Dendrobium compound extract with regulating gastrointestinal function, preparation method and application thereof

Abstract

The present application belongs to the technical field of health food, and relates to a dendrobium compound extract with the function of regulating gastrointestinal function, and a preparation method and application thereof. In view of the technical problems that in the prior art, when the gastrointestinal function is not suitable, using chemical drugs may cause obvious side effects, and a single drug-food homologous plant has poor effect, the present application provides the dendrobium compound extract with the function of regulating gastrointestinal function, and the preparation steps are as follows: 5-15 parts of dried dendrobium officinale, 5-20 parts of yam, 2-10 parts of poria cocos and 2-10 parts of white horsebean are powdered and sieved through a 40-80 mesh sieve; under the condition of water bath at 70-100 DEG C, 5-15 times the volume of water is added, and uniform stirring is performed for 3-6 hours; and then the mixture is filtered through gauze. The extract can be applied to the preparation of health food, is used for improving alcoholic or acetic acid gastric mucosa injury, can inhibit the decrease of superoxide dismutase content or the increase of malondialdehyde content caused by ethanol, and can inhibit the decrease of motilin or gastrin content caused by senna leaf.

Description

Technical Field

[0001] This invention belongs to the field of health food technology, specifically relating to a compound extract of Dendrobium that regulates gastrointestinal function, its preparation method, and its application. Background Technology

[0002] The gastric mucosa is a protective layer for the stomach, ensuring its normal function. When the stomach is overstimulated or overworked, its dynamic balance is disrupted, leading to the production of gastric acid that gradually damages the stomach wall and causes gastric mucosal damage. Alcohol consumption is a significant factor contributing to hemorrhagic gastric mucosal injury. Ethanol is absorbed into the bloodstream through the gastrointestinal tract and comes into direct contact with the gastric mucosa, altering its metabolism and function. Anhydrous ethanol is highly corrosive, dissolving fatty compounds and disrupting the normal physiological environment, leading to damage to the gastric mucosal barrier, increased cell membrane permeability, and resulting in gastric mucosal damage such as edema, congestion, erosion, bleeding, and even ulcers.

[0003] Commonly used clinical medications for relieving acute and chronic gastric mucosal damage include omeprazole, cimetidine, lansoprazole, and bismuth potassium citrate. Their basic function is to inhibit gastric acid secretion, thereby protecting the gastric mucosa from gastric acid irritation and promoting damage repair. Domperidone tablets are commonly used to treat dyspepsia caused by delayed gastric emptying, gastroesophageal reflux, and esophagitis. However, oral omeprazole, in addition to inhibiting gastric acid secretion from gastric mucosal cells, can also lead to bacterial overgrowth in the stomach. This bacteria act on multiple parts of the body through the bloodstream, potentially damaging glandular function, especially the respiratory mucosa. The European Gastroenterological Association has stated that approximately 40% of patients who have taken omeprazole for more than one year have developed vacuolar proton pumps in osteoclasts, resulting in excessive osteoblast activity, affecting bone resorption, increasing bone fragility, and raising the probability of fractures.

