Traditional Chinese medicine composition for livestock and poultry antiviral and application

Abstract

This invention provides a traditional Chinese medicine composition for antiviral treatment of livestock and poultry, and its application, relating to the field of traditional Chinese medicine pharmaceutical technology for livestock and poultry. The traditional Chinese medicine composition provided by this invention is used to prepare drugs for the prevention or treatment of viral diseases in livestock and poultry. The traditional Chinese medicine composition comprises 10-30 parts of Magnolia officinalis, 10-30 parts of Taraxacum mongolicum, 10-30 parts of Dioscorea bulbifera, 10-30 parts of Dioscorea bulbifera, 1-10 parts of processed Aconitum carmichaelii, 10-30 parts of Commelina communis, 10-30 parts of Akebia trifoliata, 10-30 parts of Alisma plantago-aquatica, 10-30 parts of Ophiopogon japonicus, and 10-30 parts of Adenophora stricta. The traditional Chinese medicine composition of this invention can improve the immune organ index, enhance the body's immunity, and improve the body's disease resistance. It has significant therapeutic effects on a variety of viral diseases, such as avian influenza, infectious bursal disease, Newcastle disease, gout in goslings, porcine circovirus disease, transmissible gastroenteritis, epidemic diarrhea, rotavirus infection, bovine viral diarrhea, rabbit viral hemorrhagic disease, canine coronavirus diarrhea, feline panleukopenia, and mink viral enteritis, reducing mortality and improving production performance.

Description

Technical Field

[0001] This invention relates to the field of veterinary traditional Chinese medicine pharmaceutical technology, specifically to traditional Chinese medicine compositions and applications for antiviral treatment of livestock and poultry. Background Technology

[0002] In recent years, the high incidence of viral diseases in livestock, poultry, and fur-bearing animals has caused huge losses to the livestock industry. This category of diseases is a collective term for a group of illnesses caused by viral infections in livestock, poultry, and fur-bearing animals, including Newcastle disease, avian influenza, swine influenza, porcine reproductive and respiratory syndrome (PRRS), porcine circovirus disease, adenovirus infection, African swine fever, epidemic diarrhea, rabbit viral hemorrhagic disease, canine coronavirus diarrhea, feline panleukopenia, and mink viral enteritis, among others. These diseases are highly contagious, and most lack specific treatments. In practice, vaccination is the primary means of prevention and control. However, there are problems such as the lack of mature vaccines for some viral diseases, the tendency for viruses to mutate, and the incomplete protection and limited effectiveness of existing vaccines.

[0003] Due to their safety, low toxicity, environmental friendliness, and residue-free characteristics, traditional Chinese medicine (TCM) has been widely used by researchers to prevent and treat viral diseases in livestock, poultry, and fur-bearing animals. TCM possesses a complete theoretical system and unique advantages in the prevention and treatment of viral diseases. Numerous studies have reported significant efficacy of TCM in the prevention and early-to-mid-stage treatment of viral diseases. TCM theory emphasizes the interaction between viruses and the body, and TCM possesses comprehensive effects such as inhibiting viral replication, reducing tissue damage caused by viruses, and regulating immune function, thus becoming a hot topic of research for scholars both domestically and internationally in recent years. Summary of the Invention

[0004] (a) Technical problems to be solved

[0005] This invention provides a traditional Chinese medicine composition for antiviral treatment of livestock and poultry. The composition has significant antiviral and immune-enhancing effects and is effective in treating and preventing various viral diseases in livestock and poultry.

[0006] (II) Technical Solution

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] This invention provides a traditional Chinese medicine composition for antiviral treatment of livestock and poultry, the composition comprising the following raw materials in parts by weight: 10-30 parts Magnolia officinalis, 10-30 parts Taraxacum mongolicum, 10-30 parts Dioscorea bulbifera, 10-30 parts Dioscorea bulbifera, 1-10 parts processed Aconitum carmichaelii, 10-30 parts Commelina communis, 10-30 parts Akebia trifoliata, 10-30 parts Alisma plantago-aquatica, 10-30 parts Ophiopogon japonicus, and 10-30 parts Adenophora stricta.

