A traditional Chinese medicine composition for preventing and treating oviduct inflammation in laying hens and its application

Abstract

This invention provides a traditional Chinese medicine composition for preventing and treating oviduct inflammation in laying hens, belonging to the field of feed additive development technology. The traditional Chinese medicine composition of this invention comprises the following components by weight percentage: 40%-50% Scutellaria baicalensis extract, 5%-15% Astragalus membranaceus polysaccharide, 30%-40% Oregano extract, and 5%-15% Mentha haplocalyx extract. The synergistic effects of Scutellaria baicalensis, Astragalus membranaceus, Oregano, and Mentha haplocalyx in this invention exert antibacterial, anti-inflammatory, immune-enhancing, and symptom-relieving effects, thereby achieving the purpose of preventing and treating oviduct inflammation in laying hens. It also has the advantages of being natural, low in toxicity, having few side effects, and being less likely to induce drug resistance.

Description

Technical Field

[0001] This invention belongs to the field of feed additive development technology, and in particular relates to a traditional Chinese medicine composition for preventing and treating oviduct inflammation in laying hens and its application. Background Technology

[0002] With the rapid development of poultry farming, the scale of egg-laying hen farming is constantly expanding. However, oviduct inflammation is a common and serious disease in egg-laying hen farming. Oviduct inflammation not only affects the egg-laying performance of hens, reducing egg production rate and egg quality, but may also damage the reproductive system of hens, increasing farming costs and causing significant economic losses to farmers.

[0003] Currently, the prevention and treatment of oviduct inflammation in laying hens mainly relies on antibiotics. However, long-term use of antibiotics not only easily leads to drug resistance in pathogens but may also leave residues in eggs, posing a potential threat to food safety. Furthermore, antibiotic use may disrupt the gut microbiota balance in laying hens, affecting their overall health.

[0004] In this context, finding a safe, effective, and residue-free method for preventing and treating oviduct inflammation in laying hens is particularly important. Traditional Chinese medicine (TCM) has advantages such as being natural, low in toxicity, having few side effects, and being less likely to induce drug resistance, making it a promising candidate for animal disease prevention and treatment. Many TCM herbs possess properties such as clearing heat and detoxifying, promoting blood circulation and removing blood stasis, and antibacterial and anti-inflammatory effects, showing potential in alleviating oviduct inflammation in laying hens.

[0005] However, existing traditional Chinese medicine (TCM) prevention and treatment programs suffer from problems such as unreasonable formulations, insignificant efficacy, and unclear mechanisms of action. Therefore, developing a highly effective and safe TCM composition for oviduct inflammation in laying hens is of great significance for ensuring the healthy development of the laying hen farming industry. Summary of the Invention

[0006] In view of this, the purpose of the present invention is to provide a traditional Chinese medicine composition and its application for preventing and treating oviduct inflammation in laying hens, which can reduce the negative impact of pathogens infecting the oviducts of laying hens on egg production performance and ensure the number of eggs laid.

[0007] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0008] A traditional Chinese medicine composition for preventing and treating oviduct inflammation in laying hens, comprising the following components by weight percentage: 40%-50% Scutellaria baicalensis extract, 5%-15% Astragalus membranaceus polysaccharide, 30%-40% Oregano extract, and 5%-15% Mentha haplocalyx extract.

[0009] Preferably, the preparation method of the Scutellaria baicalensis extract includes: taking crude powder of Scutellaria baicalensis, adding distilled water at a material-to-liquid ratio of 1:4-6, heating and decocting for 3.5-4.5 hours, filtering, and concentrating the filtrate to 1 / 4-1 / 6 volume concentrate to obtain the Scutellaria baicalensis extract.

[0010] Preferably, the method for preparing the oregano extract includes: soaking oregano in water as a solvent at a temperature of 30-40°C for 20-28 hours, followed by distillation of the soaking solution at a temperature of 88-92°C to obtain the oregano extract.

[0011] Preferably, the method for preparing the peppermint extract includes: using water as a solvent, reflux extraction of peppermint at a temperature of 75-85℃ for 1-2 hours, filtration and concentration of the extract, addition of ethanol to the concentrate, removal of the precipitate after sufficient precipitation, recovery of ethanol from the supernatant to obtain peppermint extract.

[0012] Preferably, the extract is concentrated to 1 / 8-1 / 12 of its volume; the volume ratio of the concentrate to ethanol is 1:08-1.2, and the ethanol concentration is 85%-95%.

