A traditional Chinese medicine composition for treating gout and application thereof

Abstract

The present application belongs to the technical field of veterinary medicine, and particularly relates to a traditional Chinese medicine composition for treating gout in geese and application thereof in preparation of a medicine or a pharmaceutical preparation for treating gout in geese. The traditional Chinese medicine composition comprises the following components in parts by weight: cassia twig 30-60 parts, sulfur 15-30 parts, cyperus 10-20 parts, white peony root 10-20 parts, and croton 5-10 parts. The preparation method comprises the following steps: (1) tearing and crushing cassia twig, cyperus, white peony root and croton respectively to obtain coarse tablets of each raw material; and (2) mixing the coarse tablets of each raw material with sulfur, crushing and sieving the mixture to obtain the traditional Chinese medicine composition for treating gout in geese. The traditional Chinese medicine composition prepared by the present application is inexpensive and effective, green and pollution-free, and is a practical Chinese medicine prescription that can be actually afforded in the breeding process, with a cost of less than 60 yuan per 1 kg, and has good market application and promotion value.

Description

Technical Field

[0001] This invention belongs to the field of veterinary drug technology, specifically relating to a traditional Chinese medicine composition for treating gout in geese, and its application in the preparation of drugs or pharmaceutical preparations for treating gout in geese. Background Technology

[0002] Gout in geese is a contagious disease that commonly affects goslings under three weeks old. Its main necropsy finding is severe urate deposition in multiple organs and tissues, with a mortality rate as high as 30%–80%. Current research on the etiology of goose goose goose disease generally falls into three categories: One view holds that astrovirus (AstV), a single-stranded, positive-sense, non-enveloped RNA virus, is the primary cause of the high incidence of goose goose goose disease. Many studies have demonstrated through virus isolation and regression experiments that this virus can indeed replicate the pathological model of goose goose goose disease. Furthermore, it is claimed that vaccines made from this virus, and egg yolk antibodies prepared from the same vaccine, offer protection against goose goose goose disease with a protection rate exceeding 95%. However, clinical applications have shown that the protection rate of this vaccine is significantly reduced, falling below 60%. Therefore, to date, no officially approved goose astrovirus vaccines or antibodies have been released. Another view argues that goose goose goose disease is a syndrome caused by multiple factors, as these factors can, to some extent, mimic the pathological model of goose goose goose disease. For example, nutritional imbalances such as excessive protein content and vitamin deficiency in feed can lead to gout in geese; mycotoxin poisoning and improper medication use can also cause gout; avian influenza, hepatitis viruses, and gosling plague can also cause liver and kidney damage, leading to urate metabolism disorders and inducing gout. While the above analyses of causes have some merit, implementing corresponding measures based solely on these causes for the prevention and control of gout in geese has not achieved ideal results. To date, there is still a lack of effective measures on the market to prevent and control gout in geese.

[0003] Analyzing this disease from the dialectical perspective of Traditional Chinese Medicine (TCM) theory can greatly simplify the etiology of gout, a multi-causative disease in geese. This allows for a simpler approach, focusing on the animal's own perspective rather than the virus or fungus, to develop effective prevention and control measures. Some TCM veterinary researchers believe that gout in geese belongs to the category of damp-heat syndrome and recommend the use of cooling herbs such as Coptis chinensis, Phellodendron chinense, Smilax glabra, Dioscorea hypoglauca, and Plantago asiatica, or diuretics. Some have even attempted treatments combining these with Western diuretics or anti-inflammatory drugs such as furosemide and dexamethasone, or using transfer factor and interferon. However, clinical observations have shown no ideal results. Currently, research is underway on antibodies against goose astrovirus, which have proven effective, but require injection, making clinical use inconvenient. Therefore, the research of a safe, effective, convenient, and economical drug for the prevention and treatment of gout in geese is urgently needed. Summary of the Invention

[0004] In view of the problems and deficiencies in the existing technology, the present invention aims to provide a traditional Chinese medicine composition for treating gout and its application.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] In a first aspect, the present invention provides a traditional Chinese medicine composition for treating gout, the traditional Chinese medicine composition comprising, by weight, the following components: 30-60 parts of cinnamon twig, 15-30 parts of sulfur, 10-20 parts of cyperus rhizome, 10-20 parts of white peony root, and 5-10 parts of croton seed.

