A nanoemulsion eye cream containing traditional Chinese medicine compound extract and a preparation method thereof

Abstract

The present application relates to the skin care technical field, specifically, a kind of nanometer emulsion eye cream containing traditional Chinese medicine compound extract and preparation method thereof.The traditional Chinese medicine compound extract is the alcohol extract of the following weight parts of raw materials: 20-35 parts of dogwood, 10-20 parts of forsythia viridissima leaf, 10-20 parts of panax notoginseng, 10-15 parts of schisandra chinensis, 25-30 parts of cassia seed and 5-15 parts of dendrobium nobile.The preparation method comprises the following steps: S1. dogwood, forsythia viridissima leaf, panax notoginseng, schisandra chinensis, cassia seed and dendrobium nobile are uniformly mixed, baked, crushed, sieved to obtain traditional Chinese medicine powder;S2. the traditional Chinese medicine powder is added into ethanol solution for ultrasonic extraction, then decolorized, concentrated to ethanol volatilization, repeated extraction 3 times, the concentrated solution is combined and dried, and the traditional Chinese medicine compound extract is obtained.The various medicines used for preparing the traditional Chinese medicine compound extract are all included in "dual-purpose list of food and medicine", the product has stable performance, is mild and non-irritating, has good safety, significant curative effect, small side effect and no harm to skin.

Description

Technical Field

[0001] This invention relates to the field of skincare technology, specifically to a nano-emulsion eye cream containing extracts of traditional Chinese medicine compound and its preparation method. Background Technology

[0002] Conjunctivitis is a common ophthalmic disease, especially acute bacterial conjunctivitis, which accounts for as much as 20.19% of ophthalmology outpatient visits. It is highly contagious and seasonal, occurring most frequently in summer and autumn. The main symptoms include a foreign body sensation, burning sensation, and itchy eyes; severe cases may also experience tearing, pain, and photophobia. Although the course of the disease is about two weeks, prolonged illness can lead to corneal inflammation and vision damage. Clinical etiological analysis shows that the main pathogens of bacterial conjunctivitis are Staphylococcus epidermidis, Staphylococcus aureus, and Escherichia coli. Acute bacterial conjunctivitis is generally treated with antibiotic ointments and eye drops. However, long-term use of antibiotics has a certain toxicity to corneal and conjunctival epithelial cells, can disrupt the local bacterial flora balance, affect tear film stability, causing dry eyes, and even more serious complications. Furthermore, frequent eye drops affect the adhesion function of tear film mucin to the ocular surface epithelium, reducing tear film stability and subsequently causing burning, dryness, and other discomfort in the eyes, seriously affecting the patient's work and life.

[0003] Nanoemulsion transdermal drug delivery systems offer unique advantages. They can act directly on the administration site, avoiding the first-pass effect of the liver and gastrointestinal side effects, reducing adverse reactions, and are convenient to use. In transdermal drug delivery systems, the efficiency of drug absorption through the skin is a major issue that urgently needs to be addressed. The main factor affecting drug absorption is the barrier function of the stratum corneum. Therefore, how to promote drug penetration through the stratum corneum for transdermal absorption is a key research focus for this type of formulation. Nanoemulsions are emulsions with particle sizes ranging from 10 to 200 nm. Compared to conventional emulsions, nanoemulsions are smaller in size, have better dispersibility, and exhibit better bioavailability, allowing active ingredients to penetrate from the stratum corneum to deeper layers of the skin to exert their effects.

[0004] Eye cream is a type of skincare cosmetic product that moisturizes and firms the skin around the eyes. Besides alleviating dark circles and eye bags, it also helps improve wrinkles and fine lines. There are many types of eye creams, including moisturizing eye creams, firming eye creams, hydrating eye creams, anti-aging eye creams, and anti-allergy eye creams. In recent years, to meet market demand, various functional eye creams have been developed and put into production. However, most current eye creams overemphasize moisturizing and reducing dryness, fine lines, and crow's feet around the eyes, resulting in relatively traditional and fixed product functions. The development of preventative and health-promoting eye creams is still in its early stages. Although natural products are widely used, existing eye creams often have significant side effects, unreasonable ingredient combinations, simple processing methods, and low efficiency in the conversion and delivery of active ingredients. Summary of the Invention

[0005] To address the problems and shortcomings of existing technologies, this invention provides a nano-emulsion eye cream for preventing bacterial conjunctivitis and its preparation method.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] In a first aspect, the present invention provides a compound extract of traditional Chinese medicine, which is an alcohol extract of the following raw materials in parts by weight: 20-35 parts of Cornus officinalis, 10-20 parts of Forsythia suspensa leaf, 10-20 parts of Panax notoginseng, 10-15 parts of Schisandra chinensis, 25-30 parts of Cassia tora seed, and 5-15 parts of Dendrobium nobile.

[0008] Furthermore, the extract of the traditional Chinese medicine compound contains mononoside at a content of 30-60 mg / g, loganin at a content of 5-20 mg / g, forsythoside A at a content of 4-20 mg / g, forsythoside at a content of 1-5 mg / g, notoginsenoside at a content of 5-15 mg / g, rhein at a content of 3-20 mg / g, chlorogenic acid at a content of 6-25 mg / g, and romaine at a content of 2-10 mg / g.

[0009] Secondly, the present invention provides a method for preparing a compound extract of traditional Chinese medicine, comprising the following steps:

[0010] S1. Mix Cornus officinalis, Forsythia suspensa leaves, Panax notoginseng, Schisandra chinensis, Cassia tora seeds, and Dendrobium nobile, bake, pulverize, and sieve to obtain Chinese medicine powder;

[0011] S2. Add the Chinese herbal powder to an ethanol solution for ultrasonic extraction, then decolorize and concentrate until the ethanol evaporates. Repeat the extraction three times, combine the concentrates, and dry to obtain the compound extract of Chinese herbal medicine.

