HPV vaccine carrying CAR structure, preparation method and application thereof

By designing a HPV vaccine carrying CAR structures of multiple HPV virus subtypes E7 single-chain antibodies and a lipid nanoparticle-encapsulated recombinant plasmids with CD8 antibodies, the problem of poor therapeutic effect of existing vaccines on multiple HPV virus subtypes is solved, and CAR-T cells are directly generated in the body and efficiently resisting infections of multiple HPV virus subtypes.

CN119139462BActive Publication Date: 2025-03-28SHANDONG RES INST OF ADULT CELL IND TECH CO LTD
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Patent Information

Application Number
CN202411657905.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-11-20
Publication Date
2025-03-28
Estimated Expiration
2044-11-20

AI Technical Summary

Technical Problem

The existing HPV vaccine can only prevent HPV infection and has no therapeutic effect on existing infections or lesions. The prepared CAR-T cells target only one HPV virus subtype and fail to target multiple subtypes. There is no report that CAR-T cells in vivo kill HPV viruses.

Method used

Design and construct an HPV vaccine carrying CAR structure. The CAR structure includes E7 single-chain antibodies against multiple HPV virus subtypes. The recombinant plasmid is encapsulated by lipid nanoparticles coupled to CD8 antibodies, so as to effectively capture CD8+ T cells in the body, directly produce CAR-T cells, and resist infection of multiple HPV virus subtypes.

Benefits of technology

The HPV vaccine significantly improved the killing efficiency of Ca Ski and HeLa target cells in vitro. When the target ratio is 20:1, the killing rate reached 93.4% and 91.5%. It also showed significant protective effects in mouse models and could resist infections of various HPV virus subtypes.

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Abstract

The present invention provides an HPV vaccine carrying a CAR structure, a preparation method and an application thereof, which belong to the technical field of medical preparations; the CAR structure includes E7 single-chain antibodies for HPV16, 18, 31, and 33 subtypes and E7 single-chain antibodies for HPV45, 52, 56, 58, and 59 subtypes; the nucleic acid artificial sequence of the E7 single-chain antibody for HPV16, 18, 31, and 33 subtypes is shown in SEQ ID NO.5 in the sequence list; the nucleic acid artificial sequence of the E7 single-chain antibody for HPV45, 52, 56, 58, and 59 subtypes is shown in SEQ ID NO.8 in the sequence list. The HPV vaccine carrying a CAR structure prepared by the present invention effectively captures CD8+T cells in the body to form CAR-T cells, effectively reduces the HPV virus titer in mice, can simultaneously resist the infection of multiple HPV virus subtypes, and has a significant protective effect on mice.
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