A traditional Chinese medicine composition for treating chicken bronchial obstruction and a preparation method and application thereof

Abstract

The present application relates to a kind of traditional Chinese medicine composition for treating chicken bronchial obstruction and its preparation method and application, the traditional Chinese medicine composition is prepared by isatis root, houttuynia cordata, platycladus branchlet, orange red, peppermint, stemona, arctium lappa, curcuma, liquorice, eucalyptus leaf, borneol, mirabilite, pinellia, reed stem, wax gourd seed.The traditional Chinese medicine composition has the effects of clearing heat and resolving toxicity, cooling blood and promoting lung, relieving cough and asthma.The traditional Chinese medicine composition of the present application can prevent drug residues in vivo, has no toxic side effects, is safe and reliable, can be prepared into dosage forms such as powder or oral liquid, and is used for treating chicken bronchial obstruction, especially suitable for use in late stage of broiler breeding and egg laying period of laying hen.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese veterinary medicine technology, specifically relating to a traditional Chinese medicine composition for treating bronchial obstruction in chickens, its preparation method, and its application. Background Technology

[0002] Bronchial obstruction in chickens is a clinical symptom of certain diseases. Essentially, it is a respiratory disorder syndrome caused by a combination of pathogens, including infectious bronchitis virus, H9 subtype avian influenza virus, Mycoplasma gallisepticum, fungi, and Escherichia coli, resulting from poor husbandry conditions and weakened immunity. This syndrome is widespread in my country, with high morbidity and mortality rates, particularly in intensive poultry farms, and its prevalence is increasing year by year, causing significant economic losses to my country's poultry industry.

[0003] Bronchial obstruction in chickens is a complex disease caused by multiple factors, resulting in a clinical respiratory disorder syndrome. Clinical symptoms are primarily dyspnea and caseous bronchial blockage, often accompanied by air sacculitis, pericarditis, and decreased egg production. Symptomatic treatment is ineffective, and the infection rate is high; often, after a few cases in a flock, the entire flock can be affected within two or three days. Affected chickens experience decreased productivity, and in severe cases, the disease can cause widespread mortality in chicks.

[0004] Currently, there is no specific treatment for bronchial obstruction in chickens. Extensive use of antibiotics can only suppress secondary bacterial infections, treating the symptoms but not the root cause, and also poses serious public health risks. Research indicates that some traditional Chinese medicines with properties of clearing heat and detoxifying, cooling the blood, relieving sore throat, and eliminating phlegm and cough can significantly alleviate symptoms of bronchial obstruction.

[0005] Traditional Chinese medicine (TCM) is an important component of my country's traditional veterinary medicine and one of the treasures of Chinese culture. In recent years, with changes in the spectrum of livestock and poultry diseases and the rise of the "return to nature" trend, TCM has become a source of innovation in veterinary medicine, and its development and achievements have increasingly attracted worldwide attention. my country boasts a rich variety of TCM species, a large planting area, and a complete range of varieties. TCM has low toxicity and side effects, is less likely to induce drug resistance, and has advantages such as promoting growth, enhancing immune function, and improving disease resistance. Its application will open up a new avenue for the drug treatment of bronchial obstruction in chickens.

[0006] Based on the above, it is of great significance to develop a traditional Chinese medicine composition that can effectively treat bronchial obstruction in chickens, making it non-toxic, harmless, without side effects, and environmentally friendly. Summary of the Invention

[0007] Through in-depth and meticulous experimentation, this invention has successfully developed a traditional Chinese medicine composition for treating bronchial obstruction in chickens, and its efficacy has been experimentally proven. This traditional Chinese medicine composition possesses the effects of clearing heat and detoxifying, cooling the blood and promoting lung function, and relieving cough and asthma. Compared to antibiotics, hormones, and chemically synthesized drugs, it can prevent drug residues in the body, has no toxic side effects, and is safe and reliable. It is suitable for treating bronchial obstruction in chickens, especially for use in the later stages of broiler farming and during the egg-laying period of laying hens.

[0008] The specific technical solution of this invention is as follows:

[0009] In a first aspect, the present invention provides a traditional Chinese medicine composition for treating bronchial obstruction in chickens, the traditional Chinese medicine composition being prepared from Isatis root, Houttuynia cordata, Lepidium seed, Citrus reticulata peel, Mentha, Stemona japonica, Arctium lappa, Curcuma longa, Glycyrrhiza uralensis, Eucalyptus leaf, Borneol, Glauber's salt, Pinellia ternata, Reed stem, and Benincasa hispida seed.

[0010] The traditional Chinese medicine composition is prepared from the following raw materials in the following weight proportions: 10-150 parts of Isatis root, 10-60 parts of Houttuynia cordata, 10-55 parts of Lepidium apetalum, 10-50 parts of Citrus reticulata peel, 5-30 parts of Mentha haplocalyx, 10-40 parts of Stemona japonica, 10-50 parts of Arctium lappa, 3-25 parts of Curcuma longa, 5-50 parts of Glycyrrhiza uralensis, 60-150 parts of Eucalyptus globulus, 1-10 parts of Borneol, 1-20 parts of Glauber's salt, 5-20 parts of Pinellia ternata, 100-300 parts of Phragmites communis stem, and 100-200 parts of Benincasa hispida seeds.

[0011] Preferably, the traditional Chinese medicine composition is prepared from the following raw materials in the following weight proportions: 80-120 parts of Isatis root, 30-50 parts of Houttuynia cordata, 30-45 parts of Lepidium apetalum, 20-40 parts of Citrus reticulata peel, 10-15 parts of Mentha haplocalyx, 10-20 parts of Stemona japonica, 10-30 parts of Arctium lappa, 12-18 parts of Curcuma longa, 20-30 parts of Glycyrrhiza uralensis, 100-150 parts of Eucalyptus globulus, 3-6 parts of Borneol, 3-10 parts of Glauber's salt, 5-10 parts of Pinellia ternata, 150-200 parts of Phragmites communis stem, and 100-150 parts of Benincasa hispida seeds.