[0004] Compared to chemical drugs, traditional Chinese medicine (TCM) compound prescriptions offer greater safety and gentleness in treating acute gastric injury. Combining medicinal and edible plants for the prevention and treatment of acute and chronic gastric mucosal damage or functional dyspepsia is a feasible approach for developing stomach-nourishing functional products. Therefore, developing a set of TCM formulas with proportions of medicinal and edible plants that have significant repair effects on acute gastric mucosal damage and functional dyspepsia has important application value. *Dendrobium officinale* is a perennial herb belonging to the genus *Dendrobium* of the Orchidaceae family, distributed in Fujian, Zhejiang, Anhui, and other regions. Traditional Chinese medicine considers *Dendrobium officinale* to nourish the stomach and promote the production of body fluids, nourish yin and clear heat, and it is listed as the "first of the nine great Chinese herbs." The *Shennong Bencao Jing* records that *Dendrobium officinale* is a commonly used plant for treating stomach pain and upper abdominal distension. Polysaccharides are the main active ingredients in *Dendrobium officinale*, and numerous studies have shown that *Dendrobium officinale* polysaccharides have significant antioxidant, immune-enhancing, anti-tumor, blood sugar-lowering, and gastrointestinal-regulating effects. For example, Chinese patent application publication number CN112851830A, application date March 17, 2021, entitled "A Method for Extracting Polysaccharides from Dendrobium officinale and the Application of the Prepared Products", discloses the following steps: pulverizing dried Dendrobium officinale and weighing an appropriate amount; fixing the material-liquid ratio, extraction temperature, extraction time, and ultrasonic power, and extracting in pulse mode; after sufficient extraction, concentrating the polysaccharide solution and removing impurities using anhydrous ethanol. This method involves a complicated preparation process, and the components in Dendrobium officinale are complex. In addition, previous studies have used an ethanol-induced acute gastric mucosal injury model to evaluate the protective effect of Dendrobium officinale polysaccharides on the gastric mucosa. Compared with the model group, the Dendrobium officinale group showed less gastric mucosal bleeding upon gross observation. HE staining pathological sections showed local sloughing of the epithelial mucosa, reduced mucosal bleeding area, and decreased number of inflammatory cells in the Dendrobium officinale group. Measurements of SOD or MDA levels in rat gastric tissue showed that Dendrobium officinale polysaccharides mainly protect the gastric mucosa by reducing the release of pro-inflammatory factors, inhibiting the release of oxygen free radicals, and promoting the secretion of epidermal growth factor. Western blot results showed that Dendrobium officinale polysaccharides can inhibit the activation of the ERK signaling pathway, reduce MMP-2 secretion, and decrease MMP-9 expression, thus reducing damage. However, studying only the single active ingredient of polysaccharides cannot demonstrate the overall therapeutic effect of Dendrobium officinale, and existing research indicates that multiple herbal combinations can have synergistic effects, although the specific formulation ratios and efficacy are not yet clear. Summary of the Invention

[0005] 1. The problem to be solved

[0006] In view of the technical problem that the use of chemical drugs may cause obvious side effects when gastrointestinal dysfunction occurs, and that the effect of single medicinal and edible plants is not good, this application provides a compound extract of Dendrobium that can regulate gastrointestinal function and can be used in the preparation of health food.

[0007] 2. Technical Solution

[0008] To achieve the above objectives, the provided technical solution is as follows:

[0009] The Dendrobium compound extract with gastrointestinal function of this application is made from the following medicinal and edible plant raw materials in the following weight ratio: Dendrobium officinale 5-15 parts, Dioscorea opposita 5-20 parts, Poria cocos 2-10 parts, and Dolichos lablab 2-10 parts.

[0010] Furthermore, it is made from the following medicinal and edible plant materials in the following weight ratios: 5 parts Dendrobium officinale, 7 parts Dioscorea opposita, 4 parts Poria cocos, and 4 parts Dolichos lablab.

[0011] A method for preparing a Dendrobium compound extract that regulates gastrointestinal function includes the following steps:

[0012] S1. Take dried Dendrobium officinale, Dioscorea opposita, Poria cocos, and white hyacinth bean, grind them into powder, pass them through a 40-80 mesh sieve, and calculate by weight: Dendrobium officinale 5-15 parts, Dioscorea opposita 5-20 parts, Poria cocos 2-10 parts, and white hyacinth bean 2-10 parts.

[0013] S2. Under water bath conditions of 70-100℃, add 5-15 times the volume of water and stir thoroughly for 3-6 hours;

[0014] S3. Filter the mixture through gauze to obtain the Dendrobium compound extract.

[0015] Furthermore, it includes the following steps:

[0016] S1. Take dried Dendrobium officinale, Dioscorea opposita, Poria cocos and white hyacinth bean, grind them into powder, pass through a 60-mesh sieve, and calculate by weight: Dendrobium officinale 5 parts, Dioscorea opposita 7 parts, Poria cocos 4 parts, white hyacinth bean 4 parts.

[0017] S2. Add 10 times the volume of water and stir thoroughly for 4 hours under 85℃ water bath conditions;

[0018] The Dendrobium compound extract was obtained by filtration through S3.100 mesh gauze.

[0019] Application of Dendrobium compound extract with gastrointestinal function: The Dendrobium compound extract is applied to the preparation of health food.

[0020] Furthermore, the Dendrobium compound extract is applied in the preparation of health food products that improve alcoholic or acetic acid-induced gastric mucosal damage.

[0021] Furthermore, the health food can inhibit the decrease in superoxide dismutase content or the increase in malondialdehyde content caused by ethanol.

[0022] Furthermore, the health food can inhibit the decrease in motilin or gastrin content caused by senna leaves.

