A composition for promoting tomato graft healing and a preparation method and application thereof

Abstract

The application discloses a composition for promoting tomato graft healing and a preparation method and application thereof, and comprises the following raw materials in mass fraction: 0.1-0.5% of allicin, 0.1-0.3% of chitooligosaccharide, 0.1-0.2% of extract of poterium spicatum, 0.5-2% of lemon essential oil, 2-10% of aloe vera gel, 2-5% of ethanol, and the balance of water. The application can stimulate the active growth of protoplasm in cells, make the cells in the formation layer and parenchyma tissue vigorously split, form callus, and can induce new transport tissues to be generated, and can be applied to green and organic tomato production.

Description

Technical Field

[0001] This application relates to the field of tomato grafting management technology, and in particular to a composition that promotes tomato graft healing, its preparation method, and its application. Background Technology

[0002] Grafted tomato seedlings offer advantages such as overcoming continuous cropping obstacles, reducing disease incidence, increasing yield, improving quality, and enhancing drought resistance, leading to their widespread use in production. However, grafting is a technically challenging process. Without mature and scientific techniques and procedures, grafting wounds may not heal properly, affecting grafting success. Furthermore, in tomato grafting seedling cultivation, the presence of grafting wounds and the need for high humidity to promote healing often lead to fungal or bacterial infections in seedlings, impacting graft survival rates and seedling quality. Therefore, production requires a healing agent that can both promote wound healing and provide some disinfection and sterilization. Summary of the Invention

[0003] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is that the existing technology is highly subjective and difficult to assess the combined impact area. Therefore, the present invention proposes an evaluation method for artificial rain enhancement operations based on the index threshold method.

[0004] To achieve the above objectives, this invention provides a method for evaluating the effectiveness of artificial rain enhancement operations based on the index threshold method, specifically including the following steps:

[0005] This invention includes the following steps:

[0006] A composition for promoting graft healing in tomatoes comprises the following raw materials in the indicated weight percentages: allicin 0.1-0.5%, chitosan oligosaccharide 0.1%-0.3%, Lysimachia christinae aqueous extract 0.1-0.2%, lemon essential oil 0.5-2%, aloe vera gel 2-10%, ethanol 2-5%, and the balance being water.

[0007] A method for preparing a composition that promotes graft healing in tomatoes includes the following steps:

[0008] A. Fresh aloe vera leaves are crushed into a thin slurry using a high-speed grinder, then centrifuged and precipitated using a high-speed centrifuge, filtered to obtain aloe vera gel, and then water is added to prepare solution A.

[0009] B. Dissolve zeatin in a 70% ethanol solution to prepare solution D;

[0010] C. Dissolve lemon essential oil in a 70% ethanol solution to prepare solution E;

[0011] D. Dissolve allicin in water and stir well to obtain solution B. Dissolve chitosan oligosaccharide in solution B to obtain buffer solution C. Dissolve the water extract of *Lysimachia christinae* in buffer solution C to obtain solution F.

[0012] E. Mix solutions D, E, and F, and then mix them by ultrasonic vibration to obtain mixed solution G. Slowly add the obtained mixed solution G to solution A, and mix them evenly to obtain the original solution of the composition.

[0013] Further, the stock solution of the composition comprises the following raw materials in the indicated mass fractions: allicin 0.1-0.5%, chitosan oligosaccharide 0.1%-0.3%, Lysimachia christinae aqueous extract 0.1-0.2%, lemon essential oil 0.5-2%, aloe vera gel 2-10%, ethanol 2-5%, and the balance being water.

[0014] Furthermore, the preparation method of the Lysimachia christinae water extract includes extracting fresh Lysimachia christinae plants by boiling water for 30 minutes and then drying and evaporating them.

[0015] Furthermore, the method for preparing lemon essential oil includes cutting clean lemon peel into pieces, placing them in a distiller, adding water to completely soak the lemon peel, heating the distiller until steam rises, and distilling for 2-3 hours to extract and collect lemon essential oil.

