A separation device and method for extracting and separating polysaccharides in perilla by using a double-water-phase extraction

Abstract

The application relates to the technical field of double-water-phase separation device production, in particular to a double-water-phase extraction and separation device and method for separating polysaccharides in perilla, which comprises a bottom plate, the upper end of the bottom plate is fixedly connected with a connecting column, the inner upper end of an arc-shaped plate is fixedly connected with a first sealing cover and a second sealing cover, two groups of liquid guide pipes are screwed in the inner upper end of the second sealing cover, the lower end of the liquid guide pipes extends out of the second sealing cover, and a liquid adding through hole is arranged in the inner upper end of the first sealing cover; water, an organic solution and perilla are introduced into corresponding connecting boxes by opening three groups of first electromagnetic valves; the supporting disc is driven by a hydraulic cylinder to move upwards; perilla residues are drawn out of a separation mixing pipe through a liquid discharge pipe; the drawn-out upper-phase liquid and lower-phase liquid are evaporated and dried to obtain an extract; the continuous separation and extraction of yellow sugar in perilla is realized; and the amount of water source and extraction solution to be added can be prepared and grasped.

Description

Technical Field

[0001] This invention relates to the field of aqueous two-phase separation device technology, specifically to a separation device and method for separating polysaccharides from perilla through aqueous two-phase extraction. Background Technology

[0002] Aqueous two-phase extraction is a commonly used chemical separation technique, typically used to extract and separate target compounds from mixed solutions. Its principle is based on the ability of two immiscible solvents, usually an aqueous phase and an organic phase, to separate into two layers. This technique is commonly used in fields such as biochemistry, drug preparation, and environmental analysis.

[0003] For example, Chinese patent CN216653469U discloses a two-phase extraction device for Polygonatum polysaccharides, including a mixing chamber and a separation chamber. A drive motor is fixedly installed in the middle of the top of the mixing chamber. A rotating shaft is rotatably connected to the middle of the top of the inner wall of the mixing chamber. Several first stirring rods are fixedly connected to the surface of the rotating shaft. An installation plate is fixedly connected between the first stirring rods. An installation hole is opened in the center of the installation plate. A heating tube arranged longitudinally with the mixing chamber is fixedly connected inside the installation hole. An electric heating tube is installed between the top and bottom of the inner wall of the heating tube.

[0004] However, the above-mentioned solution has the following shortcomings: The drive motor in the above-mentioned patent causes the first stirring rod, heating tube and the second stirring rod to rotate, thereby improving the mixing efficiency of the mixture. The pump body is started to draw the mixture in the mixing tank into the separation tank, and then the electric telescopic rod is controlled to push the separation to extract and separate the Polygonatum odoratum raw material to obtain high-quality Polygonatum odoratum extract. However, the above-mentioned patent cannot continuously extract brown sugar. After a set of materials is extracted, the operator needs to add materials, water and extraction solution again before the extraction of brown sugar in perilla can be carried out again. Moreover, it is impossible to accurately control the amount of water and extraction solution to be added each time. Therefore, we have introduced a separation device and method for separating polysaccharides in perilla by two-phase extraction. Summary of the Invention

[0005] The purpose of this invention is to provide a separation device and method for separating polysaccharides from perilla through aqueous two-phase extraction, so as to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] A separation device for separating polysaccharides from perilla using aqueous two-phase extraction includes a base plate. A connecting column is fixedly connected to the upper end of the base plate. A connecting ring is movably sleeved on the outside of the connecting column. Two sets of separation and mixing tubes are fixedly connected inside the connecting ring. A support plate is provided on the upper side of the connecting column. The lower end of the support plate is movably connected to the output end of a hydraulic cylinder. The hydraulic cylinder is fixedly installed inside the upper end of the connecting column. Two sets of arc-shaped plates are movably connected inside the support plate. A first sealing cover and a second sealing cover are fixedly connected inside the upper end of the arc-shaped plates. Two sets of liquid guide tubes are screwed into the upper end of the second sealing cover. The lower ends of the liquid guide tubes extend out of the second sealing cover. A liquid filling hole is opened inside the upper end of the first sealing cover. A first one-way valve is fixedly connected inside the liquid filling hole.