[0012] Preferably, the traditional Chinese medicine composition is prepared from the following raw materials in the following weight proportions: 100 parts of Isatis root, 40 parts of Houttuynia cordata, 40 parts of Lepidium seed, 33 parts of Citrus reticulata peel, 12 parts of Mentha haplocalyx, 20 parts of Stemona japonica, 30 parts of Arctium lappa, 14 parts of Curcuma longa, 28 parts of Glycyrrhiza uralensis, 120 parts of Eucalyptus leaf, 5 parts of Borneol, 5 parts of Glauber's salt, 8 parts of Pinellia ternata, 180 parts of Phragmites communis stem, and 100 parts of Benincasa hispida seed.

[0013] In a second aspect, the present invention provides a method for preparing a traditional Chinese medicine composition for treating bronchial obstruction in chickens. The traditional Chinese medicine composition is weighed according to the specified weight parts, pulverized and sieved separately, and then mixed evenly to prepare a traditional Chinese medicine powder for animal use.

[0014] In a third aspect, this invention provides another method for preparing a traditional Chinese medicine composition for treating bronchial obstruction in chickens, the preparation steps of which are as follows:

[0015] (1) Take four medicinal materials: houttuynia cordata, mint, eucalyptus leaves and winter melon seeds, place them in an extraction tank, add water equivalent to 15 to 30 times the weight of the medicinal materials, soak for 0.5 to 2 hours, heat to boiling, keep boiling for 6 to 12 hours, collect the volatile oil to obtain volatile oil A; filter the extract to obtain extract B;

[0016] (2) Take five herbs: Stemona japonica, Glycyrrhiza uralensis, Glauber's salt, Pinellia ternata, and Phragmites communis. Place them in an extraction tank and add water equivalent to 6 to 10 times the weight of the herbs. Reflux and extract 1 to 3 times, 1 to 3 hours each time. Combine the extracts and concentrate them together with extract B under reduced pressure to a clear paste with a relative density of 1.10 to 1.15 at 55°C. Cool the paste and add an appropriate amount of ethanol to make the alcohol content of the solution reach 40 to 75%. Let it stand, filter, and obtain the supernatant C.

[0017] (3) Take five herbs: Isatis root, Lepidium seed, Citrus reticulata peel, Arctium lappa seed, and Curcuma longa. Place them in an extraction tank and add ethanol of 6 to 10 times the weight of the herbs and a volume fraction of 40% to 75%. Reflux and extract 1 to 3 times, 0.5 to 2 hours each time. Combine the extracts and remove the ethanol from the supernatant C under reduced pressure until there is no alcohol taste. Continue to concentrate under reduced pressure to a thick paste with a relative density of 1.21 to 1.25 at 55°C. Dry at 40 to 100°C and pulverize to obtain the support raw material D.

[0018] In a fourth aspect, the present invention provides the application of a traditional Chinese medicine composition for treating bronchial obstruction in chickens in the preparation of a traditional Chinese medicine preparation for treating bronchial obstruction in chickens.

[0019] Preferably, the traditional Chinese medicine preparation is a traditional Chinese medicine powder or a traditional Chinese medicine oral liquid.

[0020] The preparation process of the traditional Chinese medicine powder is as follows:

[0021] (1) Weigh each raw material according to the above weight proportions, and prepare volatile oil A and stabilizing raw material D according to the preparation method of the traditional Chinese medicine composition;

[0022] (2) Take 5 to 10 times the mass of volatile oil A of β-cyclodextrin, add 15 to 30 times the mass of cyclodextrin of purified water, and stir evenly at 25 to 50°C.

[0023] (3) Take volatile oil A, dissolve it in ethanol with 1 to 3 times the mass of volatile oil A, and slowly drop it into the cyclodextrin solution under stirring. After the drop is finished, stir for another 30 minutes, filter, dry the precipitate at low temperature until dry, and pulverize it to obtain inclusion complex E.

[0024] (4) Take borneol, crush it, and mix it evenly with the plugging raw material D and the inclusion compound E to make a plugging powder.

[0025] The preparation process of the oral liquid of traditional Chinese medicine is as follows:

[0026] (1) Weigh each raw material according to the above weight proportions, and prepare volatile oil A and stabilizing raw material D according to the preparation method of the traditional Chinese medicine composition;

[0027] (2) Take volatile oil A and borneol, add 5-30% of the total volume of ethanol, stir to dissolve, and obtain ethanol solution F;

[0028] (3) Take the raw material D for bronchodilator, place it in a mixing tank, add 50% of the total volume of purified water, stir to dissolve, add ethanol solution F, stir evenly, refrigerate for 24 hours, filter, add purified water to make up the volume, mix evenly, and make bronchodilator oral solution.

[0029] The traditional Chinese medicine preparation has the effects of clearing heat and detoxifying, cooling blood and ventilating the lungs, relieving cough and asthma, and is used to treat bronchial obstruction in chickens. It is especially suitable for use in the later stage of broiler breeding and during the egg-laying period of laying hens.

[0030] The traditional Chinese medicine composition of this invention is prepared from natural plants, including: Isatis root (Banlangen) for clearing heat and detoxifying, cooling blood and relieving sore throat; Houttuynia cordata for clearing heat and detoxifying, reducing swelling and draining pus; Lepidium seed for purging lung heat and relieving asthma; Citrus reticulata peel for drying dampness and resolving phlegm, regulating qi and relieving chest congestion; Mentha haplocalyx for dispelling wind and heat, and relieving sore throat; Stemona japonica for moistening the lungs and relieving cough; Arctium lappa for dispelling wind-heat, clearing the lungs, detoxifying and relieving sore throat; Curcuma longa for regulating qi and relieving depression, cooling blood and promoting blood circulation; Eucalyptus leaf for dispelling wind and relieving exterior symptoms, clearing heat and detoxifying; Borneol for opening the orifices and refreshing the mind, clearing heat and relieving pain; Glauber's salt for moistening dryness and softening hardness, clearing heat and reducing swelling; Pinellia ternata for warming the middle jiao and resolving phlegm; Phragmites communis stem for clearing lung heat and detoxifying, relieving cough and draining pus; Benincasa hispida seed for clearing lung heat and resolving phlegm, eliminating carbuncles and draining pus; and Glycyrrhiza uralensis for tonifying the spleen and replenishing qi, resolving phlegm and relieving cough, harmonizing the middle jiao and relieving spasms, detoxifying, and harmonizing the effects of the other herbs. The combined effects of these herbs are to clear heat and detoxify, cool blood and clear the lungs, and relieve cough and asthma.

