Method for enhancing antibiotic / estrogen reduction in a facility soil high temperature shed
Patent Information
- Application Number
- CN202411278124.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-12
- Publication Date
- 2026-09-15
- Estimated Expiration
- 2044-09-12
AI Technical Summary
抗生素耐药菌体内携带的抗生素抗性基因是一种生物污染物,它在可移动遗传元件的作用下可在不同种微生物之间进行水平基因转移,从而增加了随食物链向人体转移的风险
[0023] This invention provides a method for enhancing antibiotic/estrogen reduction in greenhouse soil through high-temperature fumigation. The method involves thoroughly watering the greenhouse soil and covering it with a film. Utilizing the higher thermal conductivity of water compared to soil, the topsoil layer reaches a high temperature, effectively disinfecting and sterilizing it. The soil is disinfected and sterilized through the combined effects of solar energy storage, biomass energy production, and chemical energy release. This invention, on the one hand, alters the soil's carbon-nitrogen ratio by adding tomato straw, providing ample carbon and nitrogen sources for soil microorganisms and promoting their degradation of antibiotics/estrogens; on the other hand, it regulates soil moisture content and the effective fumigation time to further increase soil temperature and promote antibiotic/estrogen degradation under high temperatures.
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Figure CN118891989B_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of agricultural production, and in particular relates to a method for enhancing the reduction of antibiotics / estrogens in facility soil by high-temperature fumigation. Background Technology
[0002] Antibiotics are secondary metabolites produced by microorganisms (including bacteria and fungi) or higher plants and animals during metabolism, possessing antipathogenic or other active substances. Since their discovery in 1928, antibiotics have been widely used in the medical and health industry for disease treatment and prevention. Subsequently, antibiotics were widely used as feed additives in livestock and poultry farming. With rapid economic development and increasing demand for meat, the livestock industry, in its pursuit of faster economic output and animal growth, has seen a surge in antibiotic abuse and misuse. However, once antibiotics enter the body, they are not completely absorbed; approximately 30%-90% are excreted in the form of the original drug or metabolites through livestock and poultry feces and urine. This abuse and misuse in livestock farming directly exacerbates the accumulation and residue of antibiotics in the environment. With the development of intensive livestock farming, livestock and poultry manure, after untreated or minimally treated processes, is mostly applied to farmland as organic fertilizer. Studies have shown that the processing of organic fertilizer cannot completely eliminate antibiotics, resulting in antibiotics in livestock and poultry manure entering the soil and aquatic environments through organic fertilizer application. The accumulation and residue of antibiotics in the environment can lead to antibiotic resistance problems. Antibiotics have become one of the four new pollutants controlled by international conventions, characterized by strong biotoxicity, environmental persistence, and bioaccumulation. Facility soils, due to the high frequency and amount of organic fertilizer use, have become typical areas of antibiotic pollution. Antibiotics entering the soil environment cause many environmental problems. High concentrations of antibiotic residues in the soil force soil microorganisms to continuously evolve in order to survive, stimulating corresponding resistance mechanisms, leading to the emergence of antibiotic-resistant bacteria and even superbugs. The antibiotic resistance genes carried by antibiotic-resistant bacteria are biological pollutants; under the action of mobile genetic elements, they can undergo horizontal gene transfer between different microbial species, thereby increasing the risk of transfer to humans through the food chain. Currently, antibiotic reduction measures are mostly focused on the livestock sector, lacking universally applicable methods for reduction in farmland. Therefore, reducing antibiotic residues in facility soils is of great significance.
[0003] Endocrine disruptors are also among the emerging pollutants, with estrogen belonging to a class of endocrine disruptors that exhibit strong environmental toxicity. Estrogen in the environment mainly originates from animal secretions during livestock and poultry farming and the introduction of exogenous additives. Unable to be fully utilized by animals, it enters the environment through livestock and poultry feces, disrupting the endocrine systems of aquatic organisms and damaging the aquatic environment.
