Rare earth upconversion red-green-blue fluorescent encoding microspheres and preparation method thereof

By modifying the surface functional groups of olefin-norbornene copolymers and using solution blending, combined with the hydrodynamic approach of the fiber core layer, the problem of poor dispersibility of rare earth upconversion nanocrystals in fluorescently encoded microspheres was solved, achieving efficient preparation of stable multicolor fluorescently encoded microspheres suitable for biological sample detection.

CN119220025BActive Publication Date: 2026-06-16ZHEJIANG UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG UNIV
Filing Date
2024-09-13
Publication Date
2026-06-16

AI Technical Summary

⚠Technical Problem

In existing methods for preparing fluorescently encoded microspheres, rare earth upconversion nanocrystals and olefin-norbornene copolymers are prone to phase separation, leading to nanocrystal agglomeration, which affects the monodispersity and sensitivity of the microspheres, and the preparation process is cumbersome.

⚗Method used

Using a modified olefin-norbornene copolymer as the matrix material, -OH or -NH2 functional groups are attached to its surface through a nucleophilic substitution reaction. By combining solution blending and fiber core hydrodynamics, rare earth upconversion red-green-blue fluorescent encoded microspheres are prepared. The glass transition temperature difference and solvent selective dissolution are used to promote the uniform dispersion of nanocrystals.

🎯Benefits of technology

The monodispersity and particle size uniformity of fluorescently encoded microspheres have been improved, enabling the production of monochromatic and multicolor fluorescently encoded microspheres with ultra-large encoding capacity. These microspheres are suitable for large-scale production, exhibit stable fluorescence performance, and are applicable to biological sample detection.

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Abstract

The application discloses a rare earth up-conversion red-green-blue fluorescent coding microsphere, adopts a modified olefin-norbornene copolymer as a matrix material, and adopts red, blue or green rare earth up-conversion luminescent nanocrystals as a fluorescent coding material. The application further discloses a preparation method of the rare earth up-conversion red-green-blue fluorescent coding microsphere. The modified olefin-norbornene copolymer is blended with the up-conversion luminescent nanocrystals to obtain a uniform blend, and the blend is hot-pressed into a rod-shaped core layer which is inserted into a cladding material to form a preform rod, and then the preform rod is hot-drawn into a fiber, and then heat treatment is carried out to obtain a polymer fluorescent microsphere with a coding function. The method can control the fluorescent type and intensity of the fluorescent coding microsphere and the particle size of the microsphere according to requirements, the preparation process is simple, the method is suitable for large-scale production, the fluorescent performance is stable, and the method can be used for detecting cells, proteins or other molecules in biological samples.
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