[0031] The beneficial effects of this invention are:

[0032] 1. The traditional Chinese medicine composition of the present invention is prepared from natural Chinese herbal medicines and has the advantages of long duration of action, mild effect and little stimulation to animal body. Compared with antibiotics, hormones and chemically synthesized drugs, it can prevent drug residues in the body, has no toxic side effects and is safe and reliable.

[0033] 2. The traditional Chinese medicine composition of the present invention has significant effects, including clearing heat and detoxifying, cooling blood and ventilating the lungs, relieving cough and asthma. It is used to treat bronchial obstruction in chickens, and is especially suitable for use in the later stages of broiler breeding and during the egg-laying period of laying hens.

[0034] 3. The traditional Chinese medicine composition of the present invention improves the utilization rate of medicinal materials, greatly saves medicinal materials, and avoids waste.

[0035] 4. The traditional Chinese medicine composition of the present invention can be made into oral liquid or powder for use in drinking water or mixed with feed, according to the animal's feeding habits.

[0036] 5. The preparation process of this invention is simple, easy to use, the raw materials are readily available, the price is low, and it is green and environmentally friendly. Detailed Implementation

[0037] Example 1: Preparation of the plugging agent

[0038] Formula composition: 100kg Isatis root, 40kg Houttuynia cordata, 40kg Lepidium apetalum, 33kg Citrus reticulata peel, 12kg Mentha haplocalyx, 20kg Stemona japonica, 30kg Arctium lappa, 14kg Curcuma longa, 28kg Glycyrrhiza uralensis, 120kg Eucalyptus globulus, 5kg Borneol, 5kg Glauber's salt, 8kg Pinellia ternata, 180kg Phragmites communis stem, 100kg Benincasa hispida seeds.

[0039] Preparation method: Weigh the above-mentioned medicinal materials according to the prescription amount, crush them separately, pass them through a 24-mesh sieve, and mix them evenly to obtain Zhidu powder (each 1g is equivalent to 1g of raw medicinal material).

[0040] Example 2: Preparation of the plugging agent

[0041] Formula composition: 10kg Isatis root, 60kg Houttuynia cordata, 10kg Lepidium seed, 50kg Citrus reticulata peel, 5kg Mentha, 40kg Stemona japonica, 10kg Arctium lappa, 25kg Curcuma longa, 5kg Glycyrrhiza uralensis, 150kg Eucalyptus leaf, 1kg Borneol, 20kg Glauber's salt, 5kg Pinellia ternata, 300kg Reed stem, 100kg Winter melon seed.

[0042] Preparation method:

[0043] (1) Take four medicinal materials: houttuynia cordata, mint, eucalyptus leaves and winter melon seeds, put them in an extraction tank, add 4725L of water, soak for 1 hour, heat to boiling, keep boiling for 12 hours, collect the volatile oil, and obtain 17.1L of volatile oil (A); filter the extract to obtain extract B.

[0044] (2) Take five herbs: Stemona japonica, Glycyrrhiza uralensis, Sodium sulfate, Pinellia ternata, and Phragmites communis. Place them in an extraction tank, add 3700L of water, and reflux once for 1.5 hours each time. Combine the extracts and concentrate them together with extract B under reduced pressure to a clear paste with a relative density of 1.10 to 1.15 at 55°C. Cool the paste, add an appropriate amount of ethanol to make the alcohol content of the solution reach 75%, let it stand, filter, and obtain the supernatant C.

[0045] (3) Take five herbs: Isatis root, Lepidium seed, Citrus peel, Arctium seed, and Curcuma longa, place them in an extraction tank, add 1050L of 75% ethanol, reflux and extract once, each time for 1 hour, combine the extracts, and recover the ethanol under reduced pressure with the supernatant C until there is no alcohol taste, continue to concentrate under reduced pressure to a thick paste with a relative density of 1.21 to 1.25 at 55℃, dry at 100℃, pulverize, and obtain 56.2kg (D) of the raw material for plugging.

[0046] (4) Take 85.5 kg of β-cyclodextrin, add 1282.5 L of purified water, and stir evenly at 25°C;

[0047] (5) Take volatile oil A, dissolve it in 17.1L of ethanol, and slowly add it dropwise into the cyclodextrin solution under stirring. After the dropwise addition is complete, stir for another 30 minutes, filter, dry the precipitate at low temperature until dry, and pulverize it to obtain 78.7kg (E) of inclusion complex.

[0048] (6) Borneol is crushed and mixed evenly with the raw material D, inclusion complex E and 615.1 kg of anhydrous glucose to obtain the Zhidu powder (each 1g is equivalent to 1g of raw drug).

[0049] Example 3: Preparation of the plugging agent

[0050] Formula composition: 150kg Isatis root, 10kg Houttuynia cordata, 55kg Lepidium seed, 10kg Citrus reticulata peel, 30kg Mentha, 10kg Stemona japonica, 50kg Arctium lappa, 3kg Curcuma longa, 50kg Glycyrrhiza uralensis, 60kg Eucalyptus leaf, 10kg Borneol, 1kg Glauber's salt, 20kg Pinellia ternata, 100kg Reed stem, 200kg Winter melon seed.

