Construction method and application of genetically engineered bacterium for producing ectoine

CN119120536AActive Publication Date: 2024-12-13ZHEJIANG SEEDLING BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202411616610.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-13
Publication Date
2024-12-13
Estimated Expiration
2044-11-13

AI Technical Summary

Technical Problem

The existing tetrahydropyrimidine fermentation technology relies on expensive inducers and high-cost carbon sources, and it is difficult to effectively utilize cheap agricultural waste as carbon sources, resulting in high production costs and waste of resources.

Method used

By constructing the Halobacillus halophilus DSM 2266 tetrahydropyrimidine synthesis gene cluster and knocking out the lacI gene of E. coli BL21, the phosphate transferase system was modified, and corn core hydrolysate was used as a carbon source to achieve efficient biosynthesis of tetrahydropyrimidine, and tryptophan The acid regulation system regulates product synthesis without adding inducers.

Benefits of technology

High purity (>99%) and high efficiency tetrahydropyrimidine production is achieved, the cost of fermentation materials is reduced, cheap agricultural waste resources are utilized, and the economicality of production and the efficiency of resource recycling is improved.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses a construction method and application of an ectoine-producing genetically engineered bacterium, and belongs to the field of biosynthesis. Escherichia coli BL21 is used as an original strain, lacI genes of the Escherichia coli BL21 are knocked out, an expression system regulated and controlled by tryptophan is constructed, and synthesis of a product ectoine can be regulated and controlled under the condition that an inducer is not added. According to the invention, a phosphotransferase system of Escherichia coli BL21 is modified, and a glucose readifier protein coding gene Zmglf is integrated at a ptsG gene locus, so that the inhibition effect of carbon catabolism is relieved, and the cost of a culture medium is reduced. Through overexpression of a phosphoenolpyruvate carboxylase coding gene ppc and an aspartate semialdehyde dehydrogenase coding gene asd, the accumulation of precursor substances oxaloacetic acid and L-aspartate-beta-semialdehyde in the growth and metabolism process is enhanced, and the synthesis level of the product ectoine is improved.
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Citation Information

Patent Citations

  • Construction method of genetic engineering strain for producing shikimic acid

    CN102304539A

  • Three-gene co-expression vector for synthesizing tetrahydropyrimidine and application of co-expression vector

    CN107475282A

  • Method for accurately and quantitatively regulating and controlling exocytosis level of escherichia coli recombinant protein and application

    CN114686506A

  • Gene recombination strain with high yield of ectoine as well as construction method and application of gene recombination strain

    CN116904379A

  • Genetically modified microorganism for producing ectoine

    WO2024223833A1