A plant protection method based on irradiation-associated microorganisms

Abstract

This invention discloses a plant protection method based on irradiation combined with microorganisms. The method includes the following steps: selecting and culturing microorganisms that maximally inhibit the growth of the nematode type affecting the plant; setting different dose rates and dose gradients, and sequentially irradiating, screening, and culturing the microorganisms according to these gradients to obtain a microbial population with good radiation resistance; inoculating the radiation-resistant microbial population into nematode-infected samples, and placing the treated samples in an irradiation area for short-term, high-dose-rate irradiation; culturing the irradiated samples to obtain protected plants. This invention effectively shortens the treatment time, significantly reduces the probability of nematodes developing radiation resistance, and greatly improves the efficiency of nematode suppression and killing.

Description

Technical Field

[0001] This invention belongs to the field of plant protection technology, and in particular relates to a plant protection method based on irradiation combined with microorganisms. Background Technology

[0002] Currently, in the field of plant protection, plant nematodes seriously affect the normal growth of plants. Therefore, many research methods have been proposed to suppress and kill plant nematodes.

[0003] Microbial inhibition is a conventional method for treating plant nematodes, primarily utilizing microorganisms to inhibit their reproduction and growth. Radiation irradiation, on the other hand, works by depositing energy into the nematodes using various types of rays. This energy disrupts the protein structure of the nematodes, inhibiting their reproduction and ultimately killing them. The more energy deposited, the stronger the inhibition. However, both methods have significant drawbacks. Microbial inhibition requires prolonged interaction between microorganisms and nematodes, resulting in relatively low efficiency. While radiation irradiation can inhibit and inactivate nematodes under high doses, the nematodes develop radiation resistance during the inactivation process, leading to a gradual decrease in the effectiveness of radiation irradiation. Summary of the Invention

[0004] The purpose of this invention is to provide a plant protection method based on irradiation combined with microorganisms, which aims to address the shortcomings of existing plant protection methods.

[0005] This invention is implemented as follows: a plant protection method based on irradiation combined with microorganisms, the method comprising the following steps:

[0006] (1) Select microorganisms that can inhibit the growth of the nematode that infest the plant to the greatest extent for cultivation;

[0007] (2) Set different dose rates and different dose gradients, and irradiate, screen and culture microorganisms in sequence according to the gradient settings, and finally obtain a microbial population with good radiation resistance.

[0008] (3) Inoculate a microbial population with good radiation resistance into the sample infected with nematodes, and place the treated sample in the radiation irradiation area for short-term, high-dose-rate irradiation treatment.

[0009] (4) The irradiated sample was cultured to obtain the protected plant.

[0010] Preferably, in step (1), the nematode type is one of Southern Root-Knot Nematode, Northern Root-Knot Nematode, or Elephant Ear Root-Knot Nematode, and the microorganism is one or more of Bacillus cereus, Microcystis thuringiensis, or Bacillus thuringiensis.

[0011] Preferably, in step (2), the irradiation source is one of a neutron source, a gamma source, an electron source, a proton source, or a heavy ion source.

[0012] Preferably, in step (2), after the final gradient of high dose rate and high dose conditions is completed, the surviving microorganisms are propagated and cultured for three generations to obtain a microbial population with good radiation resistance.

[0013] Preferably, in step (3), the sample is a plant seed or a plant plant.

[0014] Compared with the shortcomings and deficiencies of existing technologies, this invention has the following beneficial effects: Addressing the problem of low efficiency in inhibiting and killing nematodes by microorganisms, this invention introduces radiation irradiation technology to cultivate radiation-resistant microbial populations under irradiation conditions, reducing the impact of radiation irradiation on microbial survival and providing a good foundation for combining radiation irradiation technology with microbial action. This invention utilizes radiation-resistant microorganisms combined with radiation irradiation technology to jointly and concurrently inhibit and kill nematodes under high dose rate irradiation conditions, effectively shortening the action time, effectively reducing the probability of nematodes developing radiation resistance, and significantly improving the efficiency of nematode inhibition and killing. Attached Figure Description

[0015] Figure 1 This is a flowchart of the steps of the present invention;

