A traditional Chinese medicine composition for treating canine parvovirus disease, an extract thereof, and a medicine comprising the extract

Abstract

The present application belongs to the field of animal medicine, and particularly relates to a traditional Chinese medicine composition for treating canine parvovirus disease, and also relates to a corresponding extract and a medicine containing the extract. The traditional Chinese medicine composition comprises, by weight, 10-35 parts of biota orientalis, 10-35 parts of spilanthes acmella, 10-35 parts of andrographis, 10-25 parts of sanguisorba officinalis, 10-25 parts of scutellaria baicalensis, 10-25 parts of pinellia ternata, 10-20 parts of pueraria, 10-15 parts of sophora flower, 10-15 parts of dried ginger, 5-15 parts of ginseng, and 5-15 parts of licorice. The medicine comprises, by weight percentage, 1%-3% of compound vitamin B, 5%-20% of beef enzymatic solution, 0.3% of preservative, 0.1% of sweetening agent, and the rest of the extract of the traditional Chinese medicine composition for treating canine parvovirus disease. The extract of the traditional Chinese medicine composition can be used for preparing a medicine oral liquid for treating canine parvovirus disease. The oral medicine is prepared through strict traditional Chinese medicine compounding and taste adjustment, has no side effect, has no drug residue, and achieves the purpose of safely and effectively curing canine parvovirus disease.

Description

Technical Field

[0001] This invention belongs to the field of veterinary medicine, specifically relating to a traditional Chinese medicine composition for treating canine parvovirus disease, as well as a corresponding extract and a drug containing the extract. Background Technology

[0002] Canine parvovirus disease (CPV) is an acute infectious disease caused by canine parvovirus infection in dogs. It is characterized by hemorrhagic enteritis and canines of all ages, sexes, and breeds are susceptible. The main sources of infection are infected and carrier dogs, who shed the virus through urine, feces, saliva, or vomit, easily contaminating food, water, and utensils. Young, unvaccinated puppies are most susceptible, and outbreaks within litters are common. Affected dogs are characterized by severe vomiting, hemorrhagic diarrhea, dehydration, and a significant decrease in white blood cell count. It is a common clinical disease, with a mortality rate of 50%–100% in infected puppies. The disease progresses rapidly and is one of the most serious and deadly infectious diseases affecting dogs.

[0003] Because this disease has a rapid onset and short course, it can lead to death in dogs if not treated promptly. There is currently no specific cure; treatment is mainly symptomatic, depending on the dog's condition. Generally, fluid replacement is the primary treatment, supplemented by hyperimmune serum or monoclonal antibody therapy, antibiotics, and interferon injections. Furthermore, the effectiveness of vaccines, hyperimmune serums, and monoclonal antibodies currently used to prevent and treat canine parvovirus varies, and the effectiveness of hyperimmune serums is declining year by year. Vaccines and antibodies have certain limitations, and various antibiotics used for adjuvant therapy have led to drug resistance and other problems. Therefore, developing novel, highly effective, and non-toxic traditional Chinese medicine compositions to combat canine parvovirus has become a new research topic. Summary of the Invention

[0004] In view of the problems and deficiencies in the existing technology, the present invention provides a traditional Chinese medicine composition for treating canine parvovirus disease, and also provides an extract prepared using the traditional Chinese medicine composition and a drug containing the extract.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] In a first aspect, the present invention provides a traditional Chinese medicine composition for treating canine parvovirus disease, comprising the following components by weight: 10-35 parts of Platycladus orientalis leaves, 10-35 parts of Portulaca oleracea, 10-35 parts of Andrographis paniculata, 10-25 parts of Sanguisorba officinalis, 10-25 parts of Scutellaria baicalensis, 10-25 parts of Pinellia ternata, 10-20 parts of Pueraria lobata, 10-15 parts of Sophora japonica flowers, 10-15 parts of dried ginger, 5-15 parts of ginseng, and 5-15 parts of licorice.

[0007] Preferably, by weight, it comprises the following components: 25-30 parts of Platycladus orientalis leaves, 25-30 parts of Portulaca oleracea, 25-30 parts of Andrographis paniculata, 15-20 parts of Sanguisorba officinalis, 15-20 parts of Scutellaria baicalensis, 15-20 parts of Pinellia ternata, 13-17 parts of Pueraria lobata, 10-15 parts of Sophora japonica flowers, 10-15 parts of dried ginger, 5-10 parts of ginseng, and 5-10 parts of licorice.

[0008] Secondly, the present invention provides an extract of a traditional Chinese medicine composition for treating canine parvovirus disease, comprising:

[0009] (A) Andrographis paniculata extract, which is an ethanol extract of Andrographis paniculata, with a weight-volume ratio of ethanol extract of Andrographis paniculata to Andrographis paniculata of 1ml:2g.

[0010] (B) Scutellaria baicalensis extract is a solution obtained by dissolving baicalin, the acid-precipitated product of Scutellaria baicalensis, in 8 times the concentration of 50V% ethanol solution and adjusting the pH value to neutral. The weight-volume ratio of this solution to Scutellaria baicalensis is 1ml:2g.

