Use of a wind-protecting essential oil in the manufacture of a product for the treatment and prevention of acne

Abstract

The application discloses application of a wind-preventing essential oil in preparation of a product for treating and preventing acne, and the essential oil extracted from the Chinese herbal medicine wind-preventing can prevent and treat acne through anti-inflammatory and anti-oxidation, and the application verifies the relieving effect of the wind-preventing essential oil on the mouse ear redness and swelling, inflammation factor gathering and intracellular active oxygen release of the acne-like mouse ear through a mouse ear acne model induced by propionibacterium acnes. The wind-preventing essential oil shows an inhibiting effect on the activation of NLRP3 inflammasome on the mouse ear skin acne model induced by propionibacterium acnes, and significantly reduces the expression of IL-1beta, IL-6 and TNF-alpha inflammation factors in the mouse tissue. It is suggested that the wind-preventing essential oil has remarkable activity in anti-inflammatory and anti-oxidation and acne-removing, and has a good prospect in preparation of a product for treating and preventing inflammatory acne.

Description

Technical Field

[0001] This invention relates to the application of a buspirant essential oil in the preparation of products for treating and preventing acne. Background Technology

[0002] Acne is a common chronic inflammatory skin disease caused by abnormal sebaceous gland secretion, manifesting as lesions such as pustules, pimples, papules, and cysts, typically occurring on the face and chest. Acne vulgaris has become a common skin condition among teenagers and young adults, as 80% to 90% of adolescents suffer from moderate to severe acne vulgaris, which can persist into adulthood. While acne is often considered a cosmetic issue, the disease can lead to permanent scarring and even disfigurement, and is also associated with various psychological problems such as depression, anxiety, frustration, and social isolation. Propionibacterium acnes is a closely related pathogen. Literature reports that Propionibacterium acnes infection can induce the production of reactive oxygen species (ROS) and activate a series of inflammatory responses, activating the NLRP3 inflammasome and thus increasing the expression of inflammatory factors such as interleukin (IL)-1β. From inflammatory herpes and pustules to post-inflammatory erythema and post-inflammatory hyperpigmentation, the inflammatory response persists throughout the pathogenesis of acne; this is the main pathological feature of acne and a major concern for patients. Currently, hormonal and retinoid drugs are commonly used in clinical practice to treat acne. These drugs have relatively ideal therapeutic effects, but long-term use can easily produce some toxic side effects. Antibacterial drugs, after killing pathogens, may leave residual bacteria and antigenic proteins that can still irritate the face and cause inflammation. Steroidal anti-inflammatory drugs have good anti-inflammatory activity, but they are prone to causing pigmentation and are not accepted by patients. Essential oils extracted from the traditional Chinese medicine plant Saposhnikovia divaricata have significant anti-inflammatory effects. Due to their low toxicity, high safety, good permeability, and the low cost and easy availability of raw materials, they have a huge market potential.

[0003] Microbial infection, endogenous danger signals, and environmental stimuli are all associated with the NLRP3 inflammasome, a complex composed of NOD-like receptor protein 3 (NLRP3), apoptosis-associated speckle protein (ASC), and pro-caspase-1. Activation of the NLRP3 inflammasome is manifested by increased expression of Caspase-1 and IL-1β.

[0004] Saposhnikovia divaricata is a perennial herb widely distributed in eastern Siberia and northern Asia. Its dried root is a traditional Chinese medicine used to treat diseases of the immune and respiratory systems. Essential oil extracted from the root via steam distillation has been reported to inhibit the production of the IL-1β inflammatory cytokine. However, whether it regulates the NLRP3 inflammasome-mediated IL-1β production process remains unclear. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide an application of Saposhnikovia divaricata essential oil in the preparation of products for treating and preventing acne. Saposhnikovia divaricata essential oil has significant antioxidant activity and improves skin inflammation, thereby improving acne. It can be used to prepare functional cosmetics such as acne removal and repair products, as well as traditional Chinese medicine gels.

[0006] The technical solution to achieve the above objectives is: the application of a wind-spreading essential oil in the preparation of products for treating and preventing acne.

[0007] The aforementioned application of Saposhnikovia divaricata essential oil, wherein the essential oil is obtained by extracting the Chinese herbal medicine Saposhnikovia divaricata by steam distillation after pulverizing it.