[0023] Furthermore, the Dendrobium compound extract can be used directly or in the form of a composition, wherein the composition comprises 0.1 to 99 wt% of the Dendrobium compound extract, with the remainder being a carrier or excipient. The Dendrobium compound extract can be used in the form of powder, granules, tablets, pills, powders, syrups, capsules, or liquid preparations.

[0024] 3. Beneficial effects

[0025] Compared with existing known technologies, the technical solution provided by this invention has the following beneficial effects:

[0026] (1) The Dendrobium compound extract of the present invention, which regulates gastrointestinal function, contains only Dendrobium officinale, Dioscorea opposita, Poria cocos, and Dolichos lablab. It can be used in the preparation of health food to improve alcoholic or acetic acid-induced gastric mucosal damage, inhibit the decrease in superoxide dismutase content or the increase in malondialdehyde content caused by ethanol, and also inhibit the decrease in motilin or gastrin content caused by senna leaves.

[0027] (2) The preparation method of the Dendrobium compound extract with gastrointestinal function of the present invention is simple and easy to implement, and does not require the use of any chemical extractants. Attached Figure Description

[0028] Figure 1 This is a graph showing the trend of rat body weight change in Example 2.

[0029] Figure 2 This is a representative stomach unfolding diagram of a rat in Example 2.

[0030] Figure 3 The damage index of the rat gastric mucosa in Example 2.

[0031] Figure 4 The inhibition rate of gastric mucosal damage in rats in Example 2.

[0032] Figure 5 This is a representative HE-stained pathological section of rat gastric tissue from Example 2.

[0033] Figure 6 The superoxide dismutase content in rat gastric tissue in Example 2.

[0034] Figure 7 The content of malondialdehyde in the rat gastric tissue in Example 2.

[0035] Figure 8 This is a graph showing the trend of rat body weight change in Example 3.

[0036] Figure 9 This is a representative stomach unfolding diagram of a rat in Example 3.

[0037] Figure 10 The damage index of the rat gastric mucosa in Example 3.

[0038] Figure 11 The inhibition rate of gastric mucosal damage in rats in Example 3.

[0039] Figure 12 This is a representative HE-stained pathological section of rat gastric tissue from Example 3.

[0040] Figure 13 This is a graph showing the trend of rat body weight change in Example 4.

[0041] Figure 14 The gastric emptying rate of rats in Example 4 is shown.

[0042] Figure 15 The small intestinal propulsion rate in rats in Example 4.

[0043] Figure 16 The rat serum motilin content is shown in Example 4.

[0044] Figure 17 The value represents the serum gastrin content in rats in Example 4.

[0045] Note: NC represents the normal group, MOD represents the model group, OME represents the positive drug omeprazole group, CMD represents the positive drug cimetidine group, DPD represents the positive drug domperidone group, WE represents the Dendrobium compound extract aqueous extract group (powder extract group), and DOE represents the Dendrobium extract group. Detailed Implementation

[0046] To further understand the content of this invention, a detailed description of the invention will be provided in conjunction with the embodiments and accompanying drawings.

[0047] Glossary

[0048] Superoxide dismutase (SOD);

[0049] Malondialdehyde (MDA);

[0050] Motilin (MTL);

[0051] Gastrin (GAS);

[0052] Hematoxylin-eosin staining, or HE staining for short.

[0053] Example 1

[0054] The Dendrobium compound extract with gastrointestinal function regulation involved in this example is all prepared by the following method and used for subsequent in vivo animal experiment verification.

[0055] The Dendrobium compound extract with gastrointestinal function regulation in this example is calculated in parts by mass: 5 parts of Dendrobium officinale, 7 parts of Chinese yam, 4 parts of Poria cocos, and 4 parts of white hyacinth bean.

[0056] The preparation steps of the Dendrobium compound extract with gastrointestinal function regulation in this example are as follows:

[0057] Use a high-speed pulverizer to pulverize Dendrobium officinale, Chinese yam, Poria cocos, and white hyacinth bean into fine powders, pass through a 60-mesh sieve, take the sieved medicinal powder, add 10 times the volume of distilled water, and fully stir and extract for 4 hours under the water bath condition of 85°C. After the extraction is completed, filter with 100-mesh gauze, and the obtained filtrate is the aqueous extract of the Dendrobium compound extract with gastrointestinal function regulation.