[0016] Figure 2 The images show Codonopsis pilosula plants infected with northern root-knot nematodes (third from the right) and Codonopsis pilosula plants treated according to the present invention (third from the left). Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0018] This invention discloses a plant protection method based on irradiation-associated microorganisms, combined with... Figure 1 Understandably, this method includes the following steps:

[0019] (1) Select microorganisms that can inhibit the growth of the nematode that infest the plant to the greatest extent for cultivation;

[0020] Different types of nematodes infect different plants, requiring the selection of appropriate microorganisms for inhibition. Based on previous experiments, among the different types of microorganisms tested, Bacillus cereus, Microcystis thuringiensis, and Bacillus thuringiensis showed varying degrees of growth inhibition against southern root-knot nematodes, northern root-knot nematodes, or bean weevil root-knot nematodes. Therefore, these three microorganisms were selected for biocontrol experiments against nematodes.

[0021] Therefore, the first step is to select microorganisms that can inhibit the growth of the target nematode type to the greatest extent, and then prepare a suitable culture medium for the propagation and cultivation of the microbial population.

[0022] (2) Set different dose rates and different dose gradients, and irradiate, screen and culture microorganisms in sequence according to the gradient settings, and finally obtain a microbial population with good radiation resistance.

[0023] In step (2), the radiation source being irradiated is one of a neutron radiation source, a gamma radiation source, an electron radiation source, a proton radiation source, or a heavy ion radiation source.

[0024] The suppression and killing of nematodes using radiation irradiation mainly relies on the interaction between radiation and substances within the nematodes, causing energy to be deposited inside. This deposited energy then acts on the nematode's proteins, thereby killing the nematodes. Since radiation also affects microorganisms, when combining radiation irradiation with microbial treatment, it is first necessary to obtain a population of microorganisms with radiation resistance.

[0025] In step (2), the process of cultivating radiation-resistant microorganisms includes:

[0026] 1) Set different dose rates and dose gradients according to the radiation source conditions;

[0027] 2) Irradiate microorganisms under low dose rate and low dose conditions, screen the irradiated microorganisms, obtain surviving microorganisms, and culture the microorganisms using culture medium;

[0028] 3) Continuously increase the irradiation dose and dose rate, and repeatedly irradiate, screen and culture the microorganisms;

[0029] 4) Irradiation was carried out under high dose rate and high dose conditions to allow surviving microorganisms to multiply and be cultured;

[0030] 5) After three generations of propagation and culture, obtain a microbial population with radiation resistance.

[0031] (3) Inoculate a microbial population with good radiation resistance into the sample infected with nematodes, and place the treated sample in the radiation irradiation area for short-term, high-dose-rate irradiation treatment.

[0032] In step (3), a microbial population with good radiation resistance can be obtained through microbial cultivation. Therefore, in the process of combining radiation irradiation technology with microbial action to inhibit and kill nematodes, the microbial population can maintain high activity during irradiation, thus performing parallel inhibition and killing treatment on nematodes. The specific process is as follows:

[0033] 1) Select plant seeds or plants infected by nematodes as samples;

[0034] 2) Inoculate the radiation-resistant microbial population onto the sample for propagation;

[0035] 3) Place the treated sample in a high dose rate irradiation area for a short period of time.

[0036] (4) Cultivate the irradiated sample to obtain the protected plant;

[0037] In step (4), plant samples without nematode infestation are obtained by using radiation irradiation technology combined with microorganisms to inhibit and kill nematodes. The treated plant samples are then propagated and cultivated to obtain plants without nematode infestation, which can be used for later production applications to achieve plant protection.

[0038] The invention and its beneficial effects will be further illustrated below through specific examples:

[0039] A Co-60 gamma-ray source was selected as the radiation source, which emits characteristic gamma rays of 1.17 MeV and 1.33 MeV. Plants infested by nematodes were observed to identify the nematode type. For the northern root-knot nematode, a Bacillus thuringiensis population was selected, and culture medium was prepared for population culture.

[0040] In the Co-60 gamma source chamber, three dose rate points of 1 kGy / h, 5 kGy / h and 10 kGy / h were selected for irradiation from low dose rate to high dose rate, with different irradiation times set for each. The dose parameters for each irradiation are shown in Table 1.

