A device for collecting platelet-rich plasma and removing blood fat and a method thereof

Abstract

The application discloses a device and a method for collecting platelet-rich plasma and removing blood fat, which comprises a bottom plate, a blood centrifugal device arranged at the front end of the bottom plate, wherein the blood centrifugal device comprises a centrifugal machine and a centrifugal support matched with the centrifugal machine, the centrifugal support is provided with a blood centrifugal ring, the blood centrifugal ring comprises a first cavity ring, a second cavity ring and a third cavity ring which are detachably connected from bottom to top, and a support assembly is further arranged and provided with a raw blood input assembly on the support assembly. The blood centrifugal device is provided with the centrifugal support, the blood centrifugal ring and the raw blood input assembly arranged on the support assembly, so that the process of continuously outputting the plasma layer, the white blood cell layer, the platelet layer and the red blood cell layer in sequence is realized, and in the continuous collection process, the blood fat separation filter assembly is combined, so that the method for continuously extracting PRP and removing blood fat and returning to the human body at one time is realized.

Description

Technical Field

[0001] This invention relates to the field of blood separation technology, specifically to a device and method for collecting platelet-rich plasma and removing blood lipids. Background Technology

[0002] Blood separation, such as separating individual components from whole blood, typically uses centrifugation to separate various individual components for clinical treatment, scientific research, or raw material preparation. After centrifugation, the blood separates into three layers. The upper layer is plasma, where the lipids that need to be removed are located. The lower layer is the red blood cell layer. The middle layer is the white blood cell and platelet layer (PRP layer). Platelets originate from stem cell differentiation and have special autologous therapeutic effects, such as treating hair loss, musculoskeletal injuries, traumatic soft tissue injuries, joint pain, and chronic trauma. However, current blood separation technologies still have the following drawbacks: First, discontinuous blood separation results in the loss of blood cells and plasma, making it unsuitable for long-term treatment. For example, drawing 50ml of whole blood and centrifuging it to extract PRP (particulate blood plasma), but the PRP layer and red blood cell layer after extraction cannot be reinfused into the body. Secondly, in clinical practice, there is often a need to simultaneously extract PRP and remove lipids from the same patient. Currently, different separation kits and equipment settings are used for PRP extraction and lipid removal, resulting in separate PRP extractions and lipid removal procedures for the same patient. For example, four PRP extractions and four lipid removals would require a total of eight blood purification sessions. Furthermore, a certain time interval is required between each purification session, leading to increased costs, a longer treatment cycle, and increased post-treatment care. Summary of the Invention

[0003] The purpose of this invention is to provide a device and method for collecting platelet-rich plasma (PRP) and removing blood lipids. Through a blood centrifugation device, which includes a centrifugation support, a blood centrifugation ring, and a raw blood input component on the support assembly, a continuous process of sequentially outputting plasma, white blood cells, platelet layer, and red blood cells is achieved. In the continuous collection process, combined with a blood lipid separation and filtration component, a method for one-time continuous extraction of PRP and removal of blood lipids and reinfusion into the human body is realized.

[0004] To achieve the above objective, the present invention provides the following technical solution: an apparatus for collecting platelet-rich plasma and removing blood fat, comprising: a bottom plate, and a blood centrifugation device arranged at the front end of the bottom plate; the blood centrifugation device comprises a centrifuge and a centrifugal support adapted to the centrifuge, wherein a blood centrifugal ring is arranged on the centrifugal support, the blood centrifugal ring comprises a first cavity ring, a second cavity ring and a third cavity ring which are detachably connected from bottom to top, and are respectively used for accommodating a lower red blood cell layer, an intermediate leukocyte and platelet layer and an upper plasma layer generated by centrifugation; the apparatus further comprises a support column assembly, wherein a raw blood input assembly is arranged on the support column assembly, the raw blood input assembly extends into the centrifugal support and is provided with a first connecting pipe assembly, and the first connecting pipe assembly is rotationally connected with the bottom of the blood centrifugal ring and is used for continuously inputting raw blood into the blood centrifugal ring; and a centrifugal channel-changing assembly arranged at the top end of the blood centrifugal ring, wherein the centrifugal channel-changing assembly is communicated with a separated blood output assembly and is used for continuously outputting plasma, the leukocyte and platelet layer and red blood cells in sequence; the apparatus further comprises a main box arranged on the bottom plate, wherein a pipeline connector and a blood fat separation and filtration assembly are arranged on the main box, and are used for further separation of the extracted plasma.

[0005] Preferably, the centrifugal support comprises a centrifugal base, a limiting slot opened on the centrifugal base, a limiting insert block installed on the limiting slot, a support bar fixed on the limiting insert block, and a rotatable slotted hole installed on the centrifugal base; a ring part is fixed at the top of the support bar, and the inner wall of the ring part is fixed to the blood centrifugal ring through a connecting block.

[0006] Preferably, the support column assembly comprises a stand column, a pipe discharging part and a slot base which are respectively arranged at the top and bottom of the stand column, and the slot base can be assembled in the slotted hole on the centrifuge.

[0007] Preferably, the raw blood input assembly comprises a liquid inlet pipe, the liquid inlet pipe is in a "匚"-shape, the upper part of the liquid inlet pipe is fixed to the stand column through an extension plate, and a first rotary sealing pipe sleeve is arranged at the top end of the liquid inlet pipe for connecting a raw blood conduit; the first connecting pipe assembly comprises a connecting pipe and a second rotary sealing pipe sleeve rotationally installed with the connecting pipe, the second rotary sealing pipe sleeve is installed at the bottom end of the liquid inlet pipe; the centers of the first rotary sealing pipe sleeve and the second rotary sealing pipe sleeve coincide with the center of the blood centrifugal ring; and a limiting ring is opened on the inner wall of the centrifugal base, a sliding block is limited and installed in the limiting ring, and the sliding block is fixedly connected with the liquid inlet pipe.

