A method for promoting rapid propagation of black currant seedlings

Abstract

The application belongs to the technical field of seedling breeding, and specifically discloses a method for promoting rapid breeding of Vaccinium uliginosum seedlings, and aims at solving the technical problems that the production cost of tissue culture technology is high, the efficiency is low, the popularization is difficult, and the domestic Vaccinium uliginosum seedling cultivation technology has not been reported. In the process of cultivating the Vaccinium uliginosum from seeds into seedlings, the seedlings are bred in a non-tissue culture manner, a semiconductor light-emitting diode plant growth lighting system is used as a light source, different light quality treatments are implemented according to different stages of the Vaccinium uliginosum seedling cultivation, the cultivation period of the Vaccinium uliginosum seedlings is significantly shortened, the cultivation from seeds to finished seedlings only needs 90 days, the side branch forming efficiency reaches 73.3%, the seedlings bred by the method have stable genetic traits and good quality, and the technology has important significance for promoting the breeding of the Vaccinium uliginosum seedlings.

Description

Technical Field

[0001] This invention relates to the field of seedling propagation technology, specifically to a method for promoting the rapid propagation of black blueberry seedlings. Background Technology

[0002] Black blueberry (Vaccinium myrtillus L.) is a wild shrub belonging to the genus Vaccinium in the family Ericaceae. Difficult seed germination and high cultivation requirements have become major obstacles to its successful propagation. It not only requires acidic soil environment and distribution under mixed larch-spruce or red pine coniferous forests, but also an altitude of 1800-2000 meters. Under natural conditions, the germination of black blueberry seeds is affected not only by abiotic factors such as temperature, humidity, and light, but also by biotic factors such as herbivores. Studies have shown that the number of naturally surviving seedlings in nature is extremely small. Their survival rate is closely related to the activities of herbivores such as grouse, sheep, and rodents. Seeds coated with fruit pulp have a seedling survival rate of up to 7%, and the time for seedling lateral branches to form is as long as 3 years or more, with an extremely slow growth rate. The existing cluster-growing black blueberries are propagated by rhizomes over many years. In China, black blueberries are only distributed in a few high-altitude areas in the Altay region, such as Habahe, Burqin, and Altay City, where there are less than 100 days without snow.

[0003] The germination and seedling development of black blueberry seeds are affected by factors such as humidity, temperature and light fluctuations. The germination process is long and the seedling growth rate is low. At present, foreign researchers use tissue culture technology for black blueberry seedling cultivation, while there are no reports on domestic black blueberry seedling cultivation technology. Therefore, developing low-cost, high-efficiency rapid propagation technology has become the best way to rapidly expand its population. Summary of the Invention

[0004] To address the technical problems of high production costs, low efficiency, and difficulty in promotion of tissue culture technology, and the lack of reported domestic cultivation techniques for black blueberry seedlings, this invention provides a method to promote the rapid propagation of black blueberry seedlings. By propagating black blueberries from seeds to seedlings using a non-tissue culture method, and employing a semiconductor light-emitting diode plant growth lighting system as the light source, different light quality treatments are implemented according to different stages of black blueberry seedling cultivation. This significantly shortens the cultivation cycle of black blueberry seedlings, requiring only 90 days from seed to finished seedling, with a lateral branching efficiency of 73.3%. Furthermore, the seedlings cultivated by this invention exhibit stable genetic traits and high quality.

[0005] To achieve the above technical effects, the present invention is implemented through the following technical solution.

[0006] This invention provides a method for promoting the rapid propagation of black blueberry seedlings, the method comprising:

[0007] Desuppress;

[0008] Disinfect the seeds of black blueberry;

[0009] sowing;

[0010] Interactive processing of light quality and occlusion;

[0011] The photosynthetic effect was measured on seedlings after light quality treatment.

[0012] The method for removing seed coat inhibition involves placing black blueberry seeds in three different pH solutions, N1, N2, and N3, with a pH range of 4–9, for 8–24 hours. The acid solution is adjusted with HCl, and the alkaline solution is adjusted with NaOH, wherein the pH of N1 is 4–5, the pH of N2 is 6–7, and the pH of N3 is 8–9.

[0013] The disinfection conditions are as follows: first treat with 70% ethanol for 1 min, then treat with 10% NaClO for 3-5 min, rinse with sterile water 3-5 times, then add 0.5 mg / mL GA3 (filtered and sterilized), and place at 4°C for 48 h.

[0014] The sowing conditions are as follows: Black blueberry seeds that have undergone seed coat removal and disinfection are evenly placed in sterilized peat moss for sowing.