[0007] Preferably, the traditional Chinese medicine composition comprises the following components by weight: 60 parts cinnamon twig, 30 parts sulfur, 20 parts cyperus rhizome, 20 parts white peony root, and 10 parts croton seed.

[0008] Secondly, the present invention provides a method for preparing a traditional Chinese medicine composition for treating gout, comprising the following steps:

[0009] (1) Cinnamon twig, Cyperus rhizome, white peony root, and croton seed are shredded separately to obtain coarse slices of each raw material;

[0010] (2) After mixing the raw materials with sulfur, crush and sieve the mixture to obtain the Chinese medicine composition for treating gout.

[0011] Preferably, the longest size of the raw material tablets in step (1) does not exceed 3 cm.

[0012] Preferably, the sieving in step (2) uses an 80-mesh sieve.

[0013] Thirdly, the present invention provides a medicament for treating gout in geese, comprising the traditional Chinese medicine composition for treating gout in the first aspect and pharmaceutically acceptable excipients.

[0014] Preferably, the drug is an oral dosage form; more preferably, the oral dosage form is a powder.

[0015] Preferably, the method of using the drug is as follows: the powder is added to the feed, and the dosage per gosling is 1.0 to 2.0g per day, based on the feed intake of the goslings.

[0016] Fourthly, the present invention provides the use of the traditional Chinese medicine composition described in the first aspect in the preparation of a medicament or pharmaceutical preparation for treating gout.

[0017] Fifthly, the present invention provides the use of the drug described in the third aspect in the preparation of a pharmaceutical formulation for treating gout.

[0018] The components of the traditional Chinese medicine composition of this invention can be purchased from commercially available Chinese medicinal materials.

[0019] Gout in geese primarily affects goslings. Currently, goose farming in China is intensive, with goslings often kept indoors from 1 to 10 days old to accelerate growth and survival rates. In cold weather, this can extend to over 15 days. During this period, they are provided with water via waterers and fed complete feed for broiler ducks or chickens (with crude protein content exceeding 24%), with virtually no green fodder. Those with the means may release them outdoors when temperatures are slightly higher (around 10°C), and some may have ponds for activity. This farming practice results in limited activity for goslings aged 5 to 20 days, coupled with a diet high in protein and energy. The disease is most prevalent from October to April of the following year, with milder symptoms or no symptoms during warmer seasons. Affected geese exhibit lethargy, excrete white, lime-like feces, lose appetite, and become listless. Autopsy revealed large amounts of white urate crystals adhering to the subcutaneous tissue, joints, and serosal surfaces of internal organs, most commonly found in the liver, kidneys, heart, and joints. In severe cases, urate crystal deposits were also observed in the gallbladder, mesentery, and outer wall of the intestines. This indicates the disease is due to cold-dampness stagnation and phlegm obstruction. Treatment focuses on warming yang, resolving dampness, regulating qi, and nourishing the liver, supplemented with croton seed to dispel cold accumulation and resolve stagnation, clearing the pathways for the body to eliminate cold-dampness stagnation and unblock phlegm obstruction.

[0020] In the formula,

[0021] Cinnamon twig: The tender twig of the cinnamon tree (Cinnamomum cassia Presl), a plant in the Lauraceae family. It tastes bitter and sweet, and is warm in nature. It enters the bladder, heart, and lung meridians. Functions: Induces sweating and relieves muscle tension, warms the meridians and unblocks the channels, and assists yang qi. In this formula, it is used to allow yang qi to circulate throughout the body and resolve dampness and turbidity.

[0022] Sulfur: "Sulfur is the essence of fire," and is one of the most potent kidney-warming drugs, and it is safe to take. However, attention should be paid to the grade of the raw material, using sublimated sulfur, strictly controlling the heavy metal content, and choosing flake form for easy processing and storage. In this formula, it warms the kidney yang, enhances the source of yang energy in the body, and allows the yang heat to rise continuously. With the assistance of cinnamon twig and cyperus rhizome, it dissolves and disperses phlegm and dampness stagnation in the body.

