Application of Apple MdIAMT Gene in Improving Anthocyanin Content in Apple

By cloning and overexpressing the MdIAMT gene in apples, IAA homeostasis was regulated, and the anthocyanin content in apple fruits was successfully improved, which solved the problem of unclear regulation mechanism of apple anthocyanin content, and had application value to improve the color of the fruit.

CN119040390BActive Publication Date: 2025-03-25SHANDONG AGRICULTURAL UNIVERSITY
0 Cites 0 Cited by

Patent Information

Application Number
CN202411505382.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-10-28
Publication Date
2025-03-25
Estimated Expiration
2044-10-28

AI Technical Summary

Technical Problem

The specific regulatory genes and regulatory mechanisms of apple anthocyanin content are not yet clear, making it difficult to increase the anthocyanin content in apple fruits.

Method used

By mining the SABATH methylase family sequence MdIAMT in the Apple genome database, the primers were designed to successfully clone the homologous gene MdIAMT, the expression vector was constructed and the apple callus and fruit were transformed, and the MdIAMT gene was overexpressed to regulate IAA homeostasis and promote the accumulation of anthocyanins.

Benefits of technology

The content of anthocyanins in callus and fruits of overexpressing MdIAMT transgenic apples increased significantly, while the content of anthocyanins in the fruits silencing this gene decreased, indicating that the MdIAMT gene positively regulates the accumulation of anthocyanins in apples by regulating the IAA homeostasis and improves the color of the fruit.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention discloses application of an apple MdIAMT gene in improving anthocyanin content in apples, and relates to the technical field of screening apple anthocyanin-related genes. The invention constructs an expression vector, utilizes a genetic transformation method to transfer the MdIAMT gene into apple callus tissue and transiently infects apple fruit, and the anthocyanin content of the apple callus tissue and apple fruit overexpressing MdIAMT is increased, while the anthocyanin content of the apple fruit silencing the MdIAMT gene is reduced. In addition, the content of inactive auxin in the apple callus tissue overexpressing MdIAMT is increased, indicating that MdIAMT affects anthocyanin accumulation by regulating IAA homeostasis. The gene has the potential to be applied to gene editing to cultivate new varieties with high anthocyanin content, and is of great significance to the directional genetic improvement of apple color.
Need to check novelty before this filing date? Find Prior Art