[0004] Therefore, how to reduce antibiotics / estrogens in the soil of high-temperature fumigation facilities has become an urgent problem to be solved. Summary of the Invention
[0005] The main objective of this invention is to provide a method for enhancing the reduction of antibiotics / estrogens in facility soil through high-temperature fumigation, thereby overcoming the shortcomings of the prior art.
[0006] To achieve the aforementioned objectives, the technical solution adopted by this invention includes:
[0007] According to a first aspect of the present invention, a method for enhancing the reduction of antibiotics / estrogens in facility soil by high-temperature fumigation is provided, comprising the following steps:
[0008] S1. During the high-temperature fallow period in the greenhouse, after the previous crop is harvested, remove vegetable residues from the greenhouse, inspect the greenhouse equipment, and ensure the normal use and sealing of the greenhouse film for future use.
[0009] S2. After crushing the tomato stalks, spread them, then use a rotary tiller to turn them over and till them to obtain furrows;
[0010] S3. Bury the ground temperature monitoring probe in the cultivated ditch, then lay the drip irrigation pipe, and then carry out drip irrigation after sealing the greenhouse film;
[0011] S4. Cover the soil inside the greenhouse with black plastic film and irrigate it until the water level inside the greenhouse reaches the preset conditions.
[0012] S5. After closing the ventilation openings of the greenhouse, conduct high-temperature fumigation.
[0013] Furthermore, in step S2, 550-650 kg of the tomato straw is spread per acre.
[0014] Furthermore, in step S2, the tomato straw is crushed, mixed with organic fertilizer, and then spread, while maintaining a carbon-nitrogen ratio of 16-19:1.
[0015] Furthermore, apply 450-550 kg of organic fertilizer per acre.
[0016] Furthermore, in step S2, the rotary tiller's turning depth is 15-25cm.
[0017] Furthermore, in step S3, the ground temperature monitoring probe is buried at a depth of 7.5-12.5cm in the cultivated ditch.
[0018] Furthermore, in step S3, drip irrigation is carried out for 7-10 days after the greenhouse film is sealed.
[0019] Furthermore, the preset conditions mentioned in step S4 refer to controlling the irrigation amount to 100% of the field capacity and to be 2-4 cm above the ground.
[0020] Furthermore, in step S5, the high-temperature fumigation period is 25-35 days.
[0021] Furthermore, in step S5, the temperature is maintained above 55°C for at least 4-6 days during the high-temperature fumigation period.
[0022] Compared with the prior art, the advantages of the present invention include:
[0023] This invention provides a method for enhancing antibiotic / estrogen reduction in greenhouse soil through high-temperature fumigation. The method involves thoroughly watering the greenhouse soil and covering it with a film. Utilizing the higher thermal conductivity of water compared to soil, the topsoil layer reaches a high temperature, effectively disinfecting and sterilizing it. The soil is disinfected and sterilized through the combined effects of solar energy storage, biomass energy production, and chemical energy release. This invention, on the one hand, alters the soil's carbon-nitrogen ratio by adding tomato straw, providing ample carbon and nitrogen sources for soil microorganisms and promoting their degradation of antibiotics / estrogens; on the other hand, it regulates soil moisture content and the effective fumigation time to further increase soil temperature and promote antibiotic / estrogen degradation under high temperatures. Attached Figure Description
[0024] Various other advantages and benefits will become apparent to those skilled in the art upon reading the following detailed description of preferred embodiments. The accompanying drawings are for illustrative purposes only and are not intended to limit the invention. In the drawings:
[0025] Figure 1 This is a diagram showing the tylosin residue after treatment in the experimental examples of this invention.
[0026] Figure 2 This is a diagram showing the tetracycline residue after treatment in the experimental examples of this invention;
[0027] Figure 3 This is a diagram showing the residue of sulfadiazine after treatment in the experimental examples of this invention;
[0028] Figure 4 This is a diagram showing the enrofloxacin residue after treatment in the experimental examples of this invention.
