Tea-containing feed additive and use thereof
By preparing tea-containing feed additives and combining them with the fermentation treatment of summer and autumn tea and Bacillus subtilis, the problem of unstable egg production performance in laying hens in existing technologies has been solved, thereby improving the egg production rate and egg quality and enhancing the economic benefits of laying hen farming.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
- Filing Date
- 2024-07-17
- Publication Date
- 2026-08-04
AI Technical Summary
The existing technology of directly adding Bacillus subtilis and green tea to feed has no significant overall breeding effect, and its impact on the egg production performance of laying hens is unstable, making it difficult to comprehensively improve the economic benefits of laying hen farming.
A tea-containing feed additive is prepared by using fresh summer and autumn tea feed, fermented and sterilized summer and autumn tea feed, and Bacillus subtilis compound microbial powder, combined with an attractant. Through specific fermentation and sterilization treatment, a compound microbial powder is formed for the auxiliary feeding of laying hens.
It effectively promotes egg production rate, improves egg quality and gut health, and comprehensively enhances egg production rate and economic benefits.
Smart Images
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Abstract
Description
Technical Field
[0001] This invention relates to the field of laying hen feed formulation technology, specifically to a tea-containing feed additive and its application. Background Technology
[0002] With the improvement of living standards, egg products are becoming increasingly important in daily diets, and the scale of egg-laying hen farming is also growing larger. In order to improve the economic benefits of egg-laying hen farming, promote egg production, and improve the quality of egg production, this is an important research direction for large-scale egg-laying hen farming at present.
[0003] Green tea contains various bioactive substances such as tea polyphenols, catechins, and theanine, which help improve the health of humans and animals, enhance livestock and poultry production performance, meat and egg quality, and improve antioxidant and disease resistance. Bacillus subtilis is a widely used microecological preparation in the current livestock industry. It can produce spore structures, has strong resistance, and is easy to process, store, and transport. After entering the animal intestines, Bacillus subtilis can produce a large number of physiologically active substances to inhibit the growth of pathogens and promote the growth of beneficial bacteria. However, the overall breeding effect of feed directly adding microbial agents and green tea is not significant, and the impact on egg production performance in laying hens is also unstable. Therefore, based on this, research can be conducted on the effects of the combined addition of microbial agents and green tea, as well as the ratio of other components, on egg production in laying hens to comprehensively improve the overall economic benefits of laying hen farming. Summary of the Invention
[0004] To address the shortcomings of existing technologies, this invention provides a tea-containing feed additive and its application, which can effectively promote the egg production rate of laying hens and further improve the quality of eggs, thereby comprehensively enhancing the economic benefits of laying hen farming.
[0005] To achieve the above objectives, the technical solution of the present invention is implemented through the following technical solution:
[0006] A tea-containing feed additive, wherein the tea-containing feed additive is prepared by mixing the following substances in parts by weight: 300-400 parts of fresh summer and autumn tea leaves, 400-500 parts of fermented and sterilized summer and autumn tea leaves, 80-100 parts of Bacillus subtilis compound powder, and 15-20 parts of attractant; wherein the fermented and sterilized summer and autumn tea leaves are obtained by fermenting fresh summer and autumn tea leaves with yeast and bifidobacteria and then sterilizing them; the Bacillus subtilis compound powder is obtained by mixing Bacillus subtilis fermentation bacteria with soybean flour for secondary fermentation and then drying.
[0007] Preferably, the preparation method of the summer and autumn tea fermentation sterilization material includes the following steps:
[0008] S1-1. Wash the fresh tea leaves from summer and autumn, add water and grind them into a paste to obtain a prepared paste for later use.
[0009] S1-2. Inoculate the prepared slurry with yeast culture and ferment for 2-3 days to obtain pretreated fermented material for later use.
[0010] S1-3. Add Bifidobacterium bacterial solution to the pretreated fermentation material and continue fermentation for 2-3 days to obtain secondary fermentation material for later use.
[0011] S1-4. Sterilize the secondary fermentation material at 121℃ for 15-20 minutes, then remove and dry it to obtain the summer and autumn tea fermentation sterilized material.
[0012] Preferably, the bacterial count in the yeast culture during steps S1-2 is 10. 8 -10 9 The yeast culture concentration is 0.4%-0.6% of the total mass of fresh tea leaves.
[0013] Preferably, the bacterial count of the Bifidobacterium culture in steps S1-3 is 10. 6 -10 8 The concentration of Bifidobacterium was 100 cells / mL, and the amount of Bifidobacterium bacterial solution added was 0.3%-0.5% of the total mass of fresh tea leaves.