[0011] (C) Aqueous extracts of Platycladus orientalis leaves, Portulaca oleracea, Sanguisorba officinalis, Pinellia ternata, Pueraria lobata, Sophora japonica flowers, dried ginger, ginseng, and licorice, wherein the weight-volume ratio of the aqueous extracts to the corresponding medicinal ingredients is 1 ml: 2 g.

[0012] Add the extracts of Andrographis paniculata (component A) and Scutellaria baicalensis (component B) to the aqueous extract of component C, mix well, and adjust the pH to 5.5-6.0 to obtain the extract of the traditional Chinese medicine composition for treating canine parvovirus disease.

[0013] Preferably, component A, the Andrographis paniculata extract, is extracted by the following steps: Andrographis paniculata is placed in an 80% ethanol solution for maceration and extraction to obtain an maceration solution; the maceration solution is concentrated under reduced pressure to 5% to 10% of its original volume, then decolorized, filtered, and the filtrate is concentrated under reduced pressure to obtain the Andrographis paniculata extract.

[0014] Furthermore, the specific process of the maceration extraction is as follows: the Andrographis paniculata is macerated in an 8-10 times ethanol solution for 24 hours, then removed and macerated in an 6-8 times ethanol solution for 24 hours, and the two maceration solutions are combined.

[0015] Furthermore, the decolorization process involves heating and refluxing with activated carbon for 30 minutes, with the amount of activated carbon being 10% to 20% of the weight of Andrographis paniculata.

[0016] Furthermore, the specific filtration process is as follows: filter and collect the filtrate and filter residue, wash the filter residue twice with anhydrous ethanol at 50-70°C and collect the washing liquid, and combine the filtrate and washing liquid.

[0017] Preferably, the Scutellaria baicalensis extract of component B is prepared by the following steps:

[0018] Scutellaria baicalensis was decocted with 8-10 times its volume of water for 2 hours and then filtered. The dregs were decocted with 6-8 times its volume of water for 2 hours and then filtered. The filtrates from the two decoctions were combined and concentrated under reduced pressure to 5%-10% of the original volume. The filtrate was then collected and the pH was adjusted to 1-2 with acid. The mixture was kept at 80℃ for 30 minutes and then cooled to precipitate crystals, yielding crude baicalin.

[0019] Dissolve crude baicalin in 8 times its volume of water, adjust the pH to 7, filter, collect the filtrate, add acid to the filtrate to adjust the pH to 1-2, keep warm at 50℃ for 30 min, cool to precipitate crystals, and obtain baicalin.

[0020] Baicalin was dissolved in 8 times its volume of 50% ethanol solution, the pH was adjusted to 7, filtered, the filtrate was collected, and concentrated under reduced pressure to obtain scutellaria extract.

[0021] Furthermore, the pH adjustment to 1-2 using acid is achieved using hydrochloric acid solution.

[0022] Furthermore, the pH value was adjusted to 7 using a 40% sodium hydroxide solution.

[0023] Preferably, the aqueous extract of component C is prepared by the following steps: taking arborvitae leaves, purslane, burnet root, pinellia, kudzu root, sophora japonica flower, dried ginger, ginseng, and licorice root by weight ratio, adding 8-10 times the amount of water and decocting for 2 hours, then filtering; adding 6-8 times the amount of water to the residue and decocting for 2 hours, then filtering; combining the filtrates from the two decoctions, concentrating under reduced pressure to obtain the aqueous extract of component C.

[0024] Thirdly, the present invention provides a drug for treating canine parvovirus disease, the drug being an oral liquid dosage form comprising an extract of the traditional Chinese medicine composition for treating canine parvovirus disease described in the second aspect and pharmaceutically acceptable excipients.

[0025] Preferably, the drug comprises the following weight percentages: 1%–3% compound vitamin B, 5%–20% beef enzymatic hydrolysate, 0.3% preservative, 0.1% sweetener, and the remainder being an extract of the traditional Chinese medicine composition for treating canine parvovirus disease as described in the second aspect.

[0026] Furthermore, the preservative is at least one of sodium benzoate, potassium sorbate, and sodium paraben, and the sweetener is at least one of aspartame and acesulfame potassium.

[0027] Preferably, the drug is prepared by the following method: adding compound vitamin B, beef enzymatic hydrolysate, preservative and sweetener to the extract of the traditional Chinese medicine composition for treating canine parvovirus disease described in the second aspect, mixing thoroughly, and then centrifuging and irradiating to sterilize, thereby obtaining the drug for treating canine parvovirus disease.

[0028] Preferably, the centrifugation speed is 10,000 rpm and the time is 10 min.

[0029] All components in the traditional Chinese medicine composition of this invention can be purchased commercially available products.

[0030] In the formula,

[0031] Arborvitae leaves: The principal herb, bitter, astringent, and cold in nature. It enters the lung, liver, and spleen meridians. It has the functions of cooling the blood and stopping bleeding, resolving phlegm and relieving cough, and promoting hair growth and darkening hair. It is used for hematemesis, epistaxis, hemoptysis, hematochezia, metrorrhagia, cough due to lung heat, hair loss due to blood heat, and premature graying of hair.

