Management system for biological tissue samples

Abstract

The application relates to a biological tissue sample management system, which comprises a preservation room, an image acquisition component and a processing module, the preservation room comprises a first storage space; the image acquisition component is used for acquiring images; the processing module is used for: collecting a first image of a biological tissue sample in the preservation room before the biological tissue sample is placed in the first storage space of the preservation room for the first time; if the position of a structural feature in the first image is different from the position of a corresponding feature in a library atlas, and / or the appearance of the structural feature is different from the appearance of the corresponding feature in the library atlas, marking the first image; and placing the biological tissue sample corresponding to the marked first image into the first storage space. In the management system, the biological tissue sample will not face the risk of repeated freezing and thawing, the marked first image is convenient for subsequent inspection and sampling, the marking mode is efficient and scientific, and manual cost can be saved.

Description

Technical Field

[0001] This invention relates to the field of biological tissue sample management system technology, and in particular to a biological tissue sample management system. Background Technology

[0002] Taking the human brain as an example, the brain is the most complex and mysterious organ in the human body. Billions of neurons form an extremely intricate network of connections, and the complex information transmission between brain regions enables the brain to process and store vast amounts of information, while also endowing humans with abilities such as thinking, perception, emotion, and memory. However, our understanding of the brain and brain diseases remains very limited. Neuropsychiatric disorders themselves possess highly complex clinical characteristics and lack objective diagnostic criteria and biomarkers. Based on meticulous and detailed clinical assessments, and closely combined with objective clinical characteristics, post-mortem brain tissue banks of neuropsychiatric disease patients will become the key to unlocking the mysteries of neuropsychiatric diseases, greatly advancing the development of research on these disorders.

[0003] Currently, the standardized operating procedures of the Chinese Brain Tissue Bank have specified requirements for the collection, processing, and preservation of human brain tissue. However, these procedures are still largely performed manually by senior anatomy or pathology experts based on years of experience, requiring a high level of technical expertise and representing a significant barrier to achieving standardized collection, processing, and preservation across multiple centers nationwide. Furthermore, this method presents risks such as inconvenient management, high labor costs, difficulty in processing sample sections, poor section quality, failure to promptly freeze and preserve samples, and repeated freeze-thaw cycles during collection, leading to waste. Summary of the Invention

[0004] The problem the invention aims to solve

[0005] To address at least one of the aforementioned technical problems, this disclosure provides a management system for biological tissue samples.

[0006] Solution for solving the problem

[0007] The first aspect of this disclosure provides a method for managing biological tissue samples, the method comprising:

[0008] Before the biological tissue sample is placed into the first storage space of the preservation room for the first time, a first image of the biological tissue sample is acquired in the preservation room; wherein the temperature of both the preservation room and the first storage space is lower than a preset temperature, and the first image includes at least one structural feature of the biological tissue sample; the structural feature is used to compare with a library of images to obtain a comparison result; the comparison result includes: the position of the structural feature in the first image is the same as or different from the position of the structural feature in the library of images; and / or, the morphology of the structural feature is the same as or different from the morphology of the corresponding feature in the library of images;

[0009] If the position of the structural feature in the first image is different from the position of the corresponding feature in the library atlas; and / or, the morphology of the structural feature is different from the morphology of the corresponding feature in the library atlas, the first image is marked;

[0010] The biological tissue sample corresponding to the labeled first image is placed into the first storage space.

[0011] Optionally, before placing the biological tissue sample into the first storage space for the first time, the method further includes:

[0012] The biological tissue sample is assigned storage information; the storage information includes at least one of the following: the name of the biological tissue sample, and the location information of the biological tissue sample in the first storage space of the preservation room.

[0013] Optionally, the method further includes:

[0014] According to the sampling instruction, the location information of the target biological tissue sample corresponding to the sampling instruction is determined; wherein, the location information includes at least the location information of the target biological tissue sample within the first storage space;

[0015] Based on the positioning information, the control mechanism retrieves the target biological tissue sample from the first storage space.

[0016] Optionally, the sampling instruction includes: a target biological tissue sample and a target structural feature; wherein the target structural feature is at least one structural feature of the target biological tissue sample;

[0017] The method further includes:

[0018] Under video surveillance or the visualization window of the storage room, the conveying mechanism is controlled to extract the target structural feature from the target biological tissue sample; wherein, the target structural features are different, and the drill bits installed on the conveying mechanism for material extraction are of the same or different sizes.

[0019] Optionally, the method further includes:

[0020] Before placing the biological tissue sample into the first storage space for the Nth time, a second image of the biological tissue sample is acquired; wherein, N is a positive integer greater than or equal to 2;

[0021] If the second image of the same biological tissue sample is different from the first image, then the first image is updated to the second image;

[0022] The biological tissue sample is placed into the first storage space for the Nth time.

[0023] A second aspect of this disclosure provides a management system for biological tissues, the management system comprising:

[0024] The preservation room includes at least a first storage space for storing the biological tissue sample;

[0025] An image acquisition component, used for acquiring images; and a processing module, communicatively connected to the image acquisition component, used for:

[0026] Before the biological tissue sample is placed into the first storage space of the preservation room for the first time, a first image of the biological tissue sample is acquired in the preservation room; wherein the temperature of both the preservation room and the first storage space is lower than a preset temperature, and the first image includes at least one structural feature of the biological tissue sample; the structural feature is used to compare with a library of images to obtain a comparison result; the comparison result includes: the position of the structural feature in the first image is the same as or different from the position of the structural feature in the library of images; and / or, the morphology of the structural feature is the same as or different from the morphology of the corresponding feature in the library of images;

[0027] If the position of the structural feature in the first image is different from the position of the corresponding feature in the library atlas; and / or, the morphology of the structural feature is different from the morphology of the corresponding feature in the library atlas, the first image is marked;

[0028] The biological tissue sample corresponding to the labeled first image is placed into the first storage space.

[0029] The processing module is also used for:

[0030] The biological tissue sample is assigned storage information; the storage information includes at least one of the following: the name of the biological tissue sample, and the location information of the biological tissue sample in the first storage space.

[0031] The processing module is also used for:

[0032] According to the sampling instruction, the location information of the target biological tissue sample corresponding to the sampling instruction is determined; wherein, the location information includes at least the location information of the target biological tissue sample within the first storage space;

[0033] Based on the positioning information, the control mechanism retrieves the target biological tissue sample from the first storage space.

[0034] The sampling instruction includes: a target biological tissue sample and a target structural feature; wherein, the target structural feature is at least one structural feature of the target biological tissue sample;

[0035] The processing module is also used for:

[0036] Under video surveillance or the visualization window of the storage room, the conveying mechanism is controlled to extract the target structural feature from the target biological tissue sample; wherein, the target structural features are different, and the drill bits installed on the conveying mechanism for material extraction are of the same or different sizes.

[0037] The processing module is also used for:

[0038] Before placing the biological tissue sample into the first storage space for the Nth time, a second image of the biological tissue sample is acquired; wherein, N is a positive integer greater than or equal to 2;

[0039] If the second image of the same biological tissue sample is different from the first image, then the first image is updated to the second image;

[0040] The biological tissue sample is placed into the first storage space for the Nth time.