[0009] Furthermore, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 10-20 parts Magnolia officinalis, 10-20 parts Taraxacum mongolicum, 15-25 parts Dioscorea bulbifera, 15-25 parts Dioscorea bulbifera, 1-10 parts processed Aconitum carmichaelii, 15-25 parts Commelina communis, 10-20 parts Akebia trifoliata, 10-20 parts Alisma plantago-aquatica, 10-20 parts Ophiopogon japonicus, and 10-20 parts Adenophora stricta.

[0010] Furthermore, the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 16 parts Magnolia officinalis, 14 parts Taraxacum mongolicum, 20 parts Dioscorea bulbifera, 18 parts Dioscorea bulbifera, 6 parts processed Aconitum carmichaelii, 20 parts Commelina communis, 16 parts Akebia trifoliata, 14 parts Alisma plantago-aquatica, 14 parts Ophiopogon japonicus, and 14 parts Adenophora stricta.

[0011] The traditional Chinese medicine composition provided by this invention is used to prepare drugs for the prevention and treatment of viral diseases in livestock and poultry. The traditional Chinese medicine composition can be made into oral preparations acceptable to animals, including but not limited to: granules, powders, extracts, pills, freeze-dried powders, and oral liquids.

[0012] Magnolia officinalis: It tastes bitter and pungent, and is warm in nature. It enters the spleen, stomach, lung, and large intestine meridians. It has the effects of drying dampness and eliminating phlegm, and lowering qi and relieving fullness. It is used for symptoms such as dampness stagnation injuring the middle jiao, epigastric fullness and vomiting and diarrhea, food stagnation and qi stagnation, abdominal distension and constipation, and phlegm-dampness cough.

[0013] Dandelion: It tastes bitter and sweet, is cold in nature, and enters the liver and stomach meridians; it has the effects of clearing heat and detoxifying, reducing swelling and dissipating nodules, and promoting diuresis and relieving strangury; it is used for boils and carbuncles, mastitis, scrofula, red eyes, sore throat, lung abscess, intestinal abscess, damp-heat jaundice, and painful urination due to heat; it contains effective components such as flavonoids, coumarins, phenolic acids, terpenes, sterols and polysaccharides, and clinical studies have shown that it has antibacterial, antioxidant, anti-inflammatory, anti-tumor and gastrointestinal protective pharmacological effects.

[0014] Yellow yam: It tastes bitter and pungent, and is cool in nature. It enters the liver, stomach, heart, and lung meridians. It has the effects of detoxifying and reducing swelling, resolving phlegm and dissipating nodules, cooling blood and stopping bleeding. It is used for goiter, lymph node tuberculosis, sore throat, hematemesis, hemoptysis, pertussis, cancer, etc.

[0015] Baiyaozi (Radix Dioscoreae): It tastes bitter and is cold in nature. It enters the spleen, lung, and kidney meridians. It has the effects of clearing heat and detoxifying, cooling blood and stopping bleeding, dispersing blood stasis and reducing swelling. It is used for acute hepatitis, bacterial dysentery, acute appendicitis, stomach pain, internal bleeding, snake bites, etc.

[0016] Prepared aconite: pungent, sweet, very hot and toxic, it enters the heart, kidney and spleen meridians; it has the functions of restoring yang and rescuing from collapse, tonifying fire and assisting yang, and dispelling wind and cold evil; it can be used for collapse due to yang deficiency and weak and cold pulse, and has the function of restoring yang and rescuing from collapse; externally, it can warm kidney yang, and can treat impotence, as well as infertility due to cold uterus, cold pain in the heart and abdomen, cold vomiting and diarrhea caused by cold evil stagnation in the stomach and intestines, and edema caused by excessive cold; it can also treat exogenous diseases due to yang deficiency and arthralgia due to cold and dampness.

[0017] Commelina communis: It has a sweet and bland taste, is cold in nature, and enters the lung, stomach, and small intestine meridians; it has the effects of clearing heat and purging fire, detoxifying, and promoting diuresis and reducing swelling; it is used for colds with fever, fever with thirst, sore throat, edema with scanty urine, painful urination, carbuncles and boils.

[0018] Akebia trifoliata: bitter in taste, cold in nature, enters the heart, small intestine and bladder meridians; it has the effects of promoting urination and relieving strangury, clearing the heart and relieving irritability, relieving dysmenorrhea and promoting lactation; it is used for strangury, edema, irritability and red urine, oral ulcers, amenorrhea and insufficient lactation, damp-heat arthralgia and other symptoms.