[0015] The light quality conditions are as follows: the light intensity is 0–60 μmol / m². 2 ·s -1 Light intensity 0-4000 lx, illumination time 14 hours / day.

[0016] Furthermore, the light intensity of the light quality ranges from 5 to 40 μmol / m² during the seed germination and sprouting stages. 2 ·s -1 During the seedling growth stage, the concentration is 40–60 μmol / m 2 ·s -1 .

[0017] Furthermore, the light intensity is 300-3000 lx during the seed germination and sprouting period, and 2500-4000 lx during the seedling growth stage.

[0018] The shading treatment is set with three different shading levels: D1 0-30%, D2 30-60%, and D3 60-90%.

[0019] The photosynthetic effect of seedlings under light quality treatment was measured using chlorophyll fluorescence imaging.

[0020] The culture was terminated when the radicle reached 0.2 cm in length as the germination standard, and the number of seeds that germinated and sprouted was determined by the unfolding of the two cotyledons for each treatment. The culture was terminated when the cotyledons stopped unfolding for three consecutive days.

[0021] Through the above technical solutions, the present invention achieves the following technical effects:

[0022] The present invention provides a method for promoting the rapid propagation of black blueberry seedlings. By using a method of alternating treatment with different light qualities and shading conditions, the cultivation cycle of black blueberry seedlings is significantly shortened. It only takes 90 days from seed to finished seedling, and the lateral branch formation efficiency reaches 73.3%. The seedlings produced have the characteristics of stable genetic conversion and good quality. This technology is of great significance for promoting the propagation of black blueberry seedlings. Attached Figure Description

[0023] Figure 1 The graph shows the correlation analysis of various indicators of germination in black blueberry under different light quality and shading interaction conditions.

[0024] Figure 2 The graph shows the effect of different light qualities and shading interactions on the germination of black blueberry seeds.

[0025] Figure 3 The image shows the growth of black blueberries under different light qualities.

[0026] Figure 4 The graph shows the ETR-PAR photosynthetic response fitting curves of black blueberry seedlings under different light qualities. Detailed Implementation

[0027] The present invention will now be illustrated with examples; however, the present invention is not limited to the examples described below. All raw and auxiliary materials used in the present invention, as well as the selected microbial culture methods, are well known in the art. All percentages mentioned in the present invention are volume percentages unless otherwise specified.

[0028] The mature berries of the black blueberry used in this invention were collected on September 7th under pine needle forests at an altitude of 2000 meters in Habahe County. The berries were gently crushed to release the seeds, and then washed with sand for 16-24 hours. After washing with clean water 3-5 times, the seeds were dried in the shade at room temperature for 5-6 days and then stored at room temperature for later use.

[0029] This invention uses Microsoft Excel and SPSS for data processing and analysis, and Origin 2021, Sigmaplot 14, and Photoshop 2017 for correlation analysis, seed germination data, and image processing.

[0030] Example 1: Method for removing seed coat inhibition and disinfecting black blueberry seeds

[0031] Black blueberry seeds were placed in three different pH solutions (N1, N2, and N3) ranging from 4 to 9, with N1 at pH 4-5, N2 at pH 6-7, and N3 at pH 8-9. Each solution was treated for 8-24 hours to remove the seed coat inhibition. Afterward, the seeds were sterilized with 70% ethanol for 30 seconds, followed by 10% NaClO for 3-5 minutes. They were then rinsed 3-5 times with sterile deionized water, and finally, filtered sterilized 0.5 mg / mL GA3 was added. The mixture was then placed at 4°C for 48 hours for further sterilization. The treated black blueberry seeds were then evenly distributed in sterilized peat moss for sowing. The soil was kept moist by watering every other day.

[0032] Example 2: Methods for promoting rapid propagation of black blueberry seedlings

[0033] Six different light qualities (L1, L2, L3, L4, L5, L6) were set up, with the illumination quantum intensity of the light quality ranging from 0 to 60 μmol / m. 2 ·s -1 Three different shading efficiencies (D1, D2, D3) were used, with shading efficiencies ranging from 0% to 90%. The light intensity during seed germination and germination was 300–3000 lx, and the light intensity during seedling growth was 2500–3800 lx. The light duration was 14 h / day. Each treatment contained 90 seeds, and the results were replicated three times. Seed germination and sprouting were observed and recorded daily after 9 days of germination. The radicle length of 0.2 cm was used as the germination standard, and the unfolding of two cotyledons was used as the sprouting standard for each treatment. The study was terminated when the cotyledons stopped unfolding for three consecutive days. The germination and sprouting data were then statistically analyzed.