[0041] The management system further includes: a conveying mechanism, which is a low-temperature conveying mechanism, or the conveying mechanism is located in the storage room and is communicatively connected to the processing module;

[0042] The processing module controls the transmission mechanism to place the biological tissue sample corresponding to the marked first image into the first storage space.

[0043] The biological tissue sample is a biological tissue slice, and the storage information also includes: the slice order of the biological tissue slice among all biological tissue slices.

[0044] Optionally, the management system further includes a plurality of carriers located in the storage chamber, each carrier carrying one slice of the biological tissue.

[0045] Optionally, the storage room is divided into multiple storage units, and each storage unit is provided with at least one of the carriers; when a storage unit is provided with at least two carriers, the at least two carriers are stacked from bottom to top.

[0046] Optionally, the carrier is a transparent component; and / or, the carrier is a sheet-like structure.

[0047] Optionally,

[0048] The conveying mechanism includes: an inbound conveying mechanism and an outbound conveying mechanism;

[0049] The inbound conveying mechanism is used at least to convey the biological tissue slices from the slicing mechanism to the storage room; the outbound conveying mechanism is used to convey the biological tissue slices in the first storage space to the outbound sampling station, wherein the outbound sampling station is located in the storage room and outside the first storage space.

[0050] Optionally, the management system further includes: a slicing mechanism.

[0051] The slicing mechanism includes a slicing mold and a blade. The slicing mold is used to hold the biological tissue, and the blade is used to slice the biological tissue.

[0052] The slicing mold includes a receiving cavity and multiple relief grooves, wherein the receiving cavity is used to receive the biological tissue;

[0053] The plurality of relief grooves are arranged side by side along a first direction, and each relief groove penetrates the slicing mold along a second direction, so as to slice the biological tissue located in the receiving cavity when the blade is inserted into the relief groove; wherein the second direction intersects the first direction;

[0054] The blade can be inserted into the relief groove to slice the biological tissue located within the receiving cavity.

[0055] Optionally, the slicing mold further includes:

[0056] An opening is provided, which communicates with the receiving cavity, through which the biological tissue enters the receiving cavity.

[0057] Optionally, the depth of each of the clearance grooves along a third direction is greater than or equal to the depth of the biological tissue along the third direction, and less than the depth of the slicing mold along the third direction, wherein the third direction is perpendicular to the first direction.

[0058] Optionally, the clearance groove has an inclination angle greater than 0° with respect to the third direction.

[0059] Optionally, the slicing mold has a cuboid outline, the opening is located on the top surface of the slicing mold, each of the relief grooves extends through opposite sides of the slicing mold along a second direction, and the first direction is perpendicular to the second square.

[0060] Optionally, the slicing mold is made of a transparent, semi-transparent, or opaque material; and / or, the material of the slicing mold has anti-tissue adhesion properties.

[0061] Optionally, the distance between two adjacent clearance grooves is equal, so that the thickness of the plurality of biological tissue slices is equal.

[0062] Optionally, the slicing mold includes at least two sub-molds, which are detachably connected. Each sub-mold has a sub-cavity, and the at least two sub-cavities are joined together to form the receiving cavity.

[0063] Optionally, the biological tissue includes: brain tissue, heart tissue, liver tissue, lung tissue, intestinal tissue, kidney tissue, muscle tissue, solid tumor or cancerous tissue.

[0064] Optionally, the slicing mechanism further includes a loading mechanism, wherein the slicing mold is detachably mounted on the loading mechanism.

[0065] Optionally, the preservation chamber includes at least a first storage space and a second storage space; the biological tissue slices are stored in the first storage space; the slicing mechanism is located in the second storage space; and the temperature of the preservation chamber is lower than a preset temperature to provide a low-temperature or ultra-low-temperature environment.

[0066] The effects of the invention

[0067] In this embodiment, an image of the biological tissue sample is acquired before its first storage, resulting in a first image. This first image is then labeled using a stored image database. After labeling, the biological tissue sample is then stored. This management method compares the first image with the stored image database. The physical biological tissue sample can be kept at a low temperature in the storage room, avoiding the risk of repeated freeze-thaw cycles and ensuring the quality of the biological tissue sample. Moreover, the labeled first image facilitates subsequent inspection and sampling. This labeling method is efficient and scientific, and helps save labor costs. Attached Figure Description

[0068] Figure 1 This is a schematic diagram of the slicing mold structure in some embodiments of this disclosure;

[0069] Figure 2 This is a schematic diagram of the slicing mold in some other embodiments of this disclosure;

[0070] Figure 3 for Figure 2 Side view of the slicing mold;

[0071] Figure 4 for Figure 3 Another structural diagram of the slicing mold;

[0072] Figure 5 for Figure 2 Schematic diagram of the neutron mold;

[0073] Figure 6 This is a schematic diagram of the slicing mechanism in some embodiments of this disclosure;

[0074] Figure 7 This is a partial structural diagram of the slice structure in some embodiments of this disclosure;

[0075] Figure 8 This is one of the structural diagrams of the management system in some embodiments of this disclosure;

[0076] Figure 9 This is a second schematic diagram of the structure of the management system in some embodiments of this disclosure;

[0077] Figure 10 This is one of the schematic diagrams illustrating the process of calibrating images corresponding to biological tissue slices in some embodiments of this disclosure;

[0078] Figure 11 This is the second schematic diagram illustrating the process of calibrating images corresponding to biological tissue slices in some embodiments of this disclosure;

[0079] Figure 12 This is a schematic diagram of the structure of the conveying mechanism when collecting biological tissue slices in some embodiments of this disclosure.

[0080] Explanation of reference numerals in the attached figures

[0081] 100. Slicing mold; 110. Receiving cavity; 120. Relief groove; 130. Sub-mold; 140. Opening; 160. Mounting hole;

[0082] 200. Biological tissue section; 210. Structural features; 220. First image;

[0083] 300. Slicing mechanism; 310. Slicing chamber; 320. Loading mechanism;

[0084] 400. Blade;

[0085] 500. Management system; 510. Storage room; 520. Conveying mechanism; 521. Sampling drill bit; 531. Storage unit; 532. Supporting component; 540. Outbound sampling platform; 550. Image acquisition component; 560. Intelligent device;

[0086] 600. Library atlas; 610. Corresponding features of library atlases and biological tissue sections. Detailed Implementation

[0087] To make the technical solutions and beneficial effects of the embodiments of this disclosure more apparent and understandable, a detailed description is provided below by listing specific embodiments. The accompanying drawings are not necessarily drawn to scale, and local features may be enlarged or reduced to more clearly show the details of the local features; unless otherwise defined, the technical and scientific terms used herein have the same meanings as those in the technical field to which this application pertains.

[0088] In the description of the embodiments of this disclosure, the terms "width", "thickness", "height", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the purpose of simplifying the description of the embodiments of this disclosure and are not intended to indicate that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. That is, they should not be construed as limitations on the embodiments of this disclosure.