[0032] Purslane: The principal herb, sour and cold in nature. It enters the liver and large intestine meridians. It has the functions of clearing heat and detoxifying, cooling blood and stopping bleeding, and stopping dysentery. It is used for dysentery caused by heat toxins, carbuncles and boils, eczema, erysipelas, snake and insect bites, hematochezia, hemorrhoidal bleeding, and metrorrhagia.

[0033] Andrographis paniculata: the principal herb, bitter and cold in nature, enters the heart, lung, bladder, and large intestine meridians. It has the functions of clearing heat and detoxifying, reducing swelling and relieving pain; it is mainly used to treat colds with fever, damp-heat diarrhea, carbuncles, boils, and other skin infections.

[0034] Sanguisorba officinalis: An assistant herb, it is sour, bitter, astringent, and slightly cold in nature, and enters the liver and large intestine meridians. It has the functions of cooling the blood and detoxifying, stopping bleeding and astringing sores; it is mainly used to treat bloody dysentery, epistaxis, uterine bleeding, boils, carbuncles, etc. It can enhance and protect the hematopoietic system, and increase peripheral blood leukocytes, neutrophils, and platelets. It improves bone marrow microcirculation and regulates and improves the body's immune function.

[0035] Scutellaria baicalensis: An assistant herb, bitter and cold in nature. It enters the lung, gallbladder, spleen, large intestine, and small intestine meridians. Its functions include clearing heat and drying dampness, purging fire and detoxifying, stopping bleeding, and calming the fetus. It is used to treat damp-heat syndrome, summer-heat syndrome, chest tightness and nausea, damp-heat fullness in the abdomen, diarrhea, etc.

[0036] Pinellia ternata: An assistant herb, pungent and warm in nature; toxic. It enters the spleen, stomach, and lung meridians. Its functions include drying dampness and resolving phlegm, suppressing nausea and vomiting, and dissipating lumps and nodules. It is used for damp phlegm, cold phlegm, cough with excessive phlegm, phlegm-dampness causing dizziness and palpitations, wind-phlegm causing dizziness, phlegm-induced syncope causing headache, vomiting, and nausea.

[0037] Kudzu root: An adjuvant herb, sweet, pungent, and cool in nature. It enters the spleen, stomach, and lung meridians. It has the functions of relieving muscle tension and reducing fever, promoting body fluid production and quenching thirst, promoting rash eruption, and raising yang to stop diarrhea. It is mainly used to treat exogenous fever with headache, stiff neck and back pain, thirst, diabetes, incomplete measles eruption, dysentery, and diarrhea.

[0038] Sophora japonica flower: An adjuvant medicine, bitter and slightly cold. It enters the liver and large intestine meridians. It has the functions of cooling the blood and stopping bleeding, clearing liver heat and purging fire. It is used for hematochezia, hemorrhoidal bleeding, bloody dysentery, metrorrhagia, hematemesis, epistaxis, red eyes due to liver heat, headache, and dizziness.

[0039] Dried ginger: An adjuvant herb, pungent and hot. It enters the spleen, stomach, kidney, heart, and lung meridians. It has the functions of warming the middle jiao and dispelling cold, restoring yang and unblocking the meridians, and warming the lungs and resolving phlegm. It is mainly used to treat cold pain in the epigastrium and abdomen, vomiting and diarrhea, cold limbs and weak pulse, and cough with cold phlegm.

[0040] Ginseng: A medicinal herb, sweet and slightly bitter, slightly warm in nature. It enters the spleen, lung, heart, and kidney meridians. It has the functions of greatly replenishing vital energy, restoring the pulse and consolidating the body, tonifying the spleen and lungs, generating fluids and nourishing blood, calming the mind and improving intelligence. It is mainly used to treat weakness and impending collapse, cold limbs and weak pulse, spleen deficiency with poor appetite, lung deficiency with cough and asthma, thirst due to fluid depletion, internal heat and thirst, and deficiency of qi and blood.

[0041] Licorice: An adjuvant herb, sweet and neutral in nature. It enters the heart, lung, spleen, and stomach meridians. Its functions include tonifying the spleen and replenishing qi, clearing heat and detoxifying, resolving phlegm and relieving cough, relieving spasms and pain, and harmonizing other herbs. It is primarily used to treat weakness of the spleen and stomach, fatigue, palpitations, shortness of breath, cough with excessive phlegm, and spasms and pain in the abdomen and limbs.