[0019] Alisma plantago-aquatica: It is cold in nature, sweet and bland in taste, and enters the kidney and bladder meridians. It has the effects of promoting diuresis and removing dampness, clearing heat, resolving turbidity and regulating lipids. It can be used for symptoms such as difficulty urinating, abdominal distension in water, diarrhea and scanty urine. It mainly contains chemical components such as triterpenoids and sesquiterpenoids, which have pharmacological effects such as enhancing immunity and anti-inflammation.

[0020] Ophiopogon japonicus: It tastes sweet and slightly bitter, and is slightly cold in nature. It enters the heart, lung, and stomach meridians. It has the effects of nourishing yin and promoting body fluid, moistening the lungs and relieving cough. The main chemical components of Ophiopogon japonicus include steroidal saponins, isoflavones, polysaccharides, etc., which have pharmacological effects such as lowering blood sugar, protecting the cardiovascular system, enhancing immunity, anti-skin aging, anti-inflammation, and anti-tumor.

[0021] Adenophora stricta: It tastes sweet and slightly bitter, and is slightly cold in nature. It enters the lung and stomach meridians. It has the effects of nourishing yin and clearing heat, moistening the lungs and resolving phlegm, and benefiting the stomach and promoting body fluids. It is often used for chronic cough due to yin deficiency, consumptive cough with bloody sputum, dry cough with little sputum, sore throat due to deficiency heat, and thirst due to fluid depletion.

[0022] Viral diseases fall under the category of epidemic diseases in Traditional Chinese Medicine (TCM), and their cause is the invasion of pathogenic factors. Therefore, dandelion, which is cold in nature and bitter in taste, is used as the principal herb for its effects of clearing heat, detoxifying, and healing sores. Magnolia officinalis and processed Aconitum carmichaelii are used as assistant herbs to dry and eliminate phlegm and expel wind-dampness. Dampness is the root of the disease, and when it combines with heat, it becomes damp-heat. Therefore, herbs such as Dioscorea bulbifera, Dioscorea opposita, and Commelina communis are added to provide support and clear heat and detoxify, while herbs such as Akebia trifoliata and Alisma plantago-aquatica are added to promote diuresis and clear heat. Damp-heat accumulates in the lungs, causing the lungs to fight against external pathogens and disrupt their function of dispersing and descending qi. Furthermore, dysfunction of the spleen and stomach can exacerbate the lungs' inability to disperse and descend qi. Therefore, herbs such as Ophiopogon japonicus and Adenophora stricta are added to provide support and nourish yin, moisten the lungs, benefit the stomach, and promote the production of body fluids to assist in the treatment.

[0023] (III) Beneficial Effects

[0024] This invention provides a traditional Chinese medicine composition for antiviral treatment of livestock, poultry, and fur-bearing animals. The composition comprises Magnolia officinalis, Taraxacum mongolicum, Dioscorea bulbifera, Dioscorea bulbifera, processed Aconitum carmichaelii, Commelina communis, Akebia trifoliata, Alisma plantago-aquatica, Ophiopogon japonicus, and Adenophora stricta, possessing the effects of clearing heat and detoxifying, removing dampness and heat, and promoting body fluid production and moistening the lungs. This traditional Chinese medicine composition exhibits significant therapeutic effects against various viral diseases, including avian influenza, infectious bursal disease, porcine circovirus disease, Newcastle disease, transmissible gastroenteritis, epidemic diarrhea, viral diarrhea, rotavirus infection, rabbit viral hemorrhagic disease, and canine coronavirus diarrhea. It reduces mortality, significantly improves the symptoms of diseased livestock, poultry, and fur-bearing animals, and enhances production performance. This traditional Chinese medicine composition also enhances the body's immunity, improves disease resistance, and has significant antiviral effects. Attached Figure Description

[0025] Figure 1 The bursal index of each experimental group of chickens was calculated. Compared with the blank control group, *p<0.05, **p<0.01.

[0026] Figure 2 The thymus index of each experimental group of chickens was compared with that of the blank control group. *p<0.05, **p<0.01.

[0027] Figure 3 The spleen index of each experimental chicken group was compared with that of the blank control group. *p<0.05, **p<0.01. Detailed Implementation

[0028] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0029] Example 1

[0030] A traditional Chinese medicine composition for antiviral treatment of livestock and poultry, the composition comprising the following raw materials in parts by weight: 16 parts Magnolia officinalis, 14 parts Taraxacum mongolicum, 20 parts Dioscorea bulbifera, 18 parts Dioscorea bulbifera, 6 parts processed Aconitum carmichaelii, 20 parts Commelina communis, 16 parts Akebia trifoliata, 14 parts Alisma plantago-aquatica, 14 parts Ophiopogon japonicus, and 14 parts Adenophora stricta.