[0023] Cyperus rotundus L., a plant in the Cyperaceae family, is the rhizome of this plant. It is also known as Xiangfuzi (from *Tang Materia Medica*) and Shacao Gen (from *Mingyi Bielu*). It has a pungent, slightly bitter, and sweet taste, and is neutral in nature. It enters the liver and triple burner meridians. Its functions include regulating qi and relieving depression, alleviating pain, and regulating menstruation. It is primarily used to treat liver and stomach disharmony, qi stagnation, and phlegm accumulation in the abdominal cavity. In this formula, it is used to unblock the meridians obstructed by phlegm accumulation, allowing the warming qi of sulfur and cinnamon twigs to circulate throughout the body.

[0024] White peony root: The dried root of Paeonia lactiflora Pall., a plant in the Ranunculaceae family. Also known as white peony root (according to *Pharmacopoeia of Chinese Herbal Medicines*). It tastes bitter and sour, and is slightly cold in nature. It enters the liver and spleen meridians. It nourishes blood and softens the liver, relieves pain, and astringes yin to stop sweating. Main indications: pain in the chest, abdomen, and hypochondrium; spasms and pain in the limbs; diarrhea and abdominal pain; headache; dizziness; spontaneous sweating and night sweats. In this formula, when used together with Cyperus rotundus, it not only soothes the liver and regulates qi, but also softens the liver, nourishes blood, and relieves pain. It can greatly alleviate the symptoms of liver blood stasis and deficiency of body fluids and blood.

[0025] Croton seed: The mature seed of the croton tree (Croton tiglium L.), a member of the Crotonaceae family. It is pungent, hot in nature, and highly toxic. It enters the stomach, large intestine, and lung meridians. Functions: Clears cold accumulation, opens the orifices, eliminates phlegm, and promotes urination. It is used to treat cold accumulation and stagnation, abdominal distension and pain, blood stasis, phlegm accumulation, and diarrhea. In this formula, it accelerates the elimination of accumulated cold phlegm, clearing the pathway for the overall elimination of cold and dampness. Unlike Poria, Alisma, and Akebia, which, although having diuretic and dampness-eliminating effects, would worsen the already depleted body fluids due to stagnation in patients with severe cold and dampness accumulation, the warming nature of croton seed accelerates the elimination of cold accumulation.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0027] 1. This invention, guided by the principles of traditional Chinese medicine (TCM) syndrome differentiation, and combined with the common living habits of goslings under intensive farming conditions and the characteristics of goose gout, innovatively differentiates gout in goslings. Based on the results of this differentiation, a preventative and therapeutic TCM formula is developed, resulting in an effective TCM composition for the prevention and treatment of this disease: Sulfur is the principal ingredient, warming the kidneys and transforming yang, providing a heat source for dispelling cold and dampness; Cinnamon twig is the assistant ingredient, supplementing and clearing the yang qi in the meridians, and combined with the heat of sulfur, warming the whole body; Cyperus rotundus and Croton tiglium are the guiding ingredients, directing the medicinal power to the site of cold phlegm and blood stasis, clearing the pathways for the expulsion of cold phlegm and dampness; Peony root is added to nourish the blood and soften the liver, ensuring that the warming herbs in the formula are not too drying and could damage the internal organs or deplete body fluids. Using this entire formula together allows the yang qi in the body to flow freely, eliminating cold, dampness, and phlegm obstruction, naturally restoring health.

[0028] 2. The traditional Chinese medicine composition prepared in this invention showed significant intervention effects on various related indicators in the goose gout model experiment of the examples, reducing morbidity and mortality. In actual clinical application experiments, it showed superiority over the method of treating goose gout with sodium bicarbonate + transfer factor, and could also improve the weight gain rate of goslings to a certain extent.