[0014] Preferably, the drying temperature in steps S1-4 is 50-60℃, and the drying is carried out until the moisture content is ≤10%.
[0015] Preferably, the preparation method of the Bacillus subtilis compound powder includes the following steps:
[0016] S2-1. Prepare Bacillus subtilis activation solution by adding water to Bacillus subtilis for later use.
[0017] S2-2. Grind soybeans with water into a paste, dry and pulverize it through a 60-mesh sieve to obtain soybean powder for later use.
[0018] S2-3. Add soybean flour to Bacillus subtilis activation solution, continue fermentation for 3-6 days, then dry to obtain Bacillus subtilis compound powder, and control the Bacillus subtilis content in the powder to 2×10⁻⁶. 9 -4×10 9 per g.
[0019] Preferably, the bacterial count in the Bacillus subtilis activation solution in step S2-1 is 10. 8 -10 9 per mL.
[0020] Preferably, the drying method in steps S2-3 is to dry at 70°C for 3-4 hours.
[0021] Preferably, the palatability enhancer is prepared by mixing cinnamon, pepper, fennel and licorice in a mass ratio of 40-50:28-32:18-22:4-6 and then pulverizing them.
[0022] Preferably, the tea-containing feed additive is used as an auxiliary feed for laying hens, and the specific application method is to add 3-8 kg of tea-containing feed additive per ton of laying hen daily feed.
[0023] This invention provides a tea-containing feed additive and its application, which has the following advantages compared with the prior art:
[0024] This invention uses fresh tea leaves, fermented tea leaves, and compound Bacillus subtilis as raw materials to prepare feed additives, which can effectively improve chicken egg production performance, regulate chicken lipid metabolism, improve egg quality and intestinal health, increase the overall egg production rate of laying hens, and further increase the diameter and weight of eggs laid by laying hens, thereby comprehensively improving the economic benefits of laying hen farming with little change in feed consumption. Detailed Implementation
[0025] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0026] The yeast strain described below is *Saccharomyces boulardii*, purchased from Xi'an Tianguangyuan Biotechnology Co., Ltd. The yeast culture was prepared by adding *Saccharomyces boulardii* to water and activating at room temperature until the bacterial count reached 10⁻⁶. 8 -10 9 The Bifidobacterium was Bacillus lactis JYBR-190, purchased from Shandong Dawei Pharmaceutical Co., Ltd., and the Bifidobacterium culture was prepared by activating the Bifidobacterium with water and controlling the bacterial count at 10. 6 -10 8 CFU / mL; Bacillus subtilis was Bacillus subtilis CH-001, preservation number CCTCC NO: M2011111, purchased from the Biological Laboratory of Hunan Agricultural University.
[0027] The following palatability enhancer is prepared by mixing cinnamon, pepper, fennel, and licorice in a mass ratio of 45:30:20:5 and then pulverizing them.
[0028] Example 1:
[0029] I. Preparation of processed summer and autumn tea leaves:
[0030] 1. Fermentation and sterilization material A:
[0031] (1) Wash the fresh tea leaves of summer and autumn tea, add twice the amount of water and grind them into a paste to obtain a prepared paste for later use;
[0032] (2) Inoculate the prepared slurry with yeast culture of 0.5% of the total mass of fresh tea leaves, and ferment at 40±2℃ for 3 days to obtain pretreated fermented material for later use.
[0033] (3) Add 0.4% of Bifidobacterium liquid of fresh tea leaves to the pretreated fermentation material and continue fermentation at 40±2℃ for 2 days to obtain secondary fermentation material for later use.
[0034] (4) The secondary fermentation material was sterilized at 121℃ for 18 minutes, and then dried at 55℃ until the moisture content was ≤8% to obtain the summer and autumn tea fermentation sterilization material A.
[0035] 2. Fermentation and sterilization material B:
[0036] (1) Wash the fresh tea leaves of summer and autumn tea, add twice the amount of water and grind them into a paste to obtain a prepared paste for later use;
[0037] (2) Inoculate the prepared slurry with yeast culture of 0.5% of the total mass of fresh tea leaves, and ferment at 40±2℃ for 5 days to obtain fermented material for later use.
[0038] (3) Sterilize the fermentation material at 121℃ for 18 minutes, then take it out and dry it at 55℃ until the moisture content is ≤8% to obtain the summer and autumn tea fermentation sterilization material B.
[0039] 3. Fermentation and sterilization material C:
[0040] (1) Wash the fresh tea leaves of summer and autumn tea, add twice the amount of water and grind them into a paste to obtain a prepared paste for later use;
[0041] (2) Inoculate the prepared slurry with 0.4% Bifidobacterium bacterial solution of fresh tea leaves and ferment it at 40±2℃ for 5 days to obtain fermented material for later use;
[0042] (4) The secondary fermentation material was sterilized at 121℃ for 18 minutes, and then dried at 55℃ until the moisture content was ≤8% to obtain the summer and autumn tea fermentation sterilization material C.