[0051] Preparation method:

[0052] (1) Take four medicinal materials: houttuynia cordata, mint, eucalyptus leaves and winter melon seeds, put them in an extraction tank, add 6000L of water, soak for 1.5 hours, heat to boiling, keep boiling for 6 hours, collect the volatile oil, and obtain 29.6L of volatile oil (A); filter the extract to obtain extract B.

[0053] (2) Take five herbs: Stemona japonica, Glycyrrhiza uralensis, Sodium sulfate, Pinellia ternata, and Phragmites communis. Place them in an extraction tank, add 1629L of water, and reflux extract twice for 2 hours each time. Combine the extracts and concentrate them together with extract B under reduced pressure to a clear paste with a relative density of 1.10 to 1.15 at 55°C. Cool the paste, add an appropriate amount of ethanol to make the alcohol content of the solution reach 70%, let it stand, filter it, and obtain the supernatant C.

[0054] (3) Take five herbs: Isatis root, Lepidium seed, Citrus peel, Arctium seed, and Curcuma longa, place them in an extraction tank, add 2412L of 70% ethanol, reflux and extract twice, 1.5 hours each time, combine the extracts, and recover the ethanol under reduced pressure with the supernatant C until there is no alcohol taste, continue to concentrate under reduced pressure to a thick paste with a relative density of 1.21 to 1.25 at 55℃, dry at 90℃, pulverize, and obtain 86.9kg (D) of the raw material for plugging.

[0055] (4) Take 222 kg of β-cyclodextrin, add 4440 L of purified water, and stir evenly at 30 °C;

[0056] (5) Take volatile oil A, dissolve it in 59.2L of ethanol, and slowly add it dropwise to the cyclodextrin solution under stirring. After the addition is complete, stir for another 30 minutes, filter, dry the precipitate at low temperature until dry, and pulverize to obtain 188.7kg (E) of inclusion complex.

[0057] (6) Borneol is crushed and mixed evenly with the raw material D, inclusion complex E and 463.4 kg of anhydrous glucose to obtain Zhidu powder (each 1g is equivalent to 1g of raw drug).

[0058] Example 4: Preparation of the plugging agent

[0059] Formula composition: 120kg Isatis root, 30kg Houttuynia cordata, 45kg Lepidium seed, 20kg Citrus reticulata peel, 15kg Mentha, 10kg Stemona japonica, 30kg Arctium lappa, 12kg Curcuma longa, 30kg Glycyrrhiza uralensis, 100kg Eucalyptus leaf, 6kg Borneol, 3kg Glauber's salt, 10kg Pinellia ternata, 150kg Reed stem, 150kg Winter melon seed.

[0060] Preparation method:

[0061] (1) Take four medicinal materials: houttuynia cordata, mint, eucalyptus leaves and winter melon seeds, put them in an extraction tank, add 7375L of water, soak for 2 hours, heat to boiling, keep boiling for 8 hours, collect the volatile oil, and obtain 23.2L of volatile oil (A); filter the extract to obtain extract B.

[0062] (2) Take five herbs: Stemona japonica, Glycyrrhiza uralensis, Sodium sulfate, Pinellia ternata, and Phragmites communis. Place them in an extraction tank, add 1624L of water, and reflux extract three times for three hours each time. Combine the extracts and concentrate them together with extract B under reduced pressure to a clear paste with a relative density of 1.10 to 1.15 at 55°C. Cool the paste, add an appropriate amount of ethanol to make the alcohol content of the solution reach 65%, let it stand, filter it, and obtain the supernatant C.

[0063] (3) Take five herbs: Isatis root, Lepidium seed, Citrus reticulata peel, Arctium lappa seed, and Curcuma longa. Place them in an extraction tank and add 1816 L of 65% ethanol. Reflux and extract three times, each time for 2 hours. Combine the extracts and remove the ethanol from the supernatant C under reduced pressure until there is no alcohol taste. Continue to concentrate under reduced pressure to a thick paste with a relative density of 1.21 to 1.25 at 55°C. Dry at 80°C and pulverize to obtain 97.6 kg (D) of the raw material for plugging.

[0064] (4) Take 174 kg of β-cyclodextrin, add 4350 L of purified water, and stir evenly at 40 °C;

[0065] (5) Take volatile oil A, dissolve it in 69.6L of ethanol, and slowly add it dropwise to the cyclodextrin solution under stirring. After the addition is complete, stir for another 30 minutes, filter, dry the precipitate at low temperature until dry, and pulverize to obtain 151.8kg (E) of inclusion complex.

[0066] (6) Borneol is crushed and mixed evenly with the raw material D, inclusion complex E and 465.6 kg of anhydrous glucose to obtain Zhidu powder (each 1g is equivalent to 1g of raw drug).

[0067] Example 5: Preparation of the plugging agent

[0068] Formula composition: 80kg Isatis root, 50kg Houttuynia cordata, 30kg Lepidium seed, 40kg Citrus reticulata peel, 10kg Mentha, 20kg Stemona japonica, 10kg Arctium lappa, 18kg Curcuma longa, 20kg Glycyrrhiza uralensis, 150kg Eucalyptus leaf, 3kg Borneol, 10kg Glauber's salt, 5kg Pinellia ternata, 200kg Reed stem, 100kg Winter melon seed.

[0069] Preparation method:

[0070] (1) Take four medicinal materials: houttuynia cordata, mint, eucalyptus leaves and winter melon seeds, put them in an extraction tank, add 9300L of water, soak for 0.5 hours, heat to boiling, keep boiling for 10 hours, collect the volatile oil, and obtain 19.1L of volatile oil (A); filter the extract to obtain extract B.

[0071] (2) Take five herbs: Stemona japonica, Glycyrrhiza uralensis, Sodium sulfate, Pinellia ternata, and Phragmites communis. Place them in an extraction tank, add 1785L of water, and reflux extract twice for 1 hour each time. Combine the extracts and concentrate them together with extract B under reduced pressure to a clear paste with a relative density of 1.10 to 1.15 at 55°C. Cool the paste, add an appropriate amount of ethanol to make the alcohol content of the solution reach 60%, let it stand, filter, and obtain the supernatant C.

