一种鉴定梨果实硬度的SV标记引物及应用

By developing SV markers and specific primers related to pear fruit firmness, the problem of rapid identification of pear fruit firmness in existing technologies has been solved, enabling rapid identification and breeding guidance during the seedling stage, and improving breeding efficiency and the accuracy of fruit firmness.

CN118755866BActive Publication Date: 2026-07-17NANJING AGRICULTURAL UNIVERSITY

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Filing Date
2024-07-21
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing technologies cannot effectively guide the genetic improvement and breeding of pear fruit firmness, nor can they quickly identify the fruit firmness of different varieties, thus affecting fruit storage and shelf life.

Method used

A molecular marker (SV) closely related to pear fruit firmness was developed. The 286bp SV marker located 855bp upstream of the PyACS1 gene on chromosome 15 of the Red Pear genome was amplified by PCR using specific primers and detected by gel electrophoresis to rapidly identify fruit firmness.

Benefits of technology

This technology enables rapid identification of fruit firmness in different pear varieties during the seedling stage, providing a basis for the breeding and production of new pear varieties and improving the efficiency and accuracy of breeding.

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Abstract

本发明涉及分子标记技术领域,公开了一种鉴定梨果实硬度的SV引物及应用,所述SV标记位于红巴梨基因组第15号染色体的PyACS1基因起始密码子上游855bp位置,是1个286bp的SV标记位点,对于低硬度品种,PyACS1上游855bp处存在该SV序列;对于高硬度品种,PyACS1上游855bp处缺失该SV序列。根据SV标记所在位置开发了特异引物,正向引物序列见SEQ ID NO.2,反向引物序列见SEQ ID NO.3。通过该标记及其引物,可用于梨高低硬度品种的早期快速鉴定。该分子标记可用于梨果实硬度的分子辅助育种选择,为培育稳定遗传的具有更好的商业品质的梨新品种提供新的分子标记。
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