A biological smoke paste and a preparation method and application thereof

Abstract

This invention discloses a bio-tobacco paste and its preparation method. The bio-tobacco paste is prepared by fermenting tobacco using a mixture of *Klebsiella variicola* HNYJ-3 (CGMCC No. 29654) and *Enterobacter sp.* HNYJ-4 (CGMCC No. 29655). Through a specific microbial fermentation process, the bio-tobacco paste significantly increases the low-boiling-point aroma components in tobacco, improving the aroma quality and release efficiency of heated cigarettes. Heated cigarettes with the added bio-tobacco paste exhibit superior aroma, balance, and aftertaste in sensory evaluations, while reducing off-flavors and irritation, thereby improving the overall sensory experience of smoking.

Description

Technical Field

[0001] This invention relates to the field of microbial technology, and in particular to a bio-tobacco paste, its preparation method, and its application. Background Technology

[0002] Heated cigarettes are a relatively new type of tobacco product. They use an electronic heating device to heat a specially designed cigarette, releasing aroma components and nicotine into the smoke to satisfy the smoker's cravings. Traditional cigarettes burn at temperatures reaching over 1000 degrees Celsius, allowing for the complete transfer and breakdown of aroma and harmful substances in the tobacco. In contrast, heated cigarettes are typically heated to between 200-300 degrees Celsius. While less harmful substances are transferred, most of the aroma components in the tobacco also fail to transfer or break down into the smoke. Therefore, the aroma of heated cigarettes can never match that of traditional cigarettes.

[0003] There are limited methods to improve the aroma of heated cigarettes, mainly including optimizing the heating method, improving the tobacco leaf formula, and optimizing the flavoring formula. Ultimately, it requires releasing as many aroma components as possible from the heated cigarette at temperatures below 250℃. Considering the loss due to flavoring volatilization, aroma components with boiling points between 100-250℃ are generally preferred. Flavoring is a crucial component of heated cigarettes, determining to a certain extent the types and amounts of aroma released. In particular, the tobacco paste, used in the largest quantity, is the most important flavoring agent in heated cigarettes, providing the most essential natural tobacco aroma. Therefore, developing tobacco paste specifically for heated cigarettes is crucial for improving the quality of heated cigarettes.

[0004] Currently, the optimization of flavor and fragrance formulations for heated cigarettes mainly involves increasing the amount used, but the effect is limited. There are also traditional physicochemical methods to lock in flavor substances with low boiling points as much as possible to prepare special tobacco paste for heated cigarettes, but the cost-effectiveness is low and the cost is relatively high.

[0005] This invention utilizes mixed-culture fermentation of tobacco to enrich aroma substances with boiling points below 250°C, such as nocaketone, β-ionone, and 2-acetylpyrrole, thereby obtaining a bio-tobacco paste specifically for heated cigarettes. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to provide a biological tobacco paste and its preparation method and application, which solves the problem of insufficient aroma release of heated cigarettes compared with traditional cigarettes and enhances the release of aroma components.

[0007] The technical problem to be solved by the present invention is achieved through the following technical solution:

[0008] A bio-tobacco paste, wherein the bio-tobacco paste is prepared by fermenting tobacco with a mixture of Klebsiella variicola HNYJ-3 (CGMCC No. 29654) and Enterobacter p. HNYJ-4 (CGMCC No. 29655).

[0009] A method for preparing a bio-tobacco paste includes the following steps:

[0010] (1) Preparation of flue-cured tobacco water extract, burley tobacco water extract and aromatic tobacco water extract: The middle tobacco leaves of flue-cured tobacco, burley tobacco and aromatic tobacco are crushed and passed through a 50-mesh sieve to obtain tobacco powder; the tobacco powder is then mixed with pure water to extract the water extracts of flue-cured tobacco, burley tobacco and aromatic tobacco respectively.

[0011] (2) Prepare seed cultures of Klebsiella variegata HNYJ-3 and Enterobacter HNYJ-4 respectively for later use;

[0012] (3) Preparation of mixed slurry: The flue-cured tobacco water extract, burley tobacco water extract and spice tobacco water extract from step (1) are mixed in a volume ratio of (5-10):(1-5):(1-5), and the mixture is then mixed with coffee chlorogenic acid in a volume ratio of 1:10-1:100 to obtain mixed slurry.