[0041] Table 1 Irradiation parameters for each round

[0042]

[0043]

[0044] After each irradiation, the irradiated Bacillus thuringiensis were screened to obtain surviving Bacillus thuringiensis, which were then cultured in a culture medium. After the culture was completed, the next round of irradiation was carried out. After the 15th irradiation, the surviving Bacillus thuringiensis were cultured for three generations to obtain an irradiated Bacillus thuringiensis population.

[0045] Plants infected with northern root-knot nematodes were selected, and the number of nematodes on the plants was observed. The plants were then inoculated with a population of radiation-resistant Bacillus thuringiensis. The treated plants were then placed at a high dose rate of 10 kGy / h for short-term irradiation, with the irradiation time set to 5 h.

[0046] Figure 2 The figure shows a comparison between Codonopsis pilosula infected with the disease and Codonopsis pilosula treated according to the present invention. It can be seen from the figure that after Codonopsis pilosula was infected with the northern root-knot nematode, the root system showed weakening of the taproot, an increase in fibrous roots, and numerous root knots connecting to form fibrous root clusters. Figure 2 (Right third); After treatment, the roots of the Codonopsis pilosula plants showed strong taproots, few or no fibrous roots, and no root knots. Figure 2 (Third from the left). Therefore, by combining microbial treatment with gamma irradiation, observing the plant growth and the number of nematodes surviving in the plants after treatment, and finding no root knots in the plant roots and that the plant growth was consistent with that of healthy plants, the effectiveness of the irradiation-combined microbial treatment in killing root-knot nematodes and protecting the host plant was verified.

[0047] This invention addresses the problem of slow and inefficient microbial treatment of nematodes. Based on radiation irradiation technology, it specifically screens radiation-resistant microbial populations. By using radiation irradiation and radiation-resistant microorganisms to act on nematodes simultaneously, it can achieve highly efficient killing of nematodes in plants and highly efficient plant protection. The results are excellent, making it a high-performance plant protection method.

[0048] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A plant protection method based on irradiation-associated microorganisms, characterized in that, The method includes the following steps: (1) For the type of nematode that infests the plant, select microorganisms that can inhibit the growth of the nematode to the greatest extent and culture them; the type of nematode is one of the southern root-knot nematode, the northern root-knot nematode or the bean root-knot nematode, and the microorganism is one or more of the following: Bacillus cereus, sulfur-loving red oomycetes or Bacillus thuringiensis. (2) Set different dose rates and different dose gradients, and irradiate, screen and culture microorganisms in sequence according to the gradient settings to finally obtain a microbial population with good radiation resistance; the radiation source of the irradiation is one of neutron radiation source, gamma radiation source, electron radiation source or proton radiation source and heavy ion radiation source. Three dose rate points of 1 kGy / h, 5 kGy / h, and 10 kGy / h were selected for sequential irradiation from low to high dose rate; the radiation-resistant microbial cultivation process included: 1) Set different dose rates and dose gradients according to the radiation source conditions; 2) Irradiate microorganisms under low dose rate and low dose conditions, screen the irradiated microorganisms, obtain surviving microorganisms, and culture the microorganisms using culture medium; 3) Continuously increase the irradiation dose and dose rate, and repeatedly irradiate, screen, and culture the microorganisms; 4) Irradiation was carried out under high dose rate and high dose conditions to allow surviving microorganisms to multiply and be cultured; 5) After three generations of propagation and culture, a microbial population with radiation resistance was obtained; (3) Inoculate the radiation-resistant microbial population into the nematode-infected sample and place the treated sample in the radiation irradiation area for short-term, high-dose-rate irradiation treatment; specifically, place it at a high-dose-rate point of 10 kGy / h for short-term irradiation, and set the irradiation time to 5h. (4) The irradiated sample was cultured to obtain the protected plant.

2. The method as described in claim 1, characterized in that, In step (2), after the final gradient of high dose rate and high dose conditions is completed, the surviving microorganisms are propagated and cultured for three generations to obtain a microbial population with good radiation resistance.

3. The method as described in claim 1, characterized in that, In step (3), the sample is a plant seed or a plant plant.

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