[0042] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0043] 1. The drug formulation of this invention is used for clearing heat and detoxifying, stopping bleeding and cooling the blood, and relieving nausea and vomiting. Among them, *Platycladus orientalis* leaves (cooling the blood and stopping bleeding), *Portulaca oleracea* (clearing heat and detoxifying, cooling the blood and stopping bleeding), and *Andrographis paniculata* (clearing heat and detoxifying) are the principal herbs, working synergistically to exert the main therapeutic effect. *Sanguisorba officinalis* (cooling the blood and stopping bleeding) and *Scutellaria baicalensis* (clearing heat and drying dampness, purging fire and detoxifying) are the assistant herbs, assisting the principal herbs in enhancing their effects of clearing heat and detoxifying, cooling the blood and stopping bleeding. *Pinellia ternata* (drying dampness and resolving phlegm, relieving nausea and vomiting), *Pueraria lobata* (relieving muscle tension and reducing fever), *Sophora japonica* (cooling the blood and stopping bleeding), and dried ginger (warming the middle and dispelling cold) are the adjuvant herbs. *Pinellia ternata* and dried ginger are used to treat vomiting symptoms, *Sophora japonica* assists the principal and assistant herbs in exerting their effects of cooling the blood and stopping bleeding, and *Pueraria lobata* has the effect of raising yang. *Ginseng* (tonifying vital energy) and *Glycyrrhiza uralensis* (harmonizing the herbs) are the guiding herbs. *Ginseng* enhances the overall efficacy, and *Glycyrrhiza uralensis* harmonizes the herbs, helping them to exert a synergistic effect.

[0044] 2. The drug of the present invention has significant effects in animal experiments. In the experimental cases, compared with the Western medicine group, the combination of traditional Chinese and Western medicine can significantly improve the cure rate of canine parvovirus disease, shorten the course of the disease, and reduce the number of days for the affected dogs to stop bloody diarrhea and vomiting. This shows that the oral liquid of the present invention has a certain effect on the treatment of canine parvovirus disease.

[0045] 3. The oral medication of this invention has undergone strict formulation of traditional Chinese medicine and taste adjustment, has no side effects and no drug residues, and achieves the goal of safely and effectively curing canine parvovirus disease. Attached Figure Description

[0046] Figure 1 Image showing the results of a colloidal gold test strip that showed a positive result for canine parvovirus in a dog.

[0047] Figure 2 This is a picture of the feces of a dog suffering from canine parvovirus and exhibiting bloody diarrhea. Detailed Implementation

[0048] The following embodiments are only for further elaboration of the present invention. It should be noted that all techniques and scientific terms used in this invention, unless otherwise stated, have the same meaning as those in the technical field to which this invention pertains. Experimental methods in the following embodiments that do not specify specific conditions all employ conventional techniques in this technical field or follow the conditions recommended by the manufacturer; reagents or instruments whose manufacturers are not specified are all commercially available conventional products.

[0049] To make the objectives, technical solutions, and advantages of this invention clearer and more explicit, the invention is further described in detail below through embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0050] Example 1

[0051] The extract of the traditional Chinese medicine composition for treating canine parvovirus disease was obtained from the different weight parts of the traditional Chinese medicine composition shown in Table 1:

[0052] Table 1. Traditional Chinese medicine compositions in different weight parts

[0053] Arborvitae leaves 30 copies 10 copies 35 copies purslane 30 copies 10 copies 35 copies Andrographis paniculata 25 copies 10 copies 35 copies Burnt Sanguisorba officinalis 20 copies 10 copies 25 copies Scutellaria baicalensis 20 copies 10 copies 25 copies Pinellia 20 copies 10 copies 25 copies Kudzu root 15 copies 10 copies 20 copies locust flowers 12 copies 10 copies 15 copies Dried ginger 12 copies 10 copies 15 copies Ginseng 10 copies 5 copies 15 copies licorice 10 copies 5 copies 15 copies

[0054] The traditional Chinese medicine compositions in Table 1 were extracted using the following method to prepare the corresponding extracts:

[0055] (1) Andrographis paniculata was soaked in 10 times the concentration of 80% ethanol solution for 24 hours, filtered, and then soaked in 8 times the concentration of 80% ethanol solution for 24 hours. The two soaking solutions were combined and the soaking solution was concentrated under reduced pressure to 5% of the original volume. Then, it was decolorized by heating with activated carbon under reflux for 30 minutes. The amount of activated carbon was 15% of the mass of Andrographis paniculata. After decolorization, it was filtered and the filtrate and residue were collected. The residue was washed twice with anhydrous ethanol at 60℃ and the washing liquid was collected. The filtrate and washing liquid were mixed and concentrated under reduced pressure to obtain Andrographis paniculata extract. The ratio of Andrographis paniculata extract to Andrographis paniculata was 1 ml: 2 g.

[0056] (2) Scutellaria baicalensis was decocted in 10 times the amount of water for 2 hours, filtered, and the residue was decocted in 8 times the amount of water for 2 hours, filtered, and the filtrates from the two decoctions were combined. The filtrates were concentrated under reduced pressure to 5% of the original volume, then filtered and the filtrate was collected. Hydrochloric acid solution was added to the filtrate to adjust the pH to 1.5. The filtrate was kept at 80℃ for 30 minutes and then cooled to precipitate crystals, yielding crude baicalin. The crude baicalin was dissolved in 8 times the amount of water, and the pH was adjusted to 7 using a 40% sodium hydroxide solution. The filtrate was collected, and hydrochloric acid solution was added to the filtrate to adjust the pH to 1.5. The filtrate was kept at 50℃ for 30 minutes and then cooled to precipitate crystals, yielding baicalin. Baicalin was added to 8 times the amount of 50% ethanol solution, and the pH was adjusted to 7 using a 40% sodium hydroxide solution. The filtrate was collected, filtered, and concentrated under reduced pressure to obtain Scutellaria baicalensis extract. The ratio of Scutellaria baicalensis extract to Scutellaria baicalensis was 1 ml: 2 g.