[0029] Figure 5 This is a diagram showing the estrone residue after treatment in the experimental examples of this invention;
[0030] Figure 6 This is a diagram showing the residual estriol after treatment in the experimental examples of this invention. Detailed Implementation
[0031] In view of the shortcomings of the prior art, the inventors of this invention, through long-term research and extensive practice, have proposed the technical solution of this invention. The following will further explain and illustrate this technical solution, its implementation process, and its principles.
[0032] The present invention will now be described in further detail with reference to specific embodiments. The given embodiments are merely illustrative of the invention and not intended to limit its scope. The embodiments provided below can serve as a guide for further improvements by those skilled in the art and do not constitute a limitation on the invention in any way.
[0033] Unless otherwise specified, the experimental methods used in the following examples are conventional methods, performed according to the techniques or conditions described in the literature in this field or according to the product instructions. Unless otherwise specified, the materials and reagents used in the following examples are commercially available.
[0034] This invention provides a method for enhancing antibiotic / estrogen reduction in facility soil through high-temperature fumigation, comprising the following steps:
[0035] S1. During the high-temperature fallow period in the greenhouse, after the previous crop is harvested, remove the vegetable residues in the greenhouse, inspect the greenhouse equipment, and ensure the normal use and sealing of the greenhouse film for future use; the high-temperature fallow period refers to the period during the summer when, due to the crop growth cycle, the farmland is temporarily not planted, and this period coincides with the high-temperature weather.
[0036] S2. After crushing the tomato straw, spread it, then use a rotary tiller to turn it over and till it to obtain furrows; spread 550-650 kg of the tomato straw per acre; in some embodiments, the tomato straw is crushed and mixed with organic fertilizer before spreading, and the carbon-nitrogen ratio is maintained at 16-19:1; in some more specific embodiments, 450-550 kg of organic fertilizer is spread per acre; the rotary tiller turns the straw to a depth of 15-25 cm;
[0037] S3. Bury a ground temperature monitoring probe at a depth of 7.5-12.5cm in the cultivated ditch, then lay drip irrigation pipes, and seal the greenhouse film for drip irrigation for 7-10 days.
[0038] S4. Cover the soil inside the greenhouse with black plastic film and irrigate until the water level inside the greenhouse reaches the preset conditions; the preset conditions refer to controlling the irrigation water volume to 100% of the field capacity and 2-4 cm above the ground.
[0039] S5. After closing the ventilation vents of the facility greenhouse, conduct high-temperature fumigation for 25-35 days, during which the temperature is maintained above 55℃ for at least 4-6 days.
[0040] The method provided in this invention disinfects and sterilizes soil through the combined effects of three major thermal energy sources: solar energy storage, bioenergy production, and chemical energy release. Solar energy storage refers to using solar energy to directly heat the soil through sunlight, increasing soil temperature and thus killing pathogens and pests. Bioenergy production refers to using the heat generated by the activity of microorganisms in organic fertilizer to increase soil temperature; simultaneously, these beneficial bacteria can inhibit and kill harmful microorganisms in the soil. Chemical energy release refers to using the exothermic reaction of chemical substances to increase soil temperature; simultaneously, the disinfecting effect of the chemical substances themselves kills pathogens and pests. For example, chemical disinfectants such as formalin, carbendazim, and thiophanate-methyl are sprayed or mixed into the soil, utilizing their disinfecting and sterilizing effects to kill pathogens in the soil. Furthermore, this invention, on the one hand, alters the soil carbon-nitrogen ratio by adding tomato straw, providing sufficient carbon and nitrogen sources for soil microorganisms and promoting the microbial degradation of antibiotics / estrogens; on the other hand, it regulates soil moisture content and effective fumigation time to further increase soil temperature and promote the degradation of antibiotics / estrogens under high temperatures.