[0013] (4) Preparation of fermentation mixed slurry: The Klebsiella variegata HNYJ-3 seed liquid prepared in step (2) is mixed with Enterobacter HNYJ-4 seed liquid in a volume ratio of 1:10-10:1 to obtain a composite seed liquid.

[0014] (5) Mix the composite seed liquid from step (4) with the mixed slurry from step (2) at a volume ratio of 1:1000-1:100, and ferment at a constant temperature of 25-40℃ for 12-96 hours with shaking at a speed of 180 rpm. Centrifuge and collect the supernatant to obtain the fermented mixed slurry. Concentrate the fermented mixed slurry under reduced pressure at 60℃ to 1 / 10 of the original volume to obtain the concentrated slurry.

[0015] (6) The concentrated slurry is mixed with anhydrous ethanol at a mass ratio of 2:1-1:10, and centrifuged to obtain crude biological tobacco paste. The crude biological tobacco paste is concentrated under reduced pressure to obtain biological tobacco paste.

[0016] Preferably, the preparation conditions for the aqueous extract in step (1) are as follows: flue-cured tobacco powder, burley tobacco powder, and aromatic tobacco powder are mixed with pure water at a ratio of 1:20 to 1:5, extracted at a constant temperature of 30-100℃ for 1-10 hours, and filtered through 200-mesh gauze to obtain the three aqueous extracts.

[0017] Preferably, the seed culture preparation in step (2) involves inoculating Klebsiella variegata HNYJ-3 and Enterobacter hNYJ-4 into LB liquid medium, respectively, and culturing at 30°C for 24 hours, OD... 600 A pH of 1.0-3.0 indicates that it is seed liquid.

[0018] Preferably, step (3) includes:

[0019] The flue-cured tobacco aqueous extract, burley tobacco aqueous extract and spice tobacco aqueous extract prepared in step (1) are mixed in a volume ratio of 7:2:1 to obtain a mixed tobacco aqueous extract.

[0020] Tobacco aqueous extract and coffee chlorogenic acid were mixed at a volume-to-mass ratio of 1:50 to obtain a mixed slurry.

[0021] Preferably, step (4) specifically involves mixing the Klebsiella variegata HNYJ-3 seed solution prepared in step (2) with the Enterobacter HNYJ-4 seed solution at a volume ratio of 2:1 to obtain a composite seed solution.

[0022] Preferably, in step (5), the composite seed liquid and the mixed slurry are mixed at a volume ratio of 1:200 and fermented at a constant temperature of 30°C for 48 hours.

[0023] Preferably, step (6) specifically involves: mixing the concentrated slurry with anhydrous ethanol at a mass ratio of 1:2, letting it stand at -20°C for 24 hours, centrifuging to obtain crude biological tobacco paste, and concentrating the crude biological tobacco paste under reduced pressure at 60°C to 1 / 5 of its original volume to obtain biological tobacco paste.

[0024] The application of a bio-tobacco paste in heated cigarettes, wherein the bio-tobacco paste is used to enhance the aroma quality and sensory experience of heated cigarettes.

[0025] A method for improving the sensory quality of heated cigarettes, the method being to add bio-tobacco paste to heated cigarettes to improve their aroma, harmony, reduce off-flavors, reduce irritation and enhance aftertaste.

[0026] The above-described technical solution of the present invention has the following beneficial effects:

[0027] (1) Enhanced aroma components: Bio-tobacco paste significantly increases the low-boiling-point aroma components in tobacco through a specific microbial fermentation process, thereby improving the aroma quality and release efficiency of heated cigarettes.

[0028] (2) Improved sensory experience: Heated cigarettes with added bio-tobacco paste showed better aroma, harmony and aftertaste in sensory evaluation, and reduced off-flavors and irritation, thereby improving the overall sensory experience of smoking.

[0029] (3) Innovation in preparation process: By using the mixed fermentation of Klebsiella variegata HNYJ-3 and Enterobacter HNYJ-4, a cost-effective method for preparing bio-tobacco paste was developed, providing a new technical path for improving the quality of heated cigarettes. Detailed Implementation

[0030] Various exemplary embodiments of the present invention will now be described in detail. It should be noted that, unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of the components and steps set forth in these embodiments do not limit the scope of the invention.