[0008] The aforementioned application of the windproof essential oil includes the fact that the essential oil inhibits the generation of ROS caused by Propionibacterium acnes in vivo and has antioxidant activity.

[0009] The aforementioned application of the anti-inflammatory essential oil involves the essential oil inhibiting the expression of IL-1β, IL-6 and TNF-α inflammatory factors in vivo and preventing the activation of the NLRP3 inflammasome caused by Propionibacterium acnes, thus exhibiting anti-inflammatory activity and inhibiting inflammasome activation.

[0010] In the above-mentioned application of the windproof essential oil, the inhibition of inflammasome activation includes inhibiting the inflammasome-induced cleavage of Caspase-1 protein in mice, inhibiting the inflammasome-induced maturation of IL-1β in mice, inhibiting the inflammasome-induced secretion of IL-1β in mice, and inhibiting the inflammasome-induced formation of GSDMD-NT in mice.

[0011] The aforementioned applications of windproof essential oils include pharmaceuticals and daily chemical products.

[0012] In the above-mentioned application of the windproof essential oil, the essential oil has a weight content of 0.1%-1% in the product.

[0013] In the above-mentioned application of the windproof essential oil, the dosage form of the medicine is at least one of capsules, pellets, injections, aerosol powders, films, patches, emulsions, creams, liniments, lotions, and lotions.

[0014] In the above-mentioned application of windproof essential oil, the daily chemical product is at least one of shampoo, hair cream, shower gel, body lotion, essential oil soap, mentholatum, medicated oil, toner, floral water, face cream, face mask and facial essence.

[0015] The present invention relates to the application of Saposhnikovia divaricata essential oil in the preparation of products for the treatment and prevention of acne. Saposhnikovia divaricata essential oil has significant antioxidant activity and improves skin inflammation, thereby improving acne. After preparing a mouse acne model induced by Propionibacterium acnes induced by ultrasonic disruption, hydrogels of Saposhnikovia divaricata essential oil extract in different proportions were administered. By measuring the degree of skin swelling in mice and the expression of macrophages and inflammatory factors in the skin, it was confirmed that Saposhnikovia divaricata essential oil has the effect of improving the mouse acne model induced by Propionibacterium acnes. This indicates that Saposhnikovia divaricata essential oil can be used to prevent and treat inflammatory acne and has broad application prospects. Attached Figure Description

[0016] Figure 1 Total ion chromatogram for GC-MS analysis of Saposhnikovia divaricata essential oil;

[0017] Figure 2 To investigate the toxic effects of Fangfeng essential oil on J774A.1 macrophages;

[0018] Figure 3 The effect of Saposhnikovia divaricata essential oil on the expression levels of inflammatory factors in J774A.1 cells induced by Propionibacterium acnes;

[0019] Figure 4 The effect of Fangfeng essential oil on ear redness and swelling in mice with acne induced by Propionibacterium acnes;

[0020] Figure 5 The effect of Saposhnikovia divaricata essential oil on ear thickness in mice with acne induced by Propionibacterium acnes;

[0021] Figure 6 HE staining comparison of ear skin in mice with acne induced by Propionibacterium acnes using Fangfeng essential oil;

[0022] Figure 7 Comparative images of DHE staining of ear skin in mice with acne induced by Propionibacterium acnes using Fangfeng essential oil;

[0023] Figure 8 The effect of Saposhnikovia divaricata essential oil on macrophages in the ear skin of mice with acne induced by Propionibacterium acnes;

[0024] Figure 9 The effect of Saposhnikovia divaricata essential oil on the expression levels of inflammatory factors in the skin tissue fluid of mice with acne induced by Propionibacterium acnes (A is TNF-α inflammatory factor, B is IL-1β inflammatory factor, and C is IL-6 inflammatory factor);

[0025] Figure 10 Western blotting analysis was performed on proteins in the ear skin of mice with acne induced by Propionibacterium acnes to protect against wind. Detailed Implementation

[0026] To enable those skilled in the art to better understand the technical solution of the present invention, its specific embodiments are described in detail below with reference to the accompanying drawings:

[0027] Example 1: Identification of the chemical composition of Saposhnikovia divaricata essential oil

[0028] (1) Preparation of test sample

[0029] Take 700±5 mg of Saposhnikovia divaricata essential oil into a 20 mL headspace vial, and add 10 μL of 2-octanol (10 mg / L stock indH2O) as an internal standard.