[0058] The preparation steps of the aqueous extract of a single plant with both medicinal and edible homology in this example are as follows:

[0059] Take 20 parts each of Chinese yam, Dendrobium officinale, Poria cocos, and white hyacinth bean powder, add 10 times the volume of distilled water, fully stir and extract for 4 hours under the water bath condition of 85°C. After the extraction is completed, filter with 100-mesh gauze, and the obtained filtrate is the aqueous extract of the single plant with both medicinal and edible homology.

[0060] Example 2

[0061] The following example is the experimental method and experimental result of the protective effect of the above-mentioned Dendrobium compound extract on ethanol-induced acute gastric mucosal injury in rats, which are specifically as follows:

[0062] I. Experimental method

[0063] 1. Experimental animals

[0064] 4-week-old SPF grade male SD rats weighing 200±20g were ordered from Hunan Silaike Jingda Laboratory Animal Co., Ltd. (License No.: SCXK(Xiang)2019-0004); animal feed and bedding were purchased from Hunan Silaike Jingda Laboratory Animal Co., Ltd. (License No.: SCXK(Xiang)2020-0006).

[0065] 2. Establishment of ethanol-induced acute gastric mucosal injury model in rats

[0066] Thirty-two 4-week-old male SD rats (body weight 200±20g) were selected and divided into four groups according to body weight: normal control group (NC), model group (MOD), positive drug group (OME), and Dendrobium officinale compound extract aqueous extract group (WE), with eight rats in each group. After one week of acclimatization, the OME group rats were given omeprazole aqueous solution (20mg / kg) daily according to body weight, the WE group rats were given Dendrobium officinale compound extract aqueous extract (100mg / kg) daily according to body weight, the DOE group rats were given Dendrobium officinale aqueous extract (100mg / kg) daily according to body weight, and the normal and model groups were given physiological saline daily according to body weight. This treatment continued for 14 days by gavage. On day 15, after a 12-hour period of strict fasting with free access to water, all groups except the NC group were gavaged with 1mL of anhydrous ethanol. One hour after gavage with anhydrous ethanol, the rats were euthanized by cervical dislocation, and the gastric tissue was collected.

[0067] 3. Gastric mucosal damage index measurement

[0068] The stomach was cut open along the greater curvature, rinsed with saline, dried with filter paper, and the gastric mucosa was photographed. The total area of ​​the gastric glands and the area of ​​gastric mucosal bleeding were measured using ImageJ image processing software. The gastric mucosal injury index and gastric mucosal injury inhibition rate were calculated according to the following formulas.

[0069] Gastric mucosal injury index / % = (gastric mucosal bleeding area / corresponding total area of ​​gastric glandular tissue) * 100%;

[0070] Gastric mucosal injury inhibition rate / % = [(Gastric mucosal injury index of model group - gastric mucosal injury index of treatment group) / gastric mucosal injury index of model group] * 100%.

[0071] 4. Pathological observation of gastric tissue

[0072] A tissue sample of approximately 10 mm x 2 mm was excised from the most severely damaged area of ​​the mouse gastric mucosa, fixed in 4% paraformaldehyde solution, dehydrated, and embedded in paraffin. The paraffin-embedded tissue block was sectioned, stained with hematoxylin and eosin (HE), and the pathological changes of the gastric tissue were observed under an optical microscope (200x).

[0073] 5. Measurement of oxidative stress levels in gastric tissue

[0074] Gastric tissue was rinsed with physiological saline, blotted dry with filter paper, and weighed. The gastric tissue was mixed with PBS at a volume ratio of 1:4 and then homogenized using a tissue homogenizer to prepare a 20% gastric tissue homogenate. The homogenate was centrifuged at 10000 rpm at 4°C for 10 min, and the supernatant was collected, aliquoted, and stored at -80°C. The total SOD activity and MDA content in the gastric tissue homogenate were determined according to the kit instructions.

[0075] 6. Data Statistics and Analysis

[0076] Experimental data are expressed as mean ± standard deviation (Mean ± SD). Data analysis was performed using GraphPadPrism 8 software, and one-way ANOVA analysis was used to analyze differences between groups. *P < 0.05 indicates a significant difference compared to the model group; **P < 0.01 indicates a highly significant difference compared to the model group; ***P < 0.001 indicates a highly significant difference compared to the model group; ****P < 0.0001 indicates a highly significant difference compared to the model group.

[0077] II. Experimental Results

[0078] 1. Changes in rat body weight

[0079] according to Figure 1 It can be seen that the rats' body weight steadily increased during the gavage period.