[0031] The traditional Chinese medicine composition can be formulated into oral preparations acceptable to animals, including but not limited to: granules, powders, extracts, pills, freeze-dried powders, and oral liquids.

[0032] Example 2

[0033] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 10 parts Magnolia officinalis, 10 parts Taraxacum mongolicum, 14 parts Dioscorea bulbifera, 12 parts Dioscorea bulbifera, 2 parts processed Aconitum carmichaelii, 10 parts Commelina communis, 10 parts Akebia trifoliata, 10 parts Alisma plantago-aquatica, 10 parts Ophiopogon japonicus, and 10 parts Adenophora stricta.

[0034] Example 3

[0035] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 26 parts Magnolia officinalis, 24 parts Taraxacum mongolicum, 28 parts Dioscorea bulbifera, 26 parts Dioscorea bulbifera, 8 parts processed Aconitum carmichaelii, 24 parts Commelina communis, 26 parts Akebia trifoliata, 24 parts Alisma plantago-aquatica, 26 parts Ophiopogon japonicus, and 24 parts Adenophora stricta.

[0036] Example 4

[0037] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 22 parts Magnolia officinalis, 24 parts Taraxacum mongolicum, 24 parts Dioscorea bulbifera, 26 parts Dioscorea bulbifera, 6 parts processed Aconitum carmichaelii, 22 parts Commelina communis, 22 parts Akebia trifoliata, 22 parts Alisma plantago-aquatica, 24 parts Ophiopogon japonicus, and 22 parts Adenophora stricta.

[0038] Example 5

[0039] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 12 parts Magnolia officinalis, 12 parts Taraxacum mongolicum, 12 parts Dioscorea bulbifera, 12 parts Dioscorea bulbifera, 4 parts processed Aconitum carmichaelii, 12 parts Commelina communis, 12 parts Akebia trifoliata, 12 parts Alisma plantago-aquatica, 12 parts Ophiopogon japonicus, and 12 parts Adenophora stricta.

[0040] Example 6

[0041] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 14 parts Magnolia officinalis, 12 parts Taraxacum mongolicum, 22 parts Dioscorea bulbifera, 20 parts Dioscorea bulbifera, 6 parts processed Aconitum carmichaelii, 18 parts Commelina communis, 14 parts Akebia trifoliata, 16 parts Alisma plantago-aquatica, 12 parts Ophiopogon japonicus, and 14 parts Adenophora stricta.

[0042] Example 7

[0043] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 16 parts Magnolia officinalis, 16 parts Taraxacum mongolicum, 24 parts Dioscorea bulbifera, 22 parts Dioscorea bulbifera, 3 parts processed Aconitum carmichaelii, 20 parts Commelina communis, 16 parts Akebia trifoliata, 14 parts Alisma plantago-aquatica, 14 parts Ophiopogon japonicus, and 16 parts Adenophora stricta.

[0044] Example 8

[0045] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 15 parts Magnolia officinalis, 17 parts Taraxacum mongolicum, 23 parts Dioscorea bulbifera, 21 parts Dioscorea bulbifera, 4 parts processed Aconitum carmichaelii, 21 parts Commelina communis, 15 parts Akebia trifoliata, 15 parts Alisma plantago-aquatica, 15 parts Ophiopogon japonicus, and 15 parts Adenophora stricta.

[0046] Example 9

[0047] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 18 parts Magnolia officinalis, 16 parts Taraxacum mongolicum, 25 parts Dioscorea bulbifera, 23 parts Dioscorea bulbifera, 3 parts processed Aconitum carmichaelii, 22 parts Commelina communis, 16 parts Akebia trifoliata, 13 parts Alisma plantago-aquatica, 17 parts Ophiopogon japonicus, and 18 parts Adenophora stricta.

[0048] Example 10

[0049] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 10 parts Magnolia officinalis, 10 parts Taraxacum mongolicum, 15 parts Dioscorea bulbifera, 15 parts Dioscorea bulbifera, 1 part processed Aconitum carmichaelii, 15 parts Commelina communis, 10 parts Akebia trifoliata, 10 parts Alisma plantago-aquatica, 10 parts Ophiopogon japonicus, and 10 parts Adenophora stricta.