[0016] The application of the composition for promoting the healing of tomato grafting wounds in plant grafting involves first soaking the cut end of the scion in the stock solution of the composition for 5-10 seconds during tomato tube grafting, then joining the scion to the cut end of the rootstock, and finally covering it with a tube to complete the grafting.

[0017] When grafting with a grafting clip, after the tomato scion and rootstock are joined, the grafting clip is attached, and then the undiluted composition is sprayed onto the wound. The undiluted composition is diluted 10 times with water and sprayed onto the entire grafted seedling.

[0018] Furthermore, the grafting method is to use a patch grafting method. Make an incision of about 45 degrees on the hypocotyl of the scion and the hypocotyl of the rootstock with a sharp blade. Soak the scion in the original solution of the composition for 10 seconds, with the soaking height so that the liquid level is higher than the incision. Place the scion on the rootstock and fix the scion and rootstock with a sleeve.

[0019] By adopting the above solution, the present invention has the following advantages:

[0020] This invention proposes a composition for promoting the healing of tomato grafting wounds, its preparation method, and its application. Zeatin in the composition is a natural cytokinin that stimulates active growth of intracellular protoplasm, causing vigorous cell division in the cambium and parenchyma tissues to form callus tissue. It can also induce the production of new vascular tissue, promoting the healing of the rootstock and scion into a unified whole. Lemon essential oil is a natural antioxidant that effectively reduces and inhibits the oxidation of phenolic substances at the wound site, thereby promoting wound healing and improving the survival rate of grafted tomato seedlings. Chitosan oligosaccharides and aloe vera gel provide abundant polysaccharides, vitamins, and other natural nutrients, which help the growth and differentiation of new cells between the rootstock and scion, promoting rapid healing of the grafting wound. Both lemon essential oil and aloe vera gel have moisturizing properties, helping to prevent moisture loss at the grafting wound site. Allicin, Lysimachia christinae extract, and aloe vera gel in the composition all have antibacterial effects, preventing harmful bacteria from invading through the grafting wound.

[0021] The main components of the composition provided by this invention are extracted or prepared from natural plant and animal materials, and can be applied to green and organic tomato production, which is in line with the technological innovation direction of green development. Attached Figure Description

[0022] Figure 1 This is an overall flowchart of the present invention; Detailed Implementation

[0023] The following describes embodiments of the present invention to make its technical content clearer and easier to understand. The present invention can be embodied in many different forms of embodiments, which are described exemplarily, and the scope of protection of the present invention is not limited to the embodiments mentioned herein.

[0024] Example 1

[0025] like Figure 1 As shown, this embodiment provides a composition for promoting the healing of grafted tomato wounds. The composition comprises the following raw materials in the indicated mass fractions: allicin 0.5%, chitosan oligosaccharide 0.3%, Lysimachia christinae aqueous extract 0.2%, lemon essential oil 2%, aloe vera gel 10%, ethanol 5%, and water 82%. The preparation method of this composition includes the following steps:

[0026] 1. Crush fresh aloe vera leaves into a thin slurry using a high-speed grinder, then centrifuge and precipitate the slurry using a high-speed centrifuge, filter the slurry to obtain aloe vera gel, and then add water to prepare solution A.

[0027] 2. Dissolve allicin in water and stir well to obtain solution B.

[0028] 3. Dissolve chitosan oligosaccharide in solution B to obtain buffer solution C.

[0029] 4. Dissolve zeatin (ZT) in 70% ethanol solution to prepare solution D.

[0030] 5. Dissolve lemon essential oil in 70% ethanol solution to prepare solution E.

[0031] Lemon essential oil: Cut clean lemon peel into small pieces, put them in a distiller, add an appropriate amount of water,

[0032] Soak the lemon peel completely, heat the still until steam rises, and distill for 2-3 hours to extract and collect the lemon essential oil.

[0033] 6. Dissolve the water extract of *Lysimachia christinae* in buffer solution C to obtain solution F.