[0012] Preferably, the volume concentration of the ethanol solution in step S2 is 60% to 80%, and the weight-to-volume ratio of the Chinese medicine powder to the ethanol solution is 1g:(30 to 60)mL.

[0013] Preferably, the ultrasonic extraction temperature in step S2 is 60-80℃, and the time is 2-4 hours.

[0014] Preferably, the decolorization in step S2 uses activated carbon, the decolorization pH value is 6-8, and the amount of activated carbon used is 0.5%-3% of the mass of the Chinese medicine powder.

[0015] Preferably, the baking temperature in step S1 is 60-70°C and the baking time is 12-16 hours.

[0016] Preferably, the sieving in step S1 uses a 40-mesh sieve.

[0017] Preferably, the drying temperature in step S2 is 60–70°C.

[0018] Thirdly, the present invention provides the application of the traditional Chinese medicine compound extract described in the first aspect in the preparation of eye cream.

[0019] Fourthly, the present invention provides a nano-emulsion eye cream containing a compound extract of traditional Chinese medicine, which, by weight percentage, comprises the following components: 2-8% of the compound extract of traditional Chinese medicine described in the first aspect, 0.5-2% of rose essential oil, 5-13% of isopropyl myristate, 4-12% of Cornus officinalis seed oil, 1.5-3% of vitamin E, 1-3% of squalane, 0.05-2% of sodium hyaluronate, 3-7% of glycerin, 0.4-0.8% of niacinamide, 1-3% of aloe vera gel, 1-3% of carrageenan, 2-8% of lecithin, 1-5% of polysorbate-80, 0.3-0.8% of menthol, with the balance being water.

[0020] Fifthly, the present invention provides a method for preparing a nano-emulsion eye cream containing extracts of traditional Chinese medicine compound, comprising the following steps:

[0021] (1) Dissolve the compound extract of traditional Chinese medicine in acetone, then add distilled water with a pH of 8-13 and heat until the acetone evaporates to obtain a suspension of traditional Chinese medicine; mix isopropyl myristate, cornus seed oil, rose essential oil, vitamin E, squalane, lecithin, glycerin and polysorbate-80 thoroughly to obtain an oil phase solution;

[0022] (2) The traditional Chinese medicine suspension obtained in step (1) is mixed with the oil phase solution and then homogenized to obtain a nanoemulsion;

[0023] (3) Sodium hyaluronate, nicotinamide, aloe vera gel, carrageenan and menthol are fully dissolved in water at 30-50℃ to obtain a mixed solution. The nanoemulsion obtained in step (2) is added to the mixed solution and mixed evenly. Vacuum sterilization is performed to obtain a nanoemulsion eye cream made from the compound extract of traditional Chinese medicine for the prevention of bacterial conjunctivitis.

[0024] Preferably, the ratio of the traditional Chinese medicine compound extract to acetone in step (1) is (15-30) mg: 1 mL.

[0025] Preferably, the homogenization pressure in step (2) is 250-350 bar, and the number of times is 20-30.

[0026] Preferably, the rose essential oil in step (1) is prepared by three-stage molecular distillation technology, and the rose essential oil recovery rate reaches 95.47%.

[0027] Furthermore, the technical conditions for the three-stage molecular distillation are as follows: preheating temperature 40-60℃, condenser temperature 5-10℃, and feed flow rate 10-15mL / min;

[0028] Furthermore, the technical parameters for the three-stage molecular distillation are as follows: First-stage parameters: evaporation temperature 50–60℃, vacuum degree 35–50 hPa, scraper rotation speed 300–400 r / min; Second-stage parameters: evaporation temperature 70–80℃, vacuum degree 0.35–0.50 hPa, scraper rotation speed 300–400 r / min; Third-stage parameters: evaporation temperature 50–60℃, vacuum degree 35–50 hPa, scraper rotation speed 300–400 r / min.

[0029] Preferably, the Cornus officinalis seed oil in step (1) is prepared by drying Cornus officinalis seeds at low temperature for 8-12 hours, pulverizing them, passing them through a 40-mesh sieve, and then extracting them using supercritical CO2 technology.

[0030] Furthermore, the supercritical CO2 technology conditions are: pressure 350-550 Bar, temperature 45-65℃, and time 2-5h.

[0031] The traditional Chinese medicine compound extract provided by this invention has the following effects for each of the medicinal ingredients:

[0032] Cornus officinalis is one of the major traditional Chinese medicinal herbs, belonging to the category of dual-use medicinal and edible resources. It is rich in flavonoids, iridoids, tannins, and other functional components, possessing a wide range of biological activities, such as antibacterial, anti-inflammatory, antioxidant, anti-aging, and whitening effects. It can be used to prepare cosmetics with functions such as acne prevention and treatment, freckle removal and whitening, anti-skin aging, and wrinkle prevention. Its extract contains abundant antioxidants, which can effectively inhibit tyrosinase activity, preventing excessive melanin production at its source, thereby lightening dark circles and age spots. Furthermore, Cornus officinalis extract has significant antibacterial effects. Zhao Shuyan et al., using Staphylococcus aureus and Escherichia coli as indicator bacteria, studied the antibacterial effect of Cornus officinalis extract and found that it has a strong antibacterial effect under acidic conditions.

[0033] Forsythia is the fruit of the Forsythia suspensa plant, belonging to the Oleaceae family and the Forsythia genus. The fruit is used medicinally; it is slightly cold in nature, bitter in taste, and enters the lung, heart, and gallbladder meridians. It has the effects of clearing heat and detoxifying, reducing swelling and dissipating nodules, and dispersing wind-heat. Forsythia leaves are the mature leaves of Forsythia suspensa, rich in amino acids, vitamins, minerals, and active ingredients such as forsythosides, forsythoside esters, rutin, and quercetin, possessing extremely high medicinal and health-promoting value. Modern pharmacology shows that Forsythia leaves have a similar chemical composition to Forsythia suspensa, with forsythosides and forsythoside esters content far exceeding that of Forsythia suspensa fruit. However, the resources of Forsythia leaves have not been effectively utilized, resulting in significant waste. In recent years, research on the anti-inflammatory, antibacterial, and antioxidant properties of Forsythia leaves has made significant progress. Studies by Wang Tingting et al. have found that the decoction of Forsythia leaves has significant inhibitory activity against nine types of bacteria, including Staphylococcus aureus and Escherichia coli, and also has a good therapeutic effect on chronic inflammation. Studies by Ma Lei et al. have found that the extract of Forsythia suspensa leaves has a better inhibitory effect on Staphylococcus epidermidis than the extract of Forsythia suspensa.