[0041] Example 1
[0042] This embodiment provides a method for enhancing the reduction of antibiotics / estrogens in facility soil through high-temperature fumigation, including the following steps:
[0043] S1. During the high-temperature fallow period in the greenhouse, after the previous crop is harvested, remove vegetable residues from the greenhouse, inspect the greenhouse equipment, and ensure the normal use and sealing of the greenhouse film for future use.
[0044] S2. Crush the tomato stalks and spread them, then use a rotary tiller to turn them over and till them to obtain furrows; spread 550 kg of the tomato stalks per acre; the rotary tiller turns the stalks to a depth of 15 cm;
[0045] S3. Bury a ground temperature monitoring probe at a depth of 7.5cm in the cultivated ditch, then lay drip irrigation pipes, and seal the greenhouse film for drip irrigation for 7 days;
[0046] S4. Cover the soil inside the greenhouse with black plastic film and irrigate until the water level inside the greenhouse reaches the preset conditions; the preset conditions refer to controlling the irrigation water volume to 100% of the field capacity and 2cm above the ground.
[0047] S5. After closing the ventilation vents of the facility greenhouse, conduct high-temperature fumigation for 25 days, during which the temperature is maintained above 55°C for at least 4 days.
[0048] Example 2
[0049] This embodiment provides a method for enhancing the reduction of antibiotics / estrogens in facility soil through high-temperature fumigation, including the following steps:
[0050] S1. During the high-temperature fallow period in the greenhouse, after the previous crop is harvested, remove vegetable residues from the greenhouse, inspect the greenhouse equipment, and ensure the normal use and sealing of the greenhouse film for future use.
[0051] S2. Crush the tomato stalks and spread them, then use a rotary tiller to turn them over and till them to obtain furrows; spread 600 kg of the tomato stalks per acre; the rotary tiller turns the stalks to a depth of 20 cm;
[0052] S3. Bury a ground temperature monitoring probe at a depth of 10cm in the cultivated ditch, then lay drip irrigation pipes, and seal the greenhouse film for drip irrigation for 8.5 days.
[0053] S4. Cover the soil inside the greenhouse with black plastic film and irrigate until the water level inside the greenhouse reaches the preset conditions; the preset conditions refer to controlling the irrigation water volume to be 100% of the field capacity and 3cm above the ground.
[0054] S5. After closing the ventilation vents of the facility greenhouse, conduct high-temperature fumigation for 30 days, during which the temperature is maintained above 55°C for at least 5 days.
[0055] Example 3
[0056] This embodiment provides a method for enhancing the reduction of antibiotics / estrogens in facility soil through high-temperature fumigation, including the following steps:
[0057] S1. During the high-temperature fallow period in the greenhouse, after the previous crop is harvested, remove vegetable residues from the greenhouse, inspect the greenhouse equipment, and ensure the normal use and sealing of the greenhouse film for future use.
[0058] S2. Crush the tomato stalks and spread them, then use a rotary tiller to turn them over and till them to obtain furrows; spread 650 kg of the tomato stalks per acre; the rotary tiller turns the stalks to a depth of 25 cm;
[0059] S3. Bury a ground temperature monitoring probe at a depth of 12.5cm in the cultivated ditch, then lay drip irrigation pipes, seal the greenhouse film and carry out drip irrigation for 10 days;
[0060] S4. Cover the soil inside the greenhouse with black plastic film and irrigate until the water level inside the greenhouse reaches the preset conditions; the preset conditions refer to controlling the irrigation water volume to 100% of the field capacity and 4cm above the ground.
[0061] S5. After closing the ventilation vents of the facility greenhouse, conduct high-temperature fumigation for 35 days, during which the temperature is maintained above 55°C for at least 6 days.
