A method for improving the genetic transformation efficiency of wheat by using agrobacterium-mediated method

By introducing a recombinant expression vector of the TaHRF2 gene into wheat and using Agrobacterium-mediated transformation to infect wheat embryos, the problem of low genetic transformation efficiency in wheat was solved, achieving efficient gene editing and breeding results.

CN119193687BActive Publication Date: 2026-06-19SHANDONG AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANDONG AGRICULTURAL UNIVERSITY
Filing Date
2024-10-30
Publication Date
2026-06-19

AI Technical Summary

Technical Problem

The wheat genome is large and its chromosomal inheritance is complex. Traditional breeding methods are insufficient to meet the needs of gene function analysis and agronomic trait improvement. Existing transgenic technologies have low genetic transformation efficiency and are limited by genotype, making it difficult to achieve efficient breeding.

Method used

A recombinant expression vector was constructed using the TaHRF2 gene, and wheat embryos were infected using Agrobacterium-mediated transformation to improve the efficiency of nucleic acid integration into the wheat genome and plant regeneration, thereby cultivating transgenic and gene-edited plants.

Benefits of technology

It significantly improved the genetic transformation efficiency of wheat varieties that are difficult to transform, such as 'Beijing No. 8' and 'Lumai No. 1', established an efficient genetic transformation system, and provided technical support for gene function research and breeding.

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Abstract

This invention discloses a method for improving the genetic transformation efficiency of wheat using Agrobacterium-mediated transformation, belonging to the field of plant genetic engineering technology. This invention utilizes the TaHRF2 gene and its encoded protein to promote the introduction of nucleic acid molecules into target plants and improve the genetic transformation efficiency of these molecules. Target plants include, but are not limited to, monocotyledonous plants such as wheat. A TaHRF2 gene high-expression vector is introduced into an Agrobacterium strain, and wheat explants are infected using the Agrobacterium-mediated transformation method. This improves the genetic transformation ability of key parents such as Beijing 8 and Lumai 1, providing important information and foundational technical support for establishing an efficient genetic transformation system for major wheat varieties with regeneration difficulties.
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