inoculator

CN119214038BActive Publication Date: 2026-09-25HEILONGJIANG XINQIAOSHOU TECHNOLOGY DEVELOPMENT CO LTD
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Patent Information

Application Number
CN202411510973.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-10-28
Publication Date
2026-09-25
Estimated Expiration
2044-10-28

AI Technical Summary

Technical Problem

[0004]本发明研发目的是为了解决现有的接菌机无法实现将菌种均匀种入培养基内,接种量会出现不一致,导致培养效果不稳定,同时适用范围窄,自动化程度不高的问题,在下文中给出了关于本发明的简要概述,以便提供关于本发明的某些方面的基本理解

Benefits of technology

[0017]1.本发明可自动完成培养基的接菌操作,无需人工进行接菌操作,自动化程度高,节省劳动力的同时,提升了工作效率;

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Abstract

The utility model belongs to the technical field of agricultural machinery, and relates to a bacteria inoculating machine. The bacteria inoculating machine comprises a frame, a lifting bacteria inoculating mechanism, side clamping and lifting frames, side clamping and lifting cylinders, pneumatic clamping mechanisms and lifting transmission mechanisms. The left and right sides of the lifting bacteria inoculating mechanism are connected to the frame through the lifting transmission mechanisms. The front and rear sides of the frame are arranged with the side clamping and lifting frames. The side clamping and lifting frames are slidingly connected to the frame. The side clamping and lifting frames are provided with the pneumatic clamping mechanisms. The left and right sides of the side clamping and lifting frames are fixedly connected to the side clamping and lifting cylinders. The side clamping and lifting cylinders are fixedly installed on the frame. The bacteria inoculating machine can automatically inoculate bacteria into the culture medium, and the inoculation amount is consistent. The bacteria inoculating machine has a wide application range and high automation degree.
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Description

Technical Field

[0001] This invention relates to a bacterial inoculation machine, belonging to the field of agricultural machinery technology. Background Technology

[0002] Inoculation machines are key pieces of equipment in edible mushroom production, used to inoculate spawn into culture media or bags to ensure rapid mycelial growth and uniform distribution of superior spawn. The design of inoculation machines can improve inoculation efficiency, reduce contamination rates, decrease manual labor, and increase production efficiency. Existing inoculation machines, such as the edible mushroom inoculation machine disclosed in publication number CN103155798A, have a simple structure. They rely solely on the impact force of a cylinder to feed solid spawn into a steel pipe using a mechanical attachment, then the machine moves downwards, punctures the bag, and ejects the spawn into the culture medium. This inoculation method cannot achieve uniform spawn distribution into the culture medium. Under high production volumes, inconsistent inoculation amounts can occur, leading to unstable cultivation results. Furthermore, it has poor adaptability to spawn types, is incompatible with all types of culture media, has a narrow application range, and a low degree of automation.

[0003] Therefore, there is an urgent need to develop a new type of intelligent inoculation machine to solve the above-mentioned technical problems. Summary of the Invention

[0004] The purpose of this invention is to address the problems of existing inoculation machines, such as their inability to evenly inoculate the culture medium, inconsistent inoculation amounts leading to unstable culture results, narrow applicability, and low automation. A brief overview of the invention is provided below to offer a basic understanding of certain aspects of it. It should be understood that this overview is not an exhaustive summary of the invention. It is not intended to identify key or essential parts of the invention, nor is it intended to limit the scope of the invention.

[0005] The technical solution of the present invention:

[0006] The inoculation machine includes a frame, a lifting inoculation mechanism, a side-clamping lifting frame, a side-clamping lifting cylinder, a pneumatic clamping mechanism, and a lifting transmission mechanism. The left and right sides of the lifting inoculation mechanism are connected to the frame through the lifting transmission mechanism. Side-clamping lifting frames are arranged on both the front and rear sides of the frame. The side-clamping lifting frames are slidably connected to the frame. A pneumatic clamping mechanism is installed on the side-clamping lifting frame. The left and right sides of the side-clamping lifting frame are fixedly connected to the side-clamping lifting cylinder, which is fixedly installed on the frame.