[0008] Preferably, the centrifugal reversing assembly includes an extension cavity disposed at and communicating with the top end of a third chamber ring; a transmission bar disposed at the connection between the extension cavity and the third chamber ring; a second spring connecting the transmission bar and the extension cavity; a limiting plate disposed at the inner end of the third chamber ring; a limiting frame slidably mounted on the limiting plate; an elastic connection portion disposed between the limiting frame and the end of the transmission bar; the transmission bar, the elastic connection portion, and the limiting frame constituting a dynamic discharge cavity; a liquid outlet hole opened at the top of the limiting frame; and a material distribution trough disposed on the third chamber ring; the material distribution trough having a first channel groove, a second channel groove, and a third channel groove inside; the liquid outlet hole communicating sequentially with the first channel groove, the second channel groove, and the third channel groove; the elastic connection portion including a mounting sleeve disposed on the side wall of the limiting frame; a third spring disposed inside the mounting sleeve; and a sliding block slidably mounted inside the mounting sleeve; the end of the transmission bar extending into the mounting sleeve and connected to the sliding block; a pressure sensor disposed on the side wall of the sliding block away from the transmission bar; and the pressure sensor connected to the end of the third spring.

[0009] Preferably, the blood separation output assembly includes a third conduit, a second conduit, and a first conduit, which are connected to the first channel groove, the second channel groove, and the third channel groove in a one-to-one correspondence. The third conduit, the second conduit, and the first conduit have the same structure and are used to export plasma, white blood cells, platelet layer, and red blood cell layer, respectively. The first conduit includes a second catheter, a first catheter, and a third rotary sealing sleeve connected between the second catheter and the first catheter. The center of the third rotary sealing sleeve coincides with the center of the third lumen ring. The second catheter is connected to the third channel groove, and the first catheter passes through the groove where the drain fitting is located and extends for a certain distance.

[0010] Preferably, a first assembly and a second assembly are respectively connected between the first cavity ring and the second cavity ring, and between the second cavity ring and the third cavity ring, for detachable connection of the first cavity ring, the second cavity ring, and the third cavity ring; the first cavity ring and the second cavity ring are respectively connected to a first liquid level regulator and a second liquid level regulator, for moving the lower red blood cell layer, the middle white blood cell and platelet layer, and the upper plasma layer to the corresponding first cavity ring, second cavity ring, and third cavity ring, and performing chamber division under the action of the first assembly and the second assembly.

[0011] Preferably, the first and second assembly components have the same structure. The first assembly component includes a first mounting ring and a second mounting ring, which are respectively disposed on opposite ends of the first and second cavity rings. A wedge ring is provided at the end of the second mounting ring, and a meshing clamping head assembly is provided on the first mounting ring in a ring-like arrangement. The meshing clamping head assembly includes a movable column that is limited to the first mounting ring and can be telescopically moved. A wedge block adapted to the wedge ring is provided at the end of the movable column, and a rack is provided on the movable column. A rotatable internal gear ring is mounted on the first mounting ring, and a ring fixed to the inner wall of the first mounting ring. A rotatable gear is mounted on the ring, and the gear can mesh with the internal gear ring and the rack. A handle block extends from the outer wall of the internal gear ring, and a locking hole is provided on the handle block, along with a locking head assembly fixed to the first mounting ring. The system includes a mounting component on a mounting ring, on which a pin is mounted. A rod cap is fixed to the top of the pin, and a first spring connects the rod cap to the outer wall of the pin between the pin and the mounting component. It also includes a shut-off valve assembly located at the end of a second mounting ring away from the first mounting ring. The shut-off valve assembly includes a mounting plate with several limiting slots. A rotatable transmission ring is located inside the mounting plate. The transmission ring has polygonal grooves, within which baffles are mounted via limiting blocks. Limiting posts are fixed to the baffles and can slide within the limiting slots. A rotating ring on the outer wall of the mounting plate is damped. A connecting rod is located on the inner wall of the rotating ring, passing through a slot in the side wall of the mounting plate and fixedly connected to the transmission ring. A handle is located on the outer wall of the rotating ring, which, when rotated, drives several baffles to open and close.

[0012] Preferably, the first liquid level regulator includes a first extension sleeve disposed at the front end of the first cavity ring, a retractable first push rod mounted on the first extension sleeve, one end of the first push rod extending into the interior of the first cavity ring and disposed of a first piston push plate, the first piston push plate being movable within the flow channel where the first cavity ring is located; the second liquid level regulator structure includes a second extension sleeve communicating with the interior of the second cavity ring, and a retractable second push rod disposed at the end of the second extension sleeve, the second push rod extending into the interior of the second extension sleeve and disposed of a second piston push plate, the second piston push plate being movable within the flow channel where the second cavity ring is located.

[0013] A method for collecting and removing blood lipids from platelet-rich plasma, applied to the aforementioned equipment for collecting and removing blood lipids from platelet-rich plasma, the method comprising: S1: Under the connection of the first connecting tube assembly, the continuous input of the original blood input assembly is not affected. At the same time, the blood is centrifuged at high speed by the centrifuge ring to form layers, namely, the lower red blood cell layer, the middle white blood cell and platelet layer, and the upper plasma layer. S2: Under the action of the centrifugal channel changing component at the top of the blood centrifugal ring, the upper plasma first acts on the centrifugal channel changing component and is located in the innermost layer, corresponding to the inner channel where the blood separation output component is located. Similarly, the white blood cell and platelet layer are connected to the middle channel, and the red blood cell layer is connected to the outer channel, thus realizing the continuous sequential output of plasma, white blood cells, platelet layer and red blood cells. S3: The plasma channel can be connected to the lipid separation and filtration component to realize the process of removing lipids from plasma.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention utilizes a blood centrifugation device comprising a centrifuge support, a blood centrifuge ring, and a raw blood input component mounted on the support assembly. When the centrifuge support drives the blood centrifuge ring to achieve high-speed centrifugation of raw blood, the continuous input of the raw blood input component is not affected by the connection of the first connecting pipe assembly. Simultaneously, the blood undergoes stratification under the blood centrifuge ring, resulting in a lower red blood cell layer, an intermediate white blood cell and platelet layer, and an upper plasma layer. Upon reaching centrifugal sedimentation equilibrium, further, due to centrifugation, the blood centrifuge ring arranges itself from high to low density along its radial circumference towards the axis, consisting of the red blood cell layer, white blood cell and platelet layer, and plasma. Under the action of the centrifuge channel-changing component at the top of the blood centrifuge ring, the upper plasma layer acts first on the centrifuge channel-changing component and is located in the innermost layer, corresponding to the inner channel where the separated blood output component is located. Similarly, the white blood cell and platelet layer connects to the intermediate channel, and the red blood cell layer connects to the outer channel, thus achieving continuous sequential output of plasma, white blood cells, platelets, and red blood cells.