[0072] (3) Take five herbs: Isatis root, Lepidium seed, Citrus reticulata peel, Arctium lappa seed, and Curcuma longa. Place them in an extraction tank, add 1246L of 60% ethanol, and reflux extract twice for 0.5 hours each time. Combine the extracts and remove the ethanol from the supernatant C under reduced pressure until there is no alcohol taste. Continue to concentrate under reduced pressure to a thick paste with a relative density of 1.21 to 1.25 at 55°C. Dry at 70°C and pulverize to obtain 106.4kg (D) of the raw material for plugging.

[0073] (4) Take 191 kg of β-cyclodextrin, add 5730 L of purified water, and stir evenly at 50 °C.

[0074] (5) Take volatile oil A, dissolve it in 38.2L of ethanol, and slowly add it dropwise to the cyclodextrin solution under stirring. After the addition is complete, stir for another 30 minutes, filter, dry the precipitate at low temperature until dry, and pulverize to obtain 167.7kg (E) of inclusion complex.

[0075] (6) Borneol is crushed and mixed evenly with the raw material D, inclusion complex E and 448.9 kg of anhydrous glucose to obtain Zhidu powder (each 1g is equivalent to 1g of raw drug).

[0076] Example 6: Preparation of Zhidu Oral Solution

[0077] Formula composition: 100kg Isatis root, 40kg Houttuynia cordata, 40kg Lepidium apetalum, 33kg Citrus reticulata peel, 12kg Mentha haplocalyx, 20kg Stemona japonica, 30kg Arctium lappa, 14kg Curcuma longa, 28kg Glycyrrhiza uralensis, 120kg Eucalyptus globulus, 5kg Borneol, 5kg Glauber's salt, 8kg Pinellia ternata, 180kg Phragmites communis stem, 100kg Benincasa hispida seeds.

[0078] Preparation method:

[0079] (1) Take four medicinal materials: houttuynia cordata, mint, eucalyptus leaves and winter melon seeds, put them in an extraction tank, add 5440L of water, soak for 1 hour, heat to boiling, keep boiling for 8 hours, collect the volatile oil, and obtain 16.6L of volatile oil (A); filter the extract to obtain extract B.

[0080] (2) Take five herbs: Stemona japonica, Glycyrrhiza uralensis, Sodium sulfate, Pinellia ternata, and Phragmites communis. Place them in an extraction tank, add 1928L of water, and reflux extract twice for 1.5 hours each time. Combine the extracts and concentrate them together with extract B under reduced pressure to a clear paste with a relative density of 1.10 to 1.15 at 55°C. Cool the paste, add an appropriate amount of ethanol to make the alcohol content of the solution reach 60%, let it stand, filter it, and obtain the supernatant C.

[0081] (3) Take five herbs: Isatis root, Lepidium seed, Citrus reticulata peel, Arctium lappa seed, and Curcuma longa. Place them in an extraction tank, add 1736L of 60% ethanol, and reflux extract twice for 1 hour each time. Combine the extracts and remove the ethanol from the supernatant C under reduced pressure until there is no alcohol taste. Continue to concentrate under reduced pressure to a thick paste with a relative density of 1.21 to 1.25 at 55°C. Dry at 65°C and pulverize to obtain 113.4kg (D) of the raw material for plugging.

[0082] (4) Take volatile oil A and borneol, add 35.75L of ethanol, stir to dissolve, and obtain ethanol solution F;

[0083] (5) Take the raw material D of Zhidu, put it in a mixing tank, add 357.5L of purified water, stir to dissolve, add ethanol solution F, stir evenly, refrigerate for 24 hours, filter, add purified water to make up to 715L, mix evenly, and make Zhidu oral solution (each 1ml is equivalent to 1g of raw drug).

[0084] Example 7: Preparation of Zhidu Oral Solution

[0085] Formula composition: 10kg Isatis root, 10kg Houttuynia cordata, 10kg Lepidium seed, 10kg Citrus reticulata peel, 5kg Mentha, 10kg Stemona japonica, 10kg Arctium lappa, 3kg Curcuma longa, 30kg Glycyrrhiza uralensis, 150kg Eucalyptus leaves, 6kg Borneol, 10kg Glauber's salt, 10kg Pinellia ternata, 200kg Reed stem, 150kg Winter melon seeds.

[0086] Preparation method:

[0087] (1) Take four medicinal materials: houttuynia cordata, mint, eucalyptus leaves and winter melon seeds, put them in an extraction tank, add 9450L of water, soak for 1.5 hours, heat to boiling, keep boiling for 6 hours, collect the volatile oil, and obtain 24.8L of volatile oil (A); filter the extract to obtain extract B.

[0088] (2) Take five herbs: Stemona japonica, Glycyrrhiza uralensis, Sodium sulfate, Pinellia ternata, and Phragmites communis. Place them in an extraction tank, add 1560L of water, and reflux for extraction three times, each time for 2 hours. Combine the extracts and concentrate them together with extract B under reduced pressure to a clear paste with a relative density of 1.10 to 1.15 at 55°C. Cool the paste, add an appropriate amount of ethanol to make the alcohol content of the solution reach 55%, let it stand, filter it, and obtain the supernatant C.

[0089] (3) Take five herbs: Isatis root, Lepidium seed, Citrus peel, Arctium seed, and Curcuma longa, place them in an extraction tank, add 258L of 55% ethanol, reflux and extract 3 times, 1.5 hours each time, combine the extracts, and recover the ethanol under reduced pressure with the supernatant C until there is no alcohol taste, continue to concentrate under reduced pressure to a thick paste with a relative density of 1.21 to 1.25 at 55℃, dry at 60℃, pulverize, and obtain 77.1kg (D) of the raw material for plugging.