[0007] Preferably, the left and right sides of the lifting and inoculating mechanism are slidably connected to the frame through a second auxiliary slide rail group.

[0008] Preferably, the pneumatic clamping mechanism includes a clamping cylinder and a clamping part, the output end of the clamping cylinder is equipped with the clamping part, and the clamping part is machined with an arc-shaped limiting groove.

[0009] Preferably, pneumatic clamping mechanisms are machined on both the left and right sides of the frame.

[0010] Preferably, the lifting transmission mechanism includes a lifting motor, a driving sprocket, a driven sprocket, and a transmission chain. The lifting motor is fixedly mounted on the frame, and a driving sprocket is installed at the output end of the lifting motor. The driven sprocket is rotatably mounted on the frame. The driving sprocket and the driven sprocket are connected through the transmission chain, and the transmission chain is connected to the lifting inoculation mechanism.

[0011] Preferably, a crushing and feeding mechanism is installed on the frame.

[0012] Preferably, the lifting and inoculation mechanism includes a moving frame, a rotary motor, a driving pulley, a driven pulley, a connecting shaft, a rotary feeding part, a transmission belt, and a distributing trough. The rotary motor is fixedly installed on the top of the moving frame, and a driving pulley is installed at the output end of the rotary motor. Multiple driven pulleys are rotatably installed on the moving frame. The driving pulley and the multiple driven pulleys are connected by a transmission belt. A connecting shaft is installed at the bottom of the driven pulleys, and a rotary feeding part is installed at the bottom of the connecting shaft. A distributing trough is installed at the bottom of the moving frame, and the rotary feeding part is located in the distributing trough.

[0013] Preferably, the lifting and inoculation mechanism further includes a movable tray, a screw rod, a screw nut, and a drive motor. Screw rods are rotatably mounted on the four corners of the movable frame via bearings. The top of the screw rod is connected to the output end of the drive motor. A screw nut is fitted on the screw rod. The movable tray is fixedly connected to the screw nut. The dispensing trough is clamped and fixed between the movable tray and the movable frame.

[0014] Preferably, the number of driven pulleys is 16.

[0015] Preferably, the rotary feed unit includes a mounting base, a rotary blade, and a rotary feed spring. The rotary blade and the rotary feed spring are both fixedly mounted on the connecting shaft via the mounting base, and the rotary blade is arranged on one side of the rotary feed spring.

[0016] The present invention has the following beneficial effects:

[0017] 1. This invention can automatically complete the inoculation operation of the culture medium without the need for manual inoculation. It has a high degree of automation, saves labor, and improves work efficiency.

[0018] 2. In this invention, the inoculum bag is clamped and fixed on all four sides during inoculation, which avoids deformation caused by friction during inoculation and improves the production quality of the inoculation process;

[0019] 3. This invention is simple to operate, can be used for batch inoculation production, is adaptable to different production workshops and mushroom bag processing production lines, is highly flexible, and is suitable for widespread application;

[0020] 4. The rotary feeder of the present invention can uniformly inoculate the bacterial strain into the culture medium, and the inoculation amount of a batch of culture medium is uniform and consistent, resulting in stable culture effect. At the same time, it is applicable to a wide range of bacterial strains and has strong practicality. Attached Figure Description

[0021] Figure 1 It is a 3D diagram of the inoculation machine;

[0022] Figure 2 This is the installation diagram for the inoculation machine;

[0023] Figure 3 This is a diagram showing the installation of the lifting transmission mechanism of the present invention;

[0024] Figure 4 This is a schematic diagram of the pneumatic gripping mechanism of the present invention;

[0025] Figure 5 This is a schematic diagram of the pneumatic gripping mechanism of the present invention;

[0026] Figure 6 This is a diagram showing the usage status of the crushing and feeding mechanism and the lifting and inoculation mechanism of the present invention;

[0027] Figure 7 This is a schematic diagram of the lifting and lowering inoculation mechanism of the present invention;

[0028] Figure 8 This is a diagram showing the installation of the driving pulley and the driven pulley of the present invention.

