一种与小麦抗条锈病基因Yr85连锁的KASP分子标记及其应用
By developing a KASP molecular marker closely linked to the wheat stripe rust resistance gene Yr85, and using the XK1B-63 and XK1B-65 primer sets for quantitative real-time PCR amplification, the problem of inaccurate wheat stripe rust gene localization was solved, enabling rapid and convenient identification of disease-resistant plants and improving the breeding process.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- NORTHWEST A & F UNIV
- Filing Date
- 2024-11-13
- Publication Date
- 2026-07-17
AI Technical Summary
Current technologies do not provide precise gene mapping for wheat stripe rust, with most remaining in the initial mapping stage. Furthermore, there is a problem of loss of resistance, which limits the application of resistant varieties and the breeding process.
We developed a KASP molecular marker closely linked to the wheat stripe rust resistance gene Yr85, and used XK1B-63 and XK1B-65 primer sets for quantitative real-time PCR amplification. Combined with KlusterCaller software analysis, we accurately identified plants containing Yr85.
It enables rapid, simple, and accurate detection of wheat plants resistant to stripe rust, promotes the screening of disease-resistant wheat germplasm resources and molecular-assisted breeding, and improves breeding efficiency.
Smart Images
Figure CN119242846B_ABST