[0090] (4) Take volatile oil A and borneol, add 92.1L of ethanol, stir to dissolve, and obtain ethanol solution F;

[0091] (5) Take the raw material D of Zhidu, put it in a mixing tank, add 307L of purified water, stir to dissolve, add ethanol solution F, stir evenly, refrigerate for 24 hours, filter, add purified water to make up to 614L, mix evenly, and make Zhidu oral solution (each 1ml is equivalent to 1g of raw drug).

[0092] Example 8: Preparation of Zhidu Oral Solution

[0093] Formula composition: 150kg Isatis root, 60kg Houttuynia cordata, 55kg Lepidium seed, 50kg Citrus reticulata peel, 30kg Mentha, 40kg Stemona japonica, 50kg Arctium lappa, 25kg Curcuma longa, 20kg Glycyrrhiza uralensis, 100kg Eucalyptus leaf, 3kg Borneol, 3kg Glauber's salt, 5kg Pinellia ternata, 150kg Reed stem, 100kg Winter melon seed.

[0094] Preparation method:

[0095] (1) Take four medicinal materials: houttuynia cordata, mint, eucalyptus leaves and winter melon seeds, put them in an extraction tank, add 7250L of water, soak for 2 hours, heat to boiling, keep boiling for 8 hours, collect the volatile oil, and obtain 16.4L of volatile oil (A); filter the extract to obtain extract B.

[0096] (2) Take five herbs: Stemona japonica, Glycyrrhiza uralensis, Sodium sulfate, Pinellia ternata, and Phragmites communis. Place them in an extraction tank, add 1526L of water, and reflux once for 3 hours each time. Combine the extracts and concentrate them together with extract B under reduced pressure to a clear paste with a relative density of 1.10 to 1.15 at 55°C. Cool the paste, add an appropriate amount of ethanol to make the alcohol content of the solution reach 50%, let it stand, filter it, and obtain the supernatant C.

[0097] (3) Take five herbs: Isatis root, Lepidium seed, Citrus reticulata peel, Arctium lappa seed, and Curcuma longa. Place them in an extraction tank, add 2310L of 50% ethanol, and reflux extract once for 2 hours each time. Combine the extracts and remove the ethanol from the supernatant C under reduced pressure until there is no alcohol taste. Continue to concentrate under reduced pressure to a thick paste with a relative density of 1.21 to 1.25 at 55°C. Dry at 55°C and pulverize to obtain 187.7kg (D) of the raw material for plugging.

[0098] (4) Take volatile oil A and borneol, add 200.2L of ethanol, stir to dissolve, and obtain ethanol solution F;

[0099] (5) Take the raw material D of Zhidu, put it in a mixing tank, add 400.5L of purified water, stir to dissolve, add ethanol solution F, stir evenly, refrigerate for 24 hours, filter, add purified water to make up to 801L, mix evenly, and make Zhidu oral solution (each 1ml is equivalent to 1g of raw drug).

[0100] Example 9: Preparation of Zhidu Oral Solution

[0101] Formula composition: 120kg Isatis root, 50kg Houttuynia cordata, 45kg Lepidium seed, 40kg Citrus reticulata peel, 15kg Mentha, 20kg Stemona japonica, 30kg Arctium lappa, 18kg Curcuma longa, 5kg Glycyrrhiza uralensis, 60kg Eucalyptus leaves, 1kg Borneol, 1kg Glauber's salt, 5kg Pinellia ternata, 100kg Reed stem, 100kg Winter melon seeds.

[0102] Preparation method:

[0103] (1) Take four medicinal materials: houttuynia cordata, mint, eucalyptus leaves and winter melon seeds, put them in an extraction tank, add 4500L of water, soak for 0.5 hours, heat to boiling, keep boiling for 10 hours, collect the volatile oil, and obtain 15.4L of volatile oil (A); filter the extract to obtain extract B.

[0104] (2) Take five herbs: Stemona japonica, Glycyrrhiza uralensis, Sodium sulfate, Pinellia ternata, and Phragmites communis. Place them in an extraction tank, add 1179L of water, and reflux for extraction three times, one hour each time. Combine the extracts and concentrate them together with extract B under reduced pressure to a clear paste with a relative density of 1.10 to 1.15 at 55°C. Cool the paste, add an appropriate amount of ethanol to make the alcohol content of the solution reach 45%, let it stand, filter, and obtain the supernatant C.

[0105] (3) Take five herbs: Isatis root, Lepidium seed, Citrus reticulata peel, Arctium lappa seed, and Curcuma longa. Place them in an extraction tank, add 2277L of 45% ethanol, and reflux extract three times for 0.5 hours each time. Combine the extracts and remove the ethanol from the supernatant C under reduced pressure until there is no alcohol taste. Continue to concentrate under reduced pressure to a thick paste with a relative density of 1.21 to 1.25 at 55°C. Dry at 50°C and pulverize to obtain 156.9kg (D) of the raw material for plugging.

[0106] (4) Take volatile oil A and borneol, add 177L of ethanol, stir to dissolve, and obtain ethanol solution F;

[0107] (5) Take the raw material D of the bronchodilator, put it in a mixing tank, add 295L of purified water, stir to dissolve, add ethanol solution F, stir evenly, refrigerate for 24 hours, filter, add purified water to make up to 590L, mix evenly, and make the bronchodilator oral solution (each 1ml is equivalent to 1g of raw drug).

[0108] Example 10: Preparation of Zhidu Oral Solution

[0109] Formula composition: 80kg Isatis root, 30kg Houttuynia cordata, 30kg Lepidium seed, 20kg Citrus reticulata peel, 10kg Mentha, 10kg Stemona japonica, 10kg Arctium lappa, 12kg Curcuma longa, 50kg Glycyrrhiza uralensis, 150kg Eucalyptus leaves, 10kg Borneol, 20kg Glauber's salt, 20kg Pinellia ternata, 300kg Phragmites communis stem, 200kg Benincasa hispida seeds.

