Mstnr and its coding gene and application
By providing the nitroreductase Mstnr and its encoding gene mstnr, the biodegradation of nicosulfuron into inactive products was achieved, solving the problem of weed resistance and demonstrating its value in biodegradation, detoxification, and remediation applications.
CN119286811BActive Publication Date: 2025-10-24NANJING AGRICULTURAL UNIVERSITY
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Patent Information
- Application Number
- CN202411531957.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-30
- Publication Date
- 2025-10-24
- Estimated Expiration
- 2044-10-30
AI Technical Summary
Technical Problem
Long-term use of nicosulfuron leads to weed resistance problems, and existing technologies lack effective methods for nicosulfuron degradation and detoxification.
Method used
A nitroreductase Mstnr and its encoding gene mstnr are provided. By constructing a recombinant expression vector and expressing it in Escherichia coli, the biodegradation of nitroreductone into the inactive product 2-amino-4-methanesulfonylbenzoic acid is achieved.
Benefits of technology
It achieves efficient degradation of mesotrione, restores the activity of the herbicide, solves the problem of weed resistance, and has potential value in biodegradation, detoxification, and remediation applications.
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Abstract
The application belongs to the field of agricultural biotechnology and environmental microbial engineering, and discloses a mesotrione nitroreductase Mstnr, a coding gene thereof and application. The nucleotide sequence is SEQ ID NO. 1, the full length is 678 bp, and 225 amino acids are encoded, and the amino acid sequence is SEQ ID NO. 2. The homology of Mstnr with the reported nitroreductase is only 49.2%, and therefore it is a new nitroreductase gene. The nitroreductase Mstnr provided by the application can catalyze the nitroreduction of mesotrione, and convert mesotrione into a product 2-amino-4-methylsulfonylbenzoic acid (AMBA) without herbicidal activity, indicating that the Mstnr gene has great application prospect in mesotrione biodegradation detoxification and remediation.
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