Shenxiong formula for treating Alzheimer's disease and application thereof

Abstract

The application relates to the technical field of traditional Chinese medicines, and provides a ginseng and rhizoma chuanxiong formula for treating Alzheimer's disease and application, which is prepared from the following traditional Chinese medicines: Renshen 10-12g, Chuanxiong 10-12g, Heshouwu 10-12g, Fuling 10-12g, Danggui 6-8g, Huangqi 6-8g, Beishashen 3-5g, Yuanzhi 3-5g, Baishao 3-5g and Gouqi 3-5g. The combination of the components has the comprehensive effects of tonifying liver and kidney, promoting blood circulation to remove blood stasis, nourishing yin and blood, invigorating the spleen and tonifying qi, etc., aims at the core pathogenesis of liver-kidney yin deficiency type Alzheimer's disease, and achieves the purposes of overall conditioning, stabilizing the disease, slowing down cognition, improving anxiety, and slowing down functional decline.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine preparations, specifically relating to a ginseng and chuanxiong formula for treating Alzheimer's disease and its application. Background Technology

[0002] Alzheimer's disease (AD) is a progressive neurodegenerative disease with a complex pathogenesis involving multiple factors. In Traditional Chinese Medicine (TCM) theory, Alzheimer's disease falls under the categories of "dementia" and "forgetfulness," and is often associated with "liver and kidney yin deficiency." Liver and kidney yin deficiency type Alzheimer's disease is mainly characterized by memory loss, cognitive decline, and emotional instability. TCM emphasizes treating the condition through syndrome differentiation and treatment, harmonizing yin and yang, and nourishing the liver and kidneys to improve the patient's symptoms.

[0003] In Traditional Chinese Medicine (TCM), Alzheimer's disease (AD) is typically categorized into the following types based on its clinical manifestations and etiology: **Liver and Kidney Yin Deficiency Type:** Main symptoms include memory loss, irritability, insomnia, and vivid dreams, often accompanied by hot flashes and night sweats. Treatment focuses on nourishing Yin and tonifying the kidneys. **Spleen and Stomach Deficiency Type:** Symptoms include loss of appetite, indigestion, fatigue, and memory loss. These patients usually require strengthening the spleen and stomach. **Phlegm-Dampness Obstruction Type:** Symptoms include mental confusion, slow thinking, slurred speech, accompanied by excessive phlegm and chest tightness. Treatment focuses on resolving phlegm and unblocking the meridians. **Blood Stasis Type:** Symptoms include memory loss, headache, dizziness, and low mood, possibly with significant mood swings. Treatment focuses on promoting blood circulation and removing blood stasis. **Heart and Spleen Deficiency Type:** Symptoms include palpitations, insomnia, memory loss, and loss of appetite, often accompanied by pale complexion. Treatment emphasizes strengthening the spleen and nourishing the heart.

[0004] Currently, Shenqiong Formula, as one of the classic formulas for treating Alzheimer's disease, is mainly used to improve memory and cognitive function. The ginseng and wolfberry in its formula have tonifying effects and can enhance organ function. However, existing research mainly focuses on the application of its basic formula, lacking specific adjustments and in-depth exploration for Alzheimer's disease caused by liver and kidney yin deficiency. This may limit the effectiveness of Shenqiong Formula when targeting specific symptoms and pathological mechanisms.

[0005] To improve the efficacy of the Shenqiong formula in treating Alzheimer's disease of the liver and kidney yin deficiency type, modifications to its composition could be considered. Introducing other traditional Chinese medicinal herbs with significant yin-nourishing and kidney-tonifying effects could not only enhance the overall efficacy of the formula but also potentially produce more significant effects in improving cognitive behavior and anxiety-like behaviors. Such modifications offer new treatment options for patients with Alzheimer's disease of the liver and kidney yin deficiency type, contributing to improved quality of life and treatment satisfaction. Summary of the Invention

[0006] To address the aforementioned problems, this invention first provides a formula containing Ginseng and Ligusticum striatum for treating Alzheimer's disease and its application.

[0007] This invention provides a ginseng and ligusticum formula for treating Alzheimer's disease, prepared from the following raw materials by weight: ginseng 10-12g, ligusticum chuanxiong 10-12g, he shou wu 10-12g, poria cocos 10-12g, angelica sinensis 6-8g, astragalus membranaceus 6-8g, glehnia littoralis 3-5g, polygala tenuifolia 3-5g, peony root 3-5g, and wolfberry 3-5g.