[0080] 2. Effect of Dendrobium compound extract aqueous extract on gastric mucosal injury index in rats with acute gastric mucosal injury.

[0081] according to Figure 2 Observation of gastric mucosal morphology revealed that, compared with the normal group, the model group showed numerous hemorrhagic streaks, obvious ulcers, and erosions in the gastric mucosa. The gastric mucosal damage area in the Dendrobium compound extract group was significantly improved compared with the damage area in the model group, with only a few hemorrhagic streaks appearing. Figure 3 Figure 4 The results of the gastric mucosal injury index and gastric mucosal injury inhibition rate showed that the model group had the highest injury index and the most severe injury. Compared with the model group, the WE group significantly reduced the gastric mucosal injury index (P < 0.001).

[0082] 3. Effects of the aqueous extract of Dendrobium compound extract on the gastric histopathology of rats with acute gastric mucosal injury.

[0083] Depend on Figure 5 HE staining images of mouse gastric tissue showed that the normal group had an intact, smooth, flat gastric mucosa with normal morphology, regular and tightly arranged mucosal epithelial cells, and no shedding, necrosis, or inflammatory cell infiltration. The model group mice showed severe damage to the gastric mucosa, exhibiting obvious glandular structural disorder, extensive epithelial cell shedding, and inflammatory cell infiltration. However, pretreatment with the aqueous extract of Dendrobium officinale compound extract could protect the integrity of the gastric glandular structure and alleviate the disordered glandular structure and inflammatory cell infiltration.

[0084] 4. Effects of Dendrobium compound extract aqueous extract on oxidative stress in gastric tissue of rats with acute gastric mucosal injury.

[0085] Depend on Figure 6-7It was found that, compared with the normal group, the SOD content in the model group was significantly decreased, and the MDA level was significantly increased (P < 0.01). Compared with the model group, intervention with the aqueous extract of Dendrobium officinale compound extract significantly increased the SOD content and significantly decreased the MDA content. Therefore, the aqueous extract of Dendrobium officinale compound extract has a significant effect on balancing the oxidative level of rat gastric tissue.

[0086] Example 3

[0087] The following examples illustrate the experimental methods and results regarding the protective effect of the aqueous extract of the above-mentioned Dendrobium compound extract against acetic acid-induced chronic gastric mucosal injury in rats:

[0088] I. Experimental Methods

[0089] 1. Laboratory animals

[0090] Same as Example 2.

[0091] 2. Establishment of a rat model of chronic gastric mucosal injury induced by glacial acetic acid

[0092] SD rats were rested for 7 days, and the model was established on day 8. Before surgery, rats were fasted for 24 hours but allowed free access to water, and then anesthetized by intraperitoneal injection of 1.5 mL of 10% chloral hydrate. After anesthesia, a 2-3 cm incision was made below the xiphoid process of the stomach, and the intact stomach was excised. A glass tube with an inner diameter of 8 mm and a length of approximately 30 mm was placed vertically in the anterior part of the stomach, and 200 μL of 80% glacial acetic acid was added to the center of the tube. After reacting for 1 minute, the acetic acid was removed using a Pasteur pipette. The tube was then rinsed several times with physiological saline, and any remaining acetic acid was removed with a cotton ball soaked in physiological saline. The surgical incision was sutured, and the rats were fed normally postoperatively. In the normal group, after opening the abdominal cavity, physiological saline was used instead of glacial acetic acid for related treatments, followed by suturing. Rats in good growth condition were selected and regrouped. The rats were administered the medication by gavage for 14 days, and on day 24, they were sacrificed, the stomach tissue was cut open, and photographs were taken.

[0093] 3. Gastric mucosal damage index measurement

[0094] Same as Example 2.

[0095] 4. Pathological observation of gastric tissue

[0096] Same as Example 2.

[0097] 5. Data Statistics and Analysis

[0098] Same as Example 2.

[0099] II. Experimental Results

[0100] 1. Changes in rat body weight

[0101] according to Figure 8It can be seen that the rats' body weight steadily increased during the gavage period.

[0102] 2. Effect of Dendrobium officinale compound extract aqueous extract on gastric mucosal injury index in rats with chronic gastric mucosal injury.