[0015] This invention achieves a method for continuous extraction of PRP and removal of blood lipids in a single process by combining a blood centrifugation device and a blood lipid separation and filtration component, followed by reinfusion into the human body. Furthermore, after extraction, enrichment, and removal of relevant components, the PRP can be reinfused into the human body to achieve therapeutic purposes. This leads to various treatment options, including platelet collection-blood dilution, lipid removal-cell therapy, platelet collection and lipid removal, and blood conversion.

[0016] In another embodiment of the present invention, when the blood centrifuge ring operates independently or discontinuously, the detachable design of the blood centrifuge ring, under the action of the first liquid level regulator and the second liquid level regulator, in conjunction with the action of the shut-off valve assembly, completely isolates the lower red blood cell layer in the first chamber ring, isolates the middle white blood cell and platelet layers in the second chamber ring, and isolates the plasma in the third chamber ring. When the first chamber ring, the second chamber ring, and the third chamber ring are quickly disassembled, the separation liquid in the corresponding chamber can be obtained, solving the problem that it is difficult to extract the separation liquid in existing centrifuge tubes. Attached Figure Description

[0017] Figure 1This is a first-view three-dimensional structural diagram of the present invention; Figure 2 for Figure 1 A second-view 3D structural diagram; Figure 3 for Figure 1 A schematic diagram of the third-person perspective stereoscopic structure; Figure 4 This is an enlarged structural schematic diagram of the blood centrifuge device of the present invention; Figure 5 for Figure 1 A partial disassembly diagram; Figure 6 for Figure 1 A front view structural diagram; Figure 7 This is a schematic diagram of the three-dimensional structure of the blood centrifuge ring of the present invention; Figure 8 This is an enlarged structural diagram of point A in the present invention; Figure 9 This is an enlarged structural diagram of point B in the present invention; Figure 10 This is an enlarged structural diagram of point C in the present invention; Figure 11 This is a partially enlarged structural diagram of the first assembly of the present invention; Figure 12 This is a schematic diagram of the internal structure of the shut-off valve assembly of the present invention; Figure 13 This is a partial cross-sectional structural diagram of the centrifugal lane changer assembly of the present invention; Figure 14 This is an enlarged structural diagram of point D in the present invention; Figure 15 This is an enlarged structural diagram at point E of the present invention; Figure 16 This is a partially enlarged structural diagram of the elastic connection portion of the present invention; Figure 17 This is a three-dimensional structural diagram of the shut-off valve assembly of the present invention; Figure 18 This is a schematic diagram of the three-dimensional structure of the baffle of the present invention; Figure 19 This is a flowchart of the multimodal treatment process of the present invention.

[0018] In the diagram: 111, base plate; 112, centrifuge; 113, main chamber; 114, heating exchange system; 211. Centrifuge base; 212. Limiting ring; 213. Slot; 214. Slider; 216. Support bar; 2161. Limiting block; 217. Ring; 311. Column; 312. Slot base; 314. Pipe fittings; 411. Inlet pipe; 412. First rotary sealing sleeve; 413. Second rotary sealing sleeve; 511. Connecting pipe; 6. Blood centrifuge ring; 611. First chamber ring; 612. First extension sleeve; 613. First push rod; 614. Second chamber ring; 615. Third chamber ring; 620. Second extension sleeve; 621. Second push rod; 631. Transmission bar; 632. Extension cavity; 634. Second spring; 635. Limiting plate; 636. Limiting frame; 637. Liquid outlet; 638. Mounting sleeve; 639. Sliding block; 640. Third spring; 642. Pressure sensor; 643. Distributor trough; 7. Separate blood output assembly; 711. Tubing connector; 712. First tubing; 7121. Third rotary sealing sleeve; 7122. First catheter; 7123. Second catheter; 713. Second tubing; 714. Third tubing; 811. First assembly component; 8111. First mounting ring; 8112. Mounting component; 8113. Handle block; 8114. Rod cap; 8115. Insert post; 8116. Internal gear ring; 8117. First spring; 8118. Gear; 8119. Moving post; 8120. Wedge block; 8122. Ring; 8123. Rack; 820. Handle; 821. Rotary ring; 823. Mounting plate; 8231. Limiting groove; 825. Transmission ring; 8251. Polygonal groove; 826. Baffle; 8261. Limiting post; 8262. Limiting block; 830. Second mounting ring; 831. Wedge ring; 812. Second assembly part; 1012. Lipid separation filter; 1013. Fixture. Detailed Implementation

[0019] In the description of this invention, it should be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships, are based on the orientation or positional relationships shown in the accompanying drawings and are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this invention. The various embodiments of this invention are described in detail below with reference to the accompanying drawings. Example

[0020] Please see Figures 1 to 19The present invention preferably provides a technical solution: a device for collecting platelet-rich plasma and removing blood lipids, comprising: a base plate 111, and a blood centrifugation device disposed at the front end of the base plate 111; the blood centrifugation device includes a centrifuge 112 and a centrifugation support adapted to the centrifuge 112, wherein a blood centrifugation ring 6 is disposed on the centrifugation support, the blood centrifugation ring 6 including a first chamber ring 611, a second chamber ring 614 and a third chamber ring 615 detachably connected from bottom to top, respectively used to accommodate the lower red blood cell layer, the middle white blood cell and platelet layer and the upper plasma layer generated by centrifugation; and further comprising a support assembly, wherein the support assembly is provided with The system includes a raw blood input component that extends into the centrifuge support and is equipped with a first connecting pipe component that is rotatably connected to the bottom of the blood centrifuge ring 6 for continuously inputting raw blood into the blood centrifuge ring 6; a centrifuge channel changer component located at the top of the blood centrifuge ring 6 that is connected to a separated blood output component 7 for continuously and sequentially outputting plasma, white blood cells, platelet layer, and red blood cells; and a main chamber 113 located on a base plate 111, on which a pipeline connector 711 and a blood lipid separation filter component are provided for further separation of extracted plasma.