[0008] In some embodiments, the Shenqiong formula is prepared from the following raw materials by weight: 12g ginseng, 10g Ligusticum chuanxiong, 12g Polygonum multiflorum, 10g Poria cocos, 6g Angelica sinensis, 8g Astragalus membranaceus, 5g Adenophora stricta, 5g Polygala tenuifolia, 5g Paeonia lactiflora, and 5g Lycium barbarum.

[0009] In some embodiments, the Shenqiong formula is prepared from the following raw materials by weight: 12g ginseng, 12g Ligusticum chuanxiong, 10g Polygonum multiflorum, 10g Poria cocos, 6g Angelica sinensis, 8g Astragalus membranaceus, 5g Adenophora stricta, 3g Polygala tenuifolia, 3g Paeonia lactiflora, and 5g Lycium barbarum.

[0010] In some embodiments, the Shenqiong formula is prepared from the following raw materials by weight: 12g ginseng, 10g Ligusticum chuanxiong, 12g Polygonum multiflorum, 10g Poria cocos, 8g Angelica sinensis, 6g Astragalus membranaceus, 5g Adenophora stricta, 5g Polygala tenuifolia, 3g Paeonia lactiflora, and 3g Lycium barbarum.

[0011] In some embodiments, the Shenqiong formula is prepared from the following raw materials by weight: 10g ginseng, 12g Ligusticum chuanxiong, 10g Polygonum multiflorum, 12g Poria cocos, 6g Angelica sinensis, 6g Astragalus membranaceus, 3g Adenophora stricta, 5g Polygala tenuifolia, 3g Paeonia lactiflora, and 3g Lycium barbarum.

[0012] In addition, the present invention also provides the application of Shenqiong formula in the preparation of a drug for treating Alzheimer's disease of liver and kidney yin deficiency type.

[0013] The Shenqiong formula of this invention is used for Alzheimer's disease of the liver and kidney yin deficiency type. Its formula includes ginseng, chuanxiong, he shou wu, poria, angelica, astragalus, glehnia littoralis, polygala tenuifolia, white peony root, and wolfberry. The combination of these ingredients works synergistically to tonify the liver and kidneys, promote blood circulation and remove blood stasis, nourish yin and blood, and strengthen the spleen and replenish qi. Targeting the core pathogenesis of Alzheimer's disease of the liver and kidney yin deficiency type, it aims to achieve overall conditioning, stabilize the condition, alleviate cognitive decline, improve anxiety, and slow functional decline.

[0014] Ginseng: Ginseng has the effect of replenishing qi and nourishing yin, which can invigorate qi and strengthen the spleen, while also nourishing the heart and blood. For Alzheimer's disease of the liver and kidney yin deficiency type, ginseng can enhance the overall constitution, improve blood supply to the brain, and improve cognitive and memory functions. It can enhance the patient's energy and disease resistance, help restore normal brain function, replenish qi and blood, improve blood and oxygen supply to the brain, and alleviate fatigue and memory decline caused by qi deficiency.

[0015] Chuanxiong (Ligusticum striatum): Chuanxiong is an important medicinal herb for promoting blood circulation and regulating qi. It can promote blood circulation and improve cerebral microcirculation, and has a good conditioning effect on symptoms of blood stasis caused by liver and kidney yin deficiency. By promoting qi and blood circulation and improving insufficient blood supply to the brain, it helps to alleviate symptoms of cognitive impairment and slow thinking, and relieves the problem of poor blood flow to the brain caused by liver and kidney yin deficiency.

[0016] Polygonum multiflorum (He Shou Wu): Polygonum multiflorum has the effects of tonifying the liver and kidneys, nourishing blood and yin, enhancing kidney essence, and delaying aging. It has a direct conditioning effect on memory decline and slow thinking caused by liver and kidney yin deficiency. It tonifies kidney essence, nourishes liver yin, enhances the supply of brain marrow, alleviates the degeneration of the nervous system, and improves the cognitive function and memory of Alzheimer's patients.

[0017] Poria cocos: Poria cocos has the effects of strengthening the spleen and removing dampness, calming the mind and soothing the nerves. It helps improve insufficient qi and blood production caused by spleen deficiency, thereby indirectly nourishing the brain. At the same time, it can calm the mind and soothe the nerves, helping to alleviate anxiety and mood instability in Alzheimer's patients. It improves spleen deficiency, promotes qi and blood production, and has sedative and calming effects, reducing patients' anxiety, improving sleep quality, and helping to restore cognitive function.