[0029] Figure 9 This is a diagram showing the installation of the lifting inoculation mechanism and the pneumatic clamping mechanism of the present invention.

[0030] Figure 10 This is a diagram showing the assembly of the rotary feeder and the distribution trough of the present invention.

[0031] Figure 11 This is a schematic diagram of the structure of the rotary feeder of the present invention.

[0032] In the diagram: 1-frame, 2-lifting inoculation mechanism, 3-side clamping lifting frame, 4-side clamping lifting cylinder, 5-pneumatic clamping mechanism, 6-lifting transmission mechanism, 7-first auxiliary slide rail group, 8-second auxiliary slide rail group, 9-crushing and feeding mechanism, 10-conveying mechanism;

[0033] 21-Moving frame, 22-Rotary motor, 23-Driving pulley, 24-Driven pulley, 25-Connecting shaft, 26-Rotary feed section, 27-Transmission belt, 28-Distribution trough, 28-Moving disc, 29-Moving support, 210-Lead screw, 211-Lead nut, 212-Drive motor, 261-Mounting base, 262-Rotary blade, 263-Rotary feed spring;

[0034] 51-Clamping cylinder, 52-Clamping part, 53-Arc-shaped limiting groove, 61-Lifting motor, 62-Drive sprocket, 63-Driven sprocket, 64-Transmission chain. Detailed Implementation

[0035] To make the objectives, technical solutions, and advantages of this invention clearer, the invention is described below with reference to specific embodiments shown in the accompanying drawings. However, it should be understood that these descriptions are merely exemplary and not intended to limit the scope of the invention. Furthermore, descriptions of well-known structures and technologies are omitted in the following description to avoid unnecessarily obscuring the concept of the invention.

[0036] The connections mentioned in this invention are divided into fixed connections and detachable connections. Fixed connections, also known as non-detachable connections, include but are not limited to conventional fixed connection methods such as folded connections, riveted connections, adhesive connections, and welded connections. Detachable connections include but are not limited to conventional disassembly methods such as threaded connections, snap-fit ​​connections, pin connections, and hinged connections. When a specific connection method is not explicitly defined, it is assumed that at least one existing connection method can always be found to achieve the function, and those skilled in the art can choose according to their needs. For example, a welded connection can be chosen for fixed connections, and a hinged connection can be chosen for detachable connections.

[0037] Specific implementation method one: Combining Figures 1-11 This embodiment describes an inoculation machine comprising a frame 1, a lifting inoculation mechanism 2, a side-clamping lifting frame 3, a side-clamping lifting cylinder 4, a pneumatic clamping mechanism 5, a lifting transmission mechanism 6, and a first auxiliary slide rail group 7. The left and right sides of the lifting inoculation mechanism 2 are respectively connected to the frame 1 through the lifting transmission mechanism 6. The front and rear sides of the frame 1 are each provided with a side-clamping lifting frame 3. The side-clamping lifting frame 3 is slidably connected to the frame 1 through the first auxiliary slide rail group 7. The pneumatic clamping mechanism 5 is installed on the side-clamping lifting frame 3. The left and right sides of the side-clamping lifting frame 3 are fixedly connected to the side-clamping lifting cylinder 4. The side-clamping lifting cylinder 4 is fixedly installed on the frame 1.

[0038] The left and right sides of the lifting and inoculating mechanism 2 are slidably connected to the frame 2 through the second auxiliary slide rail group 8. Pneumatic clamping mechanisms 5 are processed on both the left and right sides of the frame 2. There are a total of 4 pneumatic clamping mechanisms 5. From a horizontal perspective, the four pneumatic clamping mechanisms 5 are arranged in a rectangular shape.