[0030] (2) GC-MS conditions

[0031] In the SPME cycle of the PAL orbital system, the extraction temperature was 60℃, the preheating time was 15 min, the extraction time was 30 min, and the elution time was 4 min. This experiment used an Agilent 7890 gas chromatograph system, a 5977B mass spectrometer, and a DB-Wax column (30m × 250μm × 0.25μm). Helium was used as the carrier gas, and the septum purge flow rate was 3 mL / min. -1 The gas flow rate through the column is 1 mL / min. -1 The initial temperature was maintained at 40°C for 4 minutes, then increased at 5°C for 5 minutes. -1 The ionization rate was increased to 245℃ and held for 5 min. The inlet temperature, transfer line temperature, ion source temperature, and quadrupole temperature were 250℃, 250℃, 230℃, and 150℃, respectively. The ionization voltage in electron impact mode was -70 eV. Mass spectrometry data were obtained in scan mode, with a mass range of 20-400 and a solvent delay range of 0 min. The GC-MS total ion chromatogram of the *Saposhnikovia divaricata* essential oil is shown below. Figure 1 As shown in Table 1, the identification results of the main chemical components of the Saposhnikovia divaricata essential oil are as follows.

[0032] Table 1. Chemical composition of Saposhnikovia divaricata essential oil:

[0033]

[0034]

[0035] Example 2: Evaluation of the cytotoxicity of Saposhnikovia divaricata essential oil against J774A.1 macrophages

[0036] Please see Figure 2 J774A.1 cells were cultured in DMEM medium containing 8% fetal bovine serum at a rate of 5 × 10⁻⁶. 3Cells were seeded into 96-well plates for 16 hours. Then, 100 μg / mL of Saposhnikovia divaricata oil emulsion was serially diluted two-fold to a final concentration of 3.125 μg / mL, resulting in six concentrations. Each concentration was used in triplicate. DMEM medium containing 8% fetal bovine serum was used as a control, and the plates were incubated in a 5% CO2 incubator for 24 hours. 20 μL of MTT solution was added to each well, and the plates were incubated for 4 hours. After discarding the supernatant, 200 μL of DMSO was added, and the plates were shaken for 5 minutes. The absorbance at 450 nm was then measured using a BioTek 800TS microplate reader.

[0037] The results are as follows Figure 2 As shown, concentrations of 50 μg / mL and below had no significant effect on the viability of J774A.1 cells, indicating that the essential oil of Saposhnikovia divaricata is safe within this concentration range.

[0038] Example 3: Effect of Saposhnikovia divaricata essential oil on the expression level of IL-1β inflammatory factor in J774A.1 cells induced by Propionibacterium acnes

[0039] (1) Sample preparation: J774A.1 cells were cultured in DMEM medium containing 8% fetal bovine serum at a concentration of 1×10⁻⁶. 6 Cells were seeded into 6-well plates. On the second day, 200 ng / mL LPS was added to each well and incubated for 3 hours. The supernatant was discarded, and 30 μg / mL, 10 μg / mL, and 3 μg / mL of Saposhnikovia divaricata essential oil emulsion were added to each well and incubated for 30 minutes. Propionibacterium acnes with an MOI of 100 was then added, and the plates were incubated overnight. The supernatant was used as the sample. The sample can be used immediately or stored at -80°C for later use.

[0040] (2) ELISA: Follow the instructions in the manual. Finally, place the ELISA plate in the microplate reader and read the absorbance value at 450 nm using 630 nm as the calibration wavelength. Calculate the IL-1β concentration in the sample based on the standard curve.

[0041] The results are as follows Figure 3 As shown, Saposhnikovia divaricata essential oil can reduce the expression level of IL-1β in J774A.1 cells induced by Propionibacterium acnes in a dose-dependent manner.

[0042] Example 4: Effect of Saposhnikovia divaricata essential oil on erythema and swelling induced by Propionibacterium acnes in ICR mice

[0043] In an SPF-grade breeding environment, 6-week-old male ICR mice were used for the experiment; Modeling method: (1) After stabilizing the 5-week-old male ICR mice (approximately 25g each) for one week, each mouse was injected with 20μL of a drug at a density of 1×10 9CFU / ml of Propionibacterium acnes. Six treatments were set up, with six biological replicates for each treatment, totaling 36 mice. The drug was applied immediately after modeling, and a second dose was administered 12 hours later. Mice were sacrificed 24 hours later. The treatment methods are as follows:

[0044] Treatment 1 (blank group): Mice received no treatment.