[0103] according to Figure 9 Morphological observation of the gastric mucosa revealed that, compared to the normal group, the model group showed significant damage to the gastric tissue, with typical round or oval crater-like gastric ulcers observed on the gastric mucosa. The aqueous extract of Dendrobium officinale compound extract significantly improved the area of ​​gastric mucosal damage compared to the model group, and the gastric ulcers were alleviated. Figure 10 Figure 11 The results of the gastric mucosal injury index and gastric mucosal injury inhibition rate showed that the model group had the highest injury index and the most severe injury. Compared with the model group, the WE group reduced the gastric mucosal injury index.

[0104] 3. Effects of the aqueous extract of Dendrobium compound extract on the gastric histopathology of rats with chronic gastric mucosal injury.

[0105] Depend on Figure 12 HE staining images of mouse gastric tissue showed that the normal group had an intact, smooth, flat gastric mucosa with normal morphology, regular and tightly arranged mucosal epithelial cells, and no signs of sloughing, necrosis, or inflammatory cell infiltration. The model group mice showed severe damage to the gastric mucosa, with obvious glandular structural disorder and extensive epithelial cell shedding. However, pretreatment with the aqueous extract of Dendrobium officinale compound extract could protect the integrity of the gastric glandular structure and alleviate the disordered glandular structure and epithelial cell loss.

[0106] Example 4

[0107] The following examples illustrate the experimental methods and results regarding the protective effect of the aqueous extract of the above-mentioned Dendrobium compound extract against senna leaf intervention and tail-clamping method-induced functional dyspepsia in rats:

[0108] I. Experimental Methods

[0109] 1. Laboratory animals

[0110] Same as Example 2.

[0111] 2. Establishment of a rat model of chronic gastric mucosal injury induced by glacial acetic acid

[0112] SD rats were rested for 7 days and then fasted every other day. On the fasting days, they were administered senna leaf decoction (2g / 100mL) by gavage, and their tails were clamped to induce fighting. The model was established for 14 days. From the 8th day of model establishment, experimental intervention was carried out, with different groups administered gavage for 14 days. Successful model establishment was marked by: alternating constipation and diarrhea in the model group, and a decrease in body weight and food intake. Positive drug group: domperidone suspension (to promote gastrointestinal motility) 3mg / kg. On day 21, after gavage, the rats were fasted for 24 hours. On day 22, 1mL of carbon powder semi-solid paste was administered by gavage. The rats were sacrificed 20 minutes later, and the gastric emptying rate, small intestinal propulsion rate, organ index, gastric tissue, and serum were collected.

[0113] 3. Determination of gastric emptying rate and small intestinal propulsion rate

[0114] Gastric emptying rate determination: The stomach was ligated at the pylorus and cardia, and the total gastric mass (i.e., the weight of gastric contents + gastric tissue) was weighed. The stomach was cut open along the greater curvature, the gastric contents were washed away, and the net gastric mass (i.e., the weight of gastric tissue) was measured after drying. Gastric emptying rate (%) = [total gastric mass (mg) - net mass (mg)] / mass of carbon ink semi-solid paste (mg) * 100;

[0115] Small intestinal propulsion rate determination: Take a whole segment of small intestine from the pylorus at the top and the ileocecal junction at the bottom, gently peel it off, and lay it straight on white paper without traction. Measure the distance from the pylorus to the front edge of the black semi-solid paste and the total length of the small intestine. Small intestinal propulsion rate (%) = distance moved by the black semi-solid paste (mm) / total length of small intestine (mm) * 100.

[0116] 4. Measurement of serum gastrointestinal hormones

[0117] After blood was collected from the eyeballs, the mixture was allowed to stand for 1 hour, then centrifuged at 3500 r / min for 20 min (4℃). The supernatant was collected and the contents of MTL and GAS were determined according to the kit instructions.

[0118] 5. Data Statistics and Analysis

[0119] Same as Example 2.

[0120] II. Experimental Results

[0121] 1. Changes in rat body weight

[0122] according to Figure 13 It can be seen that, except for the normal group of rats, the body weight of the other groups of rats tended to be stable during the modeling period, and the body weight steadily increased during the intervention period after the modeling was completed.

[0123] 2. Effects of Dendrobium compound extract aqueous extract on gastrointestinal motility in rats with functional dyspepsia

[0124] according to Figure 14-15It can be seen that, compared with the normal group, the gastric emptying rate of rats in the model group was significantly reduced and the small intestinal propulsion rate showed a decreasing trend. After intervention with the aqueous extract of Dendrobium compound extract, both the gastric emptying rate and the small intestinal propulsion rate were significantly increased, which was consistent with the effect of the positive drug domperidone, and can effectively improve gastrointestinal motility.

