彩色油菜的内参基因及其引物和应用
By screening and applying the EF-1α gene as an internal reference gene, the problem of unstable internal reference genes in existing technologies has been solved, and the accuracy and reliability of gene expression analysis in the petals, leaves and stems of colored rapeseed have been improved, thus enhancing the reliability of qRT-PCR results.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- ZHEJIANG UNIV
- Filing Date
- 2024-08-29
- Publication Date
- 2026-07-17
AI Technical Summary
The lack of internal reference genes that can be stably expressed under different treatment conditions, at all growth and development stages, and in different tissues in the current technology affects the accuracy of qRT-PCR results and makes it difficult to effectively correct gene expression analysis results.
The EF-1α gene suitable for colored rapeseed petals, leaves and stem tissues was screened as an internal reference gene, and corresponding primers were designed. Gene expression analysis was performed by real-time quantitative PCR. The stability of candidate internal reference genes was evaluated using RefFinder software, and the EF-1α gene was determined to be the best internal reference gene.
It improves the reliability and accuracy of qRT-PCR results, provides an effective correction tool, and ensures the accuracy of studies on differentially expressed genes in the pigment metabolism pathway of rapeseed.
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Figure CN118813862B_ABST