[0013] The present invention also provides the application of the above-mentioned traditional Chinese medicine composition in the preparation of daily feed for laying hens.

[0014] Preferably, the amount of the traditional Chinese medicine composition added accounts for 0.01%-0.05% of the daily feed weight of laying hens.

[0015] Preferably, the laying hen diet comprises the following components by weight percentage: corn 56.8%-61.5%, soybean meal 22.15%-25.1%, rapeseed meal 3.0%-5.0%, wheat bran 4.75%-6.12%, limestone powder 1.0%-2.0%, dicalcium phosphate 1.5%-3.0%, salt 0.8%-1.3%, premix 2.5%-4.0%, and traditional Chinese medicine composition 0.01%-0.05%.

[0016] This invention also provides the application of the above-mentioned traditional Chinese medicine composition in the preparation of a drug for treating oviduct inflammation in laying hens.

[0017] Preferably, the oviduct inflammation in the laying hen is caused by Escherichia coli, Salmonella, or Staphylococcus aureus.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] This invention provides a traditional Chinese medicine composition for preventing and treating oviduct inflammation in laying hens. It can reduce the negative impact of pathogen infection on egg production performance, ensuring egg quantity. It can also lower the concentrations of inflammatory factors IL-1β and TNF-α in the serum of laying hens after pathogen infection, while increasing the concentrations of anti-inflammatory factors IL-2 and IL-10. In this invention, Scutellaria baicalensis, Astragalus membranaceus, Oregano, and Mentha haplocalyx work synergistically to exert antibacterial, anti-inflammatory, immune-enhancing, and symptom-relieving effects, thereby achieving the purpose of preventing and treating oviduct inflammation in laying hens. Furthermore, it has the advantages of being natural, low in toxicity, having few side effects, and being less likely to induce drug resistance. Detailed Implementation

[0020] This invention provides a traditional Chinese medicine composition for preventing and treating oviduct inflammation in laying hens, comprising the following components by weight percentage: 40%-50% Scutellaria baicalensis extract, 5%-15% Astragalus membranaceus polysaccharide, 30%-40% Oregano extract, and 5%-15% Mentha haplocalyx extract; preferably 45% Scutellaria baicalensis extract, 10% Astragalus membranaceus polysaccharide, 35% Oregano extract, and 10% Mentha haplocalyx extract.

[0021] In this invention, a preferred method for preparing Scutellaria baicalensis extract includes: taking crude powder of Scutellaria baicalensis, adding distilled water at a material-to-liquid ratio of 1:4-6 (mass-volume ratio), more preferably 1:5, heating and decocting for 3.5-4.5 hours, more preferably 4 hours, filtering, concentrating the filtrate to 1 / 4-1 / 6 volume of concentrate, and further concentrating to 1 / 5 volume to obtain Scutellaria baicalensis extract. The Scutellaria baicalensis extract of this invention can clear internal heat toxins, reduce inflammatory responses, and exert anti-inflammatory and antibacterial effects in preventing and treating oviduct inflammation in laying hens.

[0022] In this invention, astragalus polysaccharides can enhance the immune function of laying hens, improve their resistance, promote the body's self-repair ability, and help resist inflammation.

[0023] In this invention, a preferred method for preparing oregano extract includes: soaking oregano in water as a solvent at a temperature of 30-40°C, more preferably 36°C, for 20-28 hours, more preferably 24 hours; subsequently, distilling the soaking solution at a temperature of 88-92°C, more preferably 90°C, to obtain oregano extract. The oregano extract of this invention possesses antibacterial, anti-inflammatory, and antioxidant properties, and can inhibit the growth of pathogens and alleviate inflammatory symptoms.

[0024] In this invention, a preferred method for preparing the *Mentha haplocalyx* extract includes: reflux extraction of *Mentha haplocalyx* using water as a solvent at a temperature of 75-85°C, more preferably 80°C, for 1-2 hours, more preferably 1.5 hours; filtration and concentration of the extract; addition of ethanol to the concentrate; removal of the precipitate after it has fully formed; and recovery of ethanol from the supernatant to obtain the *Mentha haplocalyx* extract. In this invention, the extract is preferably concentrated to 1 / 8-1 / 12 of its volume, more preferably to 1 / 10 of its volume; the volume ratio of the concentrate to ethanol is preferably 1:08-1.2, more preferably 1:1; and the ethanol concentration is preferably 85%-95% (mass concentration), more preferably 90% (mass concentration). The *Mentha haplocalyx* extract of this invention has antibacterial and anti-inflammatory effects, helping to alleviate discomfort caused by oviduct inflammation in laying hens, and synergistically exerts preventive and therapeutic effects with other components.