[0039] The pneumatic clamping mechanism 5 includes a clamping cylinder 51 and a clamping part 52. The output end of the clamping cylinder 51 is equipped with the clamping part 52. The clamping part 52 is machined with multiple arc-shaped limiting grooves 53. The multiple arc-shaped limiting grooves 53 are continuously arranged in a wavy line. The arc-shaped surface of each arc-shaped limiting groove 53 is an arc-shaped contour surface of the outer wall of the solid mushroom bag.

[0040] The lifting transmission mechanism 7 includes a lifting motor 61, a drive sprocket 62, a driven sprocket 63, and a transmission chain 64. The lifting motor 61 is fixedly mounted on the frame 2. The drive sprocket 62 is installed at the output end of the lifting motor 61. The driven sprocket 63 is rotatably mounted on the frame 2. The drive sprocket 62 and the driven sprocket 63 are connected through the transmission chain 64. The movable frame 21 has ears extending from both the left and right ends. The transmission chain 64 is fixedly connected to the ears of the movable frame 21. When the lifting motor 61 is started, the drive sprocket 62 and the driven sprocket 63 drive the transmission chain 64 to move, and the movable frame 21 connected to the transmission chain 64 also moves up and down accordingly.

[0041] The lifting and inoculation mechanism 2 includes a movable frame 21, a rotary motor 22, a driving pulley 23, a driven pulley 24, a connecting shaft 25, a rotary feeding part 26, a transmission belt 27, and a distributing trough 28. The rotary motor 22 is fixedly installed on the top of the movable frame 21. The driving pulley 23 is installed at the output end of the rotary motor 22. Multiple driven pulleys 24 are rotatably installed on the movable frame 21. The driving pulley 23 and the multiple driven pulleys 24 are connected through the transmission belt 27. The connecting shaft 25 is installed at the bottom of the driven pulleys 24. The rotary feeding part 26 is installed at the bottom of the connecting shaft 25. The distributing trough 28 is installed at the bottom of the movable frame 21. The rotary feeding part 26 is disposed in the distributing trough 28. The lifting and inoculation mechanism 2 also includes a movable tray 29, a screw 210, a screw nut 211, and a drive motor 212. The screw 210 is rotatably mounted on the four corners of the movable frame 21 via bearings. The output end of the drive motor 212 is connected to the top of the screw 210. The screw nut 211 is fitted on the screw 210. The movable tray 29 is fixedly connected to the screw nut 211. The material distribution trough 28 is clamped and fixed between the movable tray 29 and the movable frame 21.

[0042] The number of driven pulleys 24 is 16, and one driving pulley 23 passes through... Figure 8 The transmission belt 27, as shown, or other feasible arrangements, drives the 16 driven pulleys 24 to rotate.

[0043] The material distribution tank 28 is divided into upper and lower halves. The lower half is configured as a conical chamber structure with multiple arrays, and the bottom of the conical chamber structure has a microbial outlet.

[0044] The rotating feed unit 26 includes a mounting base 261, a rotating blade 262, and a rotating feed spring 263. The rotating blade 262 and the rotating feed spring 263 are both fixedly mounted on the connecting shaft 25 via the mounting base 261. The rotating blade 262 is arranged on one side of the rotating feed spring 263. The rotating feed unit 26 is rotatably disposed inside the conical chamber structure. At the same time, the bottom of the rotating feed spring 263 passes through the inoculum outlet of the conical chamber structure. When the driven pulley 24 drives the connecting shaft 25 to rotate, the connecting shaft 25 drives the rotating feed unit 26 to rotate synchronously. The rotating blade 262 and the rotating feed spring 263 work together to puncture the culture medium bag, while the rotating feed spring 263 acts as a feeding auger to evenly implant the inoculum into the culture medium bag.

[0045] The frame 1 is equipped with a crushing and feeding mechanism 9, which is a prior art feeding and crushing mechanism. It has a feeding roller inside, feeds from the top, and has a discharge port at the bottom. The discharge port of the crushing and feeding mechanism 9 passes through the movable frame 21 and communicates with the inside of the material distribution trough 28.

[0046] The frame 1 is also equipped with sensors to monitor the material transmission position.