[0057] (3) Mix the leaves of Platycladus orientalis, Portulaca oleracea, Sanguisorba officinalis, Pinellia ternata, Pueraria lobata, Sophora japonica, dried ginger, ginseng, and licorice to obtain a mixture of Chinese medicines. Add 10 times the amount of water to the mixture of Chinese medicines and decoct for 2 hours, then filter. Add 8 times the amount of water to the dregs and decoct for 2 hours, then filter. Combine the filtrates from the two decoctions and concentrate under reduced pressure to obtain a mixed extract. The ratio of the mixed extract to the mixture of Chinese medicines is 1 ml: 2 g. Add the extract of Andrographis paniculata obtained in step (1) and the extract of Scutellaria baicalensis obtained in step (2) to the mixed extract and mix well. Adjust the pH value to 5.5 to obtain the extract of the Chinese medicine composition for treating canine parvovirus disease. The compositions corresponding to those in Table 1 are labeled as extract 1, extract 2, and extract 3, respectively.

[0058] Example 2

[0059] Referring to the composition listed in Table 2, the oral liquid formulation of the drug for treating canine parvovirus disease is prepared using the following method:

[0060] Add compound vitamin B, beef enzymatic hydrolysate, preservative and sweetener to the extract, mix thoroughly, then centrifuge at 10,000 rpm for 10 minutes. After centrifugation, sterilize by irradiation to obtain the drug for treating canine parvovirus disease.

[0061] Table 2 shows the preparation of oral liquids with different weight percentages.

[0062] Extract Extract 1, 87.6% Extract 2, 76.6% Extract 3, 93.6% Complex Vitamin B 2％ 3％ 1％ Beef hydrolysate 10％ 20％ 5％ preservative 0.3％ 0.3％ 0.3％ sweeteners 0.1％ 0.1％ 0.1％

[0063] In Table 2, the compound vitamin B is an aqueous solution containing 2 mg of vitamin B1, 0.5 mg of vitamin B2, 0.3 mg of vitamin B6, 3 mg of nicotinamide, and 0.4 mg of sodium dextropantothenate per ml.

[0064] To verify the therapeutic effect of drugs for canine parvovirus disease, the following animal experiments were conducted.

[0065] Experimental Example 1:

[0066] 1. Experimental subjects and grouping

[0067] Dogs (all small breeds, under 12 months old, and confirmed cases of CPV infection) admitted to multiple animal hospitals in Henan Province (Zhengzhou, Xingyang, Nanyang, Luoyang, etc.) between January 2022 and June 2024 were selected. On the day of admission, their main clinical manifestations were mild diarrhea and vomiting, without myocarditis symptoms. These dogs were divided into two groups for treatment: a Western medicine group (n=20 dogs) and a combined traditional Chinese and Western medicine group (n=20 dogs).

[0068] 2. Experimental Design

[0069] Western medicine group: Intravenous infusion: 0.9% normal saline (20mL / kg) + hemostatic agent (0.2mL / kg) + 50% glucose (1mL / kg), 5% glucose (20mL / kg) + omeprazole (2mg / kg), once or twice a day; subcutaneous injection: ampicillin sodium (20mg / kg), canine parvovirus monoclonal antibody (1ml / kg), once a day.

[0070] The combined Chinese and Western medicine group: Intravenous infusion: 0.9% normal saline (20mL / kg) + hemostatic agent (0.2mL / kg) + 50% glucose (1mL / kg), 5% glucose (20mL / kg) + omeprazole (2mg / kg), 1-2 times a day; Subcutaneous injection: ampicillin sodium (20mg / kg), canine parvovirus monoclonal antibody (1ml / kg), once a day; Oral administration: oral solution of sample 1 (≤5kg, 5ml each time; 5-10kg, 10ml each time), twice a day.

[0071] 3. Experimental Indicators

[0072] The cure rate (cure rate (%) = number of cured animals (animals) ÷ number of cases (animals) × 100%), average treatment days, cessation of bloody diarrhea, and cessation of vomiting were statistically analyzed for the experimental animals in the Western medicine group and the combined Chinese and Western medicine group.

[0073] 4. Experimental Results

[0074] The cure rate and average treatment days of the experimental animals in Sample 1 are shown in Table 3.

[0075] Table 3. Results of cure rate and average treatment days for experimental animals in Sample 1.

[0076] Western medicine group 20 15 75％ 6.33±0.62 Chinese and Western medicine combined group 20 18 90％ 5.33±0.69

[0077] As shown in Table 3, the cure rate for canine parvovirus infection in the Western medicine group was 75%, while the cure rate in the combined Western and Traditional Chinese Medicine group (with the addition of oral Chinese medicine) was 90%. It can be seen that the course of disease in the combined Western and Traditional Chinese Medicine group was shorter than that in the Western medicine group, and there was a significant difference in the average number of treatment days between the two groups.

[0078] The results of stopping bloody diarrhea in the experimental animals of Sample 1 are shown in Table 4.