[0031] A bio-tobacco paste, its preparation method, and its application are disclosed. The bio-tobacco paste is obtained by fermenting tobacco with a mixture of Klebsiella variicola HNYJ-3 and Enterobacter sp. HNYJ-4.

[0032] Klebsiella variicola HNYJ-3, derived from the surface of tobacco leaves, is taxonomically named Klebsiella variicola HNYJ-3 and is deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. The accession number is CGMCC No. 29654, and the deposit date is January 18, 2024.

[0033] Enterobacter sp. HNYJ-4, derived from the surface of tobacco leaves, is taxonomically named Enterobacters sp. HNYJ-4 and is deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. The accession number is CGMCC No. 29655, and the deposit date is January 18, 2024.

[0034] Example 1: Preparation method of bio-tobacco paste

[0035] Biological tobacco paste and its preparation method include the following steps:

[0036] (1) Preparation methods of flue-cured tobacco aqueous extract, burley tobacco aqueous extract and spice tobacco aqueous extract:

[0037] Preparation of flue-cured tobacco aqueous extract: The middle part of the flue-cured tobacco leaves is crushed and passed through a 50-mesh sieve to obtain flue-cured tobacco powder. The flue-cured tobacco powder is mixed with pure water at a ratio of 1:20-1:5, and extracted at a constant temperature of 30-100℃ for 1-10 hours. The extract is then filtered through 200-mesh gauze to obtain the flue-cured tobacco aqueous extract.

[0038] Preparation of Burley tobacco aqueous extract: The middle part of the Burley tobacco is crushed, and the other steps are the same as those for the preparation of tobacco aqueous extract.

[0039] Preparation of aromatic tobacco aqueous extract: The middle part of the aromatic tobacco leaves is crushed, and the other steps are the same as those for the preparation of tobacco aqueous extract.

[0040] (2) Preparation of strain seed liquid:

[0041] Klebsiella variegata HNYJ-3 and Enterobacter HNYJ-4 were inoculated into LB liquid medium and incubated at 30°C for 24 h. OD 600 When the concentration reaches around 1.0-3.0, it is considered seed liquid.

[0042] LB liquid medium is a standard formulation and will not be described in detail here.

[0043] (3) Preparation of mixed slurry:

[0044] The flue-cured tobacco aqueous extract, burley tobacco aqueous extract and aromatic tobacco aqueous extract prepared in Example 1 were mixed in a volume ratio of (5-10):(1-5):(1-5) to obtain a mixed tobacco aqueous extract;

[0045] A mixed tobacco aqueous extract was mixed with caffeine chlorogenic acid (25% purity) at a volume-to-mass ratio of 1:10 to 1:100 to obtain a mixed slurry.

[0046] (4) Preparation of fermentation mixed slurry:

[0047] A composite seed solution was obtained by mixing Klebsiella variegata HNYJ-3 seed solution and Enterobacter cytomegalovirus HNYJ-4 seed solution at a volume ratio of 1:10-10:1.

[0048] (5) Compound seed liquid and mixed slurry:

[0049] Mix the contents at a volume ratio of 1:1000-1:100 and ferment at a constant temperature of 25-40℃ for 12-96 hours with shaking at a speed of 180 rpm. After fermentation, centrifuge and collect the supernatant to obtain the fermented slurry.

[0050] The fermentation mixture was concentrated to 1 / 10 of its original volume under reduced pressure at 60°C to obtain a concentrated slurry.

[0051] The concentrated slurry was mixed with anhydrous ethanol at a mass ratio of 2:1 to 1:10, and allowed to stand at -20℃ for 24 hours. After centrifugation, crude biological tobacco paste was obtained. The crude biological tobacco paste was concentrated under reduced pressure at 60℃ to 1 / 5 of its original volume to obtain biological tobacco paste.

[0052] This tobacco paste contains a large number of low-boiling-point (below 100-250℃) aromatic substances, and has a distinct sweet and floral aroma with a slight ambergris scent, making it suitable for use in heated cigarettes.

[0053] Example 2: Preparation method of bio-tobacco paste

[0054] Biological tobacco paste and its preparation method include the following steps:

[0055] (1) Preparation methods of flue-cured tobacco aqueous extract, burley tobacco aqueous extract and spice tobacco aqueous extract:

[0056] Preparation of flue-cured tobacco aqueous extract: The middle part of the flue-cured tobacco leaves was crushed and passed through a 50-mesh sieve to obtain flue-cured tobacco powder. The flue-cured tobacco powder was mixed with pure water at a ratio of 1:12, and extracted at a constant temperature of 60℃ for 2 hours. The extract was then filtered through 200-mesh gauze to obtain the flue-cured tobacco aqueous extract.