[0025] The components of the herbal composition of this invention work together to exert antibacterial, anti-inflammatory, immune-enhancing, and symptom-relieving effects, thereby achieving the purpose of preventing and treating oviduct inflammation in laying hens.

[0026] The present invention also provides the application of the above-mentioned traditional Chinese medicine composition in the preparation of laying hen diet, preferably the amount of traditional Chinese medicine composition added accounts for 0.01%-0.05% of the weight of laying hen diet, more preferably 0.02%.

[0027] In this invention, the preferred laying hen diet comprises the following components by weight percentage: corn 56.8%-61.5%, soybean meal 22.15%-25.1%, rapeseed meal 3.0%-5.0%, wheat bran 4.75%-6.12%, limestone powder 1.0%-2.0%, dicalcium phosphate 1.5%-3.0%, salt 0.8%-1.3%, premix 2.5%-4.0%, and traditional Chinese medicine composition 0.01%-0.05%; more preferably, corn 59.5%, soybean meal 23.78%, rapeseed meal 4.0%, wheat bran 5.3%, limestone powder 1.4%, dicalcium phosphate 2.0%, salt 1.0%, premix 3.0%, and traditional Chinese medicine composition 0.02%.

[0028] The present invention also provides the application of the above-mentioned traditional Chinese medicine composition in the preparation of a drug for treating oviduct inflammation in laying hens; the preferred drug includes the above-mentioned traditional Chinese medicine composition and excipients, and the content of the traditional Chinese medicine composition is 0.01%-99.99%; more preferably, the oviduct inflammation in laying hens is caused by Escherichia coli, Salmonella or Staphylococcus aureus.

[0029] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0030] Example 1

[0031] A traditional Chinese medicine composition for preventing and treating oviduct inflammation in laying hens comprises the following components by weight percentage: 45% Scutellaria baicalensis extract, 10% Astragalus membranaceus polysaccharide, 35% Oregano extract, and 10% Mentha haplocalyx extract. The preparation steps are as follows:

[0032] Extraction of Scutellaria baicalensis extract: Take the crude powder of Scutellaria baicalensis, add distilled water at a material-to-liquid ratio of 1:5 (mass-to-volume ratio), mix thoroughly, heat and decoct in a pot for 4 hours, filter, and concentrate to 1 / 5 volume to obtain Scutellaria baicalensis extract.

[0033] Astragalus polysaccharides were purchased directly from the market;

[0034] Extraction of oregano extract: oregano was soaked in water at 36°C for 24 hours, followed by distillation at 90°C. The filtrate was collected to obtain oregano extract.

[0035] Extraction of peppermint extract: Peppermint was extracted by reflux with water as solvent at 80℃ for 1.5 h. The extract was then filtered and concentrated to 1 / 10 of its volume. 90% ethanol was added to the concentrate at a volume ratio of 1:1. After the precipitate was fully formed, it was removed and the ethanol was recovered from the supernatant to obtain peppermint extract.

[0036] The traditional Chinese medicine composition is obtained by mixing Scutellaria baicalensis extract, Astragalus membranaceus polysaccharide, Oregano extract and Mentha haplocalyx extract in a certain mass ratio.

[0037] Example 2

[0038] A laying hen diet comprises the following ingredients by weight percentage: 59.5% corn, 23.78% soybean meal, 4.0% rapeseed meal, 5.3% wheat bran, 1.4% limestone powder, 2.0% dicalcium phosphate, 1.0% salt, 3.0% premix, and 0.02% of the herbal composition from Example 1.

[0039] Example 3

[0040] A laying hen diet comprises the following ingredients by weight percentage: 56.8% corn, 25.1% soybean meal, 5.0% rapeseed meal, 6.12% wheat bran, 2.0% limestone powder, 3.0% dicalcium phosphate, 1.3% salt, 4.0% premix, and 0.01% of the herbal composition from Example 1.

[0041] Example 4

[0042] A laying hen diet comprises the following ingredients by weight percentage: 61.5% corn, 22.15% soybean meal, 3.0% rapeseed meal, 4.75% wheat bran, 1.0% limestone powder, 1.5% dicalcium phosphate, 0.8% salt, 2.5% premix, and 0.05% of the herbal composition from Example 1.