[0050] Example 11

[0051] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 20 parts Magnolia officinalis, 20 parts Taraxacum mongolicum, 25 parts Dioscorea bulbifera, 25 parts Dioscorea bulbifera, 8 parts processed Aconitum carmichaelii, 25 parts Commelina communis, 20 parts Akebia trifoliata, 20 parts Alisma plantago-aquatica, 20 parts Ophiopogon japonicus, and 20 parts Adenophora stricta.

[0052] Experimental Example 1

[0053] A chicken farmer in Zhoukou City reported that 1,750 of his laying hens fell ill. The flock exhibited nasal discharge, and treatment with tylosin in the drinking water for three days did not alleviate the symptoms. Affected chickens became lethargic, their combs turned purple, and their feces became yellowish-green. Egg production dropped below 70%. Necropsy revealed congestion of the larynx and tracheal mucosa, no papillary hemorrhage in the proventriculus, petechiae in the cecal tonsils, congestion of follicles, and edema of the oviduct contents. Laboratory testing diagnosed the disease as a mild form of avian influenza, specifically H9 subtype.

[0054] Experimental drug:

[0055] Drug 1: Magnolia officinalis 16 parts, dandelion 14 parts, Dioscorea bulbifera 20 parts, Dioscorea bulbifera 18 parts, processed Aconitum carmichaelii 6 parts, Commelina communis 20 parts, Akebia trifoliata 16 parts, Alisma plantago-aquatica 14 parts, Ophiopogon japonicus 14 parts, Adenophora stricta 14 parts.

[0056] Drug 2: Magnolia officinalis 16 parts, dandelion 14 parts, prepared aconite 6 parts, Commelina communis 20 parts, Akebia trifoliata 16 parts, Alisma plantago-aquatica 14 parts, Ophiopogon japonicus 14 parts, Adenophora stricta 14 parts.

[0057] Drug 3: Magnolia officinalis 16 parts, Dioscorea bulbifera 20 parts, Dioscorea bulbifera 18 parts, processed Aconitum carmichaelii 6 parts, Akebia trifoliata 16 parts, Alisma plantago-aquatica 14 parts, Ophiopogon japonicus 14 parts, Adenophora stricta 14 parts.

[0058] Drug 4: Magnolia officinalis 16 parts, dandelion 14 parts, Dioscorea bulbifera 20 parts, Dioscorea bulbifera 18 parts, Commelina communis 20 parts, Ophiopogon japonicus 14 parts, Adenophora stricta 14 parts.

[0059] Drug 5: Dandelion 14 parts, Dioscorea bulbifera 20 parts, Dioscorea opposita 18 parts, processed Aconitum carmichaelii 6 parts, Commelina communis 20 parts, Akebia trifoliata 16 parts, Alisma plantago-aquatica 14 parts.

[0060] Drug 6: Jingfang Baidu San, prepared according to the traditional formula.

[0061] Weigh each raw material according to the weight proportions of each of the above formulas, wash and dry them, and then perform ultra-fine pulverization to obtain the traditional Chinese medicine powders of drugs 1-6.

[0062] The affected chickens were randomly divided into 7 groups of 250 each using a numerical randomization method: a control group and observation groups 1-6. The control group received no medication, while observation groups 1-6 were fed medication 1-6 respectively, at a dosage of 3 kg of medication mixed with 300 kg of feed. All groups had free access to feed and food, and all other feeding conditions remained the same. Each group was given medication for 5 consecutive days, and then observed for another 5 days after medication was stopped, with daily observation of changes in the flock and their health status.

[0063] Criteria for evaluating therapeutic efficacy:

[0064] Cured: Body temperature, feed intake, mental state, external symptoms such as comb return to normal, and egg production recovers to over 90%;

[0065] Effective: Body temperature is normal, feed intake increases, spirit is basically restored, external symptoms such as comb are significantly improved, and egg production recovers to more than 80%;

[0066] Ineffective: persistently high body temperature, decreased feed intake, lethargy, no improvement in external symptoms such as comb, and egg production continues to decline to below 70%.

[0067] The treatment effects on the sick chickens in each group are shown in Table 1.

