum as described in claim 6, characterized in that, The developing solvent is either chloroform-acetone or petroleum ether-ethyl acetate.

8. The method for distinguishing between Arisaema cum Bile and Arisaema heterophyllum as described in claim 7, characterized in that, The volume ratio of chloroform to acetone is 8:

1.

9. The method for distinguishing between Arisaema cum Bile and Arisaema heterophyllum as described in claim 7, characterized in that, The volume ratio of petroleum ether to ethyl acetate is 1.5:1.

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