[0110] Preparation method:

[0111] (1) Take four medicinal materials: houttuynia cordata, mint, eucalyptus leaves and winter melon seeds, put them in an extraction tank, add 5850L of water, soak for 1 hour, heat to boiling, keep boiling for 12 hours, collect the volatile oil, and obtain 31.1L of volatile oil (A); filter the extract to obtain extract B.

[0112] (2) Take five herbs: Stemona japonica, Glycyrrhiza uralensis, Sodium sulfate, Pinellia ternata, and Phragmites communis. Place them in an extraction tank, add 4000L of water, and reflux once for 1.5 hours each time. Combine the extracts and concentrate them together with extract B under reduced pressure to a clear paste with a relative density of 1.10 to 1.15 at 55°C. Cool the paste, add an appropriate amount of ethanol to make the alcohol content of the solution reach 40%, let it stand, filter it, and obtain the supernatant C.

[0113] (3) Take five herbs: Isatis root, Lepidium seed, Citrus reticulata peel, Arctium lappa seed, and Curcuma longa. Place them in an extraction tank, add 1520L of 40% ethanol, and reflux extract once for 1 hour each time. Combine the extracts and remove the ethanol from the supernatant C under reduced pressure until there is no alcohol taste. Continue to concentrate under reduced pressure to a thick paste with a relative density of 1.21 to 1.25 at 55°C. Dry at 40°C and pulverize to obtain 193kg (D) of the raw material for the plugging.

[0114] (4) Take volatile oil A and borneol, add 188.4L of ethanol, stir to dissolve, and obtain ethanol solution F;

[0115] (5) Take the raw material D of the bronchodilator, put it in a mixing tank, add 471L of purified water, stir to dissolve, add ethanol solution F, stir evenly, refrigerate for 24 hours, filter, add purified water to make up to 942L, mix evenly, and make the bronchodilator oral solution (each 1ml is equivalent to 1g of raw drug).

[0116] Comparative Example 1: Conventional Extraction and Preparation Process

[0117] Formula composition: 100kg Isatis root, 40kg Houttuynia cordata, 40kg Lepidium apetalum, 33kg Citrus reticulata peel, 12kg Mentha haplocalyx, 20kg Stemona japonica, 30kg Arctium lappa, 14kg Curcuma longa, 28kg Glycyrrhiza uralensis, 120kg Eucalyptus globulus, 5kg Borneol, 5kg Glauber's salt, 8kg Pinellia ternata, 180kg Phragmites communis stem, 100kg Benincasa hispida seeds.

[0118] Preparation method: Weigh the above-mentioned medicinal materials according to the prescription amount, place them in an extraction tank, add 5880L of water, reflux and extract twice, 1.5 hours each time, filter, combine the filtrates, concentrate the filtrate under reduced pressure to a clear extract with a relative density of 1.10-1.15 at 55℃, place it in a dissolving tank, add 2.2kg of potassium sorbate, stir for 10 minutes to dissolve, refrigerate for 24 hours, filter, add purified water to make up to 735L, mix well, and prepare a comparative oral solution for bronchodilator (each 1ml is equivalent to 1g of raw medicinal material).

[0119] Comparative Example 2 was prepared according to the formulation and method of Example 1 in patent CN112089799A.

[0120] Formula composition: 50kg reed stems, 75kg coix seed, 30kg almond, 80kg winter melon seeds, 30kg peach kernel, 60kg mulberry bark, 60kg houttuynia cordata, 75kg honeysuckle, 65kg coltsfoot flower, and 15kg licorice.

[0121] Preparation method: Place the above-mentioned medicinal materials, except for honeysuckle, in an extraction tank, add 2160L of water and soak for 12 hours, heat to boiling, decoct for 0.5 hours, add honeysuckle, continue to decoct for 1.5 hours, discharge, cool, and filter; add 1080L of water to the dregs, heat to boiling, decoct for 1.5 hours, discharge, cool, and filter; combine the filtrates, precipitate at room temperature for 8 hours, filter, and concentrate the filtrate under reduced pressure to a liquid with a relative density of not less than 1.15 at 55℃, about 270L, to obtain the Zhidu comparative oral liquid (each 1ml is equivalent to 2g of raw medicinal material).

[0122] Example 11 Clinical efficacy trial

[0123] Experimental Methods: 840 21-day-old commercial broilers were randomly divided into 14 groups, with 3 replicates per group and 20 birds per replicate. Except for the control group, all other groups were challenged with IBV via nasal or ocular drops. The IBV infection dose was 10... 6.62 EID 50 0.1ml, H9 infection dose is 10 8.5 EID 50 0.1ml. Seven days after infection, each group received the corresponding treatment for five consecutive days, as detailed in Table 1.

[0124] Table 1. Trial Groups and Drug Administration

[0125]

[0126]

[0127] The experimental results show that:

[0128] (1) The chickens in the model group showed reduced feed and water intake and exhibited respiratory symptoms, including stretching their necks and panting with their mouths open, retracting their heads and closing their eyes, drooping their wings and huddling together, lethargy, and weak squatting. Some chickens died. Necropsy revealed hemorrhages and mucus in the trachea. This indicates that the bronchial obstruction model was successfully established.

[0129] (2) After 5 days of continuous administration, respiratory symptoms in all experimental groups basically disappeared. Autopsy revealed that the tracheal hemorrhages and mucus in the experimental groups had disappeared, while the hemorrhages and mucus in the model group were still present. This indicates that the drugs in all experimental groups have a therapeutic effect on bronchial obstruction.

[0130] (3) All experimental groups showed the effect of restoring the weight, feed intake, and feed conversion ratio of chickens with bronchial obstruction. Among them, experimental groups 1, 4, and 6 showed the best results, especially experimental group 6. See Table 2 for details.