[0079] Table 4 Results of cessation of bloody diarrhea in experimental animals of Sample 1

[0080]

[0081] Table 4 shows that, comparing the number of days it took for bloody diarrhea to stop in the Western medicine group and the combined Western and Traditional Chinese Medicine group, the number of cases where bloody diarrhea stopped in 1-2 days was 0% in the Western medicine group and 11.1% in the combined Western and Traditional Chinese Medicine group; the number of cases where bloody diarrhea stopped in 3-4 days was 46.7% in the Western medicine group and 61.1% in the combined Western and Traditional Chinese Medicine group; the number of cases where bloody diarrhea stopped in 5-6 days was 53.3% in the Western medicine group and 27.8% in the combined Western and Traditional Chinese Medicine group; the average number of days it took for bloody diarrhea to stop in the Western medicine group was 4.80 days, while it was 3.83 days in the combined Western and Traditional Chinese Medicine group. It can be seen that there is a significant difference in the number of days it took for bloody diarrhea to stop between the Western medicine group and the combined Western and Traditional Chinese Medicine group (P<0.05).

[0082] The results of cessation of vomiting in the experimental animals of Sample 1 are shown in Table 5.

[0083] Table 5 Results of cessation of vomiting in experimental animals of Sample 1

[0084]

[0085] Table 5 shows that, comparing the number of days it took for vomiting to stop in the Western medicine group and the combined Western and Traditional Chinese Medicine group, the number of cases where vomiting stopped in 1-2 days was 0% in the Western medicine group and 11.1% in the combined Western and Traditional Chinese Medicine group; the number of cases where vomiting stopped in 3-4 days was 53.3% in the Western medicine group and 77.8% in the combined Western and Traditional Chinese Medicine group; the number of cases where vomiting stopped in 5-6 days was 46.7% in the Western medicine group and 11.1% in the combined Western and Traditional Chinese Medicine group; the average number of days it took for vomiting to stop in the Western medicine group was 4.53 days, while it was 3.67 days in the combined Western and Traditional Chinese Medicine group. It can be seen that there is a significant difference in the number of days for vomiting to stop between the Western medicine group and the combined Western and Traditional Chinese Medicine group (P<0.05).

[0086] Clinical trial data show that, compared with the Western medicine group, the combined treatment of traditional Chinese and Western medicine can significantly improve the cure rate of canine parvovirus disease, shorten the course of the disease, and reduce the number of days for affected dogs to stop bloody diarrhea and vomiting. This indicates that the oral liquid sample 1 prepared in Example 2 has a certain effect on the treatment of canine parvovirus disease.

[0087] Experimental Example 2:

[0088] 1. Experimental subjects and grouping

[0089] Five animals were included in the Western medicine group (from pet hospitals in Zhengzhou and Nanyang): Teddy Qiuqiu, 2 months old, 1 kg, male, unvaccinated, temperature 38.4℃, complained of mild vomiting after being purchased from the market about three days ago, and tested positive for canine parvovirus using a colloidal gold test strip. Pomeranian Dantart, 3 months old, 1.2 kg, female, one-dose vaccinated, temperature 38.2℃, complained of mild diarrhea after being taken out for play recently, and tested positive for canine parvovirus using a colloidal gold test strip. Bichon Frise Doudou, 2 months old, 1.1 kg, female, unvaccinated, temperature 38.3℃, complained of recent lethargy and mild vomiting and diarrhea, and tested positive for canine parvovirus using a colloidal gold test strip. Corgi Xiaoqi, 1.5 months old, 0.9 kg, male, unvaccinated, body temperature 38.1℃, chief complaint of recent lethargy, diarrhea and vomiting, brought to the vet for examination, canine parvovirus colloidal gold test strip was positive. Teddy mixed breed, Doubao, 2 months old.

[0090] 1.2kg, male, unvaccinated, body temperature 38.3℃. The patient complained of poor appetite recently, suspected of overeating, and noticed slight vomiting, so they came to the hospital for examination. The canine parvovirus colloidal gold test strip was positive.

[0091] Five animals (from pet hospitals in Xingyang and Luoyang) were included in the combined Chinese and Western medicine group: Mango (Teddy Bear), 3 months old, 2 kg, male, one-dose vaccine, body temperature 38.3℃, complained of recent mild vomiting, and tested positive for canine parvovirus using a colloidal gold test strip. Xiao Mei (Teddy Bear), 2 months old, 1.1 kg, female, unvaccinated, body temperature 38.4℃, complained of recent mild vomiting, and tested positive for canine parvovirus using a colloidal gold test strip. Liu Liu (Pomeranian), 1.5 months old, 0.8 kg, male, unvaccinated, body temperature 38.0℃, complained of recent mild diarrhea, and tested positive for canine parvovirus using a colloidal gold test strip. Xiong Er (Bichon Frise), 2 months old, 1.2 kg, male, unvaccinated, body temperature 38.4℃, complained of recent mild vomiting, and tested positive for canine parvovirus using a colloidal gold test strip. Pudding, a 2-month-old, 1.5kg female, unvaccinated, with a body temperature of 38.4℃, complained of mild diarrhea recently and was brought to the hospital for examination. The canine parvovirus colloidal gold test strip was positive.