[0008] A stirring mechanism is movably connected inside the separating mixing tube. The lower end of the stirring mechanism extends out of the separating mixing tube and meshes with a second internal toothed ring. The second internal toothed ring is fixedly installed on the upper end of the base plate. A U-shaped support plate is fixedly connected to the upper end of the base plate. Several sets of connecting boxes are fixedly connected to the lower end of the U-shaped support plate. The connecting boxes are connected to the output end of the first solenoid valve. The first solenoid valve is fixedly connected inside the U-shaped support plate. The input end of the first solenoid valve is connected to a storage box. The storage box is fixedly connected to the upper end of the U-shaped support plate.

[0009] An adjusting plate is movably connected inside the connecting box. A T-shaped screw is screwed into the lower end of the connecting box. The upper end of the T-shaped screw is fixedly connected to the adjusting plate. A second solenoid valve is fixedly connected inside the adjusting plate. The output end of the second solenoid valve is fixedly connected to the telescopic tube. The end of the telescopic tube away from the adjusting plate is fixedly connected to the liquid filling pipe. The liquid filling pipe is fixedly connected to the output end of the telescopic cylinder. The telescopic cylinder is fixedly connected to the upper end of the U-shaped support plate.

[0010] A support column is fixedly connected to the lower end of the second sealing cover, a connecting cover is fixedly connected to the lower end of the support column, a filter plate is fixedly connected to the inner side of the connecting cover, a second one-way valve is fixedly connected to the upper end of the connecting cover, a drain pipe is fixedly connected to the support column, the drain pipe extends into the connecting cover and passes through the filter plate, and the upper end of the drain pipe extends into the external environment.

[0011] Preferably, a first internal gear ring is fixedly connected to the lower end of the connecting ring, the inner side of the first internal gear ring meshes with a first gear, the first gear is fixedly connected to the output end of the transmission motor, and the transmission motor is fixedly installed inside the connecting column.

[0012] Preferably, the arc-shaped plate slides within the guide groove, the guide groove is formed within the support plate, a toothed plate is fixedly connected to one side of the arc-shaped plate, the toothed plate meshes with a second gear, the second gear is fixedly connected to the output end of the connecting motor, and the connecting motor is fixedly connected within the support plate.

[0013] Preferably, the stirring mechanism includes a connecting shaft, which is movably connected to the separating mixing tube. The lower end of the connecting shaft extends out of the separating mixing tube and is fixedly connected to a connecting gear. The connecting gear meshes with a second internal gear ring. A T-shaped stirring rod is movably connected to the upper end of the connecting shaft.

[0014] Preferably, the T-shaped stirring rod is movably connected to the connecting cavity, which is located at the upper end of the connecting shaft. A first spring is fixedly connected inside the connecting cavity, and the other end of the first spring is fixedly connected to the T-shaped stirring rod.

[0015] Furthermore, to achieve the above objectives, the present invention also provides a separation method and a separation apparatus for separating polysaccharides in perilla using aqueous two-phase extraction, comprising:

[0016] S1. By storing water, organic solvent, and crushed perilla liquid in three sets of storage tanks, open the first solenoid valves of the three sets. At this time, water, organic solution, and perilla will enter the corresponding connecting tanks in sequence. After the connecting tanks are filled, close the first solenoid valves, start the drive motor to move the separation mixing tube to the lower side of the telescopic cylinder, open the telescopic cylinder to allow the liquid adding tube to enter the liquid adding through hole, and put water, organic solution, and crushed perilla into the separation mixing tube. After the liquid adding tube returns to its original position, turn on the drive motor to make the separation mixing tube rotate.

[0017] S2. When the connecting ring rotates, the stirring mechanism will start to rotate to stir the material in the separation mixing tube. After the mixing and separation are completed, the connecting ring stops rotating, and the hydraulic cylinder will drive the support plate to move upward, so that the first sealing cover will be separated from the upper end of the separation mixing tube. By adjusting the arc plate, the second sealing cover will be moved to the upper end of the separation mixing tube. The hydraulic cylinder will drive the support plate to move downward, and the connecting cover will enter the separation mixing tube to filter the perilla residue between the upper and lower phase liquids into the connecting cover and push it to the bottom of the separation mixing tube.