[0125] 3. Effects of Dendrobium officinale compound extract aqueous extract on serum gastrointestinal hormone levels in rats with functional dyspepsia

[0126] according to Figure 16-17 It can be seen that, compared with the normal group, the motilin content of rats in the model group was significantly reduced, and the gastrin content showed a decreasing trend. After intervention with the aqueous extract of Dendrobium compound extract, both motilin and gastrin showed an increasing trend, indicating that the aqueous extract of Dendrobium compound extract has a regulatory effect on serum gastrointestinal hormone levels.

[0127] Example 5

[0128] The Dendrobium compound extract for regulating gastrointestinal function and its preparation method in this embodiment are basically the same as those in Examples 2-4, except that:

[0129] S1. Take dried Dendrobium officinale, Dioscorea opposita, Poria cocos and white hyacinth bean, grind them into powder, pass through a 40-mesh sieve, and calculate by weight: 5 parts Dendrobium officinale, 5 parts Dioscorea opposita, 2 parts Poria cocos and 2 parts white hyacinth bean.

[0130] S2. Add 5 times the volume of water and stir thoroughly for 3 hours under a 70℃ water bath.

[0131] S3. Filter the mixture through gauze to obtain the Dendrobium compound extract.

[0132] The Dendrobium compound extract prepared in this embodiment, which regulates gastrointestinal function, has the same effect as in Example 1.

[0133] Example 6

[0134] The Dendrobium compound extract for regulating gastrointestinal function and its preparation method in this embodiment are basically the same as those in Examples 2-4, except that...

[0135] S1. Take dried Dendrobium officinale, Dioscorea opposita, Poria cocos, and white hyacinth bean, grind them into powder, pass them through an 80-mesh sieve, and calculate by weight: Dendrobium officinale 15 parts, Dioscorea opposita 20 parts, Poria cocos 10 parts, and white hyacinth bean 10 parts.

[0136] S2. Add 15 times the volume of water and stir thoroughly for 6 hours under a 100℃ water bath.

[0137] S3. Filter the mixture through gauze to obtain the Dendrobium compound extract.

[0138] The Dendrobium compound extract prepared in this embodiment, which regulates gastrointestinal function, has the same effect as in Example 1.

[0139] Comparative Example 1

[0140] The following comparative examples illustrate the experimental methods and results regarding the protective effect of the above-mentioned single medicinal and edible plant aqueous extracts against ethanol-induced acute gastric mucosal injury in rats:

[0141] I. Experimental Methods

[0142] Same as Example 2.

[0143] II. Experimental Results

[0144] 1. Effects of aqueous extracts of single medicinal and edible plants on the gastric mucosal injury index in rats with acute gastric mucosal injury.

[0145] Based on observation of gastric mucosal morphology, compared with the normal group of rats, the model group showed numerous hemorrhagic streaks, obvious ulcers, and erosions in the gastric mucosa. The single-plant-derived aqueous extract group showed some improvement in the area of ​​gastric mucosal damage compared to the model group, but the number of hemorrhagic streaks was greater than that in the Dendrobium officinale compound extract group (Example 2). The results for the gastric mucosal damage index and the gastric mucosal damage inhibition rate were similar to the results for the area of ​​damage.

[0146] 2. Effects of aqueous extracts from single medicinal and edible plants on gastric histopathology in rats with acute gastric mucosal injury.

[0147] HE staining images of mouse gastric tissue showed that the normal group had an intact, smooth, flat gastric mucosa with normal morphology, regular and tightly arranged epithelial cells, and no shedding, necrosis, or inflammatory cell infiltration. The model group mice showed severe damage to the gastric mucosa, exhibiting obvious glandular structural disorder, extensive epithelial cell shedding, and inflammatory cell infiltration. Pretreatment with a single aqueous extract of a medicinal and edible plant showed some efficacy in alleviating gastric glandular structural disorder, but its effect on improving inflammatory cell infiltration was not as good as that of the Dendrobium officinale compound extract.

