PCR (Polymerase Chain Reaction) primer group for simultaneously detecting multiple food pathogenic bacteria, detection system and application
By designing a specific PCR primer set and optimizing the PCR detection system, the problems of low efficiency and high cost of foodborne pathogen detection in the prior art were solved, and the Salmonella, enterohemorrhagic EHEC O157 and Cronobacteria were achieved. Fast and accurate inspection meets the rapid inspection needs of food production and processing.
CN119193873APending Publication Date: 2024-12-27CHINA THREE GORGES UNIV
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Patent Information
- Application Number
- CN202411383464.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-30
- Publication Date
- 2024-12-27
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Figure CN119193873A_ABST
Abstract
The invention relates to the technical field of molecular biological detection, and particularly discloses a PCR (Polymerase Chain Reaction) primer group for simultaneously detecting various food pathogenic bacteria, a detection system and application. Each primer in the primer group is designed and synthesized by taking a z0340 gene of enterohemorrhagic escherichia coli EHEC O157, a ygcB gene of cronobacter and a yhdP gene of salmonella as targets respectively; the z0340 gene of enterohemorrhagic escherichia coli EHEC O157, the ygcB gene of cronobacter and the yhdP gene of salmonella are used as targets; and carrying out PCR amplification on the DNA of the food-borne pathogenic bacteria through the PCR primer group to obtain a detection result. According to the detection system, parallel detection of multiple target genes can be carried out, the detection cost is low, the detection efficiency is greatly improved, and the purpose of simultaneously detecting enterohemorrhagic escherichia coli EHEC O157, cronobacter and salmonella is achieved.
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