[0018] S3. By connecting the two sets of liquid guide tubes and the liquid drain tube to external equipment, the perilla residue is extracted from the separation and mixing tube through the liquid drain tube. The liquid in the lower phase is extracted first through one set of liquid guide tubes. After the lower phase liquid is extracted, the remaining upper phase liquid is extracted. After all the liquid and perilla residue are extracted from the separation and mixing tube, the first sealing cap is re-clamped on the top of the separation and mixing tube, and water, organic solution and perilla liquid are introduced into the tube again. The extracted upper and lower phase liquids are evaporated and dried to obtain the extract.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows: By opening the three sets of first solenoid valves, water, organic solution, and perilla enter the corresponding connecting boxes. By opening the telescopic cylinder, the liquid addition pipe enters the liquid addition through-hole, allowing water, organic solution, and crushed perilla to enter the separation mixing pipe. After the connecting boxes are emptied, the first solenoid valves are activated again, reconnecting the connecting boxes and storage boxes. The water, organic solution, and crushed perilla liquid in the storage box re-enter the connecting boxes. By activating the drive motor, the connecting ring drives the separation mixing pipe to rotate. When the connecting ring rotates, the stirring mechanism rotates, accelerating the separation speed of polysaccharides in the perilla liquid. After separation, the hydraulic cylinder drives the support plate to move upward, causing the second sealing cover to move to the upper end of the separation mixing tube. The connecting cover enters the separation mixing tube to filter the perilla residue. The perilla residue is then extracted from the separation mixing tube through the drain pipe. One set of liquid guide pipes extracts the liquid in the lower phase, while another set extracts the liquid in the upper phase. After all the liquid has been extracted, the first sealing cover is re-clamped onto the upper part of the separation mixing tube, and water, organic solution, and perilla liquid are introduced and discharged again. The extracted upper and lower phase liquids are evaporated and dried to obtain the extract, achieving continuous separation and extraction of brown sugar in perilla. The amount of water and extraction solution to be added is carefully controlled. Attached Figure Description

[0020] Figure 1 This is a schematic cross-sectional view of the present invention;

[0021] Figure 2 This is a schematic diagram of the three-dimensional structure of the separation and mixing tube of the present invention;

[0022] Figure 3 This is a cross-sectional structural diagram showing the connection between the support plate in the raised state and the second sealing cover of the present invention;

[0023] Figure 4 This is a three-dimensional structural diagram illustrating the connection relationship between the connecting disc and the arc-shaped plate of the present invention;

[0024] Figure 5 This is a schematic diagram of the main structure of the present invention;

[0025] Figure 6 This is a three-dimensional structural diagram illustrating the connection relationship between the arc-shaped plate and the first sealing cover of the present invention.

[0026] Figure 7 This is a cross-sectional view of the connecting cover of the present invention inside the separation mixing tube;

[0027] Figure 8 This is a cross-sectional view of the stirring mechanism of the present invention.

[0028] In the diagram: 1. Base plate; 2. Connecting cover; 3. Separating mixing tube; 4. First spring; 5. Support plate; 6. Liquid guide tube; 7. Connecting cavity; 8. Connecting column; 9. Connecting ring; 10. Second internal gear ring; 11. U-shaped support plate; 12. Telescopic cylinder; 13. Liquid filling tube; 14. Telescopic tube; 15. T-screw; 16. Connecting box; 17. Storage box; 18. First solenoid valve; 19. Second gear; 20. Second solenoid valve; 21. Adjustment. 21. Plate; 22. Liquid filling through hole; 23. Hydraulic cylinder; 24. Filter plate; 25. T-shaped stirring rod; 26. Connecting shaft; 27. Connecting gear; 28. First gear; 29. ​​Drive motor; 30. First check valve; 31. Arc plate; 32. Drain pipe; 33. First sealing cover; 34. Guide groove; 35. Second sealing cover; 36. Toothed plate; 37. Connecting motor; 38. Support column; 39. First internal toothed ring; 40. Second check valve. Detailed Implementation

