A carbonyl reductase mutant and its application in the synthesis of 4-AA chiral intermediates

Abstract

This invention provides a carbonyl reductase mutant and (2) S ,3 R An enzymatically catalyzed asymmetric synthesis method for methyl 2-benzoylaminomethyl-3-hydroxybutyrate. This invention uses methyl 2-benzoylaminomethyl-3-carbonylbutyrate as a substrate and a carbonyl reductase mutant as a catalyst to synthesize (2-)-methyl 2-benzoylaminomethyl-3-hydroxybutyrate under mild conditions. S ,3 R 2-benzoylaminomethyl-3-hydroxybutyrate methyl ester. The carbonyl reductase mutant provided by this invention can completely convert 25% of 2-benzoylaminomethyl-3-hydroxybutyrate methyl ester to synthesize optically pure (2-benzoylaminomethyl-3-hydroxybutyrate methyl ester within 5.5 h. S ,3 R Methyl 2-benzoylaminomethyl-3-hydroxybutyrate was synthesized with a space-time yield of up to 45.4 g / L / h. This method requires no addition of other enzyme systems or expensive coenzymes, leaves no substrate residue, is easy to purify, and achieves a synthesis yield of up to 95%. It has advantages such as high space-time yield, high atom economy, easy purification, and environmental friendliness.

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