[0089] In the embodiments of this disclosure, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating the relative importance of the indicated features or the number of indicated technical features. Therefore, a feature defined as "first" or "second" can explicitly include at least one of that feature. In the description of the embodiments of this disclosure, "multiple" means at least two, such as two, three, etc.; "several" means at least one, such as one, two, three, etc., unless otherwise explicitly specified.

[0090] In the embodiments of this disclosure, unless otherwise expressly defined, the terms "installation," "connection," "linking," "fixing," "setting," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral part; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can also refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of this disclosure according to the specific circumstances.

[0091] In the embodiments of this disclosure, unless otherwise expressly defined, the terms "above," "on top of," "over," "above," "below," "below," or "below" for "first feature over second feature" can refer to the first and second features being in direct contact, or to the first and second features being in indirect contact through an intermediate medium. Furthermore, "above," "over," and "below" for "first feature over second feature" can mean the first feature is directly above or diagonally above the second feature, or simply indicates that the horizontal height of the first feature is higher than the horizontal height of the second feature. Similarly, "below," "below," and "below" for "first feature over second feature" can mean the first feature is directly below or diagonally below the second feature, or simply indicates that the horizontal height of the first feature is lower than the horizontal height of the second feature.

[0092] Example 1

[0093] This disclosure provides a biological tissue slicing mold, such as... Figures 1 to 4 As shown, the slicing mold 100 includes: at least one receiving cavity 110 and a plurality of relief grooves 120, the receiving cavity 110 being used to receive biological tissue; the plurality of relief grooves 120 being arranged side by side along a first direction, each relief groove 120 penetrating the slicing mold 100 along a second direction, so that when the blade 400 is inserted into the relief groove 120, the biological tissue slice 200 located in the receiving cavity 110 is sliced; wherein, the second direction intersects the first direction.

[0094] The second direction is related to the functional location of the corresponding biological tissue. For example, multiple functional locations distributed along a certain direction in the human brain are key areas of focus in clinical practice and research. In such cases, a suitable direction can be chosen as the second direction to expose more of these locations after cutting. The first direction can be perpendicular to the second direction, but it is not limited to this.

[0095] exist Figure 1 In the implementation shown, the slice module is roughly rectangular in shape. The first direction can refer to the length direction, and the second direction is the width direction, which is perpendicular to the first direction. However, it is not limited to this.

[0096] For example, Figure 1 The outer frame dimensions of the slicing mold 100 shown are: length 200-240mm, width 200-240mm, height 150-180mm.

[0097] The receiving cavity 110 provides a certain degree of containment for the biological tissue, reducing tissue movement during slicing. The receiving cavity 110 is generally customized to the biological tissue to be processed; for example, the receiving cavity 110 must meet at least one of the following conditions: the inner wall of the receiving cavity 110 conforms as closely as possible to the outer wall of the biological tissue; and the shape of the receiving cavity 110 is approximately the same as the shape of the biological tissue.

[0098] For biological tissues that cannot withstand pressure or have weak compressive capacity, such as the human brain, the inner wall of the receiving cavity 110 will not exert any compressive force or exert very little compressive force on the biological tissue. For biological tissues with strong compressive capacity, the inner wall of the receiving cavity 110 can exert greater compressive force on the biological tissue to improve the fixation effect of the receiving cavity 110 on the biological tissue.

[0099] Figure 1 In the illustrated embodiment, the slicing mold 100 is used to slice the human brain, and the receiving cavity 110 is approximately spherical, matching the shape of the human brain. There is almost no gap between the inner wall of the receiving cavity 110 and the outer wall of the human brain.

[0100] There must be at least two clearance slots 120. The number of clearance slots 120 can be determined according to the number of slices to be cut; the more slices, the more clearance slots 120 are required.

[0101] In this embodiment, the biological tissue can be stably placed within the receiving cavity 110. After the blade 400 is inserted into the relief groove 120, it can cut the biological tissue within the receiving groove. The multiple relief grooves 120 can meet the requirements for biological tissue slices 200. Furthermore, since the relief groove 120 has a fixed and reliable size, and the biological tissue is confined within the receiving groove, the blade 400 will not cause poor slice quality due to changes in the cutting position during cutting. This slicing mold 100 has low dependence on the operator's experience during the processing of biological tissue slices 200, eliminating the need for experienced anatomy or pathology experts, making the processing of biological tissues simpler and more convenient.

[0102] In some embodiments, such as Figure 5 As shown, the slicing mold 100 also includes an opening 140, which is connected to the receiving cavity 110, through which biological tissue enters the receiving cavity 110.

[0103] If the slicing mold 100 is a one-piece structure and does not require splicing, then the opening 140 can be exposed, such as... Figure 1 As shown, the slicing mold 100 has a cuboid outline, with an opening 140 located on the top surface of the slicing mold 100, and each clearance groove 120 extending through the opposite sides of the slicing mold 100 along the second direction.

[0104] If the slicing mold 100 comprises at least two parts joined together, the opening 140 may be at least exposed or not exposed at all.

[0105] like Figure 2 As shown, the slicing mold 100 includes at least two sub-molds 130, which are detachably connected. Each sub-mold 130 has a sub-cavity, and the at least two sub-cavities are joined together to form a receiving cavity 110. Figure 2The slicing mold 100 shown is composed of two sub-molds 130 joined together. In this non-integral structure, the opening 140 is located on each sub-mold 130. After the two sub-molds 130 are joined together, they can form an appearance roughly as shown. Figure 1 The mold shape shown here is such that the exposed opening 140 is only a portion of the opening 140 of the sub-mold 130.

[0106] At least two sub-molds 130 are joined together to form a slicing mold 100, which facilitates the removal of biological tissue and provides better fixation for it. For example, when biological tissue needs to be placed into the receiving cavity 110, at least two sub-molds 130 are disassembled and separated. The biological tissue is first placed into the cavity of at least one sub-mold 130, and then the other sub-molds 130 are fixed in place. During the process of fixing the other sub-molds 130, the connection force between the sub-molds 130 can be strengthened to increase the compressive force on the biological tissue and improve the fixation effect of the receiving cavity 110 on the biological tissue.

[0107] Figure 2 The sub-mold 130 shown has a hemispherical cavity. In addition to splicing two sub-molds 130 to form a complete spherical cavity 110 for cutting the whole brain, it is also possible to use only one sub-mold 130 to contain the hemisphere and slice it.

[0108] In this embodiment of the disclosure, biological tissues include human tissues or animal tissues. For example, biological tissues include, but are not limited to, brain tissue, heart tissue, liver tissue, lung tissue, intestinal tissue, kidney tissue, muscle tissue, solid tumor tissue, or cancerous tissue.

[0109] The biological tissue in this embodiment can be fresh tissue or non-fresh tissue. For example, the biological tissue can be a non-fresh fixed tissue sample.

[0110] In some embodiments, such as Figure 1 and Figure 2 As shown, the distance between two adjacent clearance grooves 120 is equal, so that the thickness of multiple biological tissue slices 200 is equal.

[0111] The slicing mold 100 of this embodiment can more accurately and scientifically extract biological tissue in equal portions, reduce waste, and also facilitate the standardized production of biological tissue slices 200.