[0068] Table 1 Treatment effects on sick chickens in each group

[0069] Grouping Quantity (pieces) Cured (only) Valid (only) Invalid (only) Death (only) cure rate Overall efficiency mortality rate Blank group 250 — — — 209 — — 83.6% Observation Group 1 250 211 36 3 0 84.4% 98.8% 0% Observation Group 2 250 143 96 11 3 57.2% 95.6% 1.2% Observation Group 3 250 157 84 9 1 62.8% 96.4% 0.4% Observation Group 4 250 170 72 8 1 68.0% 96.8% 0.4% Observation Group 5 250 161 81 8 1 64.4% 96.8% 0.4% Observation Group 6 250 145 77 28 6 58.0% 88.8% 2.4%

[0070] As shown in Table 1, the mortality rate of chickens in the control group without any drug treatment was as high as 83.6%. Compared with the control group, the total effective rate of treatment in observation groups 1-6 after different drug treatments was above 88%, indicating that drugs 1-6 had significant therapeutic effects on avian influenza and could significantly reduce the mortality rate of sick chickens. No deaths occurred in observation group 1 during the experiment. Mortality stopped in observation groups 2 and 3 on the 4th day of drug administration, in observation groups 4 and 5 on the 2nd day of drug administration, and in observation group 6 on the 5th day of drug administration; the mortality rate in observation group 1 was 0%. Although the total effective rate of treatment for avian influenza in chickens in all observation groups was above 90%, the cure rate of observation group 1 was 84.4%, while the cure rates of the other groups were between 55-70%. The cure rate of observation group 1 was significantly higher than that of the other observation groups. The above results indicate that the herbal composition of this invention has a more significant therapeutic effect on avian influenza than drugs 2-5 and Jingfang Baidu San, with a higher cure rate, significantly reducing the symptoms of sick laying hens, improving their mental state, increasing feed intake, increasing egg production, and reducing mortality. The herbal composition of this invention has a synergistic effect among the drugs, and each component is indispensable.

[0071] Experimental Example 2

[0072] 1 Experimental Drugs

[0073] (1) The original Chinese medicine liquid of the present invention: Weigh each raw material according to the following weight parts: 16 parts of Magnolia officinalis, 14 parts of Taraxacum mongolicum, 20 parts of Dioscorea bulbifera, 18 parts of Dioscorea bulbifera, 6 parts of processed Aconitum carmichaelii, 20 parts of Commelina communis, 16 parts of Akebia trifoliata, 14 parts of Alisma plantago-aquatica, 14 parts of Ophiopogon japonicus, and 14 parts of Adenophora stricta. Wash, dry, grind into powder, add 10 times (m / V) of distilled water, soak for 30 min, decoct for 1 h, obtain filtrate, add 8 times the amount of distilled water again and decoct for 1 h, combine the filtrates, concentrate to the raw drug content of 1 g / mL, and store at 4℃.

[0074] (2) Isatis root granules

[0075] (3) Astragalus polysaccharide oral liquid

[0076] 2. Experimental animals

[0077] A broiler farm in Zhoukou City discovered a sudden and severe outbreak of infectious bursal disease in a flock of healthy chickens. Symptoms included lethargy, head retraction, closed eyes, drowsiness, decreased appetite, ruffled feathers, increased water intake, and rice-water-like feces. Necropsy revealed brush-like hemorrhages on the affected areas and legs; the bursa of Fabricius was swollen or not, congested, and some chickens exhibited severe hemorrhage, appearing as purple grapes. Most of the bursa was yellow, with some containing yellowish-white gelatinous fluid. The duodenum showed hemorrhagic spots, and some had petechiae at the junction of the gizzard and proventriculus. The kidneys were enlarged or showed urate deposits. A small number of chickens died. No medication had been administered. Clinical observation and laboratory diagnosis confirmed infectious bursal disease in chickens, and the chickens were designated as experimental animals. Six hundred chickens were randomly selected from the affected flock and divided into four groups of 150 chickens each (30 days old). All experimental chickens were raised in standard chicken houses, using wire mesh floor rearing, and were immunized according to the routine immunization program, with free access to feed and water.

