Method for obtaining non-embryogenic callus of picea mongolica and genetic transformation of non-embryogenic callus of picea mongolica

CN119256982APending Publication Date: 2025-01-07INNER MONGOLIA AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202411670144.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-21
Publication Date
2025-01-07

AI Technical Summary

Technical Problem

The lack of a stable and efficient genetic transformation system for sandy spruce limits the progress of gene function verification, genetic engineering breeding and gene editing research in this species.

Method used

By optimizing the induction and genetic transformation methods of non-embryo callus in Sandspina, including the use of different types of basic induction medium and hormone concentration ratios, the most suitable induction and proliferation medium is screened to achieve non-embryo healing Efficient acquisition and genetic transformation of wounded tissue.

Benefits of technology

It has realized the rapid and efficient preparation and genetic transformation of non-embryo callus in sandy spruce, providing an efficient, safe and large-scale genetic transformation platform, providing research on the anti-resistance gene function and molecular breeding of sandy spruce. Effective tool.

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Abstract

The invention relates to a method for obtaining a non-embryonic callus of Picea mongolica, which comprises the following steps: A, induction of the non-embryonic callus of Picea mongolica: taking mature zygotic embryos of Picea mongolica as explants, and respectively placing the mature zygotic embryos on different types of basic induction culture mediums for culturing to obtain primary non-embryonic callus; b, optimizing the induction culture medium of the non-embryogenic callus of the picea mongolica: (1) screening out an optimal basic induction culture medium in the basic induction culture mediums; (2) further screening out the optimal induction culture medium with the optimal hormone concentration ratio; c, proliferation of the picea mongolica non-embryogenic callus: placing the primary non-embryogenic callus obtained in the step A in a proliferation culture medium with the same basic culture medium and different hormone ratios for culture and proliferation, screening out the most suitable proliferation culture medium with the optimal hormone concentration ratio, and obtaining the non-embryogenic callus which grows healthily for genetic transformation.
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Citation Information

Patent Citations

  • Somatic embryogenesis method for Picea mongolica

    CN105165618A

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