[0029] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0030] Please see Figure 1-8 The present invention provides a technical solution:

[0031] Example 1:

[0032] A separation device for separating polysaccharides from perilla using aqueous two-phase extraction includes a base plate 1. A connecting column 8 is fixedly connected to the upper end of the base plate 1. A connecting ring 9 is movably sleeved on the outside of the connecting column 8. Two sets of separation mixing tubes 3 are fixedly connected inside the connecting ring 9. The rotation of the connecting ring 9 drives the two sets of separation mixing tubes 3 to rotate, so that the substances in the separation mixing tubes 3 are fully mixed. A support plate 5 is provided on the upper side of the connecting column 8. The lower end of the support plate 5 is movably connected to the output end of a hydraulic cylinder 23. The hydraulic cylinder 23 is fixedly installed inside the upper end of the connecting column 8. Two sets of arc-shaped plates 31 are movably connected inside the support plate 5. A first sealing cover 33 and a second sealing cover 35 are fixedly connected to the upper end of the arc-shaped plates 31. By opening the hydraulic cylinder 23, the support plate 5 is moved upward, causing the first sealing cover 33 or the second sealing cover 35 to disengage from the snap seal on the separation mixing tube 3. Two sets of liquid guide tubes 6 are screwed into the upper end of the second sealing cover 35, and the lower end of the liquid guide tubes 6 extends out of the second sealing cover 35. The height of the two sets of liquid guide tubes 6 is adjusted according to the position of the upper phase liquid and the lower phase liquid in the separation mixing tube 3, so that when separating polysaccharides in batches, the two sets of liquid guide tubes 6 can directly reach the positions of the upper phase liquid and the lower phase liquid. A liquid filling hole 22 is opened in the upper end of the first sealing cover 33, and a first one-way valve 30 is fixedly connected in the liquid filling hole 22.

[0033] A stirring mechanism is movably connected inside the separating mixing tube 3. The lower end of the stirring mechanism extends out of the separating mixing tube 3 and meshes with the second internal toothed ring 10. The second internal toothed ring 10 is fixedly installed on the upper end of the base plate 1. A U-shaped support plate 11 is fixedly connected to the upper end of the base plate 1. Several sets of connecting boxes 16 are fixedly connected to the lower end of the U-shaped support plate 11. The connecting boxes 16 are connected to the output end of the first solenoid valve 18. The first solenoid valve 18 is fixedly connected inside the U-shaped support plate 11. The input end of the first solenoid valve 18 is connected to the storage box 17. The storage box 17 is fixedly connected to the upper end of the U-shaped support plate 11. Water, organic solvent, and crushed perilla liquid are stored in three sets of storage boxes 17. After storage, the three sets of first solenoid valves 18 are opened. At this time, water, organic solution, and perilla will enter the corresponding connecting boxes 16 in sequence. After the connecting boxes 16 are filled, the first solenoid valves 18 are closed so that the connecting boxes 16 are not connected to the storage boxes 17.

[0034] An adjusting plate 21 is movably connected inside the connecting box 16. A T-shaped screw 15 is screwed into the lower end of the connecting box 16. The upper end of the T-shaped screw 15 is fixedly connected to the adjusting plate 21. By rotating the T-shaped screw 15, the adjusting plate 21 is moved up or down along the connecting box 16 to adjust the amount of water, organic solution, or crushed perilla liquid entering the connecting box 16. A second solenoid valve 20 is fixedly connected inside the adjusting plate 21. The output end of the second solenoid valve 20 is fixedly connected to the telescopic pipe 14. The end of the telescopic pipe 14 away from the adjusting plate 21 is fixedly connected to the liquid adding pipe 13. The liquid adding pipe 13 is fixedly connected to the output end of the telescopic cylinder 12. The telescopic cylinder 12 is fixedly connected to the upper end of the U-shaped support plate 11. When the liquid adding pipe 13 enters the liquid adding hole 22, several sets of second solenoid valves 20 are opened, so that water, organic solution, and crushed perilla sequentially enter the telescopic pipe 14, and then enter the separation mixing pipe 3 through the first one-way valve 30 from the telescopic pipe 14.