[0112] In some embodiments, such as Figure 1 and Figure 2 As shown, the depth of each clearance groove 120 along the third direction is greater than or equal to the depth of the biological tissue along the third direction, and less than the depth of the slicing mold 100 along the third direction, wherein the third direction is perpendicular to the first direction.

[0113] exist Figure 1 In this context, the third direction can refer to the height direction.

[0114] The depth of the clearance groove 120 along the third direction determines the cutting depth of the blade 400. The depth of the clearance groove 120 along the third direction is greater than or equal to the depth of the biological tissue along the third direction to ensure that the blade 400 can completely sever the biological tissue. The depth of each clearance groove 120 along the third direction is less than the depth of the slicing mold 100 along the third direction to ensure that the blade 400 does not penetrate the slicing mold 100 and damage other components outside the slicing mold 100.

[0115] In some embodiments, the clearance groove 120 has an inclination angle greater than 0° with a third direction. The tilted clearance groove 120 can expose the desired biological tissue functional location to ensure that the cut brain slices can match the functional location of the human brain, the Atlas of the Human Brain, and the magnetic resonance imaging section.

[0116] For example, the angle of inclination between the clearance groove 120 and the third direction is 15°-35°.

[0117] The tilt angle between the clearance groove 120 and the third direction can be determined based on parameters matching the functional location characteristics of the human brain, the atlas of human brain tissue, and the magnetic resonance imaging cross-section. For example: in Figure 1 and Figure 2 In the implementation shown, the tilt angle between the clearance groove 120 and the third direction is 20° to ensure that the cut brain slices can match the functional position of the human brain, the human brain tissue atlas and the magnetic resonance imaging section.

[0118] In some embodiments, the slicing mold 100 satisfies at least one of the following conditions: the slicing mold 100 is made of a transparent, translucent, or opaque material; the material of the slicing mold 100 has anti-tissue adhesion properties; the slicing mold 100 is a mold that has undergone pre-cooling treatment.

[0119] Transparent or semi-transparent slicing molds make it easier to navigate the slicing process. If an error occurs during slicing, it can be stopped at any time, which helps improve the quality of the slices.

[0120] The material used to prevent tissue adhesion can be similar to that used in cryovials. For example, the material of the sectioning mold 100 includes, but is not limited to, polypropylene (PP), polyethylene (PE), and polyvinylidene fluoride (PVDF).

[0121] Pre-cooling refers to placing the slicing mold 100 in a lower temperature environment for a period of time before slicing, so that the slices 200 of soft and fragile biological tissues such as human brain are easier to slice. For example, the slicing mold 100 is pre-cooled at an environment of 2℃-8℃ for a period of time.

[0122] In some implementations, the slicing mold 100 and the biological tissue can be pre-cooled simultaneously, for example, by washing and pre-cooling the biological tissue with ice-cold saline. Alternatively, the slicing mold 100 can be pre-cooled without pre-cooling the biological tissue, or the slicing mold 100, blade 400, and biological tissue can all be placed in a low-temperature environment for slicing.

[0123] In a specific example, the slicing mold 100 is used to slice a human brain. For example... Figure 3 As shown, the outer frame dimensions of the slicing mold 100 are approximately 260mm * 200mm * 180mm. By dividing the wide side in half, two sub-molds 130 are obtained, each approximately 260mm long, 120mm wide, and 180mm high. The inclination angle of the clearance groove 120 is 20° (a smooth arc inclination angle, inclined along the long side). The material of the slicing mold 100 is preferably transparent and prevents tissue adhesion during cryopreservation. The distance between two adjacent clearance grooves 120 is 5mm. The brain tissue, from which the cerebellum and brainstem tissue have been removed, is placed in the mold along the bottom edge parallel to the mold, so that the coronal section is consistent with the coronal section of the functional magnetic resonance imaging. Subsequently, a midline section is positioned and sliced ​​along the plane behind the mammary body. After fixing the position of the frontal and occipital lobes and placing the blade 400, a uniform thickness section is sliced. After slicing, the biological tissue section 200 is carefully and completely removed, flattened, and placed for the next processing step.

[0124] To facilitate the splicing and fixing of different sub-molds 130, such as Figure 3 and Figure 4 As shown, each sub-mold 130 has at least two mounting holes 160 at its bottom. Fasteners can be inserted into the corresponding mounting holes 160 of two adjacent sub-molds 130 to merge and fix them to form a complete slicing mold 100.

[0125] The mounting hole 160 is located below the relief groove 120 and between the bottom surface of the slicing mold 100 to avoid the mounting hole 160 affecting the slicing operation.

[0126] Example 2

[0127] like Figure 6 and Figure 7 As shown, this embodiment of the present disclosure also provides a slicing mechanism 300 including: a slicing mold 100 and a blade 400, the blade 400 being able to be inserted into a relief groove 120 to slice biological tissue 200 located in a receiving cavity 110.

[0128] The slicing mold 100 can be any of the slicing molds 100 described in Example 1, or other slicing molds 100 can be used.

[0129] like Figure 6 As shown, there can be one blade 400. One blade 400 is inserted into multiple clearance slots 120 in sequence to achieve slicing.

[0130] Alternatively, the slicing mechanism 300 may have two or more blades 400. Figure 7 As shown in the figure, this allows multiple blades 400 to be inserted into multiple corresponding clearance slots 120 simultaneously to slice the tissue at the same time, thereby improving the processing efficiency of biological tissues.

[0131] The slicing action of the blade 400 can be performed by a robotic arm, or the slicing action of the blade 400 can be performed by a drive mechanism with lifting or horizontal reciprocating movement functions.

[0132] In some embodiments, such as Figure 6 As shown, the slicing mechanism 300 includes: a slicing chamber 310, which at least accommodates a slicing mold 100 and a blade 400; the temperature of the slicing chamber 310 is lower than a preset temperature to provide a low-temperature or ultra-low-temperature environment for biological tissue slicing operations.

[0133] The preset temperature can be 8℃, 10℃, 15℃, 20℃, 25℃, etc. Low temperature or ultra-low temperature is lower than the preset temperature, with ultra-low temperature being lower than the low temperature. For example, the low temperature can be 2℃-8℃, or around 20℃.

[0134] The slicing chamber 310 can at least provide a suitable temperature environment for slicing operations to facilitate the slicing process.

[0135] Understandably, the slide chamber 310 also provides a clean and hygienic environment for slide operations, reducing contamination of biological tissues and ensuring the quality of biological tissue slides.

[0136] In some embodiments, such as Figure 6 and Figure 7 As shown, the slicing mechanism 300 also includes a loading mechanism 320, to which the slicing mold 100 is detachably mounted. The loading mechanism 320 can serve as a worktable for slicing operations. For example, the loading mechanism 320 is used to at least support the slicing mold 100, and the blade 400 can be inserted from top to bottom into the relief groove 120 to slice the biological tissue 200.

[0137] The detachable slicing mold 100 can flexibly handle complex sampling environments. For example, if a low-temperature or ultra-low-temperature environment is available, slicing can be performed in this environment without removing the slicing mold 100 from the loading mechanism 320, ensuring that the biological tissue sections 200 are preserved quickly and with high quality. If conditions are limited, the slicing mold 100 can be detached separately, and slicing can be performed quickly outside the slicing chamber 310, followed by transport to the subsequent processing environment via a cryogenic transport system such as a vehicle-mounted refrigerator.