[0047] A conveying mechanism 10 is provided on the frame 1 directly below the lifting and inoculation mechanism 2. The conveying mechanism 10 is a conventional transportation and transmission platform of existing technology, such as a motor-driven roller type or chain type.

[0048] The specific workflow of this embodiment, i.e., a bacterial inoculation method implemented based on this embodiment, is as follows:

[0049] Step 1: Place 16 culture medium bags into a rectangular basket, in the same arrangement as the driven pulley 34.

[0050] Step 2: Start the conveyor mechanism 10. The solid inoculum bag moves in the rectangular basket with the conveyor mechanism 10 to directly below the lifting inoculum collection mechanism 2.

[0051] Step 3: Activate the side clamping lifting cylinder 4 to lower the side clamping lifting frame 4 to the front and rear sides of the solid mushroom bag;

[0052] Step 4: Activate the clamping cylinder 51 of the pneumatic clamping mechanism 5. The clamping cylinder 51 drives the clamping part 52 to clamp the solid mushroom bag from the front, back, left and right sides.

[0053] Step 5: Start the lifting transmission mechanism 6 to drive the lifting inoculation mechanism 2 to rise until the discharge port of the crushing and feeding mechanism 9 is connected to the distribution tank 28. Then start the crushing and feeding mechanism 9 to transport the inoculum from the crushing and feeding mechanism 9 to the distribution tank 28.

[0054] Step 6: Start the lifting motor 61, so that the lifting transmission mechanism 6 drives the lifting inoculation mechanism 2 to descend until the rotating feed part 26 on the connecting shaft 25 pierces and inserts into the culture medium bag. Start the rotating motor 22 to make the rotating feed part 26 rotate, so that the inoculum in the dispensing trough 28 is evenly implanted into the culture medium bag.

[0055] Step 7: Start the lifting motor 61 to make the lifting transmission mechanism 6 drive the lifting inoculation mechanism 2 to rise until the rotating feeding part 26 is separated from the culture medium bag.

[0056] Step 8: Start the conveying mechanism 10 to move the culture medium bag after inoculation away from the workstation for the next stage of processing.

[0057] It should be noted that in the above embodiments, as long as the technical solutions are not contradictory, they can be permuted and combined. Those skilled in the art can exhaust all possibilities based on the mathematical knowledge of permutation and combination. Therefore, the present invention will not describe the technical solutions after permutation and combination one by one, but it should be understood that the technical solutions after permutation and combination have been disclosed by the present invention.