[0045] Treatment 2 (model group): Propionibacterium acnes was injected into the ear to create an acne model group.

[0046] Treatment 3 (andrographolide group): From the time of Propionibacterium acnes injection to establish the model, 0.5% andrographolide was applied topically to the ear, which was the positive drug group.

[0047] Treatment 4 (low-dose administration group): From the time of Propionibacterium acnes injection to establish the acne model, 0.25% Saposhnikovia divaricata essential oil was applied topically to the ear, which was the low-dose administration group.

[0048] Treatment 5 (medium-dose administration group): From the time of Propionibacterium acnes injection to establish the acne model, 0.5% Saposhnikovia divaricata essential oil was applied topically to the ear, which was the medium-dose administration group;

[0049] Treatment 6 (high-dose administration group): From the time of Propionibacterium acnes injection to establish the acne model, the ear was given topical application of 1% Saposhnikovia divaricata essential oil, which is the high-dose administration group.

[0050] Other routine management procedures remained consistent across the six treatments. Please refer to [link / reference]. Figure 4 , Figure 5 and Figure 6 After the experiment, tissue samples were collected from the sacrificed mice, and the redness and swelling of the mouse ears and the pathological HE staining were analyzed. Figure 4 As shown, compared with the control group, the Propionibacterium acnes model mice exhibited ear redness and swelling. Compared with the model mice, the high, medium, and low doses of Saposhnikovia divaricata essential oil all improved ear redness and swelling, with the high dose group showing a more significant alleviating effect. Andrographolide in the positive control group showed a very significant therapeutic effect. HE staining analysis of the mouse ears is shown below. Figure 6 As shown, compared with the control group, the sebaceous gland diameter of the Propionibacterium acnes model mice was significantly increased, and inflammatory cell infiltration was severe. Compared with the model group, high, medium, and low doses of Saposhnikovia divaricata essential oil treatment groups all improved the enlarged sebaceous gland diameter and inflammatory cell infiltration in the mouse ears, with the high-dose treatment group showing a more significant effect. Ear thickness was measured at each section location under a 50x microscope. Ear thickness was measured in six mice per group, with three measurements taken for each mouse and the average value recorded. Statistical results are shown below. Figure 5As shown, compared with the control group, the model group exhibited pustules and increased ear thickness. Compared with the model group, all treatment groups with high, medium, and low doses of Saposhnikovia divaricata essential oil improved the pustules caused by Propionibacterium acnes, resulting in reduced ear thickness. The positive control drug, Andrographolide, showed significant effects, with ear thickness approaching that of the control group. Therefore, Saposhnikovia divaricata essential oil has a certain alleviating effect on ear redness and pustules induced by Propionibacterium acnes in mice, and may potentially become a safe and low-toxicity new drug for acne.

[0051] Example 5: Effects of Saposhnikovia divaricata essential oil on reactive oxygen species in the ear skin of mice with acne induced by Propionibacterium acnes

[0052] Studies have reported that acne models generate large amounts of reactive oxygen species. To verify whether the essential oil of *Saposhnikovia divaricata* exerts its medicinal effect through antioxidant activity, DHE (Dihydroethidium) staining was performed on mouse ear tissue, as follows:

[0053] (1) Quenching of autofluorescence in tissue: Frozen sections were rewarmed at room temperature and dried. Circles were drawn around the tissue with a histochemical pen, autofluorescence quencher was added for 5 min, and the tissue was rinsed with running water for 10 min.

[0054] (2) Staining: Add ROS staining solution to the circle and incubate at 37°C for 30 min in a light-proof constant temperature incubator.

[0055] (3) DAPI counterstaining of cell nuclei: Place the slide in PBS (pH 7.4) and wash three times on a decolorizing shaker for 5 min each time. Add DAPI staining solution and incubate at room temperature in the dark for 10 min.

[0056] (4) Mounting: Place the slide in PBS (pH 7.4) and wash it three times on a decolorizing shaker for 5 minutes each time. Mount the slide with anti-fluorescence quenching mounting medium.

[0057] (5) Image acquisition: DAPI excitation wavelength 330-380nm, emission wavelength 420nm; 488 excitation wavelength 465-495nm, emission wavelength 515-555nm; CY3 excitation wavelength 510-560nm, emission wavelength 590nm; CY5 excitation wavelength 608-648nm, emission wavelength 672-712nm.