[0092] The results of the colloidal gold test strip test for canine parvovirus in the above experimental animals are as follows: Figure 1 As shown, the stool of a dog with bloody dysentery is like... Figure 2 As shown.

[0093] 2. Experimental Design

[0094] Western medicine group: Intravenous infusion: 0.9% normal saline (20mL / kg) + hemostatic agent (0.2mL / kg) + 50% glucose (1mL / kg), 5% glucose (20mL / kg) + omeprazole (2mg / kg), once or twice a day; subcutaneous injection: ampicillin sodium (20mg / kg), canine parvovirus monoclonal antibody (1ml / kg), once a day.

[0095] The combined Chinese and Western medicine group: Intravenous infusion: 0.9% normal saline (20mL / kg) + hemostatic agent (0.2mL / kg) + 50% glucose (1mL / kg), 5% glucose (20mL / kg) + omeprazole (2mg / kg), 1-2 times a day; Subcutaneous injection: ampicillin sodium (20mg / kg), canine parvovirus monoclonal antibody (1ml / kg), once a day; Oral administration: oral solution of the drug prepared in Example 2 (≤5kg, 5ml each time; 5-10kg, 10ml each time), twice a day. The sample numbers of the specific oral drugs for dogs are shown in Table 6.

[0096] 3. Experimental Indicators

[0097] The 7-day blood routine data of experimental animals in the Western medicine group and the combined Chinese and Western medicine group were statistically analyzed.

[0098] 4. Experimental Results

[0099] The blood routine data of the experimental animals in Experiment Example 2 after 7 days are shown in Table 6.

[0100] Table 6. Blood routine data of experimental animals in Experiment Example 2 over 7 days.

[0101]

[0102]

[0103]

[0104]

[0105] As shown in Table 6, in the Western medicine group, the following dogs recovered and were discharged: Teddy Qiuqiu (4 days of treatment stopped bloody diarrhea and vomiting, blood routine returned to normal range on day 6), Pomeranian Egg Tart (4 days of treatment stopped bloody diarrhea and vomiting, blood routine returned to normal range on day 5), Bichon Frise Doudou (4 days of treatment stopped bloody diarrhea and vomiting, blood routine returned to normal range on day 6), Corgi Xiaoqi (5 days of treatment stopped vomiting, 6 days of treatment stopped bloody diarrhea, blood routine returned to normal range on day 7), and Teddy Chuan Doubao (4 days of treatment stopped vomiting, 6 days of treatment stopped bloody diarrhea, blood routine returned to normal range on day 7), and was discharged after 7 days of treatment.

[0106] In the combined Chinese and Western medicine group, the following dogs were treated: Teddy Mango, whose bloody diarrhea and vomiting stopped after 2 days of treatment and whose blood routine returned to normal on the 5th day; Teddy Xiaomei, whose vomiting and bloody diarrhea stopped after 2 days of treatment and whose blood routine returned to normal on the 5th day; Pomeranian Liuliu, whose vomiting and bloody diarrhea stopped after 4 days of treatment and whose blood routine returned to normal on the 6th day; Bichon Frise Xiong Er, whose vomiting and bloody diarrhea stopped after 3 days of treatment and whose blood routine returned to normal on the 6th day; and Rural Dog Pudding, whose vomiting and bloody diarrhea stopped after 4 days of treatment and whose blood routine returned to normal on the 5th day; and Rural Dog Pudding, whose vomiting and bloody diarrhea stopped after 4 days of treatment and whose blood routine returned to normal on the 7th day.

[0107] In summary, in the Western medicine group, vomiting stopped on average in 4.2 days, bloody diarrhea stopped on average in 4.8 days, and blood routine tests returned to normal on average in 6.2 days. In the combined Western and traditional Chinese medicine group, vomiting stopped on average in 3.0 days, bloody diarrhea stopped on average in 3.0 days, and blood routine tests returned to normal on average in 5.4 days. This demonstrates that the oral liquid samples 1-3 prepared in Example 2 can shorten the time to stop vomiting and bloody diarrhea, as well as the time to restore blood routine tests to normal.

[0108] This invention effectively overcomes the shortcomings of the prior art and has high industrial application value. The above embodiments are intended to illustrate the substantive content of this invention, but are not intended to limit the scope of protection of this invention. Those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this invention without departing from the essence and scope of protection of this invention.

Claims

1. A traditional Chinese medicine composition for treating canine parvovirus disease, characterized in that, It is made from the following ingredients in parts by weight: 10-35 parts of Platycladus orientalis leaves, 10-35 parts of Portulaca oleracea, 10-35 parts of Andrographis paniculata, 10-25 parts of Sanguisorba officinalis, 10-25 parts of Scutellaria baicalensis, 10-25 parts of Pinellia ternata, 10-20 parts of Pueraria lobata, 10-15 parts of Sophora japonica flowers, 10-15 parts of dried ginger, 5-15 parts of ginseng, and 5-15 parts of licorice.