[0057] Preparation of Burley tobacco aqueous extract: The middle part of the Burley tobacco is crushed, and the other steps are the same as those for the preparation of tobacco aqueous extract.

[0058] Preparation of aromatic tobacco aqueous extract: The middle part of the aromatic tobacco leaves is crushed, and the other steps are the same as those for the preparation of tobacco aqueous extract.

[0059] (2) Preparation of strain seed liquid:

[0060] Klebsiella variegata HNYJ-3 and Enterobacter HNYJ-4 were inoculated into LB liquid medium and incubated at 30°C for 24 h. OD 600 When the concentration reaches around 2.0, it is considered seed liquid.

[0061] LB liquid medium is a standard formulation and will not be described in detail here.

[0062] (3) Preparation of mixed slurry:

[0063] The flue-cured tobacco aqueous extract, burley tobacco aqueous extract and aromatic tobacco aqueous extract prepared in Example 1 were mixed in a volume ratio of 7:2:1 to obtain a mixed tobacco aqueous extract.

[0064] A mixed tobacco aqueous extract and caffeic acid (25% purity) were mixed at a volume-to-mass ratio of 1:50 to obtain a mixed slurry.

[0065] (4) Preparation of fermentation mixed slurry:

[0066] A composite seed solution was obtained by mixing Klebsiella variegata HNYJ-3 seed solution and Enterobacter HNYJ-4 seed solution at a volume ratio of 2:1.

[0067] (5) Compound seed liquid and mixed slurry:

[0068] The mixture was prepared by mixing at a volume ratio of 1:200 and fermented at 30℃ with constant temperature shaking for 48 hours at a speed of 180 rpm. After fermentation, the supernatant was collected by centrifugation to obtain the fermented slurry.

[0069] The fermentation mixture was concentrated to 1 / 10 of its original volume under reduced pressure at 60°C to obtain a concentrated slurry.

[0070] The concentrated slurry was mixed with anhydrous ethanol at a mass ratio of 1:2, and allowed to stand at -20℃ for 24 hours. After centrifugation, crude biological tobacco paste was obtained. The crude biological tobacco paste was concentrated under reduced pressure at 60℃ to 1 / 5 of its original volume to obtain biological tobacco paste.

[0071] This tobacco paste contains a large number of low-boiling-point (below 100-250℃) aromatic substances, and has a distinct sweet and floral aroma with a slight ambergris scent, making it suitable for use in heated cigarettes.

[0072] Application Example 1

[0073] (1) Materials:

[0074] The flue-cured tobacco is made from leaves grown in central Kunming, Yunnan in 2022; the burley tobacco is made from leaves grown in central Enshi, Hubei in 2022; and the aromatic tobacco is made from leaves grown in central Baoshan, Yunnan in 2022.

[0075] Coffee chlorogenic acid (25% purity) is commercially available.

[0076] (2) Method:

[0077] Following the method in Example 2, the mixed slurry was fermented separately by Klebsiella variegata HNYJ-3 and Enterobacter HNYJ-4 to prepare bio-tobacco paste, denoted as T1 and T2; the mixed slurry was simultaneously fermented using a compound strain of Klebsiella variegata HNYJ-3 and Enterobacter HNYJ-4 to prepare bio-tobacco paste, denoted as T3; the mixed slurry was not fermented, but the subsequent steps were the same to prepare ordinary tobacco paste, denoted as CK.

[0078] Dichloromethane extraction was used, followed by GC-MS analysis to detect changes in key aroma components in the tobacco paste. The paste was added at a ratio of 100 ppm to Fashida heated cigarettes, and sensory evaluation was conducted by professional judges; the scores were averaged.

[0079] (3) Results:

[0080] The changes in the main aroma components of regular tobacco paste (CK) and bio-tobacco paste are shown in Table 1.

