An eye care liquid for relieving asthenopia, and a preparation method and application thereof

Abstract

The application relates to the technical field of eye-care liquids, in particular to an eye-care liquid for relieving visual fatigue and a preparation method and application thereof. The eye-care liquid is composed of peptide selenium polysaccharide, hyaluronic acid, vitamin B2, oat extract and water. The effective component of the application is peptide selenium polysaccharide, which has good antioxidant effect, can effectively remove free radicals in the eyes, and protects the cell membrane of the eyes to maintain the normal physiological function of the optic nerve. The peptide selenium polysaccharide can protect the retina and eye tissues from damage by the external environment and blue light, and delay the aging process of the eyes. Meanwhile, the peptide selenium polysaccharide promotes metabolism of the eyes, and is helpful to maintaining the normal function and healthy state of the eyes. The application further adds eye-care components such as hyaluronic acid, vitamin B2 and oat essence, which are helpful to regulating the eye function and relieving visual fatigue. The application can effectively improve visual fatigue, and is suitable for relieving or reducing visual fatigue, eye dryness, pain and other discomforts caused by overuse of eyes due to work, study, humidification, all-night work and the like.

Description

Technical Field

[0001] This invention relates to the field of eye care solution technology, specifically to an eye care solution for relieving eye fatigue, its preparation method, and its application. Background Technology

[0002] With changing modern lifestyles, the widespread use of electronic products has led to a significant increase in screen time, resulting in increasingly serious eye strain problems. Symptoms of eye strain include dry eyes, fatigue, blurred vision, and eye discomfort, which not only affect work efficiency but may also have long-term effects on eye health. While there are many existing eye care products, their effectiveness in relieving eye strain is limited, and some products may pose irritation or safety concerns. Therefore, developing a safe and effective eye care solution to relieve eye strain has become an urgent technical challenge. Summary of the Invention

[0003] The technical problem to be solved by the present invention is to provide an eye care solution that relieves eye fatigue by adding ingredients such as peptide selenium polysaccharide and hyaluronic acid to promote eye nutrition and moisturize the eyes, thereby effectively relieving eye fatigue and preventing dry eyes.

[0004] To achieve the above-mentioned technical objectives, the present invention provides the following technical solution:

[0005] An eye care solution for relieving eye strain, the eye care solution being composed of peptide selenium polysaccharide, hyaluronic acid, vitamin B2, oat extract and water.

[0006] As an improvement, the eye care solution is composed of the following components in parts by weight: 1-10 parts of peptide selenium polysaccharide, 0.01-10 parts of hyaluronic acid, 0.0001-0.01 parts of vitamin B2, 0.1-5 parts of oat extract, and 1000 parts of water.

[0007] As an improvement, the peptide selenium polysaccharide is a complex of bioactive peptides and selenium.

[0008] As an improvement, the molecular weight of the hyaluronic acid is 1200-1500 kDa.

[0009] As an improvement, the oat extract is obtained by a water extraction method.

[0010] As an improvement, the vitamin B2 is pharmaceutical grade.

[0011] The preparation method of the present invention includes the following steps:

[0012] (1) Dissolve hyaluronic acid and peptide selenium polysaccharide in water and stir to obtain solution A;

[0013] (2) Add oat extract and vitamin B2 to solution A in step (1) to obtain solution B;

[0014] (3) Fill solution B under sterile conditions to obtain eye care solution.

[0015] The preparation method of peptide selenium polysaccharide in this invention includes the following steps:

[0016] S1. Selecting and preparing raw materials

[0017] Select a selenium source and a peptide source; the selenium source includes selenium yeast or selenium salt, and the selenium source is pulverized into a fine powder to increase the surface area and improve the extraction efficiency; the peptide source includes whey protein, soy protein or microbial protein;

[0018] S2. Selenium Extraction and Purification

[0019] Selenium was extracted by soaking the selenium source in a dilute acid solution at room temperature. The extract was heated to 40°C and maintained for 1 hour, then cooled to room temperature. The solid residue was separated by centrifugation. Selenium was further purified by ion exchange resin, and the pH was adjusted to 6.0. Selenium was then adsorbed and eluted through a resin column.

[0020] S3. Extraction and purification of peptides

[0021] The peptide source was enzymatically hydrolyzed using protease at pH 8.0 and 37°C for 4-6 hours. The hydrolysate was purified by ultrafiltration, and the permeate rich in bioactive peptides was collected. The bioactive peptides were further purified by reversed-phase chromatography. Mobile phase A was 0.1% trifluoroacetic acid aqueous solution, and mobile phase B was 0.1% trifluoroacetic acid in acetonitrile solution. The gradient elution conditions were 5% B for 0-30 minutes, 30% B for 30-60 minutes, and 50% B for 60-90 minutes, with a flow rate of 1 ml / min.