[0138] In some embodiments, the slicing mechanism 300 further includes a clamp that can be connected to the slicing mold 100 to fix the slicing mold 100. For some biological tissues, which are soft and require less cutting force, the stability of the biological tissue during slicing can be ensured by the weight of the slicing mold 100 itself and the limiting effect of the receiving cavity 110, and the clamp may not be used for such biological tissues. However, for some biological tissues with high rigidity, which require greater cutting force, using a clamp to fix the slicing mold 100 can effectively ensure the stability of the slicing process for such tissues.

[0139] The fixture can be structured as a jaw, with multiple jaws clamping the slicing mold 100 circumferentially. Alternatively, the fixture can include a lifting pressure head. When the slicing mold 100 needs to be fixed, the lifting pressure head descends and abuts against the top of the slicing mold 100. Since the slicing mold 100 is placed on the loading mechanism 320, the slicing mold 100 can be clamped and fixed between the lifting pressure head and the loading mechanism 320 in the third direction.

[0140] For example, the slicing mold 100 is similar to a ruler groove clamping plate, and the clearance groove 120 is a dividing clamping groove, which can evenly divide and clamp the clamping arm part of the cutting tool (i.e. the blade), so that the cutting tool can obtain sample slices with uniform thickness during the process of cutting biological tissue. Before slicing biological tissue samples, a movable clamping plate (similar to the aforementioned lifting pressure head) can be set at the upper end of the slicing mold 100 to ensure that the biological tissue can be fixed during the cutting process, so as to ensure the stability of the slicing sampling.

[0141] Understandably, the structure of the fixture is not limited to this.

[0142] Example 3

[0143] like Figure 8 As shown in the embodiments of this disclosure, a biological tissue management system 500 is also provided. The management system 500 includes a slicing mechanism 300 and a storage and preservation mechanism. The storage and preservation mechanism includes at least a preservation room 510, which is used to store biological tissue slices 200 after slicing.

[0144] The slicing mechanism 300 can be any of the slicing mechanisms 300 described in Embodiment 2, or other slicing mechanisms 300 can be used.

[0145] The preservation room 510 is able to provide a suitable temperature environment for biological tissues so that biological tissue sections 200 can be preserved for a long time.

[0146] Understandably, the preservation room 510 can also provide a clean and hygienic environment for the biological tissue slides 200, reduce the contamination of the biological tissue slides 200, and ensure the quality of the biological tissue slides 200.

[0147] In some embodiments, the slicing mechanism 300 is also located within the storage chamber 510. For example, the storage chamber 510 includes a first storage space and a second storage space; the slicing mechanism 300 is located in the first storage space; and the second storage space is used to store biological tissue slices 200. In this way, the biological tissue slices 200 can be placed into the second storage space for preservation in a timely and rapid manner, further improving the quality of the biological tissue slices 200.

[0148] In some embodiments, the storage facility also includes a plurality of carriers 532 located within the storage chamber 510, each carrier 532 carrying a biological tissue slice 200.

[0149] The carrier 532 can be used to fix the biological tissue slices 200 and also prevent the biological tissue slices 200 from sticking together during the preservation process.

[0150] Without limitation, the carrier 532 may be a transparent element; and / or, the carrier 532 may be a sheet-like structure. For example, the carrier 532 may be a thin sheet of frost-resistant transparent material.

[0151] like Figure 8 and Figure 9 As shown, in some embodiments, the storage chamber 510 is divided into multiple storage units 531, each storage unit 531 having at least one support member 532; when a storage unit 531 has at least two support members 532, the at least two support members 532 are stacked from bottom to top. This distribution method facilitates the determination of the location of each piece of biological tissue, thereby facilitating subsequent operations such as sampling and storage of biological tissue sections 200.

[0152] In some embodiments, the management system 500 further includes a conveying mechanism 520, which is a cryogenic conveying mechanism 520, or both the conveying mechanism 520 and the slicing mechanism 300 are located within the preservation chamber 510. By setting up the conveying mechanism 520, the biological tissue slices 200, transfer, and preservation are all carried out in one device, enabling timely rapid freezing and preservation of biological tissues, reducing the risk of repeated freeze-thaw cycles, and making the entire process easier to control scientifically and precisely.

[0153] Without limitation, the cryogenic conveying mechanism 520 may have a quick-freezing function.

[0154] In this embodiment of the disclosure, the conveying mechanism 520 is not a cryogenic conveying mechanism 520. Both the conveying mechanism 520 and the slicing mechanism 300 are located in the preservation chamber 510. The preservation chamber 510 is divided into at least a first storage space, a second storage space, and a third storage space. As mentioned above, the first storage space is used to accommodate the slicing mechanism 300, the second storage space is used to preserve biological tissue slices 200, and the third storage space is used to accommodate the conveying mechanism 520.

[0155] Alternatively, the slicing mechanism 300 may not be located within the storage chamber 510. Instead, the biological tissue slices can be transferred to the storage chamber 510 using the transfer mechanism 520. In this case, the container holding the biological tissue slices 200 in the low-temperature transfer mechanism 520 can be a container capable of raising the low-temperature or ultra-low-temperature environment, thereby ensuring that the biological tissue slices 200 are in a low-temperature or ultra-low-temperature state during the transfer process, thus guaranteeing the slice quality.

[0156] The transfer structure is used to transfer the carrier 532 to at least transfer the biological tissue slice 200 from the slicing mechanism 300 to the second storage cavity.

[0157] In some embodiments, the conveying mechanism 520 includes an inbound conveying mechanism 520 and an outbound conveying mechanism 520; wherein, the inbound conveying mechanism 520 is at least used to convey the biological tissue slices 200 from the slicing mechanism 300 to the storage room 510 for storage (hereinafter referred to as the inbound operation); the outbound conveying mechanism 520 is used to convey the biological tissue slices 200 in the storage room 510 to the outbound sampling station 540 (hereinafter referred to as the outbound operation). This conveying mechanism 520 can further ensure that the sampling process of biological samples is also in a low temperature or ultra-low temperature buffer, further reducing the risk of repeated freeze-thaw cycles of biological tissue during the sampling process.

[0158] The sampling station 540 can be located inside the storage room 510, outside the first storage space. Optionally, biological sample slides that have been taken and entered into the sampling station 540 can also be stored in a low-temperature or ultra-low-temperature environment.

[0159] Without limitation, both the inbound and outbound conveying mechanisms can employ robotic arms with gripping structures such as claws or suction cups. The claws or suction cups are used to fix the carrier 532 to facilitate the transfer of the biological tissue slices 200.

[0160] Figure 8 In the diagram, the dashed lines indicate the state of the transmission mechanism 520 at different locations.

[0161] In some embodiments, the retrieval and delivery mechanisms can be combined into one, i.e., the same delivery mechanism 520 is used to realize the storage and retrieval of biological tissue slices 200. For example, the retrieval and delivery mechanisms refer to the same robotic arm, which performs the storage operation of biological tissue slices 200 at different times (corresponding to...). Figure 8 Operations of conveyor mechanisms 520(A) to 520(B) or outbound operations (corresponding to...) Figure 8 Operation of transmission mechanisms 520(A) to 520(C).