[0035] The lower end of the second sealing cover 35 is fixedly connected to a support column 38, the lower end of the support column 38 is fixedly connected to a connecting cover 2, the inner side of the connecting cover 2 is fixedly connected to a filter plate 24, the upper end of the connecting cover 2 is fixedly connected to a second one-way valve 40, the supporting column 38 is fixedly connected to a drain pipe 32, the drain pipe 32 extends into the connecting cover 2 and passes through the filter plate 24, the upper end of the drain pipe 32 extends into the external environment, the hydraulic cylinder 23 drives the support plate 5 to move downward, so that the connecting cover 2 enters the separation mixing tube 3, the filter plate 24 located in the connecting cover 2 filters the perilla residue set between the upper and lower phase liquids into the connecting cover 2 and pushes it to the bottom of the separation mixing tube 3, after the connecting cover 2 contacts the T-shaped stirring rod 25, the T-shaped stirring rod 25 will be pressed downward and moved, when the connecting cover 2 leaves the separation mixing tube 3, under the elastic force of the first spring 4, the T-shaped stirring rod 25 returns to its original position.

[0036] Example 2:

[0037] Based on Embodiment 1, in order to enable the connecting ring 9 to rotate and drive the two sets of separating mixing tubes 3 to rotate, and to allow the first sealing cover 33 and the second sealing cover 35 to switch back and forth at the upper end of the separating mixing tubes 3, a first internal gear ring 39 is fixedly connected to the lower end of the connecting ring 9. The inner side of the first internal gear ring 39 meshes with the first gear 28. The first gear 28 is fixedly connected to the output end of the drive motor 29, which is fixedly installed inside the connecting column 8. The drive motor 29 drives the first gear 28 to start rotating, the rotation of the first gear 28 drives the first internal gear ring 39 to rotate, and the rotation of the first internal gear ring 39 drives the connecting ring 9 to rotate. 9. The moving mechanism drives the separation mixing tube 3 to rotate. The arc plate 31 slides in the guide groove 34, which is opened in the support plate 5. A toothed plate 36 is fixedly connected to one side of the arc plate 31. The toothed plate 36 meshes with the second gear 19. The second gear 19 is fixedly connected to the output end of the connecting motor 37, which is fixedly connected in the support plate 5. The connecting motor 37 drives the second gear 19 to rotate. Since the second gear 19 meshes with the toothed plate 36, the arc plate 31 will move along the guide groove 34 during the rotation of the second gear 19, thereby switching the positions of the first sealing cover 33 and the second sealing cover 35 back and forth.

[0038] The stirring mechanism includes a connecting shaft 26, which is movably connected to the separating mixing tube 3. The lower end of the connecting shaft 26 extends out of the separating mixing tube 3 and is fixedly connected to a connecting gear 27, which meshes with a second internal gear ring 10. A T-shaped stirring rod 25 is movably connected to the upper end of the connecting shaft 26. The connecting ring 9 drives the separating mixing tube 3 to rotate. During the rotation, the connecting gear 27 moves along the second internal gear ring 10, causing the connecting shaft 26 to rotate. The rotation of the connecting shaft 26 drives the T-shaped stirring rod 25 to rotate. At the same time, as the connecting ring 9 drives the separating mixing tube 3 to rotate, the T-shaped stirring rod 25 also... The material in the separation mixing tube 3 is stirred to accelerate the separation speed of polysaccharides in the perilla liquid. The T-shaped stirring rod 25 is movably connected to the connecting cavity 7, which is located at the upper end of the connecting shaft 26. A first spring 4 is fixedly connected in the connecting cavity 7, and the other end of the first spring 4 is fixedly connected to the T-shaped stirring rod 25. When the upper end of the connecting cover 2 is at the upper end of the T-shaped stirring rod 25, the T-shaped stirring rod 25 will enter the connecting cavity 7 as the connecting cover 2 moves downward, causing the first spring 4 to be compressed. When the connecting cover 2 leaves the upper end of the T-shaped stirring rod 25, the T-shaped stirring rod 25 returns to its original position under the elastic force of the first spring 4.