[0034] Example 3: Treatment for rapid germination of black blueberry seeds

[0035] The light quantum intensity of light quality (L1) is 21–26 μmol / m. 2 ·s -1 Under the condition of shading efficiency of (D1) 0-30% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0036] Example 4: Treatment for rapid germination of black blueberry seeds

[0037] The light quantum intensity of light quality (L1) is 21–26 μmol / m. 2 ·s -1Under the conditions of shading efficiency of (D2) 30-60% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0038] Example 5: Treatment for rapid germination of black blueberry seeds

[0039] The light quantum intensity of light quality (L1) is 21–26 μmol / m. 2 ·s -1 Under the conditions of shading efficiency (D3) of 60-90% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0040] Example 6: Treatment for rapid germination of black blueberry seeds

[0041] The light quantum intensity of light quality (L2) is 15–20 μmol / m 2 ·s -1 Under the condition of shading efficiency of (D1) 0-30% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0042] Example 7: Treatment for rapid germination of black blueberry seeds

[0043] The light quantum intensity of light quality (L2) is 15–20 μmol / m 2 ·s -1 Under the conditions of shading efficiency of (D2) 30-60% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0044] Example 8: Treatment for rapid germination of black blueberry seeds

[0045] The light quantum intensity of light quality (L2) is 15–20 μmol / m 2 ·s -1 Under the conditions of shading efficiency (D3) of 60-90% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0046] Example 9: Treatment for rapid germination of black blueberry seeds

[0047] The light quantum intensity of light quality (L3) is 40–45 μmol / m 2 ·s -1 Under the condition of shading efficiency of (D1) 0-30% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0048] Example 10: Treatment for rapid germination of black blueberry seeds

[0049] The light quantum intensity of light quality (L3) is 40–45 μmol / m 2 ·s -1 Under the conditions of shading efficiency of (D2) 30-60% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0050] Example 11: Treatment for rapid germination of black blueberry seeds

[0051] The light quantum intensity of light quality (L3) is 40–45 μmol / m 2 ·s -1 Under the conditions of shading efficiency (D3) of 60-90% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0052] Example 12: Treatment for rapid germination of black blueberry seeds

[0053] The light quantum intensity of light quality (L4) is 25–30 μmol / m. 2 ·s -1 Under the condition of shading efficiency of (D1) 0-30% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0054] Example 13: Treatment for rapid germination of black blueberry seeds

[0055] The light quantum intensity of light quality (L4) is 25–30 μmol / m. 2 ·s -1Under the conditions of shading efficiency of (D2) 30-60% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0056] Example 14: Treatment for rapid germination of black blueberry seeds

[0057] The light quantum intensity of light quality (L4) is 25–30 μmol / m. 2 ·s -1 Under the conditions of shading efficiency (D3) of 60-90% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0058] Example 15: Treatment for rapid germination of black blueberry seeds

[0059] The light quantum intensity of light quality (L5) is 45–50 μmol / m 2 ·s -1 Under the condition of shading efficiency of (D1) 0-30% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0060] Example 16: Treatment for rapid germination of black blueberry seeds

[0061] The light quantum intensity of light quality (L5) is 45–50 μmol / m 2 ·s -1 Under the conditions of shading efficiency of (D2) 30-60% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0062] Example 17: Treatment for rapid germination of black blueberry seeds

[0063] The light quantum intensity of light quality (L5) is 45–50 μmol / m 2 ·s -1 Under the conditions of shading efficiency (D3) of 60-90% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0064] Example 18: Treatment for rapid germination of black blueberry seeds

[0065] The light quantum intensity of light quality (L6) is 35–40 μmol / m. 2 ·s -1 Under the condition of shading efficiency of (D1) 0-30% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0066] Example 19: Treatment for rapid germination of black blueberry seeds

[0067] The light quantum intensity of light quality (L6) is 35–40 μmol / m. 2 ·s -1 Under the conditions of shading efficiency of (D2) 30-60% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0068] Example 20: Treatment for rapid germination of black blueberry seeds

[0069] The light quantum intensity of light quality (L6) is 35–40 μmol / m. 2 ·s -1 Under the conditions of shading efficiency (D3) of 60-90% and light duration of 14h / day, the rapid propagation of black blueberry from seed to bud stage was studied. Based on correlation analysis, 10 physiological indicators generated during the germination process of black blueberry seeds were analyzed.

[0070] Example 21: Calculation of each indicator

[0071] All time calculations are based on the time elapsed since the seeds were sown in the soil.