[0034] The main effects of Panax notoginseng are to disperse blood stasis and stop bleeding, reduce swelling and relieve pain; it can be taken internally or applied externally, and it helps blood circulation and promotes the rapid absorption and dispersion of active ingredients.

[0035] Schisandra chinensis, first recorded in the *Shennong Bencao Jing* (Shennong's Classic of Materia Medica), possesses astringent, qi-tonifying, fluid-generating, kidney-tonifying, and heart-calming effects. The 2020 edition of the *Chinese Pharmacopoeia* specifies that the medicinal part of Schisandra chinensis is its dried, mature fruit, which contains lignans, polysaccharides, terpenes, and flavonoids, among other compounds. It exhibits effects such as regulating the central nervous and endocrine systems, anti-tumor activity, antioxidant activity, anti-inflammatory activity, and antibacterial activity.

[0036] Cassia seed: Slightly cold in nature, sweet, salty, and bitter in taste. It clears heat and improves eyesight, moistens the intestines and relieves constipation. Used for red, swollen, and painful eyes, and constipation.

[0037] Dendrobium nobile Lindl., also known as Wan Zhang Xu or Jin Chai Hua, contains the following main active ingredients: dendrobine, dendrobine polysaccharide, dendrobine amine, and dendrobine alkaloid. Studies have shown that Dendrobium nobile has antibacterial, antioxidant, antitumor, immune-enhancing, and nerve-protective effects.

[0038] The above-mentioned medicinal ingredients are extracted with alcohol to form a traditional Chinese medicine compound extract, which has antibacterial, anti-inflammatory, and free radical scavenging effects.

[0039] Based on the extract of this traditional Chinese medicine compound, rose essential oil, isopropyl myristate, cornus seed oil, vitamin E, and other components were further added to prepare a nano-emulsion eye cream, in which:

[0040] Cornus officinalis seed oil is a vegetable edible oil made from Cornus officinalis seeds using traditional pressing techniques. It is rich in various unsaturated fatty acids, with oleic acid accounting for approximately 35.8% and linoleic acid approximately 34.6%. Unsaturated fatty acids play a vital role in human health. Linoleic acid, in particular, is an essential fatty acid that the human body cannot synthesize. Its main function is to soften blood vessels and it plays an important role in metabolism. Linoleic acid can lower blood lipids and blood pressure, thus preventing atherosclerosis and cardiovascular diseases. Furthermore, Cornus officinalis seed oil also contains polyphenols, which have strong antioxidant activity and a good scavenging effect on free radicals.

[0041] Squalane is a hydrocarbon oil obtained by hydrogenating squalene extracted from the liver of deep-sea sharks, hence its other name, deep-sea shark liver oil. Scientific research has found that squalane is one of the few animal oils with high chemical stability and an excellent user experience. It has good affinity with the skin, low polarity, and moderate spreadability.

[0042] Aloe vera gel, sodium hyaluronate, and glycerin are used as moisturizers. Aloe vera gel is a liquid juice obtained from the fleshy part of the leaves of the perennial aloe vera plant (Aloe vera, a member of the lily family). It has moisturizing, sun protection, deodorizing, anti-obesity, skin softening, anti-inflammatory, anti-itch, analgesic, anti-acne, freckle-reducing, and pigmentation-fading effects. Sodium hyaluronate is a glucanuronic acid without species specificity. It is widely found in tissues and organs such as the placenta, amniotic fluid, lens, articular cartilage, and dermis. It is distributed in the cytoplasm and intercellular matrix, playing a lubricating and nourishing role for the cells and organelles contained within them.

[0043] Niacinamide is a water-soluble vitamin and a member of the B vitamins. It can be used as an adjunct treatment for pellagra, relieving skin inflammation, promoting skin recovery, and improving symptoms such as dry and rough skin.

[0044] Rose essential oil has anti-fatigue, antibacterial, and anti-inflammatory effects, and can also add a light floral fragrance.

[0045] Menthol is the main component of peppermint. It is pungent and cool in nature, and enters the lung and liver meridians. It has the functions of dispelling wind and heat, relieving depression, and promoting rash eruption. In traditional Chinese ophthalmology, it is often used in combination with honeysuckle, mulberry leaves, and burdock seeds to treat colds with wind-heat attacking the eyes, causing headache, red eyes, and excessive tearing—specifically, conjunctivitis and corneal inflammation following a cold. The main component of menthol is menthol (C6H2O). 10 H 20 O), main functions: promotes penetration, facilitates the absorption of active ingredients through the skin, and has a cooling effect.

[0046] Lecithin and isopropyl myristate are used as emulsifiers.

[0047] Polysorbate-80 is a surfactant.

[0048] Carrageenan is a thickener.

[0049] Vitamin E is an antioxidant.

[0050] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0051] 1. The herbal compound extract of the present invention uses Chinese medicinal herbs such as Cornus officinalis, Forsythia suspensa, Panax notoginseng, Schisandra chinensis, Dendrobium nobile, and Cassia tora as raw materials. It has antibacterial, anti-inflammatory, and free radical scavenging effects. It is combined through experimental demonstration and combined with the theory of traditional Chinese medicine compatibility. It works synergistically to promote blood circulation in the eye area, clear heat and improve eyesight, and relieve symptoms of bacterial conjunctivitis. Among them, Cornus officinalis and Forsythia suspensa contain rich antioxidants, which can effectively inhibit tyrosinase activity and prevent excessive melanin production from the source, thereby lightening dark circles and age spots.