[0039] Furthermore, to achieve the above objectives, the present invention also provides a separation method and a separation apparatus for separating polysaccharides in perilla using the above-mentioned aqueous two-phase extraction, comprising:

[0040] S1. By storing water, organic solvent, and crushed perilla liquid in three sets of storage tanks 17, and opening three sets of first solenoid valves 18, water, organic solution, and perilla will enter the corresponding connecting tanks 16 in sequence. After the connecting tanks 16 are filled, the first solenoid valves 18 are closed, and the drive motor 29 is started to move the separation mixing tube 3 to the lower side of the telescopic cylinder 12. The telescopic cylinder 12 is opened to allow the liquid adding tube 13 to enter the liquid adding through hole 22, and water, organic solution, and crushed perilla are added into the separation mixing tube 3. After the liquid adding tube 13 returns to its original position, the drive motor 29 is turned on to make the separation mixing tube 3 rotate.

[0041] S2. When the connecting ring 9 rotates, the stirring mechanism will start to rotate to stir the material in the separation mixing tube 3. After the mixing and separation are completed, the connecting ring 9 stops rotating, and the hydraulic cylinder 23 will drive the support plate 5 to move upward, so that the first sealing cover 33 is separated from the upper end of the separation mixing tube 3. By adjusting the arc plate 31, the second sealing cover 35 is moved to the upper end of the separation mixing tube 3. The hydraulic cylinder 23 drives the support plate 5 to move downward, and the connecting cover 2 enters into the separation mixing tube 3 to filter the perilla residue between the upper and lower phase liquids into the connecting cover 2 and pushes it to the bottom of the separation mixing tube 3.

[0042] S3. By connecting the two sets of liquid guide pipes 6 and the drain pipe 32 to external equipment, the perilla residue is extracted from the separation mixing tube 3 through the drain pipe 32. The liquid in the lower phase is extracted first through one set of liquid guide pipes 6. After the liquid in the lower phase is extracted, the remaining liquid in the upper phase is extracted. After all the liquid and perilla residue are extracted from the separation mixing tube 3, the first sealing cap 33 is re-clamped on the top of the separation mixing tube 3, and water, organic solution and perilla liquid are introduced into the tube again. The extracted upper and lower phase liquids are evaporated and dried to obtain the extract.

[0043] Working principle: During use, water, organic solvent, and crushed perilla liquid are stored in three sets of storage tanks 17. After storage, the first solenoid valves 18 of the three sets are opened. At this time, water, organic solvent, and perilla will enter the corresponding connecting tanks 16 in sequence. After the connecting tanks 16 are filled, the first solenoid valves 18 are closed to disconnect the connecting tanks 16 from the storage tanks 17. The drive motor 29 drives the first gear 28 to rotate. The rotation of the first gear 28 drives the first internal gear ring 39 to rotate. The rotation of the first internal gear ring 39 drives the connecting ring 9 to rotate. The movement of the connecting ring 9 moves the separating mixing tube 3 to the lower side of the telescopic cylinder 12. At this time, the telescopic cylinder 12 is opened, and the liquid adding tube 13 connected to its output end moves downward. The liquid adding tube 13 moves downward. The telescopic tube 14 is stretched. When the liquid filling tube 13 enters the liquid filling hole 22, several sets of second solenoid valves 20 are opened, allowing water, organic solution and crushed perilla to enter the telescopic tube 14 in sequence. Then, the telescopic tube 14 enters the separation mixing tube 3 through the first one-way valve 30. After all the water, organic solution and perilla in the three sets of connecting boxes 16 have entered the separation mixing tube 3, the telescopic cylinder 12 is controlled to make the liquid filling tube 13 return to its original position. At this time, the first solenoid valve 18 is activated again, so that the connecting box 16 and the storage box 17 are reconnected. The water, organic solution and crushed perilla liquid in the storage box 17 enter the connecting box 16 again for storage. When the connecting box 16 is full, the first solenoid valve 18 is closed again.