[0043] Example 5

[0044] A laying hen diet comprises the following ingredients by weight percentage: 58.0% corn, 24.5% soybean meal, 4.2% rapeseed meal, 5.5% wheat bran, 1.8% limestone powder, 2.2% dicalcium phosphate, 1.2% salt, 3.2% premix, and 0.04% of the herbal composition from Example 1.

[0045] Example 6

[0046] A laying hen diet comprises the following ingredients by weight percentage: 60.0% corn, 24.0% soybean meal, 4.5% rapeseed meal, 5.0% wheat bran, 1.5% limestone powder, 2.5% dicalcium phosphate, 1.1% salt, 3.5% premix, and 0.03% of the herbal composition from Example 1.

[0047] Example 7

[0048] A traditional Chinese medicine composition for preventing and treating oviduct inflammation in laying hens differs from Example 1 in that:

[0049] Extraction of Scutellaria baicalensis extract: Take crude powder of Scutellaria baicalensis, add distilled water at a ratio of 1:6, mix thoroughly, heat and decoct in a pot for 4.5 hours, filter, and concentrate to 1 / 4 volume to obtain Scutellaria baicalensis extract.

[0050] Extraction of oregano extract: oregano was soaked in water at 40°C for 28 hours, followed by distillation at 92°C. The filtrate was collected to obtain oregano extract.

[0051] Extraction of peppermint extract: Peppermint was extracted by reflux at 85°C for 2 hours using water as solvent. The extract was then filtered and concentrated to 1 / 8 of its volume. 95% ethanol was added to the concentrate at a volume ratio of 1:1.2. After the precipitate had fully formed, it was removed, and the ethanol was recovered from the supernatant to obtain peppermint extract.

[0052] Example 8

[0053] A traditional Chinese medicine composition for preventing and treating oviduct inflammation in laying hens differs from Example 1 in that:

[0054] Extraction of Scutellaria baicalensis extract: Take crude powder of Scutellaria baicalensis, add distilled water at a ratio of 1:4, mix thoroughly, heat and decoct in a pot for 3.5 hours, filter, and concentrate to 1 / 6 volume to obtain Scutellaria baicalensis extract.

[0055] Extraction of oregano extract: oregano was soaked in water at 30°C for 20 hours, followed by distillation at 90°C. The filtrate was collected to obtain oregano extract.

[0056] Extraction of peppermint extract: Peppermint was extracted by reflux at 75°C for 2 hours using water as solvent. The extract was then filtered and concentrated to 1 / 10 of its volume. 90% ethanol was added to the concentrate at a volume ratio of 1:0.8. After the precipitate was fully formed, it was removed, and the ethanol was recovered from the supernatant to obtain peppermint extract.

[0057] Example 9

[0058] (1) Experimental Design:

[0059] Hy-Line Brown laying hens (500 days old) were selected for the experiment and purchased from Xuanhu Laying Chicken Farm in Anhui Province.

[0060] The pathogens used in the in vitro test were Escherichia coli and Staphylococcus aureus, in a ratio of 1:2 (V:V).

[0061] During the trial, 42 healthy Hy-Line Brown laying hens were randomly divided into 7 groups of 6 each: a blank control group (blank group), a laying hen oviduct inflammation model group (model group), a Scutellaria baicalensis extract group (200 mg / kg), a low-dose anti-inflammatory complex group (anti-low-group), a medium-dose anti-inflammatory complex group (anti-medium-group), a high-dose anti-inflammatory complex group (anti-high-group), and an amoxicillin group.

[0062] After 7 days of acclimatization, the Scutellaria baicalensis extract group (200 mg / kg), the low-, medium-, and high-dose anti-inflammatory complex groups, and the amoxicillin group were administered the corresponding supplementary drugs by gavage twice daily, morning and evening. The dosage of the anti-inflammatory complex in each group was 50 mg, 100 mg, and 200 mg / kg of feed, respectively. The amoxicillin group was administered the dosage according to the amoxicillin instructions, at a dose of 0.25 g / kg of feed. The blank control group and the model group were administered the same volume of physiological saline by gavage.

[0063] On day 14 of the experiment (14 days before modeling), except for the control group, the other groups were instilled with mixed bacterial solution through the cloaca into the oviduct for 7 consecutive days (7 days after modeling).

[0064] (2) Detection indicators and statistics:

[0065] (2.1) Egg production: Eggs are collected and recorded at regular intervals each day;

[0066] (2.2) Inflammatory factors in laying hens' serum: Blood samples were collected after the experiment, and IL-1β, TNF-α, IL-2 and IL-10 were detected by enzyme-linked immunosorbent assay (ELISA).