[0062] Example 4
[0063] This embodiment provides a method for enhancing the reduction of antibiotics / estrogens in facility soil through high-temperature fumigation, including the following steps:
[0064] S1. During the high-temperature fallow period in the greenhouse, after the previous crop is harvested, remove vegetable residues from the greenhouse, inspect the greenhouse equipment, and ensure the normal use and sealing of the greenhouse film for future use.
[0065] S2. After crushing the tomato straw, spread it, then use a rotary tiller to turn it over and till it to obtain furrows; spread 550 kg of the tomato straw per acre; spread 450 kg of organic fertilizer per acre; and maintain a carbon-nitrogen ratio of 16:1; the rotary tiller turns the straw to a depth of 15 cm;
[0066] S3. Bury a ground temperature monitoring probe at a depth of 7.5cm in the cultivated ditch, then lay drip irrigation pipes, and seal the greenhouse film for drip irrigation for 7 days;
[0067] S4. Cover the soil inside the greenhouse with black plastic film and irrigate until the water level inside the greenhouse reaches the preset conditions; the preset conditions refer to controlling the irrigation water volume to 100% of the field capacity and 2cm above the ground.
[0068] S5. After closing the ventilation vents of the facility greenhouse, conduct high-temperature fumigation for 25 days, during which the temperature is maintained above 55°C for at least 4 days.
[0069] Example 5
[0070] This embodiment provides a method for enhancing the reduction of antibiotics / estrogens in facility soil through high-temperature fumigation, including the following steps:
[0071] S1. During the high-temperature fallow period in the greenhouse, after the previous crop is harvested, remove vegetable residues from the greenhouse, inspect the greenhouse equipment, and ensure the normal use and sealing of the greenhouse film for future use.
[0072] S2. Crush the tomato stalks and spread them, then use a rotary tiller to turn them over and till them to obtain furrows; spread 600 kg of the tomato stalks per acre; spread 450-550 kg of organic fertilizer per acre; and maintain a carbon-nitrogen ratio of 17.5:1; the rotary tiller turns the stalks to a depth of 20 cm;
[0073] S3. Bury a ground temperature monitoring probe at a depth of 10cm in the cultivated ditch, then lay drip irrigation pipes, and seal the greenhouse film for drip irrigation for 8.5 days.
[0074] S4. Cover the soil inside the greenhouse with black plastic film and irrigate until the water level inside the greenhouse reaches the preset conditions; the preset conditions refer to controlling the irrigation water volume to be 100% of the field capacity and 3cm above the ground.
[0075] S5. After closing the ventilation vents of the facility greenhouse, conduct high-temperature fumigation for 30 days, during which the temperature is maintained above 55°C for at least 5 days.
[0076] Example 6
[0077] This embodiment provides a method for enhancing the reduction of antibiotics / estrogens in facility soil through high-temperature fumigation, including the following steps:
[0078] S1. During the high-temperature fallow period in the greenhouse, after the previous crop is harvested, remove vegetable residues from the greenhouse, inspect the greenhouse equipment, and ensure the normal use and sealing of the greenhouse film for future use.
[0079] S2. After crushing the tomato straw, spread it, then use a rotary tiller to turn it over and till it to obtain furrows; spread 650 kg of the tomato straw per acre; spread 550 kg of organic fertilizer per acre; and maintain a carbon-nitrogen ratio of 19:1; the rotary tiller turns the straw to a depth of 25 cm;
[0080] S3. Bury a ground temperature monitoring probe at a depth of 12.5cm in the cultivated ditch, then lay drip irrigation pipes, seal the greenhouse film and carry out drip irrigation for 10 days;
[0081] S4. Cover the soil inside the greenhouse with black plastic film and irrigate until the water level inside the greenhouse reaches the preset conditions; the preset conditions refer to controlling the irrigation water volume to 100% of the field capacity and 4cm above the ground.
[0082] S5. After closing the ventilation vents of the facility greenhouse, conduct high-temperature fumigation for 35 days, during which the temperature is maintained above 55°C for at least 6 days.