[0018] Angelica sinensis: Angelica sinensis has the effect of nourishing and invigorating blood, regulating qi and blood, and nourishing the brain. For Alzheimer's patients with liver and kidney yin deficiency, Angelica sinensis can replenish heart blood, improve cerebral blood circulation, and alleviate symptoms of insufficient blood supply to the brain. It nourishes and invigorates blood, nourishes brain marrow, improves cognitive function, and alleviates memory loss and poor concentration caused by blood deficiency.

[0019] Astragalus: Astragalus has the effects of tonifying qi and raising yang, strengthening the body's defenses and consolidating the exterior. It can enhance the body's immune function and improve overall blood circulation. For Alzheimer's patients, astragalus can improve blood and oxygen supply to the brain. Strengthening the body, improving immunity, and simultaneously tonifying qi and blood, improving cerebral blood supply, helps to slow down the cognitive decline symptoms of Alzheimer's disease.

[0020] North American ginseng: North American ginseng has the effect of nourishing yin and moistening the lungs, replenishing yin fluids and improving symptoms of yin deficiency. For Alzheimer's disease of the liver and kidney yin deficiency type, North American ginseng helps restore the balance of yin fluids and alleviate the decline in brain function caused by yin deficiency. It nourishes the yin fluids of the liver and kidneys, improves dizziness, tinnitus, insomnia, and cognitive decline caused by yin deficiency, and helps improve the overall health of patients.

[0021] Polygala tenuifolia: Polygala tenuifolia is a well-known sedative and cognitive-enhancing herb. It can calm the mind, soothe the nerves, and improve cognitive function. It has a direct effect on improving cognitive impairment, anxiety, and memory loss in Alzheimer's disease patients with liver and kidney yin deficiency. It calms the mind, enhances cognitive function, and improves mood fluctuations in Alzheimer's patients, while alleviating insomnia and anxiety symptoms caused by liver and kidney yin deficiency.

[0022] White peony root: White peony root has the effects of nourishing blood and astringing yin, calming the liver and relieving pain. It can alleviate symptoms of liver yang hyperactivity caused by liver and kidney yin deficiency and help regulate the liver's function of dispersing and draining. For patients with liver and kidney yin deficiency, white peony root can nourish blood and yin, and balance liver function. It nourishes blood and astringes yin, harmonizes liver function, relieves dizziness, insomnia and emotional instability caused by yin deficiency, and helps stabilize the patient's mental state.

[0023] Goji berries: Goji berries nourish the liver and kidneys, improve eyesight, and calm the mind. Rich in various vitamins and antioxidants, they can enhance liver and kidney function and improve yin deficiency constitution. Simultaneously, they can protect nerve cells and alleviate cognitive decline. They nourish the liver and kidneys, enhance the stability of the nervous system, protect brain cells, slow the progression of Alzheimer's disease, and improve patients' memory and learning abilities.

[0024] Compared with existing Chinese medicine formulas, the combination of various medicinal materials in the prescription of this invention realizes the treatment principle of "holistic conditioning" in traditional Chinese medicine:

[0025] Nourishing the liver and kidneys, replenishing essence and marrow: Through the nourishing effects of Polygonum multiflorum and ginseng in the principal herbs, and Adenophora stricta and Lycium barbarum in the adjuvant herbs, this formula nourishes the liver and kidneys and replenishes kidney essence. The kidneys govern bones and produce marrow, and the brain is the sea of ​​marrow. By replenishing kidney essence, the brain marrow can be strengthened, and cognitive and memory functions can be improved.

[0026] Promoting blood circulation and removing blood stasis: The principal herb, Ligusticum chuanxiong, and the assistant herb, Angelica sinensis, promote blood circulation and remove blood stasis, thereby improving cerebral microcirculation, increasing blood and oxygen supply to the brain, and alleviating the problem of insufficient blood supply to the brain. Nourishing yin and blood, balancing yin and yang: The adjuvant herb, Adenophora stricta, and the guiding herb, Paeonia lactiflora, have the effect of nourishing yin and blood, helping to restore the balance of yin fluid in the body, alleviating the yin-yang imbalance caused by liver and kidney yin deficiency, stabilizing emotions, and improving insomnia, anxiety, and mood swings.

[0027] Strengthening the spleen and replenishing qi, calming the mind and stabilizing the will: Ginseng and Poria have the effect of replenishing qi and strengthening the spleen, improving the insufficient production of qi and blood caused by qi deficiency, helping to nourish blood and calm the mind, and assisting in improving cognitive decline and anxiety caused by liver and kidney yin deficiency.