[0078] 3. Experimental Grouping and Treatment

[0079] The experimental animals were randomly divided into four groups of 150 chickens each: a blank control group, an observation group, control group 1, and control group 2. The blank control group received no medication and was fed normally. The observation group received the original herbal extract of this invention via drinking water at a concentration of 1% (1 mL of the original herbal extract diluted in 100 mL of water). Control group 1 received 3 g / L of Banlangen granules via drinking water. Control group 2 received 1% Astragalus polysaccharide oral solution via drinking water (1 mL of Astragalus polysaccharide oral solution diluted in 100 mL of water). The experiment lasted 14 days, including 7 days of treatment and 7 days of observation after treatment.

[0080] 3. Results Evaluation Indicators

[0081] 3.1 Observation of therapeutic effect

[0082] The effects of the traditional Chinese medicine composition of this invention on the cure rate, effectiveness, and mortality rate of chickens with infectious bursal disease were observed to determine the therapeutic effect.

[0083] (1) Cured: Clinical symptoms disappear, and mental state, appetite, feces, breathing, etc. return to normal;

[0084] (2) Effective: Chickens whose clinical symptoms have been alleviated are considered to be effective;

[0085] (3) Ineffective: The clinical symptoms of chickens did not improve or even worsened. The experimental chickens showed typical symptoms of infectious bursal disease and died. The death was determined to be caused by infectious bursal virus infection.

[0086] 3.2 Immune organ index

[0087] Seven days after administration, 10 chickens were randomly selected from each group, and each chicken was weighed. The bursa of Fabricius, thymus, and spleen were collected, and the immune organ index was calculated. Immune organ index = immune organ weight (mg) / body weight (g)

[0088] 4. Data Statistics and Analysis

[0089] Statistical analysis was performed using SPSS 21.0 software. Results are expressed as mean ± standard deviation (Mean ± SD). Graphpad Prism 8.0 software was used for statistical analysis and graphing. One-way ANOVA was used to compare differences between groups; p < 0.05 was considered statistically significant.

[0090] 5 Results

[0091] 5.1 Clinical efficacy

[0092] As shown in Table 2, the traditional Chinese medicine composition of the present invention can significantly reduce the mortality rate of infectious bursal disease in chickens and prevent death. The total effective rate of treatment for this disease can reach 98.67%, and the cure rate can reach 84.67%. The therapeutic effect is significantly better than that of Banlangen granules and Astragalus polysaccharide.

[0093] Table 2 Clinical efficacy results of each group

[0094] Group quantity Cured (only) Valid (only) Invalid (only) Death (only) cure rate Overall efficiency mortality rate Blank control group 150 — — 113 — — 75.33% Observation group 150 127 21 2 0 84.67% 98.67% 0.00% Control group 1 150 106 28 16 5 70.67% 89.33% 3.33% Control group 2 150 92 34 24 8 61.33% 84.00% 5.33%

[0095] 5.2 Immune Organ Index

[0096] Immune organ indices of each group of experimental chickens are as follows: Figure 1-3 As shown. Figure 1 The bursal index of each experimental group of chickens was calculated. Compared with the blank control group, the bursal index of the observation group and control groups 1-2 was significantly higher than that of the blank control group (P<0.05, P<0.01). Figure 2 The thymus index of chickens in each group of experiments was calculated. The thymus index of the observation group was significantly higher than that of the blank control group, with an extremely significant difference (P<0.01). The thymus index of control group 1 was slightly higher than that of the blank control group, with no significant difference (P>0.05). The thymus index of control group 2 was significantly higher than that of the blank control group, with a significant difference (P<0.05). Figure 3 The spleen index of each group of experimental chickens was calculated. The spleen index of the observation group was significantly higher than that of the blank control group, with an extremely significant difference (P<0.01); the spleen index of control groups 1-2 was significantly higher than that of the blank control group, with a significant difference (P>0.05).

[0097] 6 Conclusions

[0098] The above experimental results show that, compared with Banlangen granules and Astragalus polysaccharide oral liquid, the traditional Chinese medicine composition of the present invention has a higher efficacy and cure rate in the treatment of infectious bursal disease in chickens, can significantly reduce mortality, and has a significant therapeutic effect; at the same time, the traditional Chinese medicine composition of the present invention can significantly improve the immune organ index of diseased chickens, enhance the body's immunity, and improve the body's disease resistance.

[0099] Experimental Example 3

[0100] Experimental animals: Weaned piglets in a large-scale pig farm in Xinxiang City exhibited clinical symptoms such as anorexia, difficulty breathing, coughing, and lethargy. Serum antibody tests showed positive results for porcine circovirus, confirming the diagnosis of porcine circovirus disease. 160 affected piglets were selected from the affected pigpens and randomly divided into four groups of 40 piglets each: experimental groups 1-3 and a control group.