[0022] S4. The binding of peptides to selenium

[0023] The purified selenium solution was mixed with the bioactive peptide solution at pH 7.0 and 4°C to prevent peptide degradation and selenium reduction. Under stirring, the mixture was gradually heated to room temperature and maintained for 24 hours to promote the binding of selenium and peptide. Reducing agents and chelating agents were added during the reaction to fix the binding of selenium and peptide, forming stable peptide selenium polysaccharides.

[0024] S5: Purification of Peptide Selenium Polysaccharide

[0025] The peptide selenium polysaccharide was further purified using gel permeation chromatography (GPC) to remove unreacted peptides or selenium.

[0026] The advantages of this invention are:

[0027] 1. The active ingredient of this invention is peptide selenium polysaccharide, which has excellent antioxidant effects. It can effectively scavenge free radicals in the eyes, protect the cell membranes of the eyes, and maintain the normal physiological function of the optic nerve. Peptide selenium polysaccharide helps protect the retina and eye tissues from damage caused by the external environment and blue light, delays the aging process of the eyes, maintains good vision, and promotes eye metabolism, thus helping to maintain the normal function and health of the eyes.

[0028] 2. This invention also includes eye-care ingredients such as hyaluronic acid, vitamin B2, and oat extract. Hyaluronic acid has moisturizing and water-locking functions, effectively maintaining the moisture level of the eyeballs, promoting the health of eye tissues, and relieving dryness and discomfort caused by prolonged eye use. Vitamin B2 is an essential coenzyme in cellular energy metabolism, helping to maintain the normal function and energy supply of eye cells, thus supporting normal eye function. Vitamin B2 also helps promote blood circulation in the eyes, improving the oxygen and nutrient supply to eye tissues and relieving eye fatigue caused by prolonged eye use. Oat extract contains anti-inflammatory components, such as oat alkaloids, which can help reduce eye inflammation and redness, relieving discomfort caused by eye fatigue. Oat extract also has skin-soothing properties, reducing eye irritation and itching, providing a comfortable user experience. Detailed Implementation

[0029] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are some embodiments of the present invention, but not all embodiments.

[0030] This invention discloses an eye care solution for relieving eye fatigue, which is composed of peptide selenium polysaccharide, hyaluronic acid, vitamin B2, oat extract and water.

[0031] The eye care solution is composed of the following components in parts by weight: 1-10 parts of peptide selenium polysaccharide, 0.01-10 parts of hyaluronic acid, 0.0001-0.01 parts of vitamin B2, 0.1-5 parts of oat extract, and 1000 parts of water.

[0032] Among them, peptide selenium polysaccharide is a complex of bioactive peptides and selenium, and its preparation method is as follows:

[0033] S1. Selecting and preparing raw materials

[0034] Choose a selenium source and a peptide source; the selenium source includes one of selenium yeast or selenium salt, and the selenium source is pulverized into a fine powder to increase the surface area and improve the extraction efficiency; the peptide source includes whey protein, soy protein or microbial protein.

[0035] S2. Selenium Extraction and Purification

[0036] Selenium was extracted by soaking the selenium source in 0.1M HCl solution at room temperature. The extract was heated to 40°C and maintained for 1 hour, then cooled to room temperature. The solid residue was separated by centrifugation. Selenium was further purified by ion exchange resin. The pH was adjusted to 6.0, and the selenium was adsorbed and eluted through a resin column.

[0037] S3. Extraction and purification of peptides

[0038] The peptide source was enzymatically hydrolyzed using trypsin at pH 8.0 and 37°C for 4-6 hours. The hydrolysate was purified by ultrafiltration, and the permeate rich in bioactive peptides was collected. The bioactive peptides were further purified by reversed-phase chromatography. Mobile phase A was 0.1% trifluoroacetic acid aqueous solution, and mobile phase B was 0.1% trifluoroacetic acid in acetonitrile solution. The gradient elution conditions were 5% B for 0-30 minutes, 30% B for 30-60 minutes, and 50% B for 60-90 minutes, with a flow rate of 1 ml / min.