[0028] Comprehensive regulation and protection of neurons: The polygala and wolfberry in the formula can calm the mind and improve intelligence, nourish the liver and kidneys, and protect nerve cells. By regulating the nervous and immune systems, it can slow down the neurodegenerative process of Alzheimer's disease. Detailed Implementation

[0029] To make the technical problems, technical solutions and advantages of the present invention clearer, a detailed description will be given below in conjunction with specific embodiments.

[0030] Example 1

[0031] A ginseng and ligusticum formula for treating Alzheimer's disease is prepared from the following raw materials by weight:

[0032] Chief herbs: Ginseng 12g, Ligusticum chuanxiong 10g, Polygonum multiflorum 12g, Poria cocos 10g;

[0033] Assistant herbs: Angelica sinensis 6g, Astragalus membranaceus 8g;

[0034] Adjuvant herbs: 5g of North American ginseng and 5g of Polygala tenuifolia;

[0035] Used herbs: 5g of white peony root and 5g of wolfberry.

[0036] Example 2

[0037] A ginseng and ligusticum formula 2 for treating Alzheimer's disease is prepared from the following raw materials by weight:

[0038] Chief herbs: Ginseng 12g, Ligusticum chuanxiong 12g, Polygonum multiflorum 10g, Poria cocos 10g;

[0039] Assistant herbs: Angelica sinensis 6g, Astragalus membranaceus 8g;

[0040] Adjuvant herbs: 5g of North American ginseng and 3g of Polygala tenuifolia;

[0041] Used herbs: 3g of white peony root and 5g of wolfberry.

[0042] Example 3

[0043] A formula for treating Alzheimer's disease, namely, Ginseng and Ligusticum striatum, is prepared from the following raw materials by weight:

[0044] Chief herbs: Ginseng 12g, Ligusticum chuanxiong 10g, Polygonum multiflorum 12g, Poria cocos 10g;

[0045] Assistant herbs: Angelica sinensis 8g, Astragalus membranaceus 6g;

[0046] Adjuvant herbs: 5g of North American ginseng and 5g of Polygala tenuifolia;

[0047] Used herbs: 3g of white peony root and 3g of wolfberry.

[0048] Example 4

[0049] A formula for treating Alzheimer's disease, namely, Ginseng and Ligusticum striatum, is prepared from the following raw materials by weight:

[0050] Chief herbs: Ginseng 10g, Ligusticum chuanxiong 12g, Polygonum multiflorum 10g, Poria cocos 12g;

[0051] Assistant herbs: Angelica sinensis 6g, Astragalus membranaceus 6g;

[0052] Adjuvant herbs: 3g of North American ginseng and 5g of Polygala tenuifolia;

[0053] Used herbs: 3g of white peony root and 3g of wolfberry.

[0054] Example 5

[0055] The preparation method of the Ginseng and Ligusticum formula 1-4 for treating Alzheimer's disease is as follows:

[0056] Step 1: Pre-treatment of medicinal materials

[0057] Raw material preparation: Weigh the raw materials recorded in Examples 1-4, confirm that the quality meets the standards, and remove impurities such as dust and sand.

[0058] Cleaning: Quickly rinse the medicinal materials involved in Examples 1-4 with clean water to remove surface adhering substances, and drain the water as much as possible after cleaning.

[0059] Drying process: Place the cleaned medicinal materials in a drying oven at about 60℃ to dry them, ensuring that the moisture content is below 10% to facilitate subsequent pulverization and extraction.

[0060] Step 2: Crushing

[0061] Coarse powder pulverization: The dried medicinal materials are coarsely pulverized to obtain coarse granular medicinal powder.

[0062] Fine powder pulverization: The coarse powdered medicinal materials are further processed using an ultra-micro pulverizer to pulverize them into a fine powder state, so that the powder particle size is between 80-100 mesh, in order to increase the surface area of ​​the medicinal materials and facilitate the subsequent extraction of effective components.

[0063] Step 3: Extract the active ingredients

[0064] Extraction solvent selection: Based on the medicinal properties of the medicinal materials, 70% ethanol was selected as the extraction solvent.

[0065] Extraction method: Place the pulverized medicinal materials in an extraction tank, add 70% ethanol, and control the liquid-to-material ratio at 10:1 (10 times the volume of ethanol).

[0066] Heat to 70°C and maintain extraction for 1.5 hours for the first extraction.