[0052] 2. This invention selects Forsythia suspensa leaves and menthol, which have a cooling and detoxifying effect; Cornus officinalis, which is slightly warm and tonifies the liver and kidneys; Cassia tora, which is slightly cold and clears the liver and improves eyesight; and Panax notoginseng, which is warm and promotes blood circulation and removes blood stasis. Traditional Chinese medicine believes that the treatment principle for bacterial conjunctivitis is to purge heat and reduce fire, and clear heat and detoxify. The above-mentioned Chinese medicines are used in combination and have a synergistic effect, thereby achieving the purpose of preventing and treating bacterial conjunctivitis.

[0053] 3. This invention uses nanotechnology to evenly disperse the active ingredients, which can promote the drug components to penetrate the stratum corneum of the skin and be delivered deep into the bottom layer of the eye skin. It not only has a good preventive and therapeutic effect on acute bacterial conjunctivitis, but also has the effects of wrinkle removal, whitening, moisturizing and improving eyesight.

[0054] 4. All the herbs used in this invention to prepare compound extracts of traditional Chinese medicine have been included in the "Dual-Use List of Medicines and Foods". The product has stable performance, is mild and non-irritating, has good safety, significant efficacy, few side effects, and does not harm the skin. Attached Figure Description

[0055] Figure 1 The graph shows the anti-inflammatory activity test results of the traditional Chinese medicine compound extracts prepared in Examples 1-5;

[0056] Figure 2 The particle size analysis results are shown in Figure 8 for the nano-emulsion eye cream prepared in Example 8.

[0057] Figure 3 The image shows the zeta site analysis results of the nano-emulsion eye cream prepared in Example 8.

[0058] Figure 4 The image shows the nano-emulsion eye cream product prepared in Example 8. Detailed Implementation

[0059] To make the objectives, technical solutions, and advantages of this invention clearer and more explicit, the invention is further described in detail below through embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0060] (I) Extracts from Traditional Chinese Medicine Compound Formulas

[0061] 1. Screening of components from traditional Chinese medicine compound extracts

[0062] 1.1 Screening by in vitro antibacterial test

[0063] Fourteen medicinal and edible substances were screened by reviewing references. Staphylococcus aureus and Escherichia coli were used as indicator bacteria to determine the minimum inhibitory concentration (MIC) of each Chinese herbal extract. Based on crude drug, MIC < 7.8 mg / mL (highly sensitive), 7.8 mg / mL ≤ MIC ≤ 250 mg / mL (moderately sensitive), and 250 mg / mL ≤ MIC (insensitive). The results are shown in Table 1.

[0064] Table 1 Results of antibacterial effects of different traditional Chinese medicines

[0065]

[0066] Table 1 shows that the top 5 extracts with the best antibacterial effects are Cornus officinalis extract, Forsythia suspensa leaf extract, Panax notoginseng extract, Schisandra chinensis extract, and Dendrobium nobile extract.

[0067] To explore the effects (synergistic or antagonistic, etc.) between combinations of traditional Chinese medicines, five selected herbal extracts were subjected to an in vitro combined antibacterial test. The synergistic index FICI = combined effect / drug A effect + combined effect / drug B effect. FICI ≤ 0.5 indicates synergistic effect, 0.5 ≤ FICI ≤ 1 indicates additive effect, 1 ≤ FICI ≤ 2 indicates no effect, and 2 ≤ FICI indicates antagonistic effect. The results are shown in Table 2.

[0068] Table 2 In vitro combined antibacterial test

[0069]

[0070] As shown in Table 2, the FICI index of Staphylococcus aureus, except for Forsythia suspensa extract and Dendrobium nobile extract which were greater than 1, showed additive or synergistic effects in the other groups. Similarly, the FICI index of Escherichia coli, except for Cornus officinalis extract and Dendrobium nobile extract which were greater than 1, showed additive or synergistic effects in the other groups. This indicates that the antibacterial effect of this combination will be significantly enhanced compared to the single medicinal effect.

[0071] 1.2 Screening by in vitro anti-inflammatory test

[0072] The anti-inflammatory effects of traditional Chinese medicine extracts were evaluated using a RAW 264.7 inflammatory cell model. The specific steps were as follows: RAW264.7 cells were grown in DMEM medium containing 10% fetal bovine serum. Every 24 hours, the cells were passaged in a 5% carbon dioxide (CO2) incubator at 37°C. Stable cells were selected for the experiment, with a seeding density of 1×10⁶ cells / year. 4 The cells were seeded at a rate of 100 μL / mL in 96-well plates. The control group consisted of logarithmic-phase RAW264.7 cells cultured in complete medium, and the model group consisted of LPS-induced cells (500 ng / mL LPS). The results are shown in Table 3.

[0073] Table 3. Effects of different Chinese herbal extracts on inflammatory factors in the supernatant of LPS-induced RAW264.7 inflammatory cells.

[0074] Blank group —— 138.64±31.12 33.68±3.47 21.76±3.82 Model group —— 2327.47±33.47 155.26±2.64 1001.78±56.22 Cornus officinalis extract 60 412.37±26.14 43.65±3.01 101.28±23.13 Forsythia leaf extract 60 207.98±25.31 67.43±2.82 166.42±22.47 Panax notoginseng extract 60 227.62±31.07 87.62±2.54 102.47±10.27 Prunella vulgaris extract 60 721.46±25.66 117.47±2.56 165.23±14.11 Honeysuckle extract 60 347.46±37.12 49.55±3.09 112.48±8.94 Menthol extract 60 777.41±28.21 122.45±2.77 312.23±15.37 Schisandra chinensis extract 60 611.26±16.77 102.31±3.09 147.44±13.26 Polygonatum extract 60 811.42±26.15 131.32±2.63 848.55±31.21 Dendrobium nobile extract 60 317.44±27.77 91.26±4.87 147.38±12.22

[0075] Based on the selection from Table 3, the top 5 extracts are Cornus officinalis extract, Forsythia suspensa leaf extract, Panax notoginseng extract, Lonicera japonica extract, and Dendrobium nobile extract.