[0043] 4. Fermented tea leaves:
[0044] (1) Wash the fresh tea leaves of summer and autumn tea, add twice the amount of water and grind them into a paste to obtain a prepared paste for later use;
[0045] (2) Inoculate the prepared slurry with yeast culture of 0.5% of the total mass of fresh tea leaves, and ferment at 40±2℃ for 3 days to obtain pretreated fermented material for later use.
[0046] (3) Add 0.4% of the total mass of fresh tea leaves to the pre-treated fermented material and continue fermentation at 40±2℃ for 2 days to obtain secondary fermented material. Dry at 55℃ until the moisture content is ≤8% to obtain fermented tea.
[0047] II. Preparation of Bacillus subtilis powder:
[0048] 1. Preparation of bacterial powder A:
[0049] (1) Bacillus subtilis was activated with water to prepare Bacillus subtilis activation solution for later use;
[0050] (2) Grind soybeans with water into a paste, dry and pulverize it, and pass it through a 60-mesh sieve to obtain soybean powder for later use;
[0051] (3) Add soybean flour to Bacillus subtilis activation solution, ferment for 5 days, and then dry at 70℃ for 4 hours to obtain Bacillus subtilis compound powder, with the Bacillus subtilis content in the powder controlled at 2×10⁻⁶. 9 -4×10 9 per g.
[0052] 2. Preparation of bacterial powder B:
[0053] (1) Bacillus subtilis was activated with water to prepare Bacillus subtilis activation solution for later use;
[0054] (2) After continuous fermentation of the activation solution, centrifuge to obtain bacterial sludge for later use;
[0055] (3) Grind soybeans with water into a paste, dry and pulverize it, and pass it through a 60-mesh sieve to obtain soybean powder for later use;
[0056] (4) After mixing the bacterial mud with soybean flour and stirring evenly, dry it at 70℃ for 3 hours to obtain Bacillus subtilis compound bacterial powder, and control the content of Bacillus subtilis in the bacterial powder to be 2×10. 9 -4×10 9 per g.
[0057] Example 2:
[0058] Preparation of tea-containing feed additives:
[0059] (1) Prepare the ingredients according to the following weight proportions:
[0060] 350 portions of fresh summer and autumn tea leaves, 450 portions of processed summer and autumn tea materials, 90 portions of Bacillus subtilis powder, and 18 portions of attractant.
[0061] (2) After crushing the fresh summer and autumn tea leaves, mix them with the summer and autumn tea processing material, Bacillus subtilis powder, and attractant, and stir evenly to obtain a tea-containing feed additive.
[0062] Using the above preparation method, different summer and autumn tea treatment materials and Bacillus subtilis powder were selected according to Table 1 below to prepare tea-containing feed additives for different experimental groups:
[0063] Table 1
[0064] Experimental group 1 Fermentation and sterilization material A Bacterial powder A Experimental group 2 Fermentation and sterilization material B Bacterial powder A Experimental group 3 Fermentation sterilization material C Bacterial powder A Experimental group 4 Fermented tea Bacterial powder A Experimental group 5 Fermentation and sterilization material A Bacterial powder B
[0065] Comparative Example 1:
[0066] Preparation of tea-containing feed additives:
[0067] (1) Prepare the ingredients according to the following weight proportions: 800 parts of fresh summer and autumn tea leaves, 90 parts of Bacillus subtilis powder, and 18 parts of attractant;
[0068] (2) After crushing the fresh summer and autumn tea leaves, mix them with Bacillus subtilis powder and attractant, and stir evenly to obtain a tea-containing feed additive.