[0034] Lysimachia christinae water extract: obtained by soaking fresh Lysimachia christinae plants in boiling water for 30 minutes and then drying and evaporating.

[0035] 7. Mix solutions D, E, and F, and then mix them by ultrasonic vibration to obtain mixed solution G.

[0036] 5. Slowly add the mixed solution G obtained in the above steps to solution A. After the two are mixed evenly, the stock solution of the composition for promoting the healing of grafted tomato wounds of the present invention is obtained.

[0037] The inventors conducted grafting experiments on the above composition, and the specific methods are as follows:

[0038] The tomato scion variety used in the experiment was 'Millennium', and the rootstock variety was 'Strong'. 32-cell seedling trays were used for sowing and seedling raising, and vegetable seedling substrate was used. Grafting was performed when the scion seedlings had two true leaves and the rootstock seedlings had 4-5 true leaves. The grafting method was used. First, a 45° incision was made on the hypocotyl of the scion and the hypocotyl of the rootstock with a sharp blade. The scion was then immersed in the stock solution of the grafting compound for 10 seconds, with the immersion level above the incision. The scion was then placed on the rootstock, and the scion and rootstock were securely fixed together with a sleeve. The healing of the grafting wound was observed 15 days after grafting. The grafting survival rate, blight incidence, and damping-off incidence were recorded. The xylem connectivity was determined using the fuchsin method.

[0039] Fuchsia method: Take seedlings of the same treatment, cut the base of the stem horizontally with a blade, and place them vertically in a beaker containing a 1% acidic fuchsin aqueous solution. After 4 hours, cut off the seedling above the graft union and weigh it. Put it in a mortar, add 5 mL of distilled water and grind it into a homogenate. Centrifuge at 12000 r / min for 6 min, take the supernatant and centrifuge again for 3 min. Use a spectrophotometer to measure the absorbance value A545 of the supernatant after the second centrifugation, and read the fuchsin content on the standard curve.

[0040] Example 2

[0041] This embodiment provides a composition for promoting the healing of grafted wounds in tomatoes, its preparation method, and its application. The composition and preparation method are the same as in Example 1. The difference from Example 1 is that a sleeve is not used, and the scion is not soaked in the undiluted composition. Instead, the scion is placed on the rootstock, and the scion is firmly fixed together with a grafting clip. The undiluted composition is then sprayed onto the wound site.

[0042] Example 3

[0043] This embodiment provides a composition for promoting the healing of grafted wounds in tomatoes, its preparation method, and its application. The composition and preparation method are the same as in Example 1. The difference from Example 1 is that a sleeve is not used, and the scion is not soaked in the original composition solution. Instead, the scion is placed on the rootstock, and the grafting points are tightly fixed together with grafting clips. Then, the grafted seedling is sprayed with a 10-fold diluted solution of the composition.

[0044] Comparative Example

[0045] Comparative Example 1

[0046] Unlike Example 1, after making incisions in the scion and rootstock, the scion is directly grafted onto the rootstock instead of being soaked in the original solution of the composition.

[0047] Comparative Example 2

[0048] Unlike Example 1, the scion was not soaked in the stock solution of the composition, but was soaked in clean water for 10 seconds.

[0049] Comparative Example 3

[0050] Unlike Example 2, after fixing the scion and rootstock with grafting clips, the undiluted composition was not sprayed on the wound area; instead, water was sprayed.

[0051] Comparative Example 4

[0052] Unlike Example 3, after fixing the scion and rootstock with grafting clips, the grafted seedlings were not sprayed with a 10-fold diluted solution of the composition, but instead sprayed with water.

[0053] Comparative Example 5

[0054] This application provides a comparative example of a composition for promoting the healing of grafted tomato wounds, its preparation method, and its application, which differs from Example 1 in that:

[0055] The composition comprises: 0.3% chitosan oligosaccharide, 0.2% Lysimachia christinae aqueous extract, 2% lemon essential oil, 10% aloe vera gel, 5% ethanol, and 82.5% water.