[0058] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for inoculating bacteria, implemented using an inoculation machine, characterized in that, The inoculation machine includes a frame (1), a lifting inoculation mechanism (2), a side clamping lifting frame (3), a side clamping lifting cylinder (4), a pneumatic clamping mechanism (5), and a lifting transmission mechanism (6). The left and right sides of the lifting inoculation mechanism (2) are respectively connected to the frame (1) through the lifting transmission mechanism (6). The front and rear sides of the frame (1) are provided with side clamping lifting frames (3). The side clamping lifting frames (3) are slidably connected to the frame (1). The pneumatic clamping mechanism (5) is installed on the side clamping lifting frame (3). The left and right sides of the side clamping lifting frame (3) are fixedly connected to the side clamping lifting cylinder (4). The side clamping lifting cylinder (4) is fixedly installed on the frame (1). The frame (1) is equipped with a crushing and feeding mechanism (9); The lifting and inoculation mechanism (2) includes a moving frame (21), a rotary motor (22), an active pulley (23), a driven pulley (24), a connecting shaft (25), a rotary feeding part (26), a transmission belt (27), and a distributing trough (28). The rotary motor (22) is fixedly installed on the top of the moving frame (21). The output end of the rotary motor (22) is equipped with an active pulley (23). Multiple driven pulleys (24) are rotatably installed on the moving frame (21). The active pulley (23) and multiple driven pulleys (24) are connected through the transmission belt (27). A connecting shaft (25) is installed at the bottom of the driven pulleys (24). A rotary feeding part (26) is installed at the bottom of the connecting shaft (25). A distributing trough (28) is installed at the bottom of the moving frame (21). The rotary feeding part (26) is located in the distributing trough (28). The lifting and inoculation mechanism (2) also includes a movable tray (29), a screw (210), a screw nut (211), and a drive motor (212). The four corners of the movable frame (21) are rotatably mounted with screws (210) through bearings. The top of the screw (210) is connected to the output end of the drive motor (212). The screw (210) is fitted with a screw nut (211). The movable tray (29) is fixedly connected to the screw nut (211). The material distribution trough (28) is clamped and fixed between the movable tray (29) and the movable frame (21). The rotary feed unit (26) includes a mounting base (261), a rotary blade (262), and a rotary feed spring (263). The rotary blade (262) and the rotary feed spring (263) are both fixedly mounted on the connecting shaft (25) through the mounting base (261), and the rotary blade (262) is arranged on one side of the rotary feed spring (263). The pneumatic clamping mechanism (5) includes a clamping cylinder (51) and a clamping part (52). The output end of the clamping cylinder (51) is equipped with the clamping part (52), and an arc-shaped limiting groove (53) is machined on the clamping part (52). The lifting transmission mechanism (6) includes a lifting motor (61), a drive sprocket (62), a driven sprocket (63), and a transmission chain (64). The lifting motor (61) is fixedly installed on the frame (1). The output end of the lifting motor (61) is equipped with a drive sprocket (62). The driven sprocket (63) is rotatably installed on the frame (1). The drive sprocket (62) and the driven sprocket (63) are connected through the transmission chain (64). The transmission chain (64) is connected to the lifting inoculation mechanism (2). The number of driven pulleys (24) is 16; The material distribution tank (28) is divided into upper and lower halves. The lower half is set as a conical chamber structure with multiple arrays arranged, and the bottom of the conical chamber structure has a bacterial discharge port. The steps of the inoculation method are as follows: Step 1: Place the culture medium bags into a rectangular basket. There are 16 culture medium bags in the basket. The arrangement is the same as that of the driven pulley (24). Step 2: Start the conveying mechanism (10). The solid inoculum pack moves in the rectangular basket with the conveying mechanism (10) to the direct below the lifting inoculum collection mechanism (2). Step 3: Activate the side clamping lifting cylinder (4) to lower the side clamping lifting frame (3) to the front and rear sides of the solid mushroom bag; Step 4: Start the clamping cylinder (51) of the pneumatic clamping mechanism (5). The clamping cylinder (51) drives the clamping part (52) to clamp the solid mushroom bag from the front, back, left and right sides. Step 5: Start the lifting transmission mechanism (6) to drive the lifting inoculation mechanism (2) to rise until the discharge port of the crushing and feeding mechanism (9) is connected to the distribution trough (28). Then start the crushing and feeding mechanism (9) to transport the inoculum from the crushing and feeding mechanism (9) to the distribution trough (28). Step 6: Start the lifting motor (61) to make the lifting transmission mechanism (6) drive the lifting inoculation mechanism (2) to descend until the rotating feed part (26) on the connecting shaft (25) pierces and inserts into the culture medium bag. Start the rotating motor (22) to make the rotating feed part (26) rotate and evenly implant the bacteria in the dispensing trough (28) into the culture medium bag. Step 7: Start the lifting motor (61) to make the lifting transmission mechanism (6) drive the lifting inoculation mechanism (2) to rise until the rotating feeding part (26) is separated from the culture medium bag; Step 8: Start the conveying mechanism (10) to allow the culture medium bag containing the inoculated bacteria to leave the workstation for the next stage of processing.

2. The inoculation method according to claim 1, characterized in that: The left and right sides of the lifting and inoculating mechanism (2) are connected to the frame (1) by the second auxiliary slide rail group (8).

3. The inoculation method according to claim 2, characterized in that: Pneumatic clamping mechanisms (5) are machined on both the left and right sides of the frame (1).

Citation Information

Patent Citations

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  • Auricularia polytricha inoculation machine

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