Method for efficiently extracting cordyceps polysaccharide for repairing glomerular injury from cordyceps sobolifera spore powder and application of cordyceps polysaccharide

Abstract

The invention discloses a method for efficiently extracting cordyceps polysaccharide for glomerular injury repair from cordyceps sobolifera spore powder and application. The method comprises the following steps: S1, collecting the cordyceps sobolifera spore powder; s2, the cordyceps sobolifera spore powder is crushed and passes through a 100-200-mesh screen; s3, the smashed cordyceps sobolifera spore powder is added into a solvent with the volume 10 times that of the smashed cordyceps sobolifera spore powder, the solvent is a mixed solvent of distilled water, ethyl alcohol, plant extracts and nano-particles, ultrasonic extraction is conducted for 0.5-1 h at the temperature of 30-40 DEG C, and a magnetic stirrer is used for stirring in the extraction process. According to the invention, the plant extract contains natural surfactants and antioxidants and can assist in extracting and protecting the polysaccharide, the nanoparticles can be used as carriers to help the polysaccharide to be better dissolved and improve the extraction efficiency, and the mixed solvent of distilled water and ethanol is used, so that the solubility of the polysaccharide can be improved, and the extraction efficiency is further improved; the addition of ethanol is beneficial to improving the polarity of the solvent, dissolving some components and improving the extraction efficiency.

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