[0072] Start Germination Time (SGT): The time when the first seed germinates;

[0073] Germination Rate (GR)% = (Total number of germinated seeds / Number of tested seeds) * 100;

[0074] Germination potential (GE) = (total number of seeds germinating within a specific time period / number of seeds tested) * 100;

[0075] Mean Germination Time (MGT) = ∑(Gi Ti) / ∑G;

[0076] Germination Index (GI) = ∑G / Ti;

[0077] Germinated Seed Rate (GSR) % = Number of germinated seeds / Number of germinated seeds in the test;

[0078] Germinated Seed Energy (GSE)% = (Total number of seeds germinating within a specific time period / Number of germinated seeds among the tested seeds) × 100;

[0079] Start Sprout Time (SST): The time when the first seed germinates;

[0080] Germination rate (SproutRate, SR)% = (Total number of germinated seeds / Number of seeds tested) × 100;

[0081] Germination potential (SE) = (total number of seeds germinated within a specific time period / number of seeds tested) × 100;

[0082] Mean Sprout Time (MST) = ∑(Si Ti) / ∑S;

[0083] Sprout Index (SI) = ∑S / Ti;

[0084] In the above formula, Gi and Si refer to the number of newly germinated seeds at time Ti, where Ti is the corresponding observation time, and i = 10, 11, 12, ..., 35d.

[0085] Example 22: Analysis of various physiological indicators of rapid germination in black blueberries

[0086] Based on the different light quality and shading conditions used in Examples 3-20, the rapid propagation of black blueberry from seed to bud stage was studied. Correlation analysis was conducted, and 10 physiological indicators generated during black blueberry seed germination were analyzed. The results are as follows: Figure 1As shown, the test criterion for each indicator is p<0.01, and the darker the color and the larger the circle area, the stronger the correlation. The results show that most indicators reached a highly significant positive and negative correlation level. Among them, the decisive physiological indicators for rapid seed germination, the start time of germination (SST) and the mean time of germination (MST), showed a highly significant positive correlation with the start time of germination (SGT) and the mean time of germination (MGT), with correlation coefficients all above 0.80, and the correlation coefficient between SST and MST was as high as 0.93. SST and MST also showed significant negative correlations with germination index (GI), germination potential (SE), germination rate (SR), and germination index (SI). The correlation coefficients between SST and GI, SE, SR, and SI were 0.89, 0.92, 0.86, and 0.84, respectively; the correlation coefficients between MST and GI, SE, SR, and SI were 0.85, 0.87, 0.76, and 0.71, respectively, while the correlations between SST and MST and germination rate were low, only 0.65 and 0.5, respectively. This indicates that GI, SI, SR, SE, SST, and MST can be used as indicators to comprehensively evaluate the rapid germination process of black blueberry seeds.

[0087] Example 23: Effects of light quality and shading rate treatments on physiological parameters of black blueberry seed germination

[0088] The two-way ANOVA in Table 1 shows that, according to Examples 3-20, different light qualities, shading treatments, and their interaction significantly affected the physiological indicators of black blueberry seeds, including germination index, germination potential, and initial germination time. Furthermore, the interaction between these two treatments had a significant impact on each physiological indicator. See Appendix for the results. Figure 2 As shown, among all light quality treatments, shading had a significant effect on various physiological indicators, with the highest effect observed under treatment D3, significantly higher than D1 and D2. This indicates that 60-90% shading is optimal for the germination of black blueberry seeds, and excessively low shading rates significantly inhibit seed germination, with the efficiency of shading in the order of D3 > D1 > D2. Under the same shading rate, the effects of different light quality treatments on various physiological indicators differed significantly. In the D3 shading treatment, germination index and germination rate, as well as excessively low or high light quality variations, all inhibited the germination rate of black blueberry seeds. Physiological indicators such as the initial germination time were best under the L5 light quality condition. Therefore, the optimal shading conditions are found in treatments with 60-90% shading under D3 and light intensity of 45-50 μmol / m² under L5. 2 ·s -1 Under interactive conditions, it can promote the germination efficiency of black blueberry seeds and accelerate the germination rate.

[0089] Table 1. Bivariate analysis of variance (F) for various indicators of black blueberries.