[0067] Analysis of variance was performed using SPSS 26.0.

[0068] (3) Experimental results:

[0069] As shown in Table 1, the egg production in the model group dropped sharply to 6 eggs after modeling, indicating that infection of the oviduct of laying hens by pathogens has a significant negative impact on egg production. The Scutellaria baicalensis extract group produced 36 eggs after modeling, an increase compared to the model group, but still lower than the control group and the amoxicillin group. The anti-high-group produced 43 eggs after modeling, second only to the control group, showing the best performance. In summary, the traditional Chinese medicine composition in Example 1 has a certain effect on increasing egg production, but the effect varies with different dosages. Compared to amoxicillin, the traditional Chinese medicine composition also has advantages such as being natural, low in toxicity, having fewer side effects, and being less likely to induce drug resistance.

[0070] Table 1. Effects of adding different doses of traditional Chinese medicine composition to the diet of laying hens on the number of eggs laid (in eggs).

[0071] Before molding 51 47 49 47 42 44 53 After molding 45 6 36 34 38 43 40

[0072] As shown in Table 2, oral administration of Scutellaria baicalensis extract and different doses of the traditional Chinese medicine (TCM) combination to laying hens reduced the concentrations of inflammatory factors IL-1β and TNF-α in serum after bacterial infection, while increasing the concentrations of anti-inflammatory factors IL-2 and IL-10. In the TCM combination treatment, the concentrations of anti-inflammatory factors in the serum of the anti-high-dose group were significantly higher than those in the Scutellaria baicalensis extract group. Comprehensive analysis indicates that adding the TCM combination to the diet can better prevent and treat oviductitis in laying hens.

[0073] Table 2. Effects of adding different doses of traditional Chinese medicine composition to the diet of laying hens on serum inflammatory factors after modeling (ng / L)

[0074]

[0075] In the table, in the same row, identical lowercase letters indicate no significant difference (P>0.05); different lowercase letters indicate significant difference (P<0.05).

[0076] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A traditional Chinese medicine composition for preventing and treating oviduct inflammation in laying hens, characterized in that, It is composed of the following ingredients by weight percentage: Scutellaria baicalensis extract 40%-50%, Astragalus membranaceus polysaccharide 5%-15%, Oregano extract 30%-40%, and Mentha haplocalyx extract 5%-15%; The preparation method of the Scutellaria baicalensis extract includes: taking crude powder of Scutellaria baicalensis, adding distilled water at a material-to-liquid ratio of 1:4-6, heating and decocting for 3.5-4.5 hours, filtering, and concentrating the filtrate to 1 / 4-1 / 6 volume concentrate to obtain the Scutellaria baicalensis extract. The method for preparing the oregano extract includes: soaking oregano in water as a solvent at a temperature of 30-40°C for 20-28 hours, followed by distillation of the soaking solution at a temperature of 88-92°C to obtain the oregano extract; The preparation method of the peppermint extract includes: using water as a solvent, reflux extraction of peppermint at a temperature of 75-85℃ for 1-2 hours, filtering and concentrating the extract to 1 / 8-1 / 12 volume concentrate; adding ethanol to the concentrate at a volume ratio of 1:08-1.2 and an ethanol concentration of 85%-95%; removing the precipitate after it has fully formed, recovering the ethanol from the supernatant to obtain the peppermint extract.

2. The application of the traditional Chinese medicine composition according to claim 1 in the preparation of laying hen diet feed.

3. The application according to claim 2, characterized in that, The amount of the traditional Chinese medicine composition added accounts for 0.01%-0.05% of the daily feed weight of laying hens.

4. The application according to claim 2, characterized in that, The laying hen diet comprises the following components by weight percentage: corn 56.8%-61.5%, soybean meal 22.15%-25.1%, rapeseed meal 3.0%-5.0%, wheat bran 4.75%-6.12%, limestone powder 1.0%-2.0%, dicalcium phosphate 1.5%-3.0%, salt 0.8%-1.3%, premix 2.5%-4.0%, and traditional Chinese medicine composition 0.01%-0.05%.

5. The use of the traditional Chinese medicine composition according to claim 1 in the preparation of a drug for treating oviduct inflammation in laying hens.

6. The application according to claim 5, characterized in that, The oviduct inflammation in laying hens is caused by Escherichia coli, Salmonella, or Staphylococcus aureus.

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