[0067] The first extract was obtained by filtration. The residue was added to 70% ethanol and heated to 70°C again for 1 hour for a second extraction.

[0068] The two extracts were combined, allowed to stand for 12 hours to settle, and then further filtered to remove impurities, resulting in a clear extract.

[0069] Step 4: Concentration and Drying

[0070] Concentration: Place the extract in a rotary evaporator and concentrate it under reduced pressure (50-60℃) until it becomes a thick paste, with the solvent residue controlled below 5%.

[0071] Spray drying: The concentrated paste is dried using a spray dryer at a temperature controlled at 60-80℃ to obtain dried fine powder extracts of the Chinese herbs 1-4 from the Ginseng and Ligusticum formula.

[0072] Example 6: Experiment on the improvement of cognitive behavior and anxiety-like behavior in Alzheimer's mice with liver and kidney yin deficiency syndrome using Shenqiong formula 1-4.

[0073] Healthy C57BL / 6 mice, 6-8 weeks old, of any sex and weighing 18-22g, were induced to develop a liver and kidney yin deficiency type Alzheimer's disease model by injection of aluminum chloride and D-galactose combined with a high-fat diet, and the modeling was maintained for 8 weeks.

[0074] Alzheimer's disease induction: D-galactose (120 mg / kg) was administered intraperitoneally once daily for 8 weeks to mimic the pathological process of Alzheimer's disease. D-galactose induced oxidative stress and neurotoxicity, resulting in memory and cognitive impairment in mice.

[0075] The kidney-yang deficiency model was established based on D-galactose injection. Mice were given the following interventions to induce kidney-yang deficiency:

[0076] Subcutaneous injection of hydrocortisone (10 mg / kg) once daily for 4 weeks. Hydrocortisone inhibits adrenal cortex function, leading to symptoms related to kidney yang deficiency. Furthermore, switching the model mice to a high-fat diet exacerbated these symptoms.

[0077] The mouse model of Alzheimer's disease with liver and kidney yin deficiency syndrome exhibited Alzheimer's disease-like pathological features and cognitive impairment, accompanied by symptoms of liver and kidney yin deficiency under the diagnosis of traditional Chinese medicine.

[0078] Mice were randomly divided into the following 5 groups, with 12 mice in each group:

[0079] Normal control group: Normal mice were given saline solution without any treatment;

[0080] Model group: Alzheimer's disease model mice with liver and kidney yin deficiency syndrome were treated with physiological saline without drug intervention;

[0081] Group 1 of Ginseng and Ligusticum Formula: Alzheimer's disease model mice with liver and kidney yin deficiency were given Formula 1 once a day at a dose of 1000 mg / kg for 8 consecutive weeks (once a day) by gavage.

[0082] Group 2 of Ginseng and Ligusticum Formula: Alzheimer's disease model mice with liver and kidney yin deficiency were given Formula 2 once a day at a dose of 1000 mg / kg for 8 consecutive weeks (once a day) by gavage.

[0083] Group 3 of the Ginseng and Ligusticum Formula: Alzheimer's disease model mice with liver and kidney yin deficiency were given Formula No. 3 once a day at a dose of 1000 mg / kg for 8 consecutive weeks (once a day) by gavage.

[0084] Group 4 of the Ginseng and Ligusticum Formula: Alzheimer's disease model mice with liver and kidney yin deficiency were given Formula No. 4 once a day at a dose of 1000 mg / kg for 8 consecutive weeks (once a day) by gavage.

[0085] Positive control group: Alzheimer's disease model mice with liver and kidney yin deficiency were given donepezil at a dose of 2 mg / kg once a day for 8 weeks (once a day) by gavage.

[0086] Morris water maze test evaluates the spatial learning and memory abilities of mice:

[0087] Training phase: Water maze training was conducted for 4 consecutive days, and the latency period for the mice to find the platform was recorded each day.

[0088] Testing phase: On day 5, the platform was removed, and the time the mice spent in the quadrant where the original platform was located and the number of times they crossed it were recorded.

[0089] Observation indicators: escape latency, time spent in the target quadrant, number of times the original platform area is crossed, and cognitive index.

[0090] Table 1. Changes in escape latency in a mouse model of Alzheimer's disease with liver and kidney yin deficiency syndrome as determined by traditional Chinese medicine (x±s, n=12)

[0091]

[0092]

[0093] Note: Compared with the normal control group, a P < 0.05 b P < 0.01; compared with the model group, c P < 0.05 d P < 0.01.