[0162] In some embodiments, such as Figure 9 As shown, the management system 500 also includes an image acquisition component 550 and a processing module.

[0163] The image acquisition component 550 includes a camera.

[0164] Without limitation, the image acquisition component 550 may be built into the storage chamber 510 and perform image acquisition after the biological tissue section 200 is completed and before the section is transferred to the storage chamber 510.

[0165] The image acquisition component 550 is used to acquire images of the biological tissue slice 200 to obtain the structural features of the biological tissue slice 200. The processing module can perform image processing on the images of the biological tissue slice 200 to record the structural information of the biological tissue slice 200 for subsequent viewing.

[0166] The processing module can also determine the storage information for the image of the biological tissue slice 200. The storage information can at least determine "what" and "where" the biological tissue slice 200 is. "What" includes, but is not limited to: Is the biological tissue slice 200 a brain slice or a muscle tissue slice? Do the biological tissue slices 200 come from the same cadaver? What information is available about that cadaver? "Where" includes, but is not limited to: The location of the biological tissue slice 200 in the storage room 510, for example: On which unit and which carrier 532 of the storage room 510 is the biological tissue slice 200 located? Therefore, the storage information can be used to easily locate the required part for the user, and also facilitates the management and maintenance of the biological tissue slice 200.

[0167] In some embodiments, the processing module of the aforementioned management system 500 is at least used for:

[0168] Before placing the biological tissue sample into the first storage space of the preservation room for the first time, a first image of the biological tissue sample is acquired in the preservation room; wherein, the temperature of both the preservation room and the first storage space is lower than a preset temperature, and the first image includes at least one structural feature of the biological tissue sample; the structural feature is used to compare with a library of images to obtain a comparison result; the comparison result includes: the position of the structural feature in the first image is the same as or different from the position of the structural feature in the library of images; and / or, the morphology of the structural feature is the same as or different from the morphology of the corresponding feature in the library of images;

[0169] If the position of the structural feature in the first image is different from the position of the corresponding feature in the library; and / or, the morphology of the structural feature is different from the morphology of the corresponding feature in the library, the first image is marked.

[0170] Place the biological tissue sample corresponding to the first labeled image into the first storage space.

[0171] Before the biological tissue sample is first stored in the library, an image is acquired to obtain the first image. This first image is then labeled using existing images in the library. After labeling, the biological tissue sample is then stored. This management method compares the first image with existing images in the library. The physical biological tissue sample can be maintained at a low temperature in the storage room, avoiding the risk of repeated freeze-thaw cycles and ensuring the quality of the biological tissue sample. Moreover, this labeling method is efficient and scientific, helping to save labor costs.

[0172] By marking, each biological tissue sample can be recorded more accurately, which facilitates the subsequent accurate location of the target structural features of a biological tissue sample, making the collection and examination of the target structural features more convenient and efficient.

[0173] The temperature in the preservation chamber is at least suitable for the temporary preservation of biological tissues. The temperature in the primary storage space is suitable for the long-term preservation of biological tissues. Therefore, the temperature in the preservation chamber can be equal to or higher than the temperature of the primary storage space.

[0174] Unrestricted, the preset temperature can be 8℃, 10℃, 15℃, 20℃, 25℃, etc.

[0175] In this embodiment, the biological tissue sample processed by the processing module can be the aforementioned biological tissue slice, or it can be block-shaped biological tissue or whole biological tissue. However, it is not limited to these.

[0176] For ease of description, in this embodiment, the biological tissue sample processed by the processing module is illustrated using a biological tissue slice as an example.

[0177] For example, if the position of a structural feature in the first image is the same as the position of the structural feature in the database, and the shape of the structural feature is the same as the shape of the corresponding feature in the database, then there is no need to modify the database.

[0178] If the position of the structural feature in the first image is different from the position of the structural feature in the library map, or if the appearance of the structural feature is different from the morphology of the corresponding feature in the library map, then the first image needs to be marked. The difference between the first image and the library map is reflected in the marked information, thereby achieving the purpose of calibrating the first image.

[0179] For example, Figure 10 and Figure 11 The first images 220 of two different biological tissue slices 200 and their corresponding database atlases 600 are illustrated respectively. In the database atlas 600, the corresponding features 610 marked with circles have corresponding structural features 210 in the first image 220. Due to disease or individual differences, the same structural features of different biological tissue slices 200 may also differ. To more clearly determine the feature correspondence, the database atlas 600 can be referenced to mark the position of the structural feature 210 corresponding to the corresponding feature 610 at the corresponding position in the first image 220. The range of the structural feature or other information can also be marked.

[0180] The comparison of structural features 210 in the first image with corresponding features in the database can be performed entirely by the processing module or with manual intervention to improve the accuracy of the first image verification. Without limitation, the processing module in this embodiment can be a processor or chip of a smart device 560 such as a computer. The image acquisition component 550 can interact with the smart device 560 via wired or wireless communication, allowing the image acquisition component 550 to send the acquired first and second images to the smart device 560.

[0181] Optionally, the processing module controls the conveying mechanism to place the biological tissue sample corresponding to the marked first image into the first storage space. Since the conveying mechanism is a low-temperature conveying mechanism 520, and both the conveying mechanism 520 and the slicing mechanism 300 are located within the storage chamber 510, the slicing of biological tissue samples, the movement of biological tissue samples in different positions within the storage chamber, and the sampling can all be carried out in a low-temperature or ultra-low-temperature environment, reducing the risk of repeated freeze-thaw cycles of biological tissue samples, and making the entire process easier to control scientifically and precisely.

[0182] In some embodiments, the processing module is further configured to: determine storage information for biological tissue samples; the storage information includes at least one of the following: the name of the biological tissue sample, and the location information of the biological tissue sample in the first storage space of the preservation room.

[0183] If the biological tissue sample is a biological tissue slice, the entry information also includes: the slice order of the biological tissue slice among all biological tissue slices.

[0184] The information on the entry into the database facilitates the management of multiple biological tissue samples and also makes subsequent material collection easier.

[0185] In some embodiments, the processing module is further configured to:

[0186] Based on the sampling instruction, determine the location information of the target biological tissue sample corresponding to the sampling instruction; wherein, the location information includes at least the location information of the target biological tissue sample within the first storage space;

[0187] Based on the location information, the control and transmission mechanism retrieves the target biological tissue sample from the first storage space.

[0188] Material retrieval instructions can be received by the input module of the smart device 560. For example, material retrieval instructions can be entered into the keyboard of the smart device 560, or they can be received via voice or other means.

[0189] In some embodiments, the sampling instruction includes: a target biological tissue sample and a target structural feature; wherein the target structural feature is at least one structural feature of the target biological tissue sample;

[0190] The processing module is also used to: control the conveying mechanism to extract target structural features from the target biological tissue sample under the visualization window of the video monitoring or storage room; wherein, the target structural features are different, and the drill bit 521 installed on the conveying mechanism for material extraction is the same or different in size.