[0056] Comparative Example 6

[0057] This application provides a comparative example of a composition for promoting the healing of grafted tomato wounds, its preparation method, and its application, which differs from Example 1 in that:

[0058] The composition includes: 0.5% allicin, 0.3% chitosan oligosaccharide, 2% lemon essential oil, 10% aloe vera gel, 5% ethanol, and 82.2% water.

[0059] Comparative Example 7

[0060] This application provides a comparative example of a composition for promoting the healing of grafted tomato wounds, its preparation method, and its application, which differs from Example 1 in that:

[0061] The composition comprises: 0.5% allicin, 0.3% chitosan oligosaccharide, 0.2% Lysimachia christinae aqueous extract, 2% lemon essential oil, 5% ethanol, and 92% water.

[0062] Comparative Example 8

[0063] This application provides a comparative example of a composition for promoting the healing of grafted tomato wounds, its preparation method, and its application, which differs from Example 1 in that:

[0064] The composition comprises: 0.5% allicin, 0.2% Lysimachia christinae aqueous extract, 2% lemon essential oil, 10% aloe vera gel, 5% ethanol, and 82.3% water.

[0065] Comparative Example 9

[0066] This application provides a comparative example of a composition for promoting the healing of grafted tomato wounds, its preparation method, and its application, which differs from Example 1 in that:

[0067] The composition comprises: 0.5% allicin, 0.3% chitosan oligosaccharide, 0.2% Lysimachia christinae aqueous extract, 10% aloe vera gel, 5% ethanol, and 84.3% water.

[0068] Comparative Example 10

[0069] This application provides a comparative example of a composition for promoting the healing of grafted tomato wounds, its preparation method, and its application, which differs from Example 1 in that:

[0070] The composition comprises: 0.05% allicin, 0.3% chitosan oligosaccharide, 0.05% Lysimachia christinae aqueous extract, 10% aloe vera gel, 5% ethanol, and 84.6% water.

[0071] Comparative Example 11

[0072] This application provides a comparative example of a composition for promoting the healing of grafted tomato wounds, its preparation method, and its application, which differs from Example 1 in that:

[0073] The composition comprises: 0.5% allicin, 0.3% chitosan oligosaccharide, 0.2% Lysimachia christinae aqueous extract, 0.1% lemon essential oil, 1% aloe vera gel, 5% ethanol, and 92.9% water.

[0074] The experimental results (Table 1) show that, compared with Comparative Example 1 (without special treatment), all three treatments—Soaking the cuttings in the undiluted composition solution in Example 1, Spraying with the undiluted composition solution in Example 2, and Spraying with a 10-fold diluted solution in Example 3—significantly improved the survival rate of grafted tomato seedlings, significantly enhanced the xylem transport capacity of the grafted seedlings, and also significantly reduced the incidence of blight and damping-off. Compared with Comparative Examples 2-4 (treated with water), Examples 1-3 showed significantly higher survival rates and fuchsin content, and significantly lower incidences of blight and damping-off. The incidences of blight and damping-off in Examples 1-3 were significantly lower than those in Comparative Examples 5-7, indicating that allicin, *Lysimachia christinae* extract, and aloe vera gel all have certain antibacterial effects. The survival rates and fuchsin content in Examples 1-3 were significantly higher than those in Comparative Examples 7-9, indicating that chitosan oligosaccharide, lemon essential oil, and aloe vera gel all contribute to the healing of grafting wounds and improve grafting survival rates.

[0075] Table 1 Effects of different treatments on grafted tomato seedlings

[0076]

[0077]

[0078] Note: Different letters in the same column indicate significant differences (P<0.05).

[0079] In summary, the composition proposed in this invention for promoting the healing of tomato grafting wounds can effectively promote the healing of tomato grafting wounds and effectively prevent the invasion of pathogens, thereby improving the grafting survival rate and grafting quality.