[0044] After water, organic solution, and pulverized perilla liquid have been added to both sets of separation mixing tubes 3, the drive motor 29 is turned on to drive the first gear 28 to rotate again, causing the connecting ring 9 to rotate the separation mixing tube 3. Simultaneously, during rotation, the connecting gear 27 moves along the second internal gear ring 10, causing the connecting shaft 26 to rotate. The rotation of the connecting shaft 26 drives the T-shaped stirring rod 25 to rotate. While the connecting ring 9 is rotating the separation mixing tube 3, the T-shaped stirring rod 25 also stirs the material in the separation mixing tube 3, accelerating the separation speed of polysaccharides in the perilla liquid. After mixing and separation are complete, the connecting ring 9 stops rotating. At this time, the hydraulic cylinder 23 drives the support plate 5 to move upward, causing the first sealing cover 33 to detach from the upper end of the separation mixing tube 3. The connecting motor 37 drives the second gear 19 to rotate. Since the second gear 19 meshes with the toothed plate 36, the arc plate 31 will move along the guide groove 34 during the rotation of the second gear 19. When the second sealing cover 35 moves to the upper end of the separation mixing tube 3, the connecting motor 37 is turned off, and the hydraulic cylinder 23 drives the support plate 5 to move downward, so that the connecting cover 2 enters the separation mixing tube 3. The filter plate 24 located in the connecting cover 2 filters the perilla residue set between the upper and lower phase liquids into the connecting cover 2 and pushes it to the bottom of the separation mixing tube 3. After the connecting cover 2 contacts the T-shaped stirring rod 25, the T-shaped stirring rod 25 will be pressed downward and moved. When the connecting cover 2 leaves the separation mixing tube 3, the T-shaped stirring rod 25 returns to its original position under the elastic force of the first spring 4.

[0045] By connecting the two sets of liquid guide tubes 6 and the drain tube 32 to external equipment, the perilla residue is extracted from the separation mixing tube 3 through the drain tube 32. The liquid in the lower phase is extracted first through one set of liquid guide tubes 6. After the lower phase liquid is extracted, the remaining upper phase liquid is extracted. After all the liquid and perilla residue are extracted from the separation mixing tube 3, the first sealing cap 33 is re-clamped on the top of the separation mixing tube 3, and water, organic solution and perilla liquid are introduced and discharged again. The extracted upper and lower phase liquids are evaporated and dried to obtain the extract.

[0046] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A separation device for separating polysaccharides from perilla using aqueous two-phase extraction, comprising a base plate, characterized in that: A connecting column is fixedly connected to the upper end of the base plate. A connecting ring is movably sleeved on the outside of the connecting column. Two sets of separation and mixing pipes are fixedly connected inside the connecting ring. A support plate is provided on the upper side of the connecting column. The lower end of the support plate is movably connected to the output end of the hydraulic cylinder. The hydraulic cylinder is fixedly installed inside the upper end of the connecting column. Two sets of arc-shaped plates are movably connected inside the support plate. A first sealing cover and a second sealing cover are fixedly connected inside the upper end of the arc-shaped plates. Two sets of liquid guide pipes are screwed into the upper end of the second sealing cover. The lower end of the liquid guide pipes extends out of the second sealing cover. A liquid filling hole is opened inside the upper end of the first sealing cover. A first one-way valve is fixedly connected inside the liquid filling hole. A stirring mechanism is movably connected inside the separating mixing tube. The lower end of the stirring mechanism extends out of the separating mixing tube and meshes with a second internal toothed ring. The second internal toothed ring is fixedly installed on the upper end of the base plate. A U-shaped support plate is fixedly connected to the upper end of the base plate. Several sets of connecting boxes are fixedly connected to the lower end of the U-shaped support plate. The connecting boxes are connected to the output end of the first solenoid valve. The first solenoid valve is fixedly connected inside the U-shaped support plate. The input end of the first solenoid valve is connected to a storage box. The storage box is fixedly connected to the upper end of the U-shaped support plate. An adjusting plate is movably connected inside the connecting box. A T-shaped screw is screwed into the lower end of the connecting box. The upper end of the T-shaped screw is fixedly connected to the adjusting plate. A second solenoid valve is fixedly connected inside the adjusting plate. The output end of the second solenoid valve is fixedly connected to the telescopic tube. The end of the telescopic tube away from the adjusting plate is fixedly connected to the liquid filling pipe. The liquid filling pipe is fixedly connected to the output end of the telescopic cylinder. The telescopic cylinder is fixedly connected to the upper end of the U-shaped support plate. A support column is fixedly connected to the lower end of the second sealing cover, a connecting cover is fixedly connected to the lower end of the support column, a filter plate is fixedly connected to the inner side of the connecting cover, a second one-way valve is fixedly connected to the upper end of the connecting cover, a drain pipe is fixedly connected to the support column, the drain pipe extends into the connecting cover and passes through the filter plate, and the upper end of the drain pipe extends into the external environment.