[0191] Different material-taking drill bits 521 can be replaced automatically by the conveying mechanism or manually.

[0192] In some application scenarios, each biological tissue slice 200 can be photographed sequentially and labeled accordingly. Labeling is not limited to determining the storage information for the biological tissue slice 200 in software; the storage information can also be marked on the physical carrier 532.

[0193] In some embodiments, the processing module is further configured to:

[0194] Before placing the biological tissue sample into the first storage space for the Nth time, a second image of the biological tissue sample is acquired; where N is a positive integer greater than or equal to 2.

[0195] If the second image of the same biological tissue sample is different from the first image, then the first image is updated to the second image;

[0196] The biological tissue sample is placed into the first storage space for the Nth time.

[0197] N can be 2, 3, 4, 5 or more.

[0198] Sampling may involve extracting only a portion of tissue corresponding to one or a few structural features. The extracted or removed tissue sample can be used for subsequent paraffin block preparation or pathological staining. The remaining tissue sample can be returned to the primary storage space. During this process, since the returned tissue sample has changed, the primary image needs to be updated.

[0199] like Figure 12 As shown, the material retrieval process can be carried out in the storage room using a conveyor mechanism.

[0200] In this embodiment, operations such as slicing, transferring, image acquisition, and sampling of biological tissues can all be performed in the low-temperature or ultra-low-temperature environment of the preservation room. Biological tissue samples can be repeatedly moved in and out of the first storage space (i.e., repeated freezing and thawing) without the problem of repeated freeze-thaw cycles. Furthermore, combined with the above structure, sample slicing is simple, slicing quality is high, and samples can be rapidly frozen and preserved in a timely manner. This reduces or even avoids the risks of repeated freeze-thaw cycles and waste encountered during sampling.

[0201] If the second image of the same biological tissue sample is the same as the first image, then there is no need to update the first image.

[0202] For example, when it is necessary to collect materials, the target biological tissue slice 200 can be first retrieved and identified on the smart device 560. Then, the location information in the storage information of the target biological tissue slice 200 can be obtained. Subsequently, the control conveying mechanism 520 is used to transfer the target biological slice to the sampling station 540 according to the location information, and the smart device 560 records that the target biological tissue slice 200 has been taken out, which is convenient for subsequent management.

[0203] Location information includes, but is not limited to, the coordinates or address of the biological tissue slice 200 in the storage room 510 (specifically, the second storage space in the storage room 510), so as to uniquely identify the corresponding biological tissue slice 200 from the location information.

[0204] In this embodiment of the disclosure, at least the transmission mechanism 520, the image acquisition component 550, and the processing module participate in forming an automated digital biological tissue sampling mechanism (also known as an automated digital biological tissue acquisition, preservation, and sampling system), making the collection of biological tissues simpler and more efficient.

[0205] For example, taking brain tissue as an example, the operational procedures involved in an automated digital biological tissue sampling facility are further illustrated. These procedures include, but are not limited to:

[0206] S110 is equipped with a high-definition camera, either upright or inverted, to take 200 photographs of biological tissue sections. The resulting images are automatically matched with the Atlas of the Human Brain in the system, manually calibrated, and then labeled and stored. Each gross section can be photographed sequentially, and its order and location information can be marked.

[0207] Due to individual differences, there may be differences between the actual tissue and the images in the library. The overall position and size of the biological tissue slices 200 in the library and the corresponding brain regions (such as structural features 210) can be scaled and adjusted to achieve the maximum match with the actual sample photos through automatic identification by the image processing software system and manual calibration. The position of each slice and the brain regions and structural features 210 it contains are labeled, numbered and the information is entered into the library.

[0208] S120. Gross sections of human brain tissue are stacked and stored on an internal cryopreservation rack according to certain rules (same donor, same site, etc.), allowing for automated slide picking and sample handling. During this process, 200 samples of biological tissue sections can be fixed in a paraffin box or tray and transferred using a transfer mechanism 520, then immersed in tissue fixative for preservation at room temperature or 2-8°C. Fresh tissue can be preserved long-term at ultra-low temperature or deep cryogenic environments.

[0209] S130. Based on the user's needs, find the corresponding donor and the corresponding brain region slices, and mark the area to be sampled on the Atlas of the Human Brain in the system.

[0210] The sampling command is sent to the software management system, which controls the built-in robotic arm (i.e., the conveyor mechanism 520 located in the storage chamber 510) to accurately extract samples. Specifically, the robotic arm is used for precise sampling, and the appropriate size sampling drill can be selected automatically or manually. The entire process is conducted under video monitoring or can be manually calibrated and corrected through a visual window. Depending on the sample source and experimental requirements, fresh tissue cryopreserved samples can be extracted in ultra-low temperature or deep low temperature environments; fixed sample brain slices can be extracted at room temperature or in an environment of 2-8 degrees Celsius.

[0211] The sampling site is automatically marked, manually verified, and recorded upon release. The retrieved sample is placed on a tissue-like paraffin block box or tray, which can be pre-marked with its location, information, and corresponding QR code. Both the retrieved tissue and the gross sections obtained after sampling are photographed and recorded for subsequent verification.

[0212] In summary, this embodiment of the present disclosure, based on the actual needs of biological tissue processing, preservation, and sampling, and according to the morphological characteristics of biological tissues and parameters matching their functional location characteristics, biological tissue atlases, and magnetic resonance imaging sections, designs a more precise and scientific aliquoting mold 100. Combined with an automated sample storage system and a digital tissue chip system, this forms an automated digital biological tissue collection, preservation, and sampling system. This system offers comprehensive advantages, including the fastest possible cryopreservation of human brain tissue, standardized production of gross sections of human brain tissue, digital image information storage and preservation, and accurate positioning and sampling of biological tissue sections 200.

[0213] Example 4

[0214] Based on the foregoing description, this embodiment provides a method for managing biological tissue samples, the method comprising:

[0215] S10. Before placing the biological tissue sample into the first storage space of the preservation room for the first time, a first image of the biological tissue sample is acquired in the preservation room; wherein, the temperature of both the preservation room and the first storage space is lower than a preset temperature, and the first image includes at least one structural feature of the biological tissue sample; the structural feature is used to compare with the library map to obtain a comparison result; the comparison result includes: the position of the structural feature in the first image is the same as or different from the position of the structural feature in the library map; and / or, the morphology of the structural feature is the same as or different from the morphology of the corresponding feature in the library map;

[0216] S20. If the position of the structural feature in the first image is different from the position of the corresponding feature in the library; and / or, the morphology of the structural feature is different from the morphology of the corresponding feature in the library, the first image is marked.

[0217] S30. Place the biological tissue sample corresponding to the marked first image into the first storage space.

[0218] In some embodiments, before the biological tissue sample is first placed into the first storage space, the method further includes:

[0219] The biological tissue sample is assigned storage information; the storage information includes at least one of the following: the name of the biological tissue sample, and the location information of the biological tissue sample in the first storage space of the preservation room.

[0220] In some embodiments, the method further includes:

[0221] According to the sampling instruction, the location information of the target biological tissue sample corresponding to the sampling instruction is determined; wherein, the location information includes at least the location information of the target biological tissue sample within the first storage space;

[0222] Based on the positioning information, the control mechanism retrieves the target biological tissue sample from the first storage space.

