A cerium-based nanozyme and its preparation method and application in organophosphorus pesticide detection

Abstract

The present invention discloses a cerium-based nanozyme and its preparation method and application in the detection of organophosphorus pesticides, belonging to the technical field of pesticide residue detection. The cerium-based nanozyme has a class of organophosphorus hydrolase activity, which includes: a nitrogen-doped carbon nanosheet substrate, and Ce2O2CN2 nanoparticles loaded on the surface of the nitrogen-doped carbon nanosheet substrate. The nanozyme provided by the present invention avoids the use of expensive, difficult to preserve, and harsh reaction conditions of natural enzymes (such as: organophosphorus hydrolase, acetylcholinesterase, alkaline phosphatase, etc.), and realizes low-cost rapid detection of paraoxon. Compared with the reported Ce-based nanozyme, the nanozyme prepared by the present invention has simple preparation method, good stability, small usage, mild reaction conditions (37°C), fast detection speed, wide measurement range, and the test results can be directly displayed by a UV-visible spectrophotometer, which can realize on-site real-time detection, which is conducive to rapid and accurate detection of the pesticide paraoxon.

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