[0039] S4. The binding of peptides to selenium

[0040] The purified selenium solution was mixed with the bioactive peptide solution at pH 7.0 and 4°C to prevent peptide degradation and selenium reduction. Under stirring, the mixture was gradually heated to room temperature and maintained for 24 hours to promote the binding of selenium and peptide. A reducing agent (ascorbic acid) and a chelating agent (EDTA) were added during the reaction to fix the binding of selenium and peptide, forming a stable peptide-selenopolysaccharide.

[0041] S5: Purification of Peptide Selenium Polysaccharide

[0042] The peptide selenium polysaccharide was further purified using gel permeation chromatography (GPC) to remove unreacted peptides or selenium.

[0043] Example 1

[0044] Weigh 1g of peptide selenium polysaccharide and 1g of hyaluronic acid, and dissolve them in 1000mL of water and stir to obtain solution A; then add 1mg of vitamin B2 and 0.1g of oat extract to solution A to obtain solution B. Fill solution B under aseptic conditions to obtain eye care solution.

[0045] Example 2

[0046] Weigh out 10g of peptide selenium polysaccharide and 10g of hyaluronic acid, and dissolve them in 1000mL of water and stir to obtain solution A; then add 10mg of vitamin B2 and 5g of oat extract to solution A to obtain solution B. Fill solution B under aseptic conditions to obtain eye care solution.

[0047] Example 3

[0048] Weigh out 5g of peptide selenium polysaccharide and 6g of hyaluronic acid, and dissolve them in 1000mL of water and stir to obtain solution A; then add 0.1mg of vitamin B2 and 3g of oat extract to solution A to obtain solution B. Fill solution B under aseptic conditions to obtain eye care solution.

[0049] The eye strain resistance test was conducted using the nursing solution prepared in Example 2.

[0050] Experimental objective:

[0051] To evaluate the effectiveness and comfort of anti-eye fatigue eye care solutions in real-world use, targeting individuals who use electronic devices for extended periods or wear contact lenses.

[0052] Experimental Design:

[0053] Sixty volunteers were selected to participate in the clinical trial. These volunteers included individuals who frequently wore contact lenses or used electronic devices such as computers and mobile phones for extended periods. The volunteers were divided into two groups: an experimental group of 30 participants used the eye drops prepared in Example 2, while a control group of 30 participants used a commercially available lutein eye drops. The testing period was set at 5 days.

[0054] Test method:

[0055] 1) The volunteers first cleaned the container cup of the eye care solution.

[0056] 2) Pour 5-10ml of anti-eye fatigue eye care solution into the cup.

[0057] 3) The volunteer lay face down, placed the container cup tightly around the eyes, and then slowly tilted their head back to allow the contact lens solution to come into contact with their eyes.

[0058] 4) Keep your eyes moist for about 10 seconds, then bend down to remove the container cup and pour out the used contact lens solution.

[0059] 5) Clean the container cup and repeat the above steps for the other eye. After use, wash the container cup and let it air dry.

[0060] Volunteers used eye care solution 1-2 times a day.

[0061] The observation indicators include:

[0062] 1. Improved visual clarity; 2. Changes in eye comfort; 3. Improved eye hydration; 4. Relief of dry eye symptoms; 5. Reduced foreign body sensation in the eyes; 6. Reduction and lessening of redness in the eyes.

[0063] Volunteers gave subjective ratings based on their own physical sensations and symptom improvement, ranging from 1 to 5 points.

[0064] Scoring rules:

[0065] 1 point - No improvement or worsening:

[0066] Volunteers reported no improvement after using the product, or their symptoms worsened. Visual clarity remained unchanged or worsened. Eye comfort and hydration did not improve, or they felt more uncomfortable. Dry eye symptoms did not improve, or they felt drier. Redness in the eyes did not decrease, or it became more noticeable.

[0067] 2 points - Slight improvement:

[0068] Volunteers reported slight improvements after using the product. Visual clarity improved slightly, but the effect was not significant. Eye comfort and hydration improved somewhat, but to a limited extent. Dry eye symptoms were relieved, but the effect was not significant. Redness in the eyes decreased, but the reduction was not substantial.

[0069] 3 points - Moderate improvement:

[0070] Volunteers experienced moderate improvement after using the product. Visual clarity improved significantly, but not optimally. Eye comfort and hydration improved significantly, resulting in satisfactory results. Dry eye symptoms were significantly relieved, but occasional dryness still occurred. Redness in the eyes decreased moderately, showing noticeable improvement.

[0071] 4 points - Significant improvement:

[0072] Volunteers experienced significant improvements after using the product. Visual clarity was significantly improved, approaching or reaching optimal levels. Eye comfort and hydration were excellent, resulting in high levels of satisfaction. Dry eye symptoms were significantly relieved, with almost no dryness remaining. Redness in the eyes was significantly reduced, demonstrating a very noticeable improvement.

