A beta-caryophyllene synthase and its use in the preparation of beta-caryophyllene

CN119060994BActive Publication Date: 2026-06-16GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
Filing Date
2024-09-06
Publication Date
2026-06-16

AI Technical Summary

⚠Technical Problem

Existing technologies make it difficult to efficiently and on a large scale obtain β-caryophyllene. Traditional methods of extraction from plants are limited and chemical synthesis is complex. Microbial cell factory production has potential but has not been fully utilized.

⚗Method used

By constructing a high-yield β-caryophyllene-producing Saccharomyces cerevisiae engineered strain, β-caryophyllene synthase was expressed in the engineered strain, and farnesyl pyrophosphate was used as a substrate to produce β-caryophyllene. The strain was then expressed by fusion of ERG20 and AarTPS88 with a flexible linker, and HIF-1, UPC2-1 and tHMGR genes were overexpressed to optimize the MVA pathway and improve metabolic flux.

🎯Benefits of technology

A high-yield β-caryophyllene engineered strain was successfully obtained, with a shake flask yield of 828.4 mg/L and a β-caryophyllene titer of 15.6 g/L in the fermenter, reaching the highest level currently achieved in yeast.

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Abstract

The application discloses a beta-caryophyllene synthase and application thereof in preparation of beta-caryophyllene. The application is based on SE00 chassis fungus with high yield of precursor FPP, and ERG20 (FPP synthase) and AarTPS88 (beta-caryophyllene synthase) are fused and expressed through a flexible linker (GGGS) 2, and genes HIF-1, UPC2-1 and tHMGR are overexpressed to improve metabolic flux of the MVA pathway, so that the biosynthesis of beta-caryophyllene is improved. The engineering bacteria SE17 for producing beta-caryophyllene are successfully obtained, and the shake flask yield of beta-caryophyllene is 828.4 mg / L.
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