一种赤芍活性成分检测方法

The vortex-assisted matrix solid-phase dispersion microextraction method using natural eutectic solvents solves the problems of low efficiency and environmental pollution of traditional extraction methods, and achieves efficient and environmentally friendly detection of active ingredients in Paeonia lactiflora.

CN118688336BActive Publication Date: 2026-07-17TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
Filing Date
2024-06-20
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

In existing technologies, traditional extraction methods such as ultrasonic extraction and Soxhlet extraction are cumbersome and inefficient, use large amounts of organic solvents which are unfriendly to the environment and health, and are difficult to meet the needs of large-scale production or rapid analysis.

Method used

A vortex-assisted matrix solid-phase dispersion microextraction method based on a natural eutectic solvent was adopted, using the natural eutectic solvent Bet-Ma as the eluent, combined with UPLC for chromatographic analysis, to optimize the extraction and quantitative analysis of active ingredients in Paeonia lactiflora.

Benefits of technology

It enables efficient, environmentally friendly, and rapid detection of active ingredients in Paeonia lactiflora, reduces the use of organic solvents, and is suitable for quality evaluation of Paeonia lactiflora and quantitative analysis of various active ingredients.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN118688336B_ABST
    Figure CN118688336B_ABST
Patent Text Reader

Abstract

本发明公开了一种赤芍活性成分检测方法,将待提取药材与吸附剂的按照质量比例为1:3加入;所述吸附剂为石墨,将混合物研磨后研磨时间为2min;使用天然低共熔溶剂作为洗脱剂,按照每80mg混合物加入1000μL体积的洗脱剂,洗脱剂的含水量为50%,涡旋时间为3分钟后取上清液过滤作为供试品溶液用于UPLC分析。同时测定了赤芍中5种化合物,包括单萜类化合物、多元酚类化合物以及酚酸类化合物。本发明所建立的方法具有提取效率高、操作简单、环境友好的特点,因此该方法为中药绿色质量评价体系的构建提供参考。
Need to check novelty before this filing date? Find Prior Art