[0073] 5 points - Complete improvement:

[0074] The volunteers experienced complete improvement after using the product, with all symptoms resolved. Visual clarity was fully restored, with no discomfort. Eye comfort and hydration were optimal, resulting in extreme satisfaction. Dry eye symptoms were completely relieved, with no dryness remaining. Redness in the eyes completely disappeared, leaving the eyes looking very healthy.

[0075] Test results:

[0076] Improved visual clarity 3.7 3.3 Changes in eye comfort 4.5 3.8 Eye area moisturizing 4.4 3.7 The degree of relief of dry eye symptoms 4.0 3.2 Reduction of foreign body sensation in the eyes 4.2 3.1 Reduction and relief of red blood vessels in the eyes 4.1 3.9 Total Average Rating 4.15 3.5

[0077] The results above show that the user experience of this invention's eye drops was significantly better in the controlled trial than in the control group. Volunteers experienced a significant improvement in visual clarity, eye comfort and hydration, relief of dry eye symptoms, reduction of redness in the eyes, and less of a foreign body sensation. This indicates that the invention can effectively improve eye strain and is suitable for relieving or reducing discomfort such as eye strain, dryness, and pain caused by excessive eye use due to work, study, humidification, or staying up late.

[0078] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. An eye care solution for relieving eye strain, characterized in that, The eye care solution is composed of peptide selenium polysaccharide, hyaluronic acid, vitamin B2, oat extract and water; The eye care solution is composed of the following components in parts by weight: 1-10 parts of peptide selenium polysaccharide, 0.01-10 parts of hyaluronic acid, 0.0001-0.01 parts of vitamin B2, 0.1-5 parts of oat extract, and 1000 parts of water. The peptide selenium polysaccharide is a complex of bioactive peptides and selenium; The oat extract was obtained by water extraction. The preparation method of the peptide selenium polysaccharide includes the following steps: S1. Selecting and preparing raw materials Select selenium source and peptide source; the selenium source includes selenium yeast or selenium salt, and the selenium source is pulverized into fine powder to increase the surface area and improve the extraction efficiency; the peptide source includes whey protein and soy protein; S2. Selenium Extraction and Purification Selenium was extracted by soaking the selenium source in a dilute acid solution at room temperature. The extract was heated to 40°C and maintained for 1 hour, then cooled to room temperature. The solid residue was separated by centrifugation. Selenium was further purified by ion exchange resin, and the pH was adjusted to 6.

0. Selenium was then adsorbed and eluted through a resin column. S3. Extraction and purification of peptides The peptide source was enzymatically hydrolyzed using protease at pH 8.0 and 37°C for 4-6 hours. The hydrolysate was purified by ultrafiltration, and the permeate rich in bioactive peptides was collected. The bioactive peptides were further purified by reversed-phase chromatography. Mobile phase A was 0.1% trifluoroacetic acid aqueous solution, and mobile phase B was 0.1% trifluoroacetic acid in acetonitrile solution. The gradient elution conditions were 5% B for 0-30 minutes, 30% B for 30-60 minutes, and 50% B for 60-90 minutes, with a flow rate of 1 ml / min. S4. The binding of peptides to selenium The purified selenium solution was mixed with the bioactive peptide solution at pH 7.0 and 4°C to prevent peptide degradation and selenium reduction. Under stirring conditions, the mixed solution was gradually heated to room temperature and maintained for 24 hours to promote the binding of selenium and peptides; reducing agents and chelating agents were added during the reaction to fix the binding of selenium and peptides, forming stable peptide selenium polysaccharides. S5. Purification of Peptide Selenium Polysaccharide Gel permeation chromatography was used to further purify the peptide selenium polysaccharide to remove unreacted peptides or selenium.

2. The eye care solution for relieving eye fatigue according to claim 1, characterized in that, The molecular weight of the hyaluronic acid is 1200-1500 kDa.

3. The eye care solution for relieving eye fatigue according to claim 1, characterized in that, The vitamin B2 mentioned is pharmaceutical grade.

4. A method for preparing an eye care solution for relieving eye fatigue as described in claim 1, characterized in that, Includes the following steps: (1) Dissolve hyaluronic acid and peptide selenium polysaccharide in water and stir to obtain solution A; (2) Add oat extract and vitamin B2 to solution A in step (1) to obtain solution B; (3) Fill solution B under sterile conditions to obtain eye care solution.