[0029] 3. The traditional Chinese medicine composition prepared by this invention is inexpensive, effective, green and pollution-free. It is a practical and affordable traditional Chinese medicine formula for breeding. The cost per kg is less than 60 yuan, and it has good market application and promotion value. Attached Figure Description

[0030] Figure 1 The graph shows the survival rate of each group of experimental geese after viral challenge in Experiment Example 1.

[0031] Figure 2 The graph shows the serum uric acid results of each group of experimental geese after viral challenge in Experiment Example 1.

[0032] Figure 3 The image shows the serum creatinine results of each group of experimental geese after viral challenge in Experiment Example 1. Detailed Implementation

[0033] The following embodiments are only for further elaboration of the present invention. It should be noted that all techniques and scientific terms used in this invention, unless otherwise stated, have the same meaning as those in the technical field to which this invention pertains. Experimental methods in the following embodiments that do not specify specific conditions all employ conventional techniques in this technical field or follow the conditions recommended by the manufacturer; reagents or instruments whose manufacturers are not specified are all commercially available conventional products.

[0034] To make the objectives, technical solutions, and advantages of this invention clearer and more explicit, the invention is further described in detail below through embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0035] Example 1

[0036] A traditional Chinese medicine composition for treating gout, comprising the following components: 600g cinnamon twig, 300g sulfur, 200g cyperus rhizome, 200g white peony root, and 100g croton seed. The preparation method of the above-mentioned traditional Chinese medicine composition includes the following specific steps:

[0037] (1) Cinnamon twig, Cyperus rhizome, white peony root, and croton seed were respectively put into a shredder for fragmentation to obtain coarse slices of each raw material with an average diameter of about 1 cm.

[0038] (2) Put the raw materials and sulfur into an automatic mixer and mix for 15 minutes to obtain a premixed medicine. Put the premixed medicine into a pulverizer and pulverize it through an 80-mesh sieve. Then put it into a mixer and mix for 30 minutes to obtain a traditional Chinese medicine composition for treating gout.

[0039] Example 2

[0040] A traditional Chinese medicine composition for treating gout, the composition and preparation method of which are basically the same as those in Example 1, except that the amount of sulfur is 150g.

[0041] Example 3

[0042] A traditional Chinese medicine composition for treating gout, the composition and preparation method of which are basically the same as those in Example 1, except that the amount of cinnamon twig is 300g.

[0043] Comparative Example 1

[0044] A traditional Chinese medicine composition for treating gout, the composition and preparation method of which are basically the same as those in Example 1, except that sulfur is not added.

[0045] Comparative Example 2

[0046] A traditional Chinese medicine composition for treating gout, the composition and preparation method of which are basically the same as those in Example 1, except that cinnamon twigs are not added.

[0047] Comparative Example 3

[0048] A traditional Chinese medicine composition for treating gout, the composition and preparation method of which are basically the same as those in Example 1, except that Cyperus rotundus, Paeonia lactiflora and Croton tiglium are not added.

[0049] To verify the therapeutic effect of the traditional Chinese medicine composition for treating gout on gout, the following in vivo pharmacodynamic tests were conducted.

[0050] Experimental Example 1:

[0051] Virus source: Ten fresh geese that died from gout with a clinical mortality rate >30% were collected. Liver and kidney were collected and ground thoroughly in a mortar with 5 times the volume of sterile physiological saline to prepare a suspension. The suspension was then subjected to three freeze-thaw cycles, centrifuged at 8000 rpm, and the supernatant was collected. The suspension was filtered through a 0.22 μm filter for sterilization. 0.5 mL of the filtrate was inoculated onto each 12-day-old goose embryo and incubated at 38℃. Allantoic fluid from goose embryos that died within 25–168 hours of inoculation was collected. Genetic material was extracted, amplified, and sequenced, confirming the goose astrovirus (named strain KF01) GoAstV2.

[0052] 1. Experimental subjects and grouping

[0053] Sixty-four 4-day-old goslings (purchased from Sunshine Hatchery, tested negative for astrovirus) were randomly divided into eight groups: control group (no challenge, no administration), model group (challenge, no administration), experimental group 1 (challenge, administration - Example 1), experimental group 2 (challenge, administration - Example 2), experimental group 3 (challenge, administration - Example 3), experimental group 4 (challenge, administration - Comparative Example 1), experimental group 5 (challenge, administration - Comparative Example 2), and experimental group 6 (challenge, administration - Comparative Example 2).