[0058] The results are as follows Figure 7 As shown, DAPI channel nuclei are blue, while CY3 channel positivity is red. In the model group, *Propionibacterium acnes* induced the production of large amounts of reactive oxygen species in mouse tissues, resulting in intense red light. The treatment group showed significant improvement in a dose-dependent manner.

[0059] Example 6: Effects of Saposhnikovia divaricata essential oil on macrophages in the ear skin of mice with acne induced by Propionibacterium acnes

[0060] To investigate the relationship between acne models and inflammation, F4 / 80 immunohistochemical experiments were performed on mouse tissues.

[0061] The experimental steps are as follows:

[0062] (1) Dewaxing paraffin sections to water: The sections were placed in environmentally friendly dewaxing solution I for 10 min, environmentally friendly dewaxing solution II for 10 min, environmentally friendly dewaxing solution III for 10 min, anhydrous ethanol I for 5 min, anhydrous ethanol II for 5 min, anhydrous ethanol III for 5 min, and then washed with distilled water.

[0063] (2) Antigen retrieval: See the table above for retrieval instructions. During this process, excessive evaporation of the buffer solution should be prevented, and the slides should not be dried out. After natural cooling, place the slides in PBS (pH 7.4) and wash them three times on a decolorizing shaker for 5 minutes each time. (The retrieval solution and conditions should be determined based on the tissue.)

[0064] (3) Blocking endogenous peroxidase: Place the slide in 3% hydrogen peroxide solution and incubate at room temperature in the dark for 25 min. Then place the slide in PBS (pH 7.4) and wash it three times on a decolorizing shaker for 5 min each time.

[0065] (4) Serum blocking: Add 3% BSA evenly to the histochemistry zone and block at room temperature for 30 min.

[0066] (5) Add primary antibody: Gently shake off the blocking solution, add the primary antibody prepared in PBS at a certain ratio to the slide, and incubate the slide in a humidified chamber at 4°C overnight.

[0067] (6) Add secondary antibody: Place the slide in PBS (pH 7.4) and wash it three times on a decolorizing shaker for 5 minutes each time. After slightly drying the slide, add the secondary antibody (HRP-labeled) of the corresponding species to the primary antibody to cover the tissue and incubate at room temperature for 50 minutes.

[0068] (7) DAB staining: Place the slide in PBS (pH 7.4) and wash it three times on a decolorizing shaker for 5 minutes each time. After slightly drying the slide, add freshly prepared DAB staining solution to the circle. Control the staining time under a microscope. The positive result is brownish-yellow. Rinse the slide with tap water to stop the staining.

[0069] (8) Counterstaining cell nuclei: Counterstain with hematoxylin for about 3 minutes, wash with tap water, differentiate with hematoxylin differentiation solution for a few seconds, rinse with tap water, re-blue with hematoxylin blue solution, and rinse with running water.

[0070] (9) Dehydration and mounting: Place the sections in 75% alcohol for 5 min, 85% alcohol for 5 min, anhydrous ethanol I for 5 min, anhydrous ethanol II for 5 min, n-butanol for 5 min, and xylene I for 5 min in sequence to dehydrate and make them transparent. Remove the sections from the xylene and let them dry slightly. Then mount them with mounting medium.

[0071] (10) Microscopic examination: The results are interpreted under a white light microscope.

[0072] The results are as follows Figure 8 As shown, hematoxylin stains cell nuclei blue, while DAB shows positive expression as brownish-yellow. Compared to the control group, the model group showed an increase in macrophages, with a large number of macrophages clustering at the model site. Compared to the model group, the group treated with Saposhnikovia divaricata essential oil and Andrographolide significantly improved this condition.

[0073] Example 7: Effect of Saposhnikovia divaricata essential oil on the expression levels of inflammatory factors in the skin tissue fluid of the ear of mice with acne induced by Propionibacterium acnes.

[0074] Sample preparation: Ear tissue from sacrificed mice was cryogenically ground in liquid nitrogen, then PBS containing protease inhibitors was added, and the mixture was sonicated at 8% power for 2 minutes. After sonication, the tissue was centrifuged at 4°C (8000 rpm, 10 minutes). The supernatant was collected as the sample and stored at -80°C for later use.