[0076] Considering the antibacterial and anti-inflammatory effects, this invention ultimately selected Cornus officinalis, Forsythia suspensa leaves, Panax notoginseng, Schisandra chinensis, and Dendrobium nobile, while adding Cassia tora seeds, which have the effect of improving eyesight, and used their extracts as the main ingredients for preparing the eye cream.

[0077] 2. Preparation of samples of traditional Chinese medicine compound extracts

[0078] Example 1

[0079] A compound extract of traditional Chinese medicine, by weight, is made from the following raw materials: 30 parts Cornus officinalis, 20 parts Forsythia suspensa leaves, 10 parts Panax notoginseng, 10 parts Schisandra chinensis, 25 parts Cassia tora seeds, and 5 parts Dendrobium nobile.

[0080] Its preparation method specifically includes the following steps:

[0081] S1. Mix Cornus officinalis, Forsythia suspensa leaves, Panax notoginseng, Schisandra chinensis, Cassia tora seeds, and Dendrobium nobile, bake at 60℃ for 12 hours, pulverize, and pass through a 40-mesh sieve to obtain Chinese herbal powder;

[0082] S2. Add the Chinese herbal medicine powder to a 70% ethanol solution at a ratio of 1g:30mL. Perform ultrasonic extraction at 60℃ for 2.5h. Then, decolorize with activated carbon at a pH of 6. The amount of activated carbon used is 1.5% of the mass of the Chinese herbal medicine powder. After decolorization, concentrate until the ethanol evaporates. Repeat the extraction 3 times. Combine the concentrates and dry at 60℃ to obtain the compound extract of Chinese herbal medicine.

[0083] Example 2

[0084] A compound extract of traditional Chinese medicine, by weight, is made from the following raw materials: 30 parts Cornus officinalis, 15 parts Forsythia suspensa leaves, 10 parts Panax notoginseng, 10 parts Schisandra chinensis, 25 parts Cassia tora seeds, and 10 parts Dendrobium nobile.

[0085] Its preparation method specifically includes the following steps:

[0086] S1. Mix Cornus officinalis, Forsythia suspensa leaves, Panax notoginseng, Schisandra chinensis, Cassia tora seeds, and Dendrobium nobile, bake at 65℃ for 14 hours, pulverize, and pass through a 40-mesh sieve to obtain Chinese herbal powder;

[0087] S2. Add the Chinese herbal medicine powder to a 70% ethanol solution at a ratio of 1g:30mL. Perform ultrasonic extraction at 60℃ for 2.5h. Then, decolorize with activated carbon at a pH of 6. The amount of activated carbon used is 1.5% of the mass of the Chinese herbal medicine powder. After decolorization, concentrate until the ethanol evaporates. Repeat the extraction 3 times. Combine the concentrates and dry at 65℃ to obtain the compound extract of Chinese herbal medicine.

[0088] Example 3

[0089] A compound extract of traditional Chinese medicine, by weight, is made from the following raw materials: 30 parts Cornus officinalis, 10 parts Forsythia suspensa leaves, 15 parts Panax notoginseng, 10 parts Schisandra chinensis, 25 parts Cassia tora seeds, and 10 parts Dendrobium nobile.

[0090] Its preparation method specifically includes the following steps:

[0091] S1. Mix Cornus officinalis, Forsythia suspensa leaves, Panax notoginseng, Schisandra chinensis, Cassia tora seeds, and Dendrobium nobile, bake at 60℃ for 14 hours, pulverize, and pass through a 40-mesh sieve to obtain Chinese herbal powder;

[0092] S2. Add the Chinese herbal medicine powder to a 70% ethanol solution at a ratio of 1g:50mL. Perform ultrasonic extraction at 60℃ for 3h. Then, decolorize with activated carbon at a pH of 6 and an activated carbon amount of 2% of the mass of the Chinese herbal medicine powder. After decolorization, concentrate until the ethanol evaporates. Repeat the extraction 3 times, combine the concentrates, and dry at 60℃ to obtain the compound extract of Chinese herbal medicine.

[0093] Example 4

[0094] A compound extract of traditional Chinese medicine, by weight, is made from the following raw materials: 25 parts Cornus officinalis, 10 parts Forsythia suspensa leaves, 15 parts Panax notoginseng, 10 parts Schisandra chinensis, 30 parts Cassia tora, and 10 parts Dendrobium nobile.

[0095] Its preparation method specifically includes the following steps:

[0096] S1. Mix Cornus officinalis, Forsythia suspensa leaves, Panax notoginseng, Schisandra chinensis, Cassia tora seeds, menthol, and Dendrobium nobile, bake at 65℃ for 16 hours, pulverize, and pass through a 40-mesh sieve to obtain Chinese herbal powder.

[0097] S2. Add the Chinese herbal powder to a 70% ethanol solution at a ratio of 1g:50mL. Perform ultrasonic extraction at 60℃ for 3h. Then, decolorize with activated carbon at a pH of 6. The amount of activated carbon used is 2% of the mass of the Chinese herbal powder. After decolorization, concentrate until the ethanol evaporates. Repeat the extraction 3 times. Combine the concentrates and dry at 70℃ to obtain the compound extract of Chinese herbal medicine.

[0098] Example 5

[0099] A compound extract of traditional Chinese medicine, by weight, is made from the following raw materials: 20 parts Cornus officinalis, 10 parts Forsythia suspensa leaves, 15 parts Panax notoginseng, 15 parts Schisandra chinensis, 30 parts Cassia tora seeds, and 10 parts Dendrobium nobile.