[0021] In this application, existing methods separate blood into three layers after centrifugation. The upper layer is plasma, where the lipids to be removed are located. The lower layer is the red blood cell layer. The middle layer is the white blood cell and platelet (PRP) layer. Platelets originate from stem cell differentiation and have special autologous therapeutic effects, such as treating hair loss, musculoskeletal injuries, traumatic soft tissue injuries, joint pain, and chronic trauma. Discontinuous blood separation results in the loss of blood cells and plasma, making it unsuitable for long-term treatment. Therefore, a device for continuous collection and lipid removal of platelet-rich plasma was designed. like Figure 1 , 4As shown in Figure 5, the blood centrifugation device includes a centrifuge support, a blood centrifuge ring 6, and a raw blood input component mounted on the support assembly. When the centrifuge support drives the blood centrifuge ring 6 to achieve high-speed centrifugation of raw blood, the continuous input of the raw blood input component is not affected by the connection of the first connecting pipe assembly. Simultaneously, the blood centrifuge ring 6 includes a first chamber ring 611, a second chamber ring 614, and a third chamber ring 615, which are detachably connected from bottom to top. Under high-speed centrifugation, the blood is layered under the blood centrifuge ring 6, resulting in a lower red blood cell layer, a middle layer of white blood cells and platelets, and an upper plasma layer. The blood within is centrifuged by the centrifugal force field. The cells undergo sedimentation and are stratified according to their density, specific gravity, and sedimentation coefficient. When centrifugal sedimentation equilibrium is reached, further, due to centrifugal action, the cells are arranged from high to low density on the radial circumference of the blood centrifuge ring 6 to the axis, in the following order: red blood cell layer, white blood cell and platelet layer, and plasma. Under the action of the centrifugal channel changing component at the top of the blood centrifuge ring 6, the upper plasma layer acts on the centrifugal channel changing component first and is located in the innermost layer, corresponding to the inner channel where the blood separation output component 7 is located. Similarly, the white blood cell and platelet layer are connected to the middle channel, and the red blood cell layer is connected to the outer channel, thus realizing the continuous sequential output of plasma, white blood cell and platelet layer, and red blood cells. Since the aforementioned centrifugation can continuously generate multiple separated blood outputs, the lipid separation and filtration component can further remove blood lipids. This lipid separation and filtration component is a mature existing technology and will not be elaborated upon further. It includes a lipid separation filter 1012, which is mounted on the main chamber 113 via a fixing component 1013. After extraction, enrichment, and removal of the corresponding components, it can be reinfused into the human body to achieve the therapeutic purpose. This leads to various treatment options, such as... Figure 1 , 19 As shown; A series of pipes are installed and connected using pipe connector 711 to form a structure as follows: Figure 19 Treatment tubing; Specifically: One approach: platelet collection - blood dilution, processing of the obtained platelets in tubing, and finally, recirculation and reinfusion back into the patient; The second option is lipid removal and cell rescue; the third option is platelet collection and lipid removal; and the fourth option is further blood conversion.

[0022] Furthermore, the centrifuge support includes a centrifuge seat 211 and a limiting slot opened on the centrifuge seat 211. A limiting block 2161 is installed on the limiting slot. A support bar 216 is fixed on the limiting block 2161, and a rotatable slot 213 is installed on the centrifuge seat 211. A ring portion 217 is fixed to the top of the support bar 216, and the inner wall of the ring portion 217 is fixed to the blood centrifuge ring 6 through a connecting block.

[0023] as shown in Figure 1 and 10 , under the action of the centrifugal seat 211, when it acts on the centrifuge 112, it can be driven to perform high-speed centrifugal rotation. Under the action of the limiting slot on the centrifugal seat 211, when the limiting insertion block 2161 where the support bar 216 is located is inserted downward into the limiting slot, the blood centrifugal ring 6 where the support bar 216 is located is docked downward with the first connecting pipe assembly at the bottom, so as to realize the connection between the blood centrifugal ring 6 and the raw blood input assembly. And under the action of the rotating slot hole 213, the locking of the limiting insertion block 2161 and the limiting slot can be realized.

[0024] Further, the pillar assembly comprises a pillar 311, and a tube discharging member 314 and a slot seat 312 respectively arranged at the top and bottom of the pillar 311, and the slot seat 312 can be assembled in the slot hole 213 on the centrifuge 112; Further, the raw blood input assembly comprises a liquid inlet pipe 411, the liquid inlet pipe 411 is in a shape of "匚", the upper part of the liquid inlet pipe 411 is fixed with the upright post 311 through an extension plate, and a first rotary sealing sleeve 412 arranged at the top end of the liquid inlet pipe 411, which is used for connecting a raw blood conduit; the first connecting pipe assembly comprises a connecting pipe 511 and a second rotary sealing sleeve 413 rotatably mounted with the connecting pipe 511, the second rotary sealing sleeve 413 is mounted at the bottom end of the liquid inlet pipe 411; the centers of the first rotary sealing sleeve 412 and the second rotary sealing sleeve 413 coincide with the center of the blood centrifugal ring 6; and a limiting ring 212 arranged on the inner wall of the centrifugal seat 211, a sliding block 214 is limited and mounted in the limiting ring 212, and the sliding block 214 is fixedly connected with the liquid inlet pipe 411.

[0025] Through the raw blood input assembly arranged on the pillar assembly, as Figure 1 shown, when the slot seat 312 where the upright post 311 is located is inserted into the slot hole 213, the fixed assembly of the upright post 311 can be realized. At this time, the raw blood input assembly completes fixed support through the upright post 311. Under the connecting action of the first rotary sealing sleeve 412 and the second rotary sealing sleeve 413, in cooperation with the fact that the centers of the first rotary sealing sleeve 412 and the second rotary sealing sleeve 413 coincide with the center of the blood centrifugal ring 6, when the blood centrifugal ring 6 rotates at high speed for centrifugation, the raw blood input assembly can normally and continuously input raw blood without affecting the operation of the blood centrifugal ring 6.