[0080] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A composition for promoting graft healing in tomatoes, characterized in that, The raw materials include the following components by mass fraction: allicin 0.1-0.5%, chitosan oligosaccharide 0.1%-0.3%, Lysimachia christinae aqueous extract 0.1-0.2%, lemon essential oil 0.5-2%, aloe vera gel 2-10%, ethanol 2-5%, and the balance being water; Fresh aloe vera leaves were pulverized into a thin slurry using a high-speed grinder, then centrifuged and precipitated using a high-speed centrifuge, and finally filtered to obtain aloe vera gel. The preparation method of the water extract of Lysimachia christinae includes extracting fresh Lysimachia christinae plants by boiling water for 30 minutes and then drying and evaporating them. The method for preparing lemon essential oil includes cutting clean lemon peel into pieces, placing them in a distiller, adding water to completely soak the lemon peel, heating the distiller until steam rises, and distilling for 2-3 hours to extract and collect the lemon essential oil.

2. A method for preparing a composition that promotes graft healing in tomatoes, used to prepare the composition as described in claim 1, characterized in that, Includes the following steps: A. Fresh aloe vera leaves are crushed into a thin slurry using a high-speed grinder, then centrifuged and precipitated using a high-speed centrifuge, filtered to obtain aloe vera gel, and then water is added to prepare solution A. B. Dissolve zeatin in a 70% ethanol solution to prepare solution D; C. Dissolve lemon essential oil in a 70% ethanol solution to prepare solution E; D. Dissolve allicin in water and stir well to obtain solution B. Dissolve chitosan oligosaccharide in solution B to obtain buffer solution C. Dissolve the water extract of *Lysimachia christinae* in buffer solution C to obtain solution F. E. Mix solutions D, E, and F, and then mix them by ultrasonic vibration to obtain mixed solution G. Slowly add the obtained mixed solution G to solution A, and mix them evenly to obtain the original solution of the composition.

3. The method for preparing the composition for promoting graft healing in tomatoes as described in claim 2, characterized in that, The stock solution of the composition includes the following raw materials in the following mass fractions: allicin 0.1-0.5%, chitosan oligosaccharide 0.1%-0.3%, Lysimachia christinae aqueous extract 0.1-0.2%, lemon essential oil 0.5-2%, aloe vera gel 2-10%, ethanol 2-5%, and the balance being water.

4. The method for preparing the composition for promoting graft healing in tomatoes as described in claim 2, characterized in that, The preparation method of the water extract of Lysimachia christinae includes extracting fresh Lysimachia christinae plants by boiling water for 30 minutes and then drying and evaporating them.

5. The method for preparing the composition for promoting graft healing in tomatoes as described in claim 2, characterized in that, The method for preparing lemon essential oil includes cutting clean lemon peel into pieces, placing them in a distiller, adding water to completely soak the lemon peel, heating the distiller until steam rises, and distilling for 2-3 hours to extract and collect the lemon essential oil.

6. The application of the composition according to claim 1 for promoting the healing of tomato grafting wounds in plant grafting, characterized in that, When grafting tomatoes with a tube, first soak the cut end of the scion in the original solution of the composition for 5-10 seconds, then attach the scion to the cut end of the rootstock, and finally put on the tube to complete the grafting. When grafting with a grafting clip, after the tomato scion and rootstock are joined, the grafting clip is attached, and then the undiluted composition is sprayed onto the wound. The undiluted composition is diluted 10 times with water and sprayed onto the entire grafted seedling.

7. The application of the composition for promoting graft healing in tomato grafting as described in claim 6, characterized in that, Grafting is performed using the applicator method, where a 45-degree incision is made on the hypocotyl of the scion and the rootstock using a sharp blade. o Make a cut, immerse the scion in the stock solution of the composition for 10 seconds, with the immersion height so that the liquid level is above the cut, place the scion on the rootstock, and fix the scion and rootstock with a sleeve.

Images