[0075] ELISA: Follow the instructions in the manual. Finally, place the ELISA plate in the microplate reader, calculate the IL-1β concentration in the sample based on the standard curve, and then place the ELISA plate in the microplate reader to detect the IL-1β, IL-6, and TNF-α at the corresponding detection wavelengths and calibration wavelengths.

[0076] The results are as follows Figure 9 As shown, *Propionibacterium acnes* can induce the production of inflammatory factors IL-1β, IL-6, and TNF-α in mice. Saposhnikovia divaricata essential oil can dose-dependently reduce the expression levels of inflammatory factors induced by *Propionibacterium acnes* in mice, with andrographolide showing a more significant effect in reducing inflammatory factors.

[0077] Example 8: Western blotting analysis of proteins in the ear skin of mice with acne induced by Propionibacterium acnes using Saposhnikovia divaricata essential oil.

[0078] Ear tissue from sacrificed mice was cryogenically ground in liquid nitrogen, then lysed in an ice-water mixture with RIPA lysis buffer containing a protease inhibitor for 30 min. The tissue was then sonicated at 8% power for 2 min. After sonication, the tissue was centrifuged at 4°C (8000 rpm, 10 min), and the supernatant was used for BCA quantification. The expression of NLRP3 inflammasome-related protein in mouse ear tissue was measured using Western blot.

[0079] The results are as follows Figure 10As shown, compared with the normal group, pro-IL-1β, pro-Caspase-1, and GSDMD-FL were all cleaved in the model group to generate medium IL-1β, Caspase-1, and GSDMD-NT, indicating that the acne model activated the NLRP3 inflammasome. After treatment with Saposhnikovia divaricata essential oil in mice after modeling, IL-1β, Caspase-1, and GSDMD-NT proteins were downregulated, indicating that Saposhnikovia divaricata essential oil can inhibit the activation of the NLRP3 inflammasome.

[0080] In summary, the application of the Saposhnikovia divaricata essential oil in the preparation of products for treating and preventing acne demonstrates that it can inhibit the release of IL-1β inflammatory factors induced by Propionibacterium acnes in J774A.1 cells. The effect of Saposhnikovia divaricata essential oil on alleviating ear redness and swelling, inflammatory factor accumulation, and intracellular reactive oxygen species release in acne-like mice was verified using a Propionibacterium acnes-induced mouse ear acne model. Therefore, it has promising application prospects in the preparation of products for treating and preventing acne.

[0081] Those skilled in the art should recognize that the above embodiments are merely illustrative of the present invention and are not intended to limit the present invention. Any variations or modifications to the above embodiments that are within the spirit and essence of the present invention will fall within the scope of the claims of the present invention.

Claims

1. The application of a Saposhnikovia divaricata essential oil in the preparation of products for treating and preventing acne, characterized in that, The essential oil inhibits the generation of ROS caused by Propionibacterium acnes in vivo and has antioxidant activity. The essential oil inhibits the expression of inflammatory factors IL-1β, IL-6 and TNF-α in vivo and prevents the activation of the NLRP3 inflammasome caused by Propionibacterium acnes, thus exhibiting anti-inflammatory activity and inhibiting inflammasome activation.

2. The application of the wind-repelling essential oil according to claim 1, characterized in that, The essential oil is extracted by steam distillation after the Chinese herbal medicine Saposhnikovia divaricata is pulverized.

3. The application of the wind-repelling essential oil according to claim 1, characterized in that, The inhibition of inflammasome activation includes inhibiting inflammasome-induced Caspase-1 protein cleavage in mice, inhibiting inflammasome-induced IL-1β maturation in mice, inhibiting inflammasome-induced IL-1β secretion in mice, and inhibiting inflammasome-induced GSDMD-NT formation in mice.

4. The application of the wind-repelling essential oil according to claim 1, characterized in that, The product in question is a pharmaceutical or daily chemical product.

5. The application of the wind-repelling essential oil according to claim 4, characterized in that, The essential oil has a weight content of 0.1%-1% in the product.

6. The application of the wind-repelling essential oil according to claim 5, characterized in that, The dosage form of the drug is at least one of the following: capsules, pellets, injections, aerosols, powders, films, patches, emulsions, creams, liniments, and lotions.

7. The application of the wind-repelling essential oil according to claim 5, characterized in that, The daily chemical products mentioned are at least one of the following: shampoo, hair cream, shower gel, body lotion, essential oil soap, toner, face cream, face mask, and facial serum.

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