[0083] The organic fertilizer in Examples 4-6 refers to the conventional organic fertilizer used in the park, which is made by composting cow manure and horse manure in a 1:1 ratio.
[0084] Experimental Example
[0085] Untreated soil was used as the control group, and treatments 1, 2, and 3 were used as experimental groups.
[0086] Treatment 1 showed the following values: tylosin 133.3 mg / kg, tetracycline 23.3 mg / kg, sulfadiazine 15.66 mg / kg, enrofloxacin 217.3 ug / kg, estrone 1507.4 ug / kg, and estriol 40.75 ug / kg. This treatment was administered during the high-temperature fallow period in greenhouses during July and August. After the previous crop was harvested, vegetable residues inside the greenhouse were cleaned, and the greenhouse film was repaired to ensure sealing and insulation. After crushing fresh tomato stalks (approximately 600 kg / acre), spread the stalks and then use a rotary tiller to turn them over to a depth of 20 cm, ensuring even tillage as much as possible. Bury a soil temperature monitoring probe at a depth of 10 cm and lay drip irrigation pipes, then seal the greenhouse film for drip irrigation for about 8.5 days. Cover with black mulch and continue irrigating, controlling the irrigation volume to 100% of field capacity and about 3 cm above the ground. Close all vents and seal the greenhouse in high temperature for 30 days. Based on the soil temperature monitoring probe data, ensure that the greenhouse temperature remains above 55 degrees Celsius for about 5 days.
[0087] Parameter range: Approximately 600 kg / mu of fresh tomato straw should be applied to ensure a carbon-nitrogen ratio of 17.5:1; the greenhouse should be sealed for 30 days, and the greenhouse temperature should be above 55 degrees Celsius after about 5 days; 100% saturated field water holding capacity.
[0088] Treatment 2 showed tylosin levels of 257.0 mg / kg, tetracycline levels of 31.5 mg / kg, sulfadiazine levels of 17.4 mg / kg, enrofloxacin levels of 268.8 ug / kg, estrone levels of 2233.5 ug / kg, and estriol levels of 48.1 ug / kg. The treatment was conducted during the high-temperature fallow period in greenhouses in July and August. After the previous crop was harvested, vegetable residues were thoroughly cleaned from the greenhouse, and the greenhouse film was repaired to ensure sealing and insulation. Then, fresh... Tomato stalks (approximately 600 kg / mu) were crushed and mixed with organic fertilizer (approximately 500 kg / mu) and spread. A rotary tiller was used to turn the soil to a depth of about 40 cm to ensure even tillage. A soil temperature monitoring probe was buried at a depth of 10 cm, and drip irrigation pipes were laid. The greenhouse was sealed with plastic film for drip irrigation for approximately 8.5 days. The greenhouse was then covered with black plastic film and irrigation continued, maintaining an irrigation volume of 100% of field capacity and about 3 cm above the ground. All vents were closed, and the greenhouse was sealed at high temperature for 30 days. Data from the soil temperature monitoring probe was then used for monitoring. The application rate of fresh tomato stalks was approximately 600 kg / mu, and the application rate of organic fertilizer was approximately 500 kg / mu, ensuring a carbon-to-nitrogen ratio of 14.5:1 and 100% saturated field capacity.