[0148] Chemical drugs have significant side effects, and the efficacy of combined formulations is uncertain. Therefore, this application provides a Dendrobium officinale compound extract prepared from a combination of Dendrobium officinale, Dioscorea opposita, Poria cocos, and Dolichos lablab, which has the effect of relieving acute and chronic gastric mucosal damage and functional dyspepsia. In vivo animal experiments have verified the efficacy of the aqueous extract of the Dendrobium officinale compound extract. Studies have shown that the aqueous extract of the Dendrobium officinale compound extract has a good effect in relieving ethanol-induced gastric mucosal damage in rats, a good effect in relieving acetic acid-induced gastric ulcers in rats, and a good effect in relieving senna leaf or tail-clamping method-induced functional dyspepsia in rats, providing an important theoretical basis for developing safer and milder health foods for the prevention and treatment of gastrointestinal discomfort. Based on frequency calculations, pharmacodynamic analysis, and recommended dosages, the combination of Dendrobium officinale compound extracts was determined. The raw materials for this food-medicine homology composition consist of Dioscorea opposita, Dendrobium officinale, Poria cocos, and Dolichos lablab. Subsequently, based on sensory evaluation and condition of the prepared herbal beverage, as well as cost accounting for various formulations, the proportions of the four food-medicine homology herbal formulas were optimized and determined. The gastric mucosal injury referred to in this invention specifically includes mucosal injury in the antrum of the stomach. Treatment or improvement of gastric mucosal injury includes improving gastric mucosal bleeding, reducing the damaged area, alleviating gastric tissue glandular structure disorder and inflammatory cell infiltration, increasing superoxide dismutase activity in the gastric mucosa, and reducing malondialdehyde content.

[0149] The embodiments described above are merely preferred embodiments of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications, improvements, and substitutions without departing from the concept of the present invention, and these all fall within the scope of protection of the present invention. Therefore, the scope of protection of this patent should be determined by the appended claims.

Claims

1. A compound extract of Dendrobium that regulates gastrointestinal function, characterized in that: It is made from the following medicinal and edible plant materials in the following weight ratios: 5-15 parts of Dendrobium officinale, 5-20 parts of Dioscorea opposita, 2-10 parts of Poria cocos, and 2-10 parts of Dolichos lablab; the extract is an aqueous extract; the extract is used to improve alcoholic or acetic acid-induced gastric mucosal damage.

2. The Dendrobium compound extract for regulating gastrointestinal function according to claim 1, characterized in that: It is made from the following medicinal and edible plant materials in the following weight ratio: 5 parts Dendrobium officinale, 7 parts Dioscorea opposita, 4 parts Poria cocos, and 4 parts Dolichos lablab.

3. A method for preparing a compound extract of Dendrobium that regulates gastrointestinal function, characterized in that: Includes the following steps: S1. Take dried Dendrobium officinale, Dioscorea opposita, Poria cocos and white hyacinth bean, grind them into powder, pass through a 40-80 mesh sieve, and calculate by weight parts: Dendrobium officinale 5-15 parts, Dioscorea opposita 5-20 parts, Poria cocos 2-10 parts, white hyacinth bean 2-10 parts; S2. Under water bath conditions of 70~100 ℃, add 5~15 times the volume of water and stir thoroughly for 3~6 h; S3. Filter the mixture through gauze to obtain the Dendrobium compound extract; The extract is used to improve alcoholic or acetic acid-induced gastric mucosal damage.

4. The method for preparing the Dendrobium compound extract with gastrointestinal function regulating effect according to claim 3, characterized in that: Includes the following steps: S1. Take dried Dendrobium officinale, Dioscorea opposita, Poria cocos, and white hyacinth bean, grind them into powder, pass them through a 60-mesh sieve, and calculate by weight: 5 parts Dendrobium officinale, 7 parts Dioscorea opposita, 4 parts Poria cocos, and 4 parts white hyacinth bean. S2. Under 85 ℃ water bath conditions, add 10 times the volume of water and stir thoroughly for 4 h; S3. The Dendrobium compound extract was obtained by filtration through 100-mesh gauze.

5. The application of a compound extract of Dendrobium that regulates gastrointestinal function, characterized in that: The Dendrobium compound extract according to any one of claims 1 or 2 is used to prepare health food products that improve alcoholic or acetic acid-induced gastric mucosal damage.

6. The application of the Dendrobium compound extract for regulating gastrointestinal function according to claim 5, characterized in that: The health food product can inhibit the decrease in superoxide dismutase content or the increase in malondialdehyde content caused by ethanol.

7. The application of the Dendrobium compound extract for regulating gastrointestinal function according to claim 5, characterized in that: The health food product can inhibit the decrease in motilin or gastrin levels caused by senna leaves.

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