[0026] Further, a heat exchange system 114 is arranged on the side wall of the main box 113. Example

[0027] In another embodiment of the present invention, the centrifugal reversing assembly includes an extension cavity 632, which is disposed at and communicates with the top end of a third cavity ring 615; a transmission bar 631 disposed at the connection between the extension cavity 632 and the third cavity ring 615; a second spring 634 connecting the transmission bar 631 and the extension cavity 632; and a limiting plate 635 disposed at the inner end of the third cavity ring 615. A limiting frame 636 is slidably mounted on the limiting plate 635, and an elastic connection is provided between the limiting frame 636 and the end of the transmission bar 631. The transmission bar 631, the elastic connection, and the limiting frame 636 constitute a dynamic discharge cavity. A liquid outlet hole 637 is provided at the top of the limiting frame 636. A material distribution trough 643 is placed on the third cavity ring 615. The material distribution trough 643 is provided with a first channel groove, a second channel groove and a third channel groove inside. The liquid outlet 637 can communicate with the first channel groove, the second channel groove and the third channel groove in sequence. The elastic connection part includes a mounting sleeve 638 provided on the side wall of the limiting frame 636. A third spring 640 is provided inside the mounting sleeve 638. A sliding block 639 is slidably installed inside the mounting sleeve 638. The end of the transmission bar 631 extends into the mounting sleeve 638 and is connected to the sliding block 639. A pressure sensor 642 is provided on the side wall of the sliding block 639 away from the transmission bar 631. The pressure sensor 642 is connected to the end of the third spring 640.

[0028] In this embodiment, a centrifugal diversion component is provided, which utilizes the high-speed centrifugal action of the blood centrifugal ring 6 to perform stratification. The blood inside is subjected to centrifugal force field and undergoes centrifugal sedimentation motion, and is stratified according to its density and sedimentation coefficient. When centrifugal sedimentation equilibrium is reached, a lower red blood cell layer, a middle white blood cell and platelet layer, and an upper plasma layer are obtained. Furthermore, due to centrifugation, the blood is arranged from high density to low density on the radial circumferential surface of the blood centrifugal ring 6 to the axis, in the following order: red blood cell layer, white blood cell and platelet layer, and plasma. Specifically, such as Figure 13 , 14 As shown in Figures 15 and 16, when the upper plasma reaches the centrifugal diverter assembly, it moves the transmission bar 631 towards the extension cavity 632 and compresses the second spring 634 under centrifugal action according to its own density and specific gravity. At this time, the sliding block 639 at the end of the transmission bar 631 moves inside the mounting sleeve 638 and compresses the third spring 640. When plasma is input, and the power output is constant, the transmission bar 631 moves the limiting frame 636 along the limiting plate 635 towards the extension cavity 632 and makes the liquid outlet 637 overlap and connect with the first channel groove on the inner side, which is the plasma extraction channel. Due to the movement of the sliding block 639, the pressure sensor 642 generates a pressure value under the action of the third spring 640, which is the value at which the corresponding plasma extraction channel is triggered. Similarly, when the middle layer of white blood cells and platelets enters the centrifugation channel changing assembly, according to their own density and specific gravity, under centrifugation, the transmission bar 631 further compresses the second spring 634, causing the outlet hole 637 where the limiting frame 636 is located to overlap and connect with the second channel groove, which is the extraction channel for white blood cells and platelets. Similarly, the pressure sensor 642 generates a second pressure value, which is the trigger value corresponding to the extraction channel for white blood cells and platelets. When the lower layer of red blood cells enters the centrifugation channel changing assembly, according to their own density and specific gravity, under centrifugation, the transmission bar 631 moves to its maximum value, causing the outlet hole 637 where the limiting frame 636 is located to overlap and connect with the third channel groove, which is the extraction channel for red blood cells. Similarly, the pressure sensor 642 generates a third pressure value, which is the trigger value corresponding to the extraction channel for red blood cells. Furthermore, the blood separation output assembly 7 includes a third conduit 714, a second conduit 713, and a first conduit 712. The third conduit 714, the second conduit 713, and the first conduit 712 are connected to the first channel groove, the second channel groove, and the third channel groove in a one-to-one correspondence. The third conduit 714, the second conduit 713, and the first conduit 712 have the same structure and are used to export plasma, white blood cells, platelet layer, and red blood cell layer, respectively. The first conduit 712 includes a second catheter 7123, a first catheter 7122, and a third rotary sealing sleeve 7121 connecting the second catheter 7123 and the first catheter 7122. The center of the third rotary sealing sleeve 7121 coincides with the center of the third cavity ring 615. The second catheter 7123 is connected to the third channel groove. The first catheter 7122 passes through the groove where the drain pipe 314 is located and extends for a certain distance.

[0029] Through further configuration of the blood separation output component 7, such as Figure 1 , 4 As shown in Figures 5 and 15, through the further configured third conduit 714, second conduit 713, and first conduit 712, and under the action of their connecting pipes, due to the third rotary sealing sleeve 7121 set between the second conduit 7123 and the first conduit 7122, and the center of the third rotary sealing sleeve 7121 coinciding with the center of the third cavity ring 615, when the blood centrifuge ring 6 rotates at high speed, the third rotary sealing sleeve 7121 is limited and fixed by the pipe fitting 314 where the column 311 is located. Under the action of the third rotary sealing sleeve 7121, the second conduit 7123 can follow the blood centrifuge ring 6 to rotate at high speed, thus realizing the continuous collection process of plasma, white blood cells, platelet layer, and red blood cell layer. Example

[0030] As another embodiment of the present invention, a first fitting 811 and a second fitting 812 are respectively connected between the first cavity ring 611 and the second cavity ring 614, and between the second cavity ring 614 and the third cavity ring 615, for detachable connection of the first cavity ring 611, the second cavity ring 614 and the third cavity ring 615; the first cavity ring 611 and the second cavity ring 614 are respectively connected to a first liquid level regulator and a second liquid level regulator, for moving the lower red blood cell layer, the middle white blood cell and platelet layer and the upper plasma layer to the corresponding first cavity ring 611, second cavity ring 614 and third cavity ring 615, and performing chamber division under the action of the first fitting 811 and the second fitting 812.