[0089] Treatment 3 showed tylosin levels of 359.7 mg / kg, tetracycline levels of 42.0 mg / kg, sulfadiazine levels of 17.2 mg / kg, enrofloxacin levels of 432.7 ug / kg, estrone levels of 4908.4 ug / kg, and estriol levels of 100.2 ug / kg. The treatment involved: during the high-temperature fallow period in greenhouses in July and August, after the previous crop was harvested, cleaning up vegetable residues inside the greenhouse and repairing the greenhouse film to ensure sealing and insulation; then, fresh tomato straw (approximately 6 kg) was added. After crushing approximately 750 kg / mu of fresh tomato straw and 10 kg / mu of high-temperature fermentation agent, mix and apply the mixture. Rotary tiller the soil to a depth of about 40 cm to ensure even tillage. Bury a soil temperature monitoring probe at a depth of 10 cm and lay drip irrigation pipes, sealing the greenhouse film for drip irrigation for about 8.5 days. Cover with black mulch and continue irrigation, controlling the irrigation volume to 100% of field capacity and about 3 cm above the ground. Close all vents and seal the greenhouse at high temperature for 30 days, monitoring the soil temperature probe data. The application rate of fresh tomato straw is approximately 600 kg / mu, wheat straw is approximately 750 kg / mu, and high-temperature fermentation agent is approximately 10 kg / mu, ensuring a carbon-to-nitrogen ratio of 29.5:1 and 100% saturated field capacity.
[0090] The value of untreated soil was 425.6 mg / kg.
[0091] As can be seen from the above experiments, all three treatments have a significant reduction effect on antibiotics (tylosin, tetracycline, sulfadiazine and enrofloxacin) and estrogens (estrone and estriol), among which treatment 1, which is the method of the present invention, has the most obvious reduction effect.
[0092] The above descriptions are merely some embodiments of the present invention. It should be noted that those skilled in the art can make other modifications and improvements without departing from the inventive concept of the present invention, and these all fall within the protection scope of the present invention.
Claims
1. A method for enhancing the reduction of antibiotics / estrogens in facility soil through high-temperature fumigation, characterized in that, Includes the following steps: S1. During the high-temperature fallow period in the greenhouse, after the previous crop is harvested, remove vegetable residues from the greenhouse, inspect the greenhouse equipment, and ensure the normal use and sealing of the greenhouse film for future use. S2. After crushing the tomato stalks, spread them, then use a rotary tiller to turn and till the soil to obtain furrows, including: In step S2, the tomato straw is crushed, mixed with organic fertilizer, and then spread, while maintaining a carbon-nitrogen ratio of 16-19:
1. S3. Bury the ground temperature monitoring probe in the cultivated ditch, then lay the drip irrigation pipe, and then carry out drip irrigation after sealing the greenhouse film; S4. Cover the soil inside the greenhouse with black plastic film and irrigate until the water level inside the greenhouse reaches a preset condition, including: The preset conditions mentioned in step S4 refer to controlling the irrigation amount to 100% of the field capacity and 2-4 cm above the ground. S5. After closing the ventilation vents of the greenhouse, perform high-temperature fumigation, including: In step S5, the high-temperature fumigation period is 25-35 days; In step S5, the temperature is maintained above 55°C for at least 4-6 days during the high-temperature fumigation period.
2. The method for enhancing antibiotic / estrogen reduction in high-temperature soil fumigation according to claim 1, characterized in that: In step S2, 550-650 kg of the tomato straw is spread per acre.
3. The method for enhancing antibiotic / estrogen reduction in high-temperature soil fumigation according to claim 1, characterized in that: Apply 450-550 kg of organic fertilizer per acre.
4. The method for enhancing antibiotic / estrogen reduction in high-temperature soil fumigation according to claim 1, characterized in that: In step S2, the rotary tiller turns the soil to a depth of 15-25cm.
5. The method for enhancing antibiotic / estrogen reduction in high-temperature soil fumigation according to claim 1, characterized in that: In step S3, the ground temperature monitoring probe is buried at a depth of 7.5-12.5cm in the cultivated ditch.
6. The method for enhancing antibiotic / estrogen reduction in high-temperature soil fumigation according to claim 1, characterized in that: In step S3, drip irrigation is carried out for 7-10 days after the greenhouse film is sealed.
Citation Information
Patent Citations
Method for synchronously reducing secondary salinization and antibiotic resistance genes of greenhouse soil
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CN115581164A