2. The separation device for separating polysaccharides from perilla using aqueous two-phase extraction according to claim 1, characterized in that: The lower end of the connecting ring is fixedly connected to a first internal gear ring, the inner side of which meshes with a first gear. The first gear is fixedly connected to the output end of the transmission motor, and the transmission motor is fixedly installed inside the connecting column.

3. The separation device for separating polysaccharides from perilla using aqueous two-phase extraction according to claim 1, characterized in that: The arc-shaped plate slides in the guide groove, which is opened in the support plate. A toothed plate is fixedly connected to one side of the arc-shaped plate. The toothed plate meshes with a second gear. The second gear is fixedly connected to the output end of the connecting motor. The connecting motor is fixedly connected in the support plate.

4. The separation device for separating polysaccharides from perilla using aqueous two-phase extraction according to claim 1, characterized in that: The stirring mechanism includes a connecting shaft, which is movably connected to the separation mixing tube. The lower end of the connecting shaft extends out of the separation mixing tube and is fixedly connected to a connecting gear. The connecting gear meshes with a second internal gear ring. A T-shaped stirring rod is movably connected to the upper end of the connecting shaft.

5. The separation device for separating polysaccharides from perilla using aqueous two-phase extraction according to claim 4, characterized in that: The T-shaped stirring rod is movably connected to the connecting cavity, which is located at the upper end of the connecting shaft. A first spring is fixedly connected inside the connecting cavity, and the other end of the first spring is fixedly connected to the T-shaped stirring rod.

6. A separation method, used in the separation apparatus for separating polysaccharides from perilla using aqueous two-phase extraction as described in any one of claims 1-5, characterized in that, include: S1. By storing water, organic solvent, and crushed perilla liquid in three sets of storage tanks, open the first solenoid valves of the three sets. At this time, water, organic solution, and perilla will enter the corresponding connecting tanks in sequence. After the connecting tanks are filled, close the first solenoid valves, start the drive motor to move the separation mixing tube to the lower side of the telescopic cylinder, open the telescopic cylinder to allow the liquid adding tube to enter the liquid adding through hole, and put water, organic solution, and crushed perilla into the separation mixing tube. After the liquid adding tube returns to its original position, turn on the drive motor to make the separation mixing tube rotate. S2. When the connecting ring rotates, the stirring mechanism will start to rotate to stir the material in the separation mixing tube. After the mixing and separation are completed, the connecting ring stops rotating, and the hydraulic cylinder will drive the support plate to move upward, so that the first sealing cover will be separated from the upper end of the separation mixing tube. By adjusting the arc plate, the second sealing cover will be moved to the upper end of the separation mixing tube. The hydraulic cylinder will drive the support plate to move downward, and the connecting cover will enter the separation mixing tube to filter the perilla residue between the upper and lower phase liquids into the connecting cover and push it to the bottom of the separation mixing tube. S3. By connecting the two sets of liquid guide tubes and the liquid drain tube to external equipment, the perilla residue is extracted from the separation and mixing tube through the liquid drain tube. The liquid in the lower phase is extracted first through one set of liquid guide tubes. After the lower phase liquid is extracted, the remaining upper phase liquid is extracted. After all the liquid and perilla residue are extracted from the separation and mixing tube, the first sealing cap is re-clamped on the top of the separation and mixing tube, and water, organic solution and perilla liquid are introduced into the tube again. The extracted upper and lower phase liquids are evaporated and dried to obtain the extract.

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