[0090] Germination Index <![CDATA[6.96 * ]]> 80.91* 12.29* Germination start time <![CDATA[15.94 * ]]> 156.74* 11.07* Average germination time <![CDATA[7.94 * ]]> 227.37* 7.88* Germination potential <![CDATA[18.22 * ]]> 65.37* 6.71* Germination start time <![CDATA[51.37 * ]]> 211.34* 43.23* Average germination time <![CDATA[92.18 * ]]> 266.55* 85.26*

[0091] *p<0.0

[0092] Example 24: The effect of light quality on the rapid growth of black blueberry seedlings

[0093] Six different light qualities were set up, with the following: the light quantum intensity of L1 being 21–26 μmol / m². 2 ·s -1 The light quantum intensity of L2 is 15–20 μmol / m 2 ·s -1 The light quantum intensity of L3 is 40–45 μmol / m 2 ·s -1 The light quantum intensity of L4 is 25–30 μmol / m. 2 ·s -1 The light quantum intensity of L5 is 45–50 μmol / m 2 ·s -1 The light quantum intensity of L6 is 35–40 μmol / m 2 ·s -1 Two seedlings with fully expanded cotyledons were placed under each light quality. After 60 days, the number of leaves, plant height, internode distance, stem diameter, and number of lateral branches were counted. The photosynthetic effect of the seedlings under each light quality treatment was measured using chlorophyll fluorescence imaging.

[0094] Table 2 shows that light quality L5 is far superior to other light quality light qualities in terms of physiological indicators such as leaf number, plant height, internode distance, and stem diameter, with a lateral branch formation rate as high as 73.33%. The photosynthetic response fitting curve of PAR-ETR was obtained using chlorophyll fluorescence imaging; the results are shown in the appendix. Figure 4 This indicates that its photosynthetic transport efficiency is high under L5 illumination with quantum intensities of 45–50 μmol / m². 2 ·s -1 The following has the highest efficiency; see the appendix for results. Figure 3 This further verifies that light quality L5 is a light quality with a significant rapid propagation effect.

[0095] Table 2 Biomass of black blueberries under different light quality conditions

[0096]

[0097] This invention promotes the propagation of black blueberry seedlings from seeds using a non-tissue culture method. It employs a semiconductor light-emitting diode (LED) plant growth lighting system as the light source and implements different light quality treatments according to different stages of seedling cultivation. Compared to existing tissue culture methods, which result in seedlings taking up to three years or more to form lateral branches and exhibiting extremely slow growth, this invention provides a method for rapidly propagating black blueberry seedlings. This significantly shortens the cultivation cycle, requiring only 90 days from seed to finished seedling, with a lateral branch formation efficiency of 73.3%. Furthermore, the seedlings cultivated using this method exhibit stable genetic traits and high quality. This technology is of great significance for promoting the propagation of black blueberry seedlings. Therefore, this application significantly shortens the seed germination and cultivation cycle of black blueberry while also significantly improving the efficiency of lateral branch formation.

[0098] The embodiments described above are only some, not all, of the embodiments of the present invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments. All other embodiments obtained by those skilled in the art through related deductions and substitutions based on the inventive concept, without inventive effort, are within the scope of protection of the present invention.

Claims

1. A method for promoting the rapid propagation of black blueberry seedlings, characterized in that, The method for rapid propagation of seedlings includes: removing inhibition from the seed coat of black blueberry; disinfection; sowing; alternating light quality and shading treatment; and measuring the photosynthetic effect of seedlings after light quality treatment. The method for removing seed coat inhibition involves placing black blueberry seeds in three different pH solutions, N1, N2, and N3, with a pH range of 4-9, for 8-24 hours. The acid solution is adjusted with HCl, and the alkaline solution is adjusted with NaOH, where the pH of N1 is 4-5, the pH of N2 is 6-7, and the pH of N3 is 8-9. The disinfection conditions are as follows: first treat with 70% ethanol for 1 min, then treat with 10% NaClO for 3-5 min, rinse with sterile water 3-5 times, then add 0.5 mg / mL GA3 (filtered and sterilized), and place at 4℃ for 48 h. The light quantum intensity of the light quality is 45-50 μmol / m²·s during the seed germination and sprouting period. -1 During the seedling growth stage, the concentration is 45-50 μmol / m 2 ·s -1 ; The light intensity is 300-3000 lx during the seed germination and germination period, and 2500-4000 lx during the seedling growth stage; The shading treatment level is 60-90%; The sowing conditions are as follows: Black blueberry seeds that have undergone seed coat removal and disinfection are evenly placed in sterilized peat moss for sowing. The photosynthetic effect of seedlings was measured using chlorophyll fluorescence imaging.

2. The method for promoting rapid propagation of black blueberry seedlings as described in claim 1, characterized in that, The cultivation ended when the radicle reached 0.2 cm in length as the germination standard, and the number of seeds that germinated and sprouted was determined by the unfolding of the two cotyledons. Cultivation was terminated when the cotyledons stopped unfolding for three consecutive days.

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