[0094] Table 1 shows that after day 5, compared with the normal control group, the escape latency of mice in the model group was significantly prolonged (P<0.05); compared with the model group, the escape latency of mice in the Shenqiong formula groups 1-2 was significantly shortened (P<0.05); and the escape latency of mice in the Shenqiong formula groups 3-4 was significantly shortened (P<0.01).

[0095] Table 2. Effects of formula-based syndrome differentiation on the percentage of runs in the target quadrant and the number of times the model mice with liver and kidney yin deficiency syndrome (x±s, n=12)

[0096]

[0097] Note: Compared with the normal control group, a P < 0.05 b P < 0.01; compared with the model group,c P < 0.05 d P < 0.01.

[0098] Table 1 shows that, in the space exploration experiment, compared with the normal control group, the number of times mice crossed the platform (P<0.01) and the percentage of the target quadrant run were significantly reduced in the model group (P<0.05); compared with the model group, the number of times mice crossed the platform and the percentage of the target quadrant run were significantly increased in the Ginseng and Ligusticum Formula 1-4 groups and the positive control group (P<0.05, 0.01).

[0099] Table 3. Effects of prescription-based diagnosis on cognitive index in Alzheimer's disease model mice with liver and kidney yin deficiency syndrome (x±s, n=12)

[0100] normal control group 72±12 Model group <![CDATA[32±14 b ]]> Ginseng and Ligusticum Formula 1 <![CDATA[56±10 c ]]> Two sets of Ginseng and Ligusticum formula <![CDATA[63±15 c ]]> Three groups of Ginseng and Ligusticum formula <![CDATA[57±13 d ]]> Four groups of Ginseng and Ligusticum formula <![CDATA[67±14 d ]]> Positive control group <![CDATA[53±11 c ]]>

[0101] Note: Compared with the normal control group, a P < 0.05 b P < 0.01; compared with the model group, c P < 0.05 d P < 0.01.

[0102] Table 3 shows that, in the new object recognition experiment, compared with the normal control group, the cognitive index of mice in the model group was significantly reduced (P<0.01); compared with the model group, the cognitive index of mice in the Shenqiong formula groups 1-4 and the positive control group was significantly increased (P<0.05, 0.01).

[0103] Open field experiments were used to test the mice's spontaneous activity and anxiety levels.

[0104] Mice were placed in the center of an open field experimental setup, and their movement trajectory, total movement distance, number of times they entered the central area, and dwell time were observed over a 10-minute period.

[0105] Observation indicators: distance traveled in the central area, percentage of time spent in the central area, and number of times the central area was entered.

[0106] Table 4. Changes in central region movement distance, percentage of time spent, and number of times entering the central region in Alzheimer's disease model mice with liver and kidney yin deficiency syndrome, based on the formula's diagnostic method (x±s, n=12).

[0107]

[0108]

[0109] Note: Compared with the normal control group, a P < 0.05 b P < 0.01; compared with the model group, c P < 0.05 dP < 0.01.

[0110] Table 4 shows that, in the open field experiment, compared with the normal control group, the percentage of central region movement distance, the percentage of central region movement time, and the number of times entering the central region were significantly reduced in the model group mice (P < 0.05, 0.01); compared with the model group, the percentage of central region movement distance, the percentage of central region movement time, and the number of times entering the central region were significantly increased in the ginseng and ligusticum formula groups 1-4 and the positive control group mice (P < 0.05, 0.01).

[0111] The above description represents the preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A traditional Chinese medicine composition for treating Alzheimer's disease of the liver and kidney yin deficiency type, characterized in that, The traditional Chinese medicine composition is prepared from the following raw materials by weight: ginseng 12g, chuanxiong 10g, he shou wu 12g, poria cocos 10g, angelica sinensis 8g, astragalus membranaceus 6g, glehnia littoralis 5g, polygala tenuifolia 5g, white peony root 3g, and wolfberry 3g.

2. A traditional Chinese medicine composition for treating Alzheimer's disease of the liver and kidney yin deficiency type, characterized in that, The traditional Chinese medicine composition is prepared from the following raw materials by weight: ginseng 10g, chuanxiong 12g, he shou wu 10g, poria cocos 12g, angelica sinensis 6g, astragalus membranaceus 6g, glehnia littoralis 3g, polygala tenuifolia 5g, white peony root 3g, and wolfberry 3g.

3. The use of the traditional Chinese medicine composition according to claim 1 or 2 in the preparation of a medicament for treating Alzheimer's disease of the liver and kidney yin deficiency type.

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