[0081] Compared to regular tobacco paste, the content of key tobacco aroma components in Bio-Tobacco Paste T3 is significantly increased, including ambroxol, 6-methyl-2-heptanone, quinic acid-γ-lactone, and norsodium diketone. These are aroma components that are easily transferred into the smoke during the smoking of heated cigarettes. This Bio-Tobacco Paste has a more pronounced sweet and floral aroma, with a slight ambergris note.

[0082] Compared to T1 and T2, which are fermented by single bacteria, T3 bio-tobacco paste, which is fermented by mixed bacteria, has a higher content of aroma components. It can also be seen that ambroxol is mainly produced by the fermentation of Enterobacter HNYJ-4, and 6-methyl-2-heptanone is mainly produced by the fermentation of Klebsiella variegata HNYJ-3. Both bacteria can produce quinic acid-γ-lactone and solanidine. Only the combined fermentation of the two bacteria can produce the best effect.

[0083] Table 1. Main aroma components in tobacco paste with boiling points between 100-250℃ (μg / g)

[0084]

[0085] The sensory results of adding bio-based tobacco paste to heated cigarettes are shown in Table 2. The results indicate that the effect of unfermented tobacco paste is generally limited, while tobacco paste fermented with a single strain has a certain effect, improving aroma and aftertaste. However, the bio-based tobacco paste fermented with a combination of strains has a more pronounced aroma, a more harmonious smoke, and the most significant improvement in aftertaste, resulting in the highest overall score.

[0086] Table 2 Sensory evaluation of bio-tobacco paste added to heated cigarettes

[0087]

[0088] The full marks for each indicator are as follows: aroma 32, harmony 6, off-flavors 12, irritation 20, aftertaste 25, and total score 95.

[0089] Although the present invention has been disclosed above with reference to embodiments, it is not intended to limit the present invention. Any person skilled in the art can make various different choices and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention is defined by the claims and their equivalents.

Claims

1. A biological tobacco paste, characterized in that, The bio-toxin paste is made from Klebsiella variegata, with accession number CGMCC No. 29654. Klebsiella variicola Enterobacteriaceae HNYJ-3 and those with accession number CGMCC No. 29655 Enterobacter sp. It is made from HNYJ-4 mixed fermented tobacco; The bio-tobacco paste is prepared by the following method: (1) Preparation of flue-cured tobacco water extract, burley tobacco water extract and aromatic tobacco water extract: The middle tobacco leaves of flue-cured tobacco, burley tobacco and aromatic tobacco are crushed and passed through a 50-mesh sieve to obtain tobacco powder; the tobacco powder is then mixed with pure water to extract the water extracts of flue-cured tobacco, burley tobacco and aromatic tobacco respectively. (2) Prepare seed cultures of Klebsiella variegata HNYJ-3 and Enterobacter HNYJ-4 respectively for later use; (3) Preparation of mixed slurry: The flue-cured tobacco water extract, burley tobacco water extract and spice tobacco water extract from step (1) are mixed in a volume ratio of (5-10):(1-5):(1-5), and the mixture is then mixed with coffee chlorogenic acid in a volume ratio of 1:10-1:100 to obtain mixed slurry; (4) Preparation of fermentation mixed slurry: The Klebsiella variegata HNYJ-3 seed liquid prepared in step (2) is mixed with Enterobacter HNYJ-4 seed liquid in a volume ratio of 1:10-10:1 to obtain a composite seed liquid; (5) Mix the composite seed liquid from step (4) with the mixed slurry from step (2) at a volume ratio of 1:1000-1:100, and ferment at a constant temperature of 25-40℃ for 12-96 h with shaking at a speed of 180 rpm. Centrifuge and collect the supernatant to obtain the fermented mixed slurry. Concentrate the fermented mixed slurry under reduced pressure at 60℃ to 1 / 10 of the original volume to obtain the concentrated slurry. (6) The concentrated slurry is mixed with anhydrous ethanol at a mass ratio of 2:1-1:10, and centrifuged to obtain crude biological tobacco paste. The crude biological tobacco paste is concentrated under reduced pressure to obtain biological tobacco paste.

