调控小菜蛾抵御蝉拟青霉侵染的基因、制备方法及其应用

By regulating the PGRP-LB and miR308-p3 genes of the diamondback moth and using dsRNA technology to modulate the diamondback moth's immune function, the problem of slow speed and unstable effect of fungal insecticides in controlling the diamondback moth was solved, achieving a highly efficient biological control effect.

CN119061019BActive Publication Date: 2026-07-17ANHUI AGRICULTURAL UNIVERSITY

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ANHUI AGRICULTURAL UNIVERSITY
Filing Date
2024-10-10
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing fungal insecticides are slow to kill and have unstable control effects when controlling diamondback moth. They are also easily affected by the environment. The immune mechanisms of insects against pathogenic fungal infection are not fully understood, resulting in unsatisfactory control effects.

Method used

By regulating the genes PGRP-LB and miR308-p3 in the diamondback moth, and using dsRNA to interfere with PGRP-LB expression or mimic/inhibit miR308-p3 expression, the immune function of the diamondback moth can be regulated, thereby enhancing its resistance to Paecilomyces cicadae.

Benefits of technology

It significantly improved the control effect of diamondback moth on Paecilomyces ciliata, reduced the survival rate of pests, reduced the development of drug resistance, and the preparation method can be mass-produced and has strong environmental adaptability.

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Abstract

本发明涉及生物技术领域,具体涉及调控小菜蛾抵御蝉拟青霉侵染的基因、制备方法及其应用,基因为miR308‑p3或PGRP‑LB,通过PCR扩增获得。同时提供了过表达或抑制miR308‑p3的制备方法以及干扰基因PGRP‑LB表达的dsRNA的制备方法。小菜蛾3龄幼虫注射PGRP‑LB基因的dsRNA后,PGRP‑LB基因的转录水平大幅减少。经蝉拟青霉侵染后小菜蛾存活率显著降低。将miR308‑p3的模拟物或抑制剂分别注射到小菜蛾的3龄幼虫后,PGRP‑LB的表达水平分别显著降低或升高,miR308‑p3能够负调控PGRP‑LB的表达。过表达miR308‑p3和抑制miR308‑p3的表达后的小菜蛾在蝉拟青霉侵染后存活率分别显著降低和升高。表明基因miR308‑p3通过负调控PGRP‑LB的表达进而参与到小菜蛾对蝉拟青霉侵染的免疫反应,这两个基因可应用到害虫防治中,能有效提高真菌杀虫剂防治效果。
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