[0054] 2. Experimental Design

[0055] Each gosling in the challenge group was inoculated with 0.5 mL of allantoic fluid containing GoAstV2 virus, while each gosling in the non-challenge group was inoculated with 0.5 mL of sterile saline. The challenge group received the drug on the same day, with each gosling receiving 1 g of the drug for 5 consecutive days. Afterward, they were fed normally daily with ample water and feed. The specific experimental protocol is shown in Table 1.

[0056] Table 1 Experimental scheme for Experiment Example 1

[0057]

[0058] 3. Experimental Indicators

[0059] Blood samples were collected from 1, 3, 5, 7, 9, 11, and 13 days after the viral challenge (only from surviving individuals). Serum was collected, and the levels of uric acid and creatinine in the serum were measured using a kit method. The number of deaths and survivors was recorded daily.

[0060] 4. Experimental Results

[0061] Following the viral challenge, some geese exhibited significant depression within 24 hours, and began to die around 72 hours (approximately 3 days), with noticeable excretion of lime-like feces. Mortality occurred in all groups except the control group, and the dead geese showed significant urate deposition in their internal organs, with the most severe deposition in the kidneys. Urate deposition was also observed in the liver, subcutaneous tissue, bile, and joints in some geese. This suggests a preliminary diagnosis of gout in the affected geese, indicating a successful disease model.

[0062] 4.1 Survival Results

[0063] The survival rates of geese in each group after viral challenge at 1d, 3d, 5d, 7d, 9d, 11d, and 13d are shown below. Figure 1 As shown.

[0064] from Figure 1 It can be seen that the control group maintained a 100% survival rate throughout; the model group began to experience mortality from the second day after infection, with the peak mortality occurring between days 3 and 6, ultimately resulting in a 10% survival rate; experimental group 1 experienced mortality within 3 to 5 days, but ultimately maintained an 80% survival rate, with relatively few deaths; the mortality trends of the other experimental groups were generally similar, but while experimental groups 4, 5, and 6 also had higher final survival rates than the model group, they were significantly lower than those of experimental group 1. This indicates that the treatment regimen in experimental group 1 was more effective in controlling the disease.

[0065] 4.2 Serum uric acid results

[0066] The average serum uric acid levels of geese in each group at 1, 3, 5, 7, 9, 11, and 13 days after viral challenge are shown in the figure below. Figure 2 As shown.

[0067] from Figure 2It can be seen that the uric acid level in the blank group remained consistently around 405 μmol / L. In the model group, the uric acid level increased from day 3 of infection, peaked on day 7, and then gradually decreased, returning to near normal by day 13. Experimental group 1 also showed an increase within 3 days, peaking on day 7, and then gradually decreasing, but the increase was significantly smaller than that in the model group, and it returned to near normal levels by day 9. The trends in uric acid changes in the other experimental groups were generally similar to those in experimental group 1, but the magnitude of the increase varied considerably, especially in experimental groups 4, 5, and 6. While the increase levels in these groups were lower than in the model group, they were significantly higher than in experimental groups 1, 2, and 3. This indicates that the drug in experimental group 1 had the best effect on controlling uric acid in this disease model, while the effects in experimental groups 4, 5, and 6 were relatively poor.

[0068] 4.3 Serum creatinine results

[0069] The average serum creatinine levels of geese in each group at 1, 3, 5, 7, 9, 11, and 13 days after viral challenge are shown below. Figure 3 As shown.

[0070] from Figure 3 It can be seen that the creatinine level in the blank group remained around 15 μmol / L throughout; the model group reached a peak of around 100 μmol / L on the third day, and then slowly decreased, returning to normal levels by around the 13th day; the trend of creatinine level changes in each experimental group was basically similar to that of the model group, all reaching a peak on the third day, but the increase in experimental group 1 was significantly smaller, and the increase was lower than that in experimental groups 2 and 3, and also significantly lower than that in experimental groups 4, 5, and 6. This indicates that experimental group 1 had the best creatinine control effect in this disease model.