[0069] Detection:
[0070] Select the same batch of Hy-Line Brown laying hens and use the feed shown in Table 2 below as the basal diet:
[0071] Table 2
[0072] Metabolizable energy (ME, MJ / kg) 11.30 corn(%) 60.2 Crude protein (%) 15.0 Soybean meal (43%) 23.8 calcium(%) 3.90 Stone powder (%) 9.0 Available phosphorus (%) 0.35 Premix (%) 5.0 Lysine (%) 0.77 Soybean oil (%) 2.0 Methionine (%) 0.38 Threonine (%) 0.61 NaCl (%) 0.16
[0073] The laying hens were divided into 6 groups of 500 each, with free access to feed and water. The feed for each group was set as shown in Table 3 below:
[0074] Table 3
[0075] 1 995kg of ordinary daily ration + 5kg of experimental group 1 containing tea feed additive 2 995kg of ordinary daily ration + 5kg of experimental group 2 containing tea feed additive 3 995kg of ordinary daily ration + 5kg of experimental group 3 containing tea feed additive 4 995kg of ordinary daily ration + 5kg of experimental group 4 containing tea feed additive 5 995kg of ordinary daily ration + 5kg of experimental group 5 containing tea feed additive 6 1000kg ordinary daily ration
[0076] 1. After 500 days of rearing, the average egg production rate, average egg weight, and average daily feed consumption of each group of laying hens were recorded. The specific results are shown in Table 4 below:
[0077] Table 4
[0078] 1 84.8±3.6 61.8±0.5 123.5±6.2 2 83.5±5.0 61.0±0.4 125.2±4.1 3 82.7±6.2 61.2±0.5 122.7±6.0 4 82.4±4.6 60.8±0.3 124.5±3.6 5 83.9±5.7 61.3±0.4 123.4±4.8 6 81.3±6.8 60.3±0.7 120.7±4.2
[0079] 2. Five hens with similar growth conditions were randomly selected from each group of laying hens raised for 500 days for blood biochemical analysis. The specific results are shown in Table 5 below:
[0080] Table 5
[0081]
[0082]
[0083] 3. Randomly select 200 eggs from each group of laying hens for quality testing. The specific results are shown in Table 6 below:
[0084] Table 6
[0085]
[0086] The above tests show that feeding the laying hens with the additives from Experimental Group 1 can effectively improve their egg production performance and also play a certain role in regulating chicken lipid metabolism.
[0087] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims
1. A tea-containing feed additive, characterized in that, The tea-containing feed additive is prepared by mixing the following substances in parts by weight: 300-400 parts of fresh summer and autumn tea leaves, 400-500 parts of fermented and sterilized summer and autumn tea leaves, 80-100 parts of Bacillus subtilis compound powder, and 15-20 parts of attractant; The preparation method of the summer and autumn tea fermentation sterilization material includes the following steps: S1-1. Wash the fresh tea leaves from summer and autumn, add water and grind them into a paste to obtain a prepared paste for later use. S1-2. Inoculate the prepared slurry with yeast culture and ferment for 2-3 days to obtain pretreated fermented material for later use. S1-3. Add Bifidobacterium bacterial solution to the pretreated fermentation material and continue fermentation for 2-3 days to obtain secondary fermentation material for later use. S1-4. Sterilize the secondary fermentation material at 121℃ for 15-20 minutes, then take it out and dry it to obtain the summer and autumn tea fermentation sterilization material. The preparation method of the Bacillus subtilis compound powder includes the following steps: S2-1. Prepare Bacillus subtilis activation solution by adding water to Bacillus subtilis for later use. S2-2. Grind soybeans with water into a paste, dry and pulverize it through a 60-mesh sieve to obtain soybean powder for later use. S2-3. Add soybean flour to Bacillus subtilis activation solution, continue fermentation for 3-6 days, then dry to obtain Bacillus subtilis compound powder, and control the Bacillus subtilis content in the powder to 2×10⁻⁶. 9 -4×10 9 per g.
2. The tea-containing feed additive according to claim 1, characterized in that: The bacterial count in the yeast culture during steps S1-2 is 10. 8 -10 9 The yeast culture volume is 0.4%-0.6% of the total mass of fresh tea leaves.
3. The tea-containing feed additive according to claim 1, characterized in that: The bacterial count of the Bifidobacterium culture in steps S1-3 is 10. 6 -10 8 The concentration of Bifidobacterium was 0.3%-0.5% of the total mass of fresh tea leaves.
4. The tea-containing feed additive according to claim 1, characterized in that: The drying temperature in steps S1-4 is 50-60℃, and the drying is carried out until the moisture content is ≤10%.
5. The tea-containing feed additive according to claim 1, characterized in that: The bacterial count in the Bacillus subtilis activation solution in step S2-1 is 10. 8 -10 9 per mL.
6. The tea-containing feed additive according to claim 1, characterized in that: The drying method in step S2-3 is to dry at 70°C for 3-4 hours.
7. The tea-containing feed additive according to claim 1, characterized in that: The palatability enhancer is prepared by mixing cinnamon, pepper, fennel, and licorice in a mass ratio of 40-50:28-32:18-22:4-6 and then pulverizing the mixture.
8. An application of the tea-containing feed additive as described in any one of claims 1-7, characterized in that: The tea-containing feed additive is used as an auxiliary feed for laying hens, and the specific application method is to add 3-8 kg of tea-containing feed additive per ton of laying hen daily feed.