[0031] Furthermore, the first assembly 811 and the second assembly 812 have the same structure. The first assembly 811 includes a first mounting ring 8111 and a second mounting ring 830, which are respectively disposed at the opposite ends of the first cavity ring 611 and the second cavity ring 614. A wedge ring 831 is provided at the end of the second mounting ring 830, and an engagement clamping head assembly is provided on the first mounting ring 8111 in a ring-shaped arrangement. The engagement clamping head assembly includes a movable column 8119 that is limited to and retractably movable on the first mounting ring 8111. The end of the 8119 is provided with a wedge block 8120 adapted to the wedge ring 831, and a rack 8123 is provided on the movable column 8119. A rotatable internal gear ring 8116 is installed on the first mounting ring 8111, and a ring 8122 is fixed on the inner wall of the first mounting ring 8111. A rotatable gear 8118 is installed on the ring 8122, and the gear 8118 can mesh with the internal gear ring 8116 and the rack 8123. A handle block 8113 extends from the outer wall of the internal gear ring 8116. The handle block 8113 is provided with a locking hole and is fixed to the first mounting ring 8111. Mounting component 8112 on mounting ring 111 has a pin 8115 mounted on it. A rod cap 8114 is fixed to the top of the pin 8115. A first spring 8117 is connected to the outer wall of the pin 8115 between the rod cap 8114 and the mounting component 8112. The system also includes a shut-off valve assembly located at the end of the second mounting ring 830 away from the first mounting ring 8111. The shut-off valve assembly includes a mounting plate 823 with several limiting slots 8231. A rotatable transmission ring 825 is located inside the mounting plate 823. The transmission ring 825 has... The polygonal groove 8251 has a baffle 826 installed inside it via a limiting block 8262, and a limiting post 8261 fixed on the baffle 826. The limiting post 8261 can slide within the limiting long groove 8231 and dampen the rotation of the rotating ring 821 on the outer wall of the mounting plate 823. The inner wall of the rotating ring 821 is provided with a connecting rod, which passes through the through groove on the side wall of the mounting plate 823 and is fixedly connected to the transmission ring 825. A handle 820 is provided on the outer wall of the rotating ring 821. When the handle 820 is rotated, it is used to drive the opening and closing movement of several baffles 826.

[0032] Furthermore, the first liquid level regulator includes a first extension sleeve 612 disposed at the front end of the first cavity ring 611, a retractable first push rod 613 mounted on the first extension sleeve 612, one end of the first push rod 613 extending into the interior of the first cavity ring 611 and provided with a first piston push plate, the first piston push plate being movable within the flow channel where the first cavity ring 611 is located; the second liquid level regulator structure includes a second extension sleeve 620, the second extension sleeve 620 communicating with the interior of the second cavity ring 614, and a retractable second push rod 621 disposed at the end of the second extension sleeve 620, the second push rod 621 extending into the interior of the second extension sleeve 620 and provided with a second piston push plate, the second piston push plate being movable within the flow channel where the second cavity ring 614 is located.

[0033] In this embodiment, since the blood centrifugation ring 6 includes a first chamber ring 611, a second chamber ring 614 and a third chamber ring 615 that are detachably connected from bottom to top, respectively for accommodating the lower red blood cell layer, the middle white blood cell and platelet layer and the upper plasma layer generated by centrifugation, it can be sorted and collected during the continuous blood separation and collection process. The detachable design of the blood centrifuge ring 6 allows for the rapid separation of red blood cell layer, white blood cell and platelet layer, and plasma even in discontinuous blood separation processes. During use, such as Figure 5 , 7 As shown in Figures 8, 9, and 11, the first fitting 811 and the second fitting 812 provided between the first cavity ring 611 and the second cavity ring 614, and between the second cavity ring 614 and the third cavity ring 615, are used for the detachable connection of the first cavity ring 611, the second cavity ring 614, and the third cavity ring 615. Specifically, regarding the structural configuration of the first assembly 811, when the first mounting ring 8111 where the first cavity ring 611 is located and the second mounting ring 830 where the second cavity ring 614 is located are connected, the manual drive handle block 8113 drives the internal gear ring 8116 to rotate. Under the meshing transmission action of the internal gear ring 8116, gear 8118, and rack 8123, several movable columns 8119 can be driven to move closer to the axis, thereby causing the wedge block 8120 to act on the wedge ring 831, so that the wedge ring 831 and the end face of the first mounting ring 8111 are further squeezed under the action of the inclined plane. Preferably, both end faces are provided with sealing rings, which can realize the tight assembly of the first cavity ring 611 and the second cavity ring 614, improve the sealing of the connection position, and avoid leakage. Under the action of the first level regulator and the second level regulator, such as Figure 7As shown, the blood centrifuge ring 6 includes a first chamber ring 611, a second chamber ring 614, and a third chamber ring 615, which are detachably connected from bottom to top. All three are made of transparent material. After centrifugation and stratification, the layers are, in sequence, a lower red blood cell layer, a middle layer of white blood cells and platelets, and an upper layer of plasma. Moving the first liquid level regulator allows the first piston pusher to move towards direction 811 in the flow channel where the first chamber ring 611 is located, until the boundary between the red blood cell layer and the middle layer of white blood cells and platelets is positioned at the cut-off valve assembly where 811 is located. At this point, the cut-off valve assembly closes, completely isolating the lower red blood cell layer within the first chamber ring 611. Similarly, when the first level regulator is moved... When the second level regulator is activated, the second piston pusher plate is pushed to move towards the third chamber ring 615 inside the flow channel where the second chamber ring 614 is located, until the boundary between the intermediate layer of white blood cells, platelets and plasma is moved to the position of the shut-off valve assembly where the second assembly 812 is located. When the shut-off valve assembly at this position is closed, the intermediate layer of white blood cells and platelets is isolated in the second chamber ring 614 and the plasma is isolated in the third chamber ring 615. When the first chamber ring 611, the second chamber ring 614 and the third chamber ring 615 are quickly disassembled, the separation liquid in the corresponding chamber can be obtained, which solves the problem that it is difficult to extract the separation liquid in existing centrifuge tubes. This invention provides an embodiment of a shut-off valve assembly, such as... Figure 12 , 17 As shown in Figure 18, when the manual drive handle 820 is activated, the rotating ring 821 can rotate relative to the mounting plate 823, which in turn drives the transmission ring 825 to rotate. Under the limiting action of the polygonal groove 8251 and the limiting block 8262, and with the further limiting action of the limiting post 8261 and the limiting long groove 8231, since the mounting plate 823 is fixed relative to the transmission ring 825, when the transmission ring 825 rotates, it can drive several baffles 826 to open and close. When they are open, they connect the flow channels of the first chamber ring 611, the second chamber ring 614 and the third chamber ring 615. When they are closed, they can divide the first chamber ring 611, the second chamber ring 614 and the third chamber ring 615 into independent chambers so as to extract the corresponding separation liquid.