[0071] From Experiment 1, we can conclude that:

[0072] The virus isolated from the internal organs of geese that died from gout disease can be used to replicate gout. Changes in mortality rate, serum uric acid levels, and serum creatinine levels indicate that the drugs in Examples 1, 2, and 3 have a certain preventive and therapeutic effect on this disease model, and are far superior to the drugs in Comparative Examples 1, 2, and 3. This proves that the proportions of sulfur and cinnamon twigs in the formulation are important and play a crucial role. Formulations lacking sulfur or cinnamon twigs show significantly poorer effects. Furthermore, formulations containing only sulfur and cinnamon twigs also show poor efficacy. This demonstrates that other auxiliary components are also important in the formulation.

[0073] Experimental Example 2:

[0074] 1. Experimental subjects and grouping

[0075] A farmer in Yongfeng County, Jiangxi Province, raised 5,000 Sanhua geese, divided into two flocks: 3,000 in the east flock and 2,000 in the west flock. At 11 days old, dead goslings were found in both flocks simultaneously. Many geese exhibited lethargy and were unable to join the flock. Autopsies of the dead geese revealed obvious characteristics of gout, with white urate deposits in the liver, kidneys, mesenteric serosal surface, gallbladder, and joints. Veterinary diagnosis strongly suggested gout in the geese.

[0076] 2. Experimental Design

[0077] 12 kg of cinnamon twigs, 6 kg of sulfur, 4.5 kg of cyperus rhizome, 4 kg of white peony root, and 4 kg of croton seed were prepared into a powder according to the method in Example 1. To verify the efficacy of the drug, 22.5 kg of sample was provided free of charge for the treatment of the eastern flock. Based on a dosage of 1.5 g per goose per day, the entire flock received 4.5 kg per day for 5 consecutive days. The western flock was treated with a prescription from a local veterinarian, using transfer factor (1.2 mg / kg body weight, orally) + sodium bicarbonate (0.2% mixed with feed) for 5 consecutive days. The specific experimental protocol is shown in Table 2.

[0078] Table 2 Experimental scheme for Experiment Example 2

[0079]

[0080] 3. Experimental Indicators

[0081] Record the number of deaths in two flocks of geese each day and calculate the cumulative mortality rate (on the day medication treatment begins). Mortality rate (%) = cumulative number of deaths (geese) ÷ total number of geese (geese) × 100%.

[0082] 4. Experimental Results

[0083] The mortality rates of the two flocks of geese in Experiment Example 2 are shown in Table 3.

[0084] Table 3 Daily mortality of two flocks of geese after medication in Experiment 2.

[0085]

[0086] Note: During the experiment, due to the high mortality rate in the western group, the farm owner requested that the transfer factor + sodium bicarbonate regimen be discontinued on the 4th day and replaced with the drug from Example 1 for the eastern group.

[0087] As can be seen from Table 3, in the experimental group (sample of this embodiment), the number of daily deaths of swans began to decrease on the second day of treatment, and the mortality was basically controlled after 5 days of treatment. The effect was far better than the transfer factor + sodium bicarbonate regimen.

[0088] From Experiment 2, we can conclude that:

[0089] The medicinal materials used in Example 1 of this invention are used to treat gout in geese that develop naturally, and the therapeutic effect is significant and superior to the conventional treatment methods currently used by some veterinarians.

[0090] Experimental Example 3:

[0091] 1. Experimental subjects and grouping

[0092] A farmer in Leizhou, Zhanjiang, Guangdong Province, raised 3,000 Magang geese, divided into two sheds (groups), each with 1,500 geese. At seven days old, dead goslings were found in both groups simultaneously. When the geese were herded, some sick geese were found unable to join the flock. Autopsy of the dead geese revealed obvious characteristics of gout, with white urate deposits in the liver, kidneys, mesenteric serosal surface, gallbladder, and joints. Veterinary diagnosis strongly suggested gout in the geese.