[0223] In some embodiments, the sampling instruction includes: a target biological tissue sample and a target structural feature; wherein the target structural feature is at least one structural feature of the target biological tissue sample;

[0224] In some embodiments, the method further includes:

[0225] Under video surveillance or the visualization window of the storage room, the conveying mechanism is controlled to extract the target structural feature from the target biological tissue sample; wherein, the target structural features are different, and the drill bits installed on the conveying mechanism for material extraction are of the same or different sizes.

[0226] In some embodiments, the method further includes:

[0227] Before placing the biological tissue sample into the first storage space for the Nth time, a second image of the biological tissue sample is acquired; wherein, N is a positive integer greater than or equal to 2;

[0228] If the second image of the same biological tissue sample is different from the first image, then the first image is updated to the second image;

[0229] The biological tissue sample is placed into the first storage space for the Nth time.

[0230] The structure, principle, and beneficial effects of the biological tissue sample management method in this embodiment can be referred to the foregoing description, and will not be repeated here.

[0231] Without conflict, different embodiments or different technical features of this disclosure can be arbitrarily combined to form new embodiments.

[0232] It should be understood that the above embodiments are exemplary and are not intended to encompass all possible implementations included in the claims. Various modifications and changes can be made to the above embodiments without departing from the scope of this disclosure. Similarly, the various technical features of the above embodiments can be arbitrarily combined to form other embodiments of the present invention that may not be explicitly described. Therefore, the above embodiments only illustrate several implementations of the present invention and do not limit the scope of protection of this patent.

Claims

1. A management system for biological tissue samples, characterized in that, The management system includes: The preservation room includes a first storage space and a second storage space, wherein the first storage space is used to store the biological tissue sample; A slicing mechanism, comprising a slicing mold and a blade, wherein the slicing mold is used to hold the biological tissue and the blade is used to slice the biological tissue, and the slicing mechanism is located in the second storage space; A conveying mechanism is located in the storage room and is communicatively connected to the processing module; An image acquisition component, wherein the image acquisition component is used to acquire images; The processing module is communicatively connected to the image acquisition component, and the processing module is used for: Before the biological tissue sample is placed into the first storage space of the preservation room for the first time, a first image of the biological tissue sample is acquired in the preservation room; wherein the temperature of both the preservation room and the first storage space is lower than a preset temperature, and the first image includes at least one structural feature of the biological tissue sample; the structural feature is used to compare with a library of images to obtain a comparison result; the comparison result includes: the position of the structural feature in the first image is the same as or different from the position of the structural feature in the library of images; and / or, the morphology of the structural feature is the same as or different from the morphology of the corresponding feature in the library of images; If the position of the structural feature in the first image is different from the position of the corresponding feature in the library atlas; and / or, the morphology of the structural feature is different from the morphology of the corresponding feature in the library atlas, the first image is marked; The transmission mechanism is controlled to place the biological tissue sample corresponding to the marked first image into the first storage space; According to the sampling instruction, determine the location information of the target biological tissue sample corresponding to the sampling instruction; according to the location information, control the conveying mechanism to retrieve the target biological tissue sample from the first storage space; Under video surveillance or the visualization window of the storage room, the conveying mechanism is controlled to extract the target structural features from the target biological tissue sample; wherein, the target structural features are different, and the drill bits installed on the conveying mechanism for material extraction are of the same or different sizes.

2. The management system according to claim 1, characterized in that, The processing module is also used for: The biological tissue sample is assigned storage information; the storage information includes at least one of the following: the name of the biological tissue sample, and the location information of the biological tissue sample in the first storage space.

3. The management system according to claim 1 or 2, characterized in that, The location information includes at least the location information of the target biological tissue sample within the first storage space.

4. The management system according to claim 3, characterized in that, The sampling instruction includes: a target biological tissue sample and a target structural feature; wherein the target structural feature is at least one structural feature of the target biological tissue sample.

5. The management system according to claim 1, characterized in that, The processing module is also used for: Before placing the biological tissue sample into the first storage space for the Nth time, a second image of the biological tissue sample is acquired; wherein, N is a positive integer greater than or equal to 2; If the second image of the same biological tissue sample is different from the first image, then the first image is updated to the second image; The biological tissue sample is placed into the first storage space for the Nth time.

6. The management system according to claim 2, characterized in that, The biological tissue sample is a biological tissue slice, and the storage information also includes: the slice order of the biological tissue slice among all biological tissue slices; The management system also includes multiple carriers located in the storage chamber, each carrier carrying one slice of the biological tissue; The storage room is divided into multiple storage units, and each storage unit is provided with at least one of the carriers; when a storage unit is provided with at least two carriers, the at least two carriers are stacked from bottom to top. The carrier is a transparent component; and / or the carrier is a sheet-like structure.

7. The management system according to claim 6, characterized in that, The conveying mechanism includes: an inbound conveying mechanism and an outbound conveying mechanism; The inbound conveying mechanism is used at least to convey the biological tissue slices from the slicing mechanism to the storage room; the outbound conveying mechanism is used to convey the biological tissue slices stored in the first storage space of the storage room to the outbound sampling station, wherein the outbound sampling station is located in the storage room and outside the first storage space.

8. The management system according to any one of claims 6 to 7, characterized in that, The slicing mold includes a receiving cavity and multiple relief grooves, wherein the receiving cavity is used to receive the biological tissue; The plurality of relief grooves are arranged side by side along a first direction, and each relief groove penetrates the slicing mold along a second direction, so as to slice the biological tissue located in the receiving cavity when the blade is inserted into the relief groove; wherein the second direction intersects the first direction; The blade can be inserted into the relief groove to slice the biological tissue located within the receiving cavity.

9. The management system according to claim 8, characterized in that, The slicing mold further includes an opening communicating with the receiving cavity, through which the biological tissue enters the receiving cavity; The depth of each of the clearance grooves along the third direction is greater than or equal to the depth of the biological tissue along the third direction, and less than the depth of the slicing mold along the third direction, wherein the third direction is perpendicular to the first direction; The clearance groove has an inclination angle greater than 0° with the third direction; The slicing mold has a cuboid outline, the opening is located on the top surface of the slicing mold, each of the relief grooves extends through opposite sides of the slicing mold along a second direction, and the first direction is perpendicular to the second direction; the slicing mold is made of a transparent, semi-transparent, or opaque material; and / or, the material of the slicing mold has anti-tissue adhesion properties; The distance between two adjacent clearance grooves is equal, so that the thickness of the plurality of biological tissue slices is equal; The slicing mold includes at least two sub-molds, which are detachably connected. Each sub-mold has a sub-cavity, and the at least two sub-cavities are joined together to form the receiving cavity.

10. The management system according to claim 8, characterized in that, The biological tissues include: brain tissue, heart tissue, liver tissue, lung tissue, intestinal tissue, kidney tissue, muscle tissue, solid tumor or cancerous tissue.

11. The management system according to claim 8, characterized in that, The slicing mechanism further includes a loading mechanism, wherein the slicing mold is detachably mounted on the loading mechanism.

Images