[0100] Its preparation method specifically includes the following steps:

[0101] S1. Mix Cornus officinalis, Forsythia suspensa leaves, Panax notoginseng, Schisandra chinensis, Cassia tora seeds, and Dendrobium nobile, bake at 60℃ for 16 hours, pulverize, and pass through a 40-mesh sieve to obtain Chinese herbal powder;

[0102] S2. Add the Chinese herbal powder to a 70% ethanol solution at a ratio of 1g:50mL. Perform ultrasonic extraction at 60℃ for 3h. Then, decolorize with activated carbon at a pH of 6. The amount of activated carbon used is 2% of the mass of the Chinese herbal powder. After decolorization, concentrate until the ethanol evaporates. Repeat the extraction 3 times. Combine the concentrates and dry at 70℃ to obtain the compound extract of Chinese herbal medicine.

[0103] 3. Performance testing of extracts from traditional Chinese medicine compound formulas

[0104] 3.1 Antibacterial activity test

[0105] (1) Preparation of medicinal solution: Weigh the traditional Chinese medicine compound extracts prepared in Examples 1 to 5, prepare a suspension, bottle it, sterilize it, and store it at 4°C.

[0106] (2) Preparation of pathogenic bacterial suspension: Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) O8, frozen at -80℃, were activated, multiplied, and preserved in nutrient broth on slant agar. The activated bacteria were inoculated into culture medium (37℃, 5% CO2, cultured for 24 h), and the bacterial cells were washed with physiological saline to prepare a suspension with a concentration of 1×10⁻⁶. 8 CFU / mL bacterial suspension.

[0107] (3) Determination of the minimum inhibitory concentration (MIC): Take a 96-well plate, and add 200 μL of the drug solution diluted twice with broth to wells 1-8 of each row as test wells. Well 9 is designated as a positive control well (no drug added), and well 10 as a negative control well (no pathogen added). Add 100 μL of 1.0 × 10⁻⁶ ppm of the diluted MIC solution to wells 1-9. 6 CFU / mL pathogenic bacterial solution. After shaking and mixing, incubate at 37℃ for 24 hours and observe the culture results. Using the negative control and positive control wells as references, if wells 1-8 are the same as the positive control (turbid, indicating bacterial growth); if wells 1-8 are the same as the negative control (clear, indicating no bacterial growth); the lowest concentration with no bacterial growth is the MIC of the herb.

[0108] The test results are shown in Table 4.

[0109] Table 4. Antibacterial activity tests of the traditional Chinese medicine compound extracts from Examples 1-5.

[0110]

[0111] As can be seen from Table 4, Example 1 showed a strong antibacterial effect against Staphylococcus aureus and Escherichia coli O8. This is related to the proportion of high-intensity antibacterial Chinese medicinal materials such as Cornus officinalis and Forsythia suspensa. Based on previous research, the MIC values ​​of the antibacterial effect of Cornus officinalis and Forsythia suspensa are much lower than those of the other raw materials, indicating that the antibacterial effect is strong.

[0112] 3.2 Anti-inflammatory activity test

[0113] (1) Cell culture: RAW264.7 cells were grown in DMEM medium containing 10% FBS and cultured in an incubator at 37°C and 5% CO2.

[0114] (2) Drug administration and treatment: After culture, cells were seeded into 96-well plates and induced with 500 ng / mL LPS. Then, the traditional Chinese medicine compound extracts prepared in Examples 1-5 (50 μg / mL) were added. The cells were divided into Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, control group (RAW264.7 cells cultured in complete culture medium), and model group (RAW264.7 cells induced with 500 ng / mL LPS). After culturing for 24 h, further treatment was performed.

[0115] (3) The concentrations of IL-1β, IL-6 and TNF-α were detected by ELISA, and the assay was performed according to the kit instructions.

[0116] Test results are as follows Figure 1 As shown.

[0117] Depend on Figure 1 It can be seen that, compared with the control group, the concentrations of IL-1β, IL-6 and TNF-α in the model group were significantly increased. Compared with the model group, the concentrations of IL-1β, IL-6 and TNF-α in Example 1, Example 2, Example 3, Example 4 and Example 5 groups were significantly decreased, indicating that the extract has obvious anti-inflammatory effects.

[0118] (II) Nano-emulsion eye cream

[0119] 1. Preparation of Nano-Emulsion Eye Cream Samples

[0120] Example 6

[0121] A nano-emulsion eye cream containing a compound extract of traditional Chinese medicine, comprising the following components by mass fraction: 2% of the compound extract of traditional Chinese medicine prepared in Example 1, 0.5% of rose essential oil, 5% of isopropyl myristate, 10% of Cornus officinalis seed oil, 1.8% of vitamin E, 1% of squalane, 0.4% of sodium hyaluronate, 3% of glycerin, 0.5% of niacinamide, 1% of aloe vera gel, 3% of carrageenan, 2% of lecithin, 4% of polysorbate-80, 0.8% of menthol, and 65% of water;

[0122] Its preparation method specifically includes the following steps:

[0123] (1) Dissolve the compound extract of traditional Chinese medicine in acetone at a ratio of 30 mg: 1 mL, then add distilled water with pH 8 and heat until the acetone evaporates to obtain a suspension of traditional Chinese medicine; mix isopropyl myristate, cornus officinalis seed oil, rose essential oil, vitamin E, squalane, lecithin, glycerin and polysorbate-80 thoroughly to obtain an oil phase solution;

[0124] (2) After mixing the traditional Chinese medicine suspension obtained in step (1) with the oil phase solution, homogenize it 20 times under a pressure of 300 bar to obtain a nanoemulsion.

[0125] (3) Sodium hyaluronate, nicotinamide, aloe vera gel, carrageenan and menthol are fully dissolved in water at 40°C to obtain a mixed solution. The nanoemulsion obtained in step (2) is added to the mixed solution and mixed well. Vacuum sterilization is performed to obtain a nanoemulsion eye cream containing Chinese herbal compound extract.