[0131] Table 2 Statistical analysis of experimental results

[0132]

[0133] While specific embodiments of the present invention have been described above, those skilled in the art should understand that the specific embodiments described are merely illustrative and not intended to limit the scope of the present invention. Equivalent modifications and variations made by those skilled in the art in accordance with the spirit of the present invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. A traditional Chinese medicine composition for treating bronchial obstruction in chickens, characterized in that, The traditional Chinese medicine composition is prepared from the following raw materials in the following weight proportions: 80-120 parts of Isatis root, 30-50 parts of Houttuynia cordata, 30-45 parts of Lepidium apetalum, 20-40 parts of Citrus reticulata peel, 10-15 parts of Mentha haplocalyx, 10-20 parts of Stemona japonica, 10-30 parts of Arctium lappa, 12-18 parts of Curcuma longa, 20-30 parts of Glycyrrhiza uralensis, 100-150 parts of Eucalyptus globulus, 3-6 parts of Borneol, 3-10 parts of Glauber's salt, 5-10 parts of Pinellia ternata, 150-200 parts of Phragmites communis stem, and 100-150 parts of Benincasa hispida seeds.

2. The traditional Chinese medicine composition for treating bronchial obstruction in chickens according to claim 1, characterized in that, The traditional Chinese medicine composition is prepared from the following raw materials in the following weight proportions: 100 parts of Isatis root, 40 parts of Houttuynia cordata, 40 parts of Lepidium apetalum, 33 parts of Citrus reticulata peel, 12 parts of Mentha haplocalyx, 20 parts of Stemona japonica, 30 parts of Arctium lappa, 14 parts of Curcuma longa, 28 parts of Glycyrrhiza uralensis, 120 parts of Eucalyptus globulus, 5 parts of Borneol, 5 parts of Glauber's salt, 8 parts of Pinellia ternata, 180 parts of Phragmites communis stem, and 100 parts of Benincasa hispida seeds.

3. A traditional Chinese medicine composition for treating bronchial obstruction in chickens according to any one of claims 1 to 2, characterized in that, The traditional Chinese medicine composition is weighed according to the specified weight proportions, pulverized and sieved separately, then mixed evenly to prepare a traditional Chinese medicine powder for animal use.

4. A method for preparing a traditional Chinese medicine composition for treating bronchial obstruction in chickens according to any one of claims 1 to 2, comprising the following preparation steps: (1) Take four medicinal materials: houttuynia cordata, mint, eucalyptus leaves and winter melon seeds, place them in an extraction tank, add water equivalent to 15 to 30 times the weight of the medicinal materials, soak for 0.5 to 2 hours, heat to boiling, keep boiling for 6 to 12 hours, collect the volatile oil to obtain volatile oil A; filter the extract to obtain extract B. (2) Take five herbs: Stemona japonica, Glycyrrhiza uralensis, Glauber's salt, Pinellia ternata, and Phragmites communis. Place them in an extraction tank and add water equivalent to 6 to 10 times the weight of the herbs. Reflux and extract 1 to 3 times, 1 to 3 hours each time. Combine the extracts and concentrate them together with extract B under reduced pressure to a clear paste with a relative density of 1.10 to 1.15 at 55°C. Cool the paste and add an appropriate amount of ethanol to make the alcohol content of the solution reach 40 to 75%. Let it stand, filter, and obtain the supernatant C. (3) Take five herbs: Isatis root, Lepidium seed, Citrus reticulata peel, Arctium lappa seed, and Curcuma longa. Place them in an extraction tank and add ethanol of 6 to 10 times the weight of the herbs and a volume fraction of 40% to 75%. Reflux and extract 1 to 3 times, 0.5 to 2 hours each time. Combine the extracts and remove the ethanol from the supernatant C under reduced pressure until there is no alcohol smell. Continue to concentrate under reduced pressure to a thick paste with a relative density of 1.21 to 1.25 at 55°C. Dry at 40 to 100°C and pulverize to obtain the support raw material D.

5. The application of the traditional Chinese medicine composition for treating bronchial obstruction in chickens according to any one of claims 1 to 2 in the preparation of a traditional Chinese medicine preparation for treating bronchial obstruction in chickens, characterized in that, The traditional Chinese medicine preparations mentioned are traditional Chinese medicine powders and traditional Chinese medicine oral liquids.

6. The application according to claim 5, characterized in that, The preparation process of the traditional Chinese medicine powder is as follows: (1) Weigh each raw material according to the above weight proportions, and prepare volatile oil A and plugging raw material D according to the preparation method in claim 4; (2) Take 5 to 10 times the mass of volatile oil A of β-cyclodextrin, add 15 to 30 times the mass of cyclodextrin of purified water, and stir evenly at 25 to 50°C; (3) Take volatile oil A and dissolve it in ethanol with an amount equivalent to 1 to 3 times the mass of volatile oil A. Under stirring conditions, slowly add it dropwise into the cyclodextrin solution. After the dropwise addition is complete, stir for another 30 minutes, filter, dry the precipitate at low temperature until dry, and pulverize it to obtain inclusion complex E. (4) Take borneol, crush it, and mix it evenly with the plugging raw material D and the inclusion compound E to make a plugging powder.

7. The application according to claim 5, characterized in that, The preparation process of the oral liquid of traditional Chinese medicine is as follows: (1) Weigh each raw material according to the above weight proportions, and prepare volatile oil A and plugging raw material D according to the preparation method in claim 4; (2) Take volatile oil A and borneol, add 5-30% of the total volume of ethanol, stir to dissolve, and obtain ethanol solution F; (3) Take the raw material D for bronchodilator, place it in a liquid preparation tank, add 50% of the total volume of purified water, stir to dissolve, add ethanol solution F, stir evenly, refrigerate for 24 hours, filter, add purified water to make up the volume, mix evenly, and make bronchodilator oral solution.

8. The application according to claim 5, characterized in that, The traditional Chinese medicine preparation has the effects of clearing heat and detoxifying, cooling blood and ventilating the lungs, relieving cough and asthma. It is used to treat bronchial obstruction in chickens and is suitable for use in the later stage of broiler breeding and during the egg-laying period of laying hens.

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