2. The method for preparing bio-tobacco paste according to claim 1, characterized in that, Includes the following steps: (1) Preparation of flue-cured tobacco water extract, burley tobacco water extract and aromatic tobacco water extract: The middle tobacco leaves of flue-cured tobacco, burley tobacco and aromatic tobacco are crushed and passed through a 50-mesh sieve to obtain tobacco powder; the tobacco powder is then mixed with pure water to extract the water extracts of flue-cured tobacco, burley tobacco and aromatic tobacco respectively. (2) Prepare seed cultures of Klebsiella variegata HNYJ-3 and Enterobacter HNYJ-4 respectively for later use; (3) Preparation of mixed slurry: The flue-cured tobacco water extract, burley tobacco water extract and spice tobacco water extract from step (1) are mixed in a volume ratio of (5-10):(1-5):(1-5), and the mixture is then mixed with coffee chlorogenic acid in a volume ratio of 1:10-1:100 to obtain mixed slurry; (4) Preparation of fermentation mixed slurry: The Klebsiella variegata HNYJ-3 seed liquid prepared in step (2) is mixed with Enterobacter HNYJ-4 seed liquid in a volume ratio of 1:10-10:1 to obtain a composite seed liquid; (5) Mix the composite seed liquid from step (4) with the mixed slurry from step (2) at a volume ratio of 1:1000-1:100, and ferment at a constant temperature of 25-40℃ for 12-96 h with shaking at a speed of 180 rpm. Centrifuge and collect the supernatant to obtain the fermented mixed slurry. Concentrate the fermented mixed slurry under reduced pressure at 60℃ to 1 / 10 of the original volume to obtain the concentrated slurry. (6) The concentrated slurry is mixed with anhydrous ethanol at a mass ratio of 2:1-1:10, and centrifuged to obtain crude biological tobacco paste. The crude biological tobacco paste is concentrated under reduced pressure to obtain biological tobacco paste.

3. The method for preparing bio-tobacco paste according to claim 2, characterized in that, The conditions for preparing the aqueous extract in step (1) are as follows: flue-cured tobacco powder, burley tobacco powder, and aromatic tobacco powder are mixed with pure water at a ratio of 1:20 to 1:5, extracted at a constant temperature of 30-100℃ for 1-10 h, and filtered through 200-mesh gauze to obtain the three aqueous extracts.

4. The method for preparing the bio-tobacco paste according to claim 2, characterized in that, The seed culture preparation in step (2) involves inoculating Klebsiella variegata HNYJ-3 and Enterobacter hNYJ-4 into LB liquid medium and culturing at 30°C for 24 h, respectively, to obtain the OD... 600 A pH of 1.0-3.0 indicates that it is seed liquid.

5. The method for preparing bio-tobacco paste according to claim 2, characterized in that, Step (3) includes: The flue-cured tobacco aqueous extract, burley tobacco aqueous extract and spice tobacco aqueous extract prepared in step (1) are mixed in a volume ratio of 7:2:1 to obtain a mixed tobacco aqueous extract. Tobacco aqueous extract and coffee chlorogenic acid were mixed at a volume-to-mass ratio of 1:50 to obtain a mixed slurry.

6. The method for preparing bio-tobacco paste according to claim 2, characterized in that, Step (4) specifically involves mixing the Klebsiella variegata HNYJ-3 seed solution prepared in step (2) with the Enterobacter HNYJ-4 seed solution at a volume ratio of 2:1 to obtain a composite seed solution.

7. The method for preparing the bio-tobacco paste according to claim 2, characterized in that, In step (5), the composite seed liquid and the mixed slurry are mixed at a volume ratio of 1:200 and fermented at a constant temperature of 30°C for 48 h.

8. The method for preparing the bio-tobacco paste according to claim 2, characterized in that, Step (6) is as follows: the concentrated slurry is mixed with anhydrous ethanol at a mass ratio of 1:2, and then left to stand at -20℃ for 24 h. After centrifugation, crude biological tobacco paste is obtained. The crude biological tobacco paste is concentrated under reduced pressure at 60℃ to 1 / 5 of its original volume to obtain biological tobacco paste.

9. The application of the bio-tobacco paste according to claim 1 or the bio-tobacco paste prepared by the preparation method according to claims 2-8 in heated cigarettes, characterized in that, The bio-tobacco paste is used to enhance the aroma quality and sensory experience of heated cigarettes.

10. A method for improving the sensory quality of heated cigarettes, characterized in that, The method involves adding the bio-tobacco paste as described in claim 1 to heated cigarettes to improve their aroma, balance, reduce off-flavors, decrease irritation, and enhance the aftertaste.

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