[0126] Example 7

[0127] A nano-emulsion eye cream containing a compound extract of traditional Chinese medicine, comprising the following components by mass fraction: 3% of the compound extract of traditional Chinese medicine prepared in Example 2, 0.75% of rose essential oil, 7% of isopropyl myristate, 10% of Cornus officinalis seed oil, 2% of vitamin E, 1.5% of squalane, 0.5% of sodium hyaluronate, 4% of glycerin, 0.5% of niacinamide, 2% of aloe vera gel, 2% of carrageenan, 6% of lecithin, 3% of polysorbate-80, 0.75% of menthol, and 57% of water;

[0128] Its preparation method specifically includes the following steps:

[0129] (1) Dissolve the compound extract of traditional Chinese medicine in acetone at a ratio of 15 mg: 1 mL, then add distilled water with a pH of 10 and heat until the acetone evaporates to obtain a suspension of traditional Chinese medicine; mix isopropyl myristate, cornus officinalis seed oil, rose essential oil, vitamin E, squalane, lecithin, glycerin and polysorbate-80 thoroughly to obtain an oil phase solution;

[0130] (2) After mixing the traditional Chinese medicine suspension obtained in step (1) with the oil phase solution, homogenize it 20 times under a pressure of 300 bar to obtain a nanoemulsion.

[0131] (3) Sodium hyaluronate, nicotinamide, aloe vera gel, carrageenan and menthol are fully dissolved in water at 40°C to obtain a mixed solution. The nanoemulsion obtained in step (2) is added to the mixed solution and mixed well. Vacuum sterilization is performed to obtain a nanoemulsion eye cream containing Chinese herbal compound extract.

[0132] Example 8

[0133] A nano-emulsion eye cream containing a compound extract of traditional Chinese medicine, comprising the following components by mass fraction: 5% of the compound extract of traditional Chinese medicine prepared in Example 3, 1.25% of rose essential oil, 7% of isopropyl myristate, 8% of Cornus officinalis seed oil, 1.5% of vitamin E, 2% of squalane, 0.5% of sodium hyaluronate, 5% of glycerin, 0.8% of niacinamide, 3% of aloe vera gel, 1% of carrageenan, 8% of lecithin, 4% of polysorbate-80, 0.6% of menthol, and 52.35% of water;

[0134] Its preparation method specifically includes the following steps:

[0135] (1) Dissolve the compound extract of traditional Chinese medicine in acetone at a ratio of 30 mg: 1 mL, then add distilled water with a pH of 13 and heat until the acetone evaporates to obtain a suspension of traditional Chinese medicine; mix isopropyl myristate, Cornus officinalis seed oil, rose essential oil, vitamin E, squalane, lecithin, glycerin and polysorbate-80 thoroughly to obtain an oil phase solution;

[0136] (2) After mixing the traditional Chinese medicine suspension obtained in step (1) with the oil phase solution, homogenize it 20 times under a pressure of 300 bar to obtain a nanoemulsion.

[0137] (3) Sodium hyaluronate, nicotinamide, aloe vera gel, carrageenan and menthol are fully dissolved in water at 40°C to obtain a mixed solution. The nanoemulsion obtained in step (2) is added to the mixed solution and mixed well. Vacuum sterilization is performed to obtain a nanoemulsion eye cream containing Chinese herbal compound extract.

[0138] Example 9

[0139] A nano-emulsion eye cream containing a compound extract of traditional Chinese medicine, comprising the following components by mass fraction: 7% of the compound extract of traditional Chinese medicine prepared in Example 4, 1.5% of rose essential oil, 9% of isopropyl myristate, 6% of Cornus officinalis seed oil, 1.5% of vitamin E, 2% of squalane, 1.5% of sodium hyaluronate, 6% of glycerin, 0.8% of niacinamide, 1% of aloe vera gel, 1% of carrageenan, 6% of lecithin, 3% of polysorbate-80, 0.5% of menthol, and 53.2% of water;

[0140] Its preparation method specifically includes the following steps:

[0141] (1) Dissolve the compound extract of traditional Chinese medicine in acetone at a ratio of 30 mg: 1 mL, then add distilled water with a pH of 10 and heat until the acetone evaporates to obtain a suspension of traditional Chinese medicine; mix isopropyl myristate, cornus officinalis seed oil, rose essential oil, vitamin E, squalane, lecithin, glycerin and polysorbate-80 thoroughly to obtain an oil phase solution;

[0142] (2) After mixing the traditional Chinese medicine suspension obtained in step (1) with the oil phase solution, homogenize it 20 times under a pressure of 300 bar to obtain a nanoemulsion.

[0143] (3) Sodium hyaluronate, nicotinamide, aloe vera gel, carrageenan and menthol are fully dissolved in water at 40°C to obtain a mixed solution. The nanoemulsion obtained in step (2) is added to the mixed solution and mixed well. Vacuum sterilization is performed to obtain a nanoemulsion eye cream containing Chinese herbal compound extract.

[0144] Example 10

[0145] A nano-emulsion eye cream containing a compound extract of traditional Chinese medicine, comprising the following components by mass fraction: 8% of the compound extract of traditional Chinese medicine prepared in Example 5, 2% of rose essential oil, 4% of isopropyl myristate, 11% of Cornus officinalis seed oil, 1.5% of vitamin E, 3% of squalane, 2% of sodium hyaluronate, 7% of glycerin, 0.8% of niacinamide, 3% of aloe vera gel, 1% of carrageenan, 8% of lecithin, 3% of polysorbate-80, 0.4% of menthol, and 43.3% of water;

[0146] Its preparation method specifically includes the following steps:

[0147] (1) Dissolve the compound extract of traditional Chinese medicine in acetone at a ratio of 30 mg: 1 mL, then add distilled water with pH 8 and heat until the acetone evaporates to obtain a suspension of traditional Chinese medicine; mix isopropyl myristate, cornus officinalis seed oil, rose essential oil, vitamin E, squalane, lecithin, glycerin and polysorbate-80 thoroughly to obtain an oil phase solution;

[0148] (2) After mixing the traditional Chinese medicine suspension obtained in step (1) with the oil phase solution, homogenize it 20 times under a pressure of 300 bar to obtain a nanoemulsion.