[0101] Experimental drugs and methods: Experimental groups 1-4 were fed the traditional Chinese medicine powders prepared in Examples 6-8, respectively. The raw materials were weighed according to the weight proportions of each formula described in Examples 6-8, washed, dried, and ultra-finely pulverized to obtain the traditional Chinese medicine powders of Examples 6-8. All groups were fed by mixing the powder with feed, with a dosage of 1 kg of powder mixed with 500 kg of piglet feed. The control group was administered Astragalus polysaccharide oral solution by gavage at a dosage of 0.5 mL / kg. The experiment lasted 14 days, with administration for 7 days and observation for 7 days after discontinuation of administration. Pigs had free access to feed and water during the experiment, and the pens were kept clean and hygienic, with regular disinfection. Clinical changes and cure rates of the experimental pigs were observed and recorded.

[0102] Efficacy evaluation criteria:

[0103] Cured: The patient is mentally normal, breathes normally, cough has disappeared, and clinical symptoms such as increased water and food intake have returned to normal have disappeared, and serum antibody tests are negative;

[0104] Effective: Normal breathing, restored mental state, increased water and food intake, significantly reduced cough, and other clinical symptoms improved; serum antibody test was negative.

[0105] Ineffective: Clinical symptoms do not improve or even worsen, death occurs, and serum antibody tests remain positive.

[0106] As shown in Table 3, the traditional Chinese medicine powders prepared in Examples 6-8, compared with the yellow polysaccharide oral liquid, all had a total effective rate of over 97% in treating porcine circovirus disease in piglets, and could achieve a total effective rate of 100%, thus avoiding mortality.

[0107] Table 3. Treatment efficacy against porcine circovirus.

[0108] Grouping quantity Heal (head) Valid (head) Invalid (header) Death (head) Overall efficiency Experimental group 1 40 31 9 0 0 100% Experimental group 2 40 29 10 1 0 97.5% Experimental group 3 40 33 7 0 0 100% control group 40 18 11 11 6 72.5%

[0109] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A traditional Chinese medicine composition for antiviral treatment of livestock and poultry, characterized in that, The traditional Chinese medicine composition is made from the following raw materials in parts by weight: 10-30 parts Magnolia officinalis, 10-30 parts Taraxacum mongolicum, 10-30 parts Dioscorea bulbifera, 10-30 parts Dioscorea bulbifera, 1-10 parts processed Aconitum carmichaelii, 10-30 parts Commelina communis, 10-30 parts Akebia trifoliata, 10-30 parts Alisma plantago-aquatica, 10-30 parts Ophiopogon japonicus, and 10-30 parts Adenophora stricta.

2. The traditional Chinese medicine composition for antiviral treatment of livestock and poultry according to claim 1, characterized in that, The traditional Chinese medicine composition is made from the following raw materials in parts by weight: 10-20 parts Magnolia officinalis, 10-20 parts Taraxacum mongolicum, 15-25 parts Dioscorea bulbifera, 15-25 parts Dioscorea bulbifera, 1-10 parts processed Aconitum carmichaelii, 15-25 parts Commelina communis, 10-20 parts Akebia trifoliata, 10-20 parts Alisma plantago-aquatica, 10-20 parts Ophiopogon japonicus, and 10-20 parts Adenophora stricta.

3. The traditional Chinese medicine composition for antiviral treatment of livestock and poultry according to claim 1, characterized in that, The traditional Chinese medicine composition is made from the following raw materials in parts by weight: 16 parts Magnolia officinalis, 14 parts Taraxacum mongolicum, 20 parts Dioscorea bulbifera, 18 parts Dioscorea bulbifera, 6 parts processed Aconitum carmichaelii, 20 parts Commelina communis, 16 parts Akebia trifoliata, 14 parts Alisma plantago-aquatica, 14 parts Ophiopogon japonicus, and 14 parts Adenophora stricta.

4. The application of the traditional Chinese medicine composition according to any one of claims 1 to 3 in the preparation of a drug for preventing and treating viral diseases in livestock and poultry, characterized in that, The traditional Chinese medicine composition is formulated into oral preparations that are accepted by animals, including granules, powders, pills, freeze-dried powders, and oral liquids.

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