2. The traditional Chinese medicine composition for treating canine parvovirus disease as described in claim 1, characterized in that, It is made from the following ingredients in parts by weight: 25-30 parts of Platycladus orientalis leaves, 25-30 parts of Portulaca oleracea, 25-30 parts of Andrographis paniculata, 15-20 parts of Sanguisorba officinalis, 15-20 parts of Scutellaria baicalensis, 15-20 parts of Pinellia ternata, 13-17 parts of Pueraria lobata, 10-15 parts of Sophora japonica flowers, 10-15 parts of dried ginger, 5-10 parts of ginseng, and 5-10 parts of licorice.

3. The extract of the traditional Chinese medicine composition for treating canine parvovirus disease according to claim 1 or 2, characterized in that, (A) Andrographis paniculata extract, which is an ethanol extract of Andrographis paniculata, with a weight-volume ratio of ethanol extract of Andrographis paniculata to Andrographis paniculata of 1ml:2g; (B) Scutellaria baicalensis extract is a solution obtained by dissolving baicalin, the acid-precipitated product of Scutellaria baicalensis, in 8 times the concentration of 50 V% ethanol solution and adjusting the pH value to neutral. The weight-volume ratio of this solution to Scutellaria baicalensis is 1 ml: 2 g. (C) Aqueous extracts of Platycladus orientalis leaves, Portulaca oleracea, Sanguisorba officinalis, Pinellia ternata, Pueraria lobata, Sophora japonica flowers, dried ginger, ginseng, and licorice, wherein the weight-volume ratio of the aqueous extract to the corresponding medicinal ingredients is 1 ml: 2 g. Add the extracts of Andrographis paniculata (component A) and Scutellaria baicalensis (component B) to the aqueous extract of component C, mix well, and adjust the pH to 5.5-6.0 to obtain the extract of the traditional Chinese medicine composition for treating canine parvovirus disease.

4. The extract of the traditional Chinese medicine composition for treating canine parvovirus disease as described in claim 3, characterized in that, The component A, Andrographis paniculata extract, is extracted by the following steps: Andrographis paniculata is placed in an 80% ethanol solution for maceration and extraction to obtain an maceration solution; the maceration solution is concentrated under reduced pressure to 5% to 10% of its original volume, then decolorized, filtered, and the filtrate is concentrated under reduced pressure to obtain the Andrographis paniculata extract.

5. The extract of the traditional Chinese medicine composition for treating canine parvovirus disease as described in claim 4, characterized in that, The decolorization process involves heating and refluxing with activated carbon for 30 minutes, with the amount of activated carbon being 10% to 20% of the weight of Andrographis paniculata.

6. The extract of the traditional Chinese medicine composition for treating canine parvovirus disease as described in claim 3, characterized in that, The Scutellaria baicalensis extract of component B is prepared by the following steps: Scutellaria baicalensis was decocted with 8-10 times the amount of water for 2 hours and filtered. The dregs were decocted with 6-8 times the amount of water for 2 hours and filtered. The filtrates from the two decoctions were combined and concentrated under reduced pressure to 5%-10% of the original volume. The filtrate was then collected and acid was added to adjust the pH to 1-2. The mixture was kept at 80℃ for 30 minutes and then cooled to precipitate crystals, yielding crude baicalin. Dissolve crude baicalin in 8 times its volume of water, adjust the pH to 7, filter, collect the filtrate, add acid to the filtrate to adjust the pH to 1-2, keep warm at 50℃ for 30 min, cool to precipitate crystals, and obtain baicalin. Baicalin was dissolved in 8 times its volume of 50% ethanol solution, the pH was adjusted to 7, the mixture was filtered, the filtrate was collected, and the extract was concentrated under reduced pressure to obtain baicalin extract.

7. The extract of the traditional Chinese medicine composition for treating canine parvovirus disease as described in claim 3, characterized in that, The aqueous extract of component C is prepared by the following steps: take arborvitae leaves, purslane, sanguisorba officinalis, pinellia ternata, kudzu root, sophora japonica flower, dried ginger, ginseng, and licorice root by weight ratio, add 8-10 times the amount of water and decoct for 2 hours, then filter; add 6-8 times the amount of water to the dregs and decoct for 2 hours, then filter; combine the filtrates from the two decoctions, concentrate under reduced pressure, and obtain the aqueous extract of component C.

8. A drug for treating canine parvovirus disease, characterized in that, The drug is an oral liquid formulation, made from an extract of the traditional Chinese medicine composition for treating canine parvovirus disease as described in any one of claims 3-7 and pharmaceutically acceptable excipients.

9. The medicament for treating canine parvovirus disease as described in claim 8, characterized in that, The weight percentage composition of the drug is as follows: compound vitamin B 1%–3%, beef enzymatic hydrolysate 5%–20%, preservative 0.3%, sweetener 0.1%, and the remainder is an extract of a traditional Chinese medicine composition for treating canine parvovirus disease.

10. The medicament for treating canine parvovirus disease as described in claim 9, characterized in that, The drug is prepared by the following method: compound vitamin B, beef enzymatic hydrolysate, preservative, and sweetener are added to the extract of the traditional Chinese medicine composition for treating canine parvovirus disease, mixed well, centrifuged to obtain the supernatant, and sterilized by irradiation to obtain the drug for treating canine parvovirus disease.

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