[0034] A method for collecting and removing blood lipids from platelet-rich plasma, applied to the aforementioned equipment for collecting and removing blood lipids from platelet-rich plasma, the method comprising: S1: Under the connection of the first connecting tube assembly, the continuous input of the original blood input assembly is not affected. At the same time, the blood centrifugation ring 6 is centrifuged at high speed to separate the blood into layers, namely, the lower red blood cell layer, the middle white blood cell and platelet layer, and the upper plasma layer. S2: Under the action of the centrifugal channel changing component at the top of the blood centrifugal ring 6, the upper plasma first acts on the centrifugal channel changing component and is located in the innermost layer, corresponding to the inner channel where the blood separation output component 7 is located. Similarly, the white blood cell and platelet layer are connected to the middle channel, and the red blood cell layer is connected to the outer channel, thus realizing the continuous sequential output of plasma, white blood cells, platelet layer and red blood cells. S3: The plasma channel can be connected to the lipid separation and filtration component to realize the process of removing lipids from plasma.

[0035] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can also refer to a mechanical connection. Detachable installation can take many forms, such as through a combination of plug-in and snap-fit ​​connections, or through bolted connections, etc.

[0036] The above embodiments, which describe the specific features of the present invention, are only used to further illustrate the present invention and should not be construed as limiting the scope of protection of the present invention. Any non-essential improvements and adjustments made to the present invention by those skilled in the art based on the above description of the invention shall fall within the scope of protection of the present invention.

Claims

1. A device for collecting platelet-rich plasma and removing blood lipids, characterized in that, include: A base plate (111), and a blood centrifugation device disposed at the front end of the base plate (111); The blood centrifugation device includes a centrifuge (112) and a centrifuge support adapted to the centrifuge (112). The centrifuge support is provided with a blood centrifugation ring (6). The blood centrifugation ring (6) includes a first chamber ring (611), a second chamber ring (614), and a third chamber ring (615) that are detachably connected from bottom to top, respectively for accommodating the lower red blood cell layer, the middle white blood cell and platelet layer, and the upper plasma layer generated by centrifugation. It also includes a support assembly, on which a raw blood input assembly is provided. The raw blood input assembly extends into the centrifuge support and is provided with a first connecting pipe assembly. The first connecting pipe assembly is rotatably connected to the bottom of the blood centrifuge ring (6) for continuously inputting raw blood into the blood centrifuge ring (6). And a centrifugal diversion assembly disposed at the top of the blood centrifugal ring (6), the centrifugal diversion assembly being connected to a blood separation output assembly (7) for continuously and sequentially outputting plasma, white blood cells, platelet layer and red blood cells; The centrifugal reversing assembly includes an extension cavity (632) disposed at and communicating with the top end of a third cavity ring (615), and a transmission bar (631) disposed at the connection between the extension cavity (632) and the third cavity ring (615), wherein a second spring (634) is connected between the transmission bar (631) and the extension cavity (632); And a limiting plate (635) is provided at the inner end of the third cavity ring (615). The limiting plate (635) is slidably mounted with a limiting frame (636). An elastic connection is provided between the limiting frame (636) and the end of the transmission bar (631). The transmission bar (631), the elastic connection, and the limiting frame (636) constitute a dynamic discharge cavity. A liquid outlet hole (637) is provided at the top of the limiting frame (636). And a material distribution trough (643) is provided on the third cavity ring (615). The material distribution trough (643) is provided with a first channel groove, a second channel groove, and a third channel groove. The liquid outlet hole (637) can be connected to the first channel groove, the second channel groove, and the third channel groove in sequence. The elastic connection includes a mounting sleeve (638) disposed on the side wall of the limiting frame (636), a third spring (640) disposed inside the mounting sleeve (638), and a sliding block (639) slidably mounted inside the mounting sleeve (638). The end of the transmission bar (631) extends into the mounting sleeve (638) and is connected to the sliding block (639). A pressure sensor (642) is disposed on the side wall of the sliding block (639) away from the transmission bar (631). The pressure sensor (642) is connected to the end of the third spring (640). It also includes a main box (113) set on the base plate (111), on which a pipeline connector (711) and a blood lipid separation filter assembly are provided for further separation of extracted plasma.

2. The device for collecting platelet-rich plasma and removing blood lipids according to claim 1, characterized in that: The centrifugal support includes a centrifugal seat (211), a limiting slot opened on the centrifugal seat (211), a limiting insert (2161) installed on the limiting slot, a support bar (216) fixed on the limiting insert (2161), and a rotatable slotted hole (213) installed on the centrifugal seat (211); a ring portion (217) is fixed on the top of the support bar (216), and the inner wall of the ring portion (217) is fixed to the blood centrifugal ring (6) via a connecting block.

3. The device for collecting platelet-rich plasma and removing blood lipids according to claim 1, characterized in that: The strut assembly includes a vertical column (311), and a tube discharging member (314) and a slot seat (312) respectively arranged at the top and bottom of the vertical column (311), wherein the slot seat (312) can be fitted in the slotted hole (213) on the centrifuge (112).

4. The device for collecting platelet-rich plasma and removing blood lipids according to claim 1, characterized in that: The raw blood input assembly includes a liquid inlet tube (411), the liquid inlet tube (411) is in a "匚"-shape, the upper part of the liquid inlet tube (411) is fixed to the vertical column (311) via an extension plate, and a first rotary sealing sleeve (412) arranged at the top end of the liquid inlet tube (411) for connecting a raw blood catheter; The first connecting tube assembly includes a connecting tube (511), and a second rotary sealing sleeve (413) rotatably installed with the connecting tube (511), the second rotary sealing sleeve (413) is installed at the bottom end of the liquid inlet tube (411); the centers of the first rotary sealing sleeve (412) and the second rotary sealing sleeve (413) coincide with the center of the blood centrifugal ring (6); and a limiting ring (212) opened on the inner wall of the centrifugal seat (211), a sliding block (214) is limited and installed in the limiting ring (212), and the sliding block (214) is fixedly connected with the liquid inlet tube (411).

