TaADA2 protein related to wheat grain storage protein and starch content and application of TaADA2 protein

CN119119221APending Publication Date: 2024-12-13CHINA AGRI UNIV
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Patent Information

Application Number
CN202410852289.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-06-28
Publication Date
2024-12-13

AI Technical Summary

Technical Problem

The prior art is difficult to effectively analyze the gene regulation mechanism of wheat grain starch and storage proteins, affecting wheat yield and quality.

Method used

By cloning the gene TaADA2, which interacts with histone acetylation transferase TaGCN5 in wheat, encoding a transcriptional co-activator, the TaADA2 transgenic material was knocked out using the CRISPR-Cas9 system to analyze its role in the synthesis of wheat grain starch and storage proteins, and KASP molecular markers were developed to identify excellent allelic variants.

Benefits of technology

The important role of TaADA2 in the synthesis of wheat grain starch and storage proteins was clarified, and it was found that excellent allelic variant Hap2 can increase wheat grain weight. The developed KASP marker-assisted selection helps wheat breeding.

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Abstract

The invention relates to wheat grain storage protein and starch content related TaADA2 protein and application thereof, and TaADA2 affects expression of wheat grain starch genes and storage protein genes and is an important gene affecting wheat processing quality. Two haplotypes are found in a coding region of TaADA2-5B, and the two haplotypes are named as TaADA2-5B-Hap1 and TaADA2-5B-Hap2. Compared with the Hap1, the Hap2 has variation in the 532th amino acid sequence and the 538th amino acid sequence of a coding region respectively. A test result shows that the grain length, the grain width and the thousand grain weight of the wheat germplasm resource carrying the Hap1 allelic variation are obviously lower than those of the wheat germplasm resource carrying the Hap2 allelic variation. The result shows that Hap2 may be an excellent allelic variation type for improving the grain weight of wheat.
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Citation Information

Patent Citations

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  • Wheat grain quality character related gene TaPrp-3A molecular marker and application thereof

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