[0149] (3) Sodium hyaluronate, nicotinamide, aloe vera gel, carrageenan and menthol are fully dissolved in water at 40°C to obtain a mixed solution. The nanoemulsion obtained in step (2) is added to the mixed solution and mixed well. Vacuum sterilization is performed to obtain a nanoemulsion eye cream containing Chinese herbal compound extract.

[0150] 2. Performance Testing of Nano-Emulsion Eye Cream

[0151] 2.1 Particle Size Analysis

[0152] The nano-emulsion eye cream prepared in Example 8 was diluted 10-fold, and its average particle size (Z-average) and polydispersity index (PDI) were determined using a Zetasizer Nano ZS analyzer. The results are as follows. Figure 2 As shown.

[0153] Depend on Figure 1 It can be seen that at a normal temperature of 25℃, the particle size of the nanoemulsion eye cream is within the range of ≤100nm, which is consistent with the characteristics of nanoemulsion. Its average particle size is 67nm and PDI is 0.387.

[0154] 2.2 Zeta Point Analysis

[0155] The nano-emulsion eye cream prepared in Example 8 was diluted 10 times, and its Zeta potential was measured using a Malvern potential analyzer. The measurement results are as follows: Figure 3 As shown.

[0156] Depend on Figure 3 It can be seen that its average potential is +4.24, at which the nanoemulsion has good stability.

[0157] 2.3 Product Samples

[0158] Example 8 shows the preparation of a nano-emulsion eye cream sample. Figure 4 As shown, the sample has a fine and smooth texture. Therefore, it can be applied smoothly, absorbed quickly, and spreads well during use.

[0159] 2.4 Efficacy Evaluation

[0160] Survey period: December 1, 2023 to June 1, 2024, a period of 6 months; Survey subjects: those who work long hours, stay up late, and have eye diseases such as keratitis and conjunctivitis; Number of survey participants: 120.

[0161] The survey participants were randomly divided into 5 groups, each using the nano-emulsion eye cream prepared in Examples 6-10: Experimental Group 1 (Example 6), Experimental Group 2 (Example 7), Experimental Group 3 (Example 8), Experimental Group 4 (Example 9), Experimental Group 5 (Example 10), and a control group (commercially available ordinary eye cream). Each group consisted of 20 participants. The survey was conducted according to the usage instructions. Evaluation criteria included comfort, moisturizing effect, reduction of dark circles, and relief of conjunctivitis. Evaluation methods included significantly effective (good), moderately effective (fair), and ineffective (poor or ineffective). The total effective rate was calculated as (significantly effective + moderately effective) / total number of experiments.

[0162] How to use: Take an amount of nano-emulsion eye cream the size of a mung bean and apply it evenly to the under-eye bags, corners of the eyes, or the area around the eyes (avoid getting into the eyes). Then gently massage for 1 to 5 minutes (3 to 5 minutes for best results) 3 to 5 times a day.

[0163] The survey results are shown in Table 5.

[0164] Table 5. Results of the trial population survey

[0165]

[0166]

[0167] As shown in Table 5, after 6 months of trial use, the total effective rates of Examples 6 to 10 were 88.75%, 98.75%, 97.5%, 98.75%, and 92.5%, respectively, all of which were better than the control group of 77.5%. This indicates that the product has a moisturizing effect on the skin, can improve dark circles, and alleviate the symptoms of conjunctivitis.

[0168] The foregoing has described preferred embodiments of the present invention. However, it should be understood that the invention is not limited to the content disclosed herein. Any non-substantial improvements made using the inventive concept and technical solution, or any application of the inventive concept and technical solution to other situations, are within the protection scope of the present invention.

Claims

1. A traditional Chinese medicine compound extract with anti-inflammatory and antibacterial effects, characterized in that, It is made from the following raw materials in parts by weight: 20-35 parts of Cornus officinalis, 10-20 parts of Forsythia suspensa leaves, 10-20 parts of Panax notoginseng, 10-15 parts of Schisandra chinensis, 25-30 parts of Cassia tora seeds, and 5-15 parts of Dendrobium nobile. The preparation method of the traditional Chinese medicine compound extract includes the following steps: S1. Mix Cornus officinalis, Forsythia suspensa leaves, Panax notoginseng, Schisandra chinensis, Cassia tora seeds, and Dendrobium nobile, bake, pulverize, and sieve to obtain Chinese medicine powder; S2. The Chinese herbal powder is added to an ethanol solution for ultrasonic extraction, then decolorized and concentrated until the ethanol evaporates. The extraction is repeated 3 times. The concentrated liquids are combined and dried to obtain the Chinese herbal compound extract. The volume concentration of the ethanol solution is 60% to 80%, and the weight-volume ratio of the Chinese herbal powder to the ethanol solution is 1g:(30 to 60)mL.

2. The traditional Chinese medicine compound extract as described in claim 1, characterized in that, The ultrasonic extraction temperature in step S2 is 60-80℃, and the time is 2-4 hours.

3. The traditional Chinese medicine compound extract as described in claim 1, characterized in that, The decolorization in step S2 uses activated carbon, with a decolorization pH of 6-8, and the amount of activated carbon used is 0.5%-3% of the mass of the Chinese medicine powder.

4. The application of the traditional Chinese medicine compound extract as described in claim 1 in the preparation of eye cream.

5. A nano-emulsion eye cream containing extracts of a traditional Chinese medicine compound, characterized in that, It consists of the following components by weight percentage: The traditional Chinese medicine compound extract of claim 1 comprises 2-8% rose essential oil, 0.5-2% isopropyl myristate, 4-12% Cornus officinalis seed oil, 1.5-3% vitamin E, 1-3% squalane, 0.05-2% sodium hyaluronate, 3-7% glycerin, 0.4-0.8% nicotinamide, 1-3% aloe vera gel, 1-3% carrageenan, 2-8% lecithin, 1-5% polysorbate-80, 0.3-0.8% menthol, with the balance being water.

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