5. The device for collecting platelet-rich plasma and removing blood lipids according to claim 1, characterized in that: The separated blood output assembly (7) includes a third pipeline (714), a second pipeline (713) and a first pipeline (712), the third pipeline (714), the second pipeline (713) and the first pipeline (712) are in one-to-one corresponding communication with the first channel slot, the second channel slot and the third channel slot; The third pipeline (714), the second pipeline (713) and the first pipeline (712) have the same structure, and are respectively used to导出 plasma, the layer of white blood cells and platelets, and the red blood cell layer. The first pipeline (712) includes a second catheter (7123), a first catheter (7122), and a third rotary sealing sleeve (7121) connected between the second catheter (7123) and the first catheter (7122). The center of the third rotary sealing sleeve (7121) coincides with the center of the third cavity ring (615), the second catheter (7123) communicates with the third channel slot, and the first catheter (7122) passes through the groove where the tube discharging member (314) is located and extends for a certain distance.

6. The device for collecting platelet-rich plasma and removing blood lipids according to claim 1, characterized in that: The first fitting (811) and the second fitting (812) are respectively connected between the first cavity ring (611) and the second cavity ring (614), and between the second cavity ring (614) and the third cavity ring (615), for detachable connection of the first cavity ring (611), the second cavity ring (614) and the third cavity ring (615); the first cavity ring (611) and the second cavity ring (614) are respectively connected to the first liquid level regulator and the second liquid level regulator, for moving the lower red blood cell layer, the middle white blood cell and platelet layer and the upper plasma layer to the corresponding first cavity ring (611), the second cavity ring (614) and the third cavity ring (615), and performing chamber division under the action of the first fitting (811) and the second fitting (812).

7. The device for collecting and removing blood lipids from platelet-rich plasma according to claim 6, characterized in that: The first assembly (811) and the second assembly (812) have the same structure. The first assembly (811) includes a first mounting ring (8111) and a second mounting ring (830). The first mounting ring (8111) and the second mounting ring (830) are respectively disposed on the opposite ends of the first cavity ring (611) and the second cavity ring (614). The end of the second mounting ring (830) is provided with a wedge ring (831), and a meshing clamping head assembly is disposed on the first mounting ring (8111) in a ring-shaped distribution. The engagement clamping head assembly includes a retractable movable column (8119) limited on a first mounting ring (8111), the end of the movable column (8119) being provided with a wedge (8120) adapted to a wedge ring (831), and a rack (8123) disposed on the movable column (8119). A rotatable internal gear ring (8116) is mounted on the first mounting ring (8111), and an annular ring (8122) fixed on the inner wall of the first mounting ring (8111). A rotatable gear (8118) is mounted on the annular ring (8122). The wheel (8118) can mesh with the internal gear ring (8116) and the rack (8123); the outer wall of the internal gear ring (8116) extends a handle block (8113), the handle block (8113) is provided with a locking hole, and a mounting member (8112) is fixed on the first mounting ring (8111), a plug (8115) is installed on the mounting member (8112), a rod cap (8114) is fixed on the top of the plug (8115), and a first spring (8117) is connected to the outer wall of the plug (8115) between the rod cap (8114) and the mounting member (8112). It also includes a shut-off valve assembly disposed at the end of the second mounting ring (830) away from the first mounting ring (8111). The shut-off valve assembly includes a mounting plate (823), on which a plurality of limiting slots (8231) are formed. A rotatable transmission ring (825) is disposed inside the mounting plate (823). A polygonal groove (8251) is formed on the transmission ring (8251). A baffle (826) is installed in the polygonal groove (8251) by means of a limiting block (8262), and a baffle (826) is fixed to the baffle (8262). 26) The limiting post (8261) on the mounting plate (823) can slide within the limiting groove (8231) and the rotating ring (821) on the outer wall of the mounting plate (823) is damped and rotated. The inner wall of the rotating ring (821) is provided with a connecting rod, which passes through the through groove of the side wall of the mounting plate (823) and is fixedly connected to the transmission ring (825). The handle (820) on the outer wall of the rotating ring (821) is provided. When the handle (820) rotates, it is used to drive several baffles (826) to open and close.

8. The device for collecting platelet-rich plasma and removing blood lipids according to claim 6, characterized in that: The first liquid level regulator includes a first extension sleeve (612) disposed at the front end of the first cavity ring (611), and a retractable first push rod (613) is mounted on the first extension sleeve (612). One end of the first push rod (613) extends into the interior of the first cavity ring (611) and is provided with a first piston push plate. The first piston push plate can move inside the flow channel where the first cavity ring (611) is located. The second liquid level regulator structure includes a second extension sleeve (620), which is connected to the interior of the second cavity ring (614), and the end of the second extension sleeve (620) is provided with a retractable second push rod (621). The second push rod (621) extends into the interior of the second extension sleeve (620) and is provided with a second piston push plate. The second piston push plate can move inside the flow channel where the second cavity ring (614) is located.

9. A method for collecting and removing blood lipids from platelet-rich plasma, applied to the apparatus for collecting and removing blood lipids from platelet-rich plasma as described in any one of claims 1 to 8, characterized in that: The method includes: S1: Under the connection of the first connecting tube assembly, the continuous input of the original blood input assembly is not affected. At the same time, the blood centrifugation ring (6) is centrifuged at high speed to separate the layers, that is, the lower red blood cell layer, the middle white blood cell and platelet layer and the upper plasma layer are obtained. S2: Under the action of the centrifugal channel change component at the top of the blood centrifugal ring (6), the upper plasma first acts on the centrifugal channel change component and is located in the innermost layer, corresponding to the inner channel where the blood separation output component (7) is located. Similarly, the white blood cell and platelet layer are connected to the middle channel, and the red blood cell layer is connected to the outer channel, thus realizing the continuous sequential output of plasma, white blood cells, platelet layer and red blood cells. S3: The plasma channel can be connected to the lipid separation and filtration component to realize the process of removing lipids from plasma.

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