[0093] 2. Experimental Design

[0094] 4 kg of cinnamon twigs, 2 kg of sulfur, 1 kg of cyperus rhizome, 1 kg of white peony root, and 0.5 kg of croton seed were prepared into a powder according to the method in Example 1. To verify the efficacy of the drug prepared in this invention, 7.5 kg of sample (repackaged into 1 kg bags, 15 bags in total) was provided free of charge for the treatment of the western flock. Based on a dosage of 1 g per goose per day, the entire flock was given 1.5 kg per day for 5 consecutive days. The eastern flock was treated with a prescription from a local veterinarian, using a treatment regimen of goose trait virus yolk antibody (1 mL / goose) + ceftiofur sodium (5 mg / kg body weight) provided by a research institute in Chongqing, administered twice daily. The specific experimental protocol is shown in Table 4.

[0095] Table 4 Experimental scheme for Experiment Example 2

[0096]

[0097] 3. Experimental Indicators

[0098] Record the number of deaths in two flocks of geese each day and calculate the cumulative mortality rate (on the day medication treatment begins). Mortality rate (%) = cumulative number of deaths (geese) ÷ total number of geese (geese) × 100%.

[0099] 4. Experimental Results

[0100] The mortality rates of the two flocks of geese in Experiment 3 are shown in Table 5.

[0101] Table 3. Daily mortality of two flocks of geese after medication in Experiment 3.

[0102]

[0103] As shown in Table 5, in the experimental group (sample of this embodiment), the daily mortality rate of the swan flock began to decrease significantly on the second day after medication, and mortality was basically controlled after 5 days of medication. The control group received antibody treatment, which also showed significant effects; the mortality rate began to decrease on the second day after injection, and mortality was basically controlled by the fifth day after injection. Although the mortality rate was slightly higher than that of the experimental group, this indicates that the therapeutic effect of antibody + ceftiofur sodium is similar to that of the medicinal materials in this embodiment.

[0104] From Experiment 3, we can conclude that:

[0105] The medicinal materials described in Example 1 of this invention have a significant effect on treating naturally occurring gout in geese, and the effect is comparable to, or even slightly better than, the regimen using antibody + ceftiofur sodium injection.

[0106] In summary, this invention effectively overcomes the shortcomings of the prior art and has high industrial application value. The above embodiments are intended to illustrate the substantive content of this invention, but are not intended to limit the scope of protection of this invention. Those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this invention without departing from the essence and scope of protection of this invention.

Claims

1. A traditional Chinese medicine composition for treating gout, characterized in that, The traditional Chinese medicine composition, by weight, consists of: 30-60 parts of cinnamon twig, 15-30 parts of sulfur, 10-20 parts of cyperus rhizome, 10-20 parts of white peony root, and 5-10 parts of croton seed.

2. The method for preparing the traditional Chinese medicine composition for treating gout as described in claim 1, characterized in that, Includes the following steps: (1) Cinnamon twig, Cyperus rhizome, white peony root, and croton seed were shredded separately to obtain coarse slices of each raw material; (2) After mixing the raw materials with sulfur, crush and sieve the mixture to obtain the Chinese medicine composition for treating gout.

3. The method for preparing the traditional Chinese medicine composition for treating gout as described in claim 1, characterized in that, The longest size of the raw material tablets mentioned in step (1) shall not exceed 3cm.

4. The method for preparing the traditional Chinese medicine composition for treating gout as described in claim 1, characterized in that, The sieving in step (2) uses an 80-mesh sieve.

5. A drug for treating gout, characterized in that, The drug is prepared from the traditional Chinese medicine composition for treating gout as described in claim 1 and pharmaceutically acceptable excipients.

6. The medicament for treating gout as described in claim 5, characterized in that, The drug is an oral dosage form.

7. The medicament for treating gout as described in claim 6, characterized in that, The oral dosage form is a powder.

8. The use of the traditional Chinese medicine composition according to claim 1 in the preparation of a medicament for treating gout.

9. The use of the drug according to any one of claims 5-7 in the preparation of a pharmaceutical formulation for treating gout.

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