A composition with skin tenderizing and firming anti-wrinkle efficacy and its use

Abstract

The present application relates to a kind of compositions with skin-tenderizing and firming anti-wrinkle efficacy and its application, the composition with skin-tenderizing and firming anti-wrinkle efficacy includes: schizosaccharomyces pombe ferment filtrate, yeast / rice ferment filtrate and rose water.The schizosaccharomyces pombe ferment filtrate, yeast / rice ferment filtrate and rose water in the composition of the present application are reasonably matched, have synergistic effect, can make the composition have skin-tenderizing, anti-wrinkle and firming skin multiple effects.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a composition with skin-rejuvenating, firming, and anti-wrinkle effects and its application. Background Technology

[0002] The skin consists of three layers: the epidermis, dermis, and subcutaneous tissue. The dermis contains abundant collagen and elastin, proteins that keep the skin elastic and firm. As people age or are affected by external environmental factors, the production of these proteins in the skin gradually decreases, leading to a loss of elasticity and the formation of wrinkles. Wrinkles are the most direct manifestation of skin aging. Therefore, to slow down skin aging, more and more manufacturers are developing anti-wrinkle and firming cosmetics to reduce or prevent wrinkles.

[0003] CN118717555A discloses a composition with firming and anti-wrinkle effects, belonging to the field of cosmetic technology. The composition includes hydroxypropyl tetrahydropyranotriol, hydroxypinazone retinate, gold button extract, hydrolyzed hyaluronic acid, niacinamide, glutathione, carnosine, bisabolol, panthenol, bifida ferment filtrate, aloe vera leaf water, and Rosa damascena flower water. This composition rebuilds the dermal structure of the skin from the inside out, from different dimensions and the basal layer, replenishing hyaluronic acid while promoting the synthesis of hyaluronic acid and collagen, thereby achieving firming and anti-wrinkle skincare effects. It is also stable, safe, and effective.

[0004] CN114748374A discloses an anti-wrinkle composition and its preparation method, relating to the field of cosmetic technology. The anti-wrinkle composition, by weight, comprises the following raw materials: 0.01-5 parts silk peptide, 0.001-2 parts pentapeptide-3, 0.01-3 parts palmitoyl pentapeptide-3, 0.001-0.01 parts idebenone, 1-10 parts lycopene, and 10-20 parts sea fennel extract. The preparation method is as follows: idebenone and sea fennel extract are mixed, glycerin is added, the mixture is stirred and heated, cooled, and then lycopene is added and mixed to obtain a premix; silk peptide, pentapeptide-3, and palmitoyl pentapeptide-3 are mixed, and then added to the premix and stirred. This invention's anti-wrinkle composition can improve skin wrinkles, promote fibroblast proliferation and collagen synthesis, inhibit tyrosinase activity and lipid peroxidation, and has significant anti-wrinkle, whitening, and skin-firming effects. Furthermore, its preparation method is simple, quick, and easy to operate.

[0005] Currently, although the aforementioned cosmetics contain a large number of active ingredients, it is difficult to see obvious effects in a short period of time after the combination of these active ingredients. Therefore, it is necessary to develop a skin care product with more obvious and safer effects, including anti-wrinkle, skin rejuvenation, and skin firming. Summary of the Invention

[0006] In view of the shortcomings of the prior art, the purpose of this invention is to provide a composition with skin-rejuvenating, firming and anti-wrinkle effects and its application.

[0007] To achieve this objective, the present invention adopts the following technical solution:

[0008] In a first aspect, the present invention provides a composition having skin-rejuvenating, firming and anti-wrinkle effects, the composition comprising: Bifida ferment filtrate, yeast / rice ferment filtrate and rose water.

[0009] In the composition of this invention with skin-rejuvenating, firming, and anti-wrinkle effects, the bifida ferment filtrate contains small-molecule amino acids, minerals, and B vitamins, which can capture free radicals, enhance the metabolism of the stratum corneum, shrink pores, and make the skin more delicate; the yeast / rice ferment filtrate is rich in amino acids, proteins, polysaccharides, vitamins, and antioxidants, which can increase the skin's moisture content, form a protective film to lock in moisture, keep the skin soft, smooth, and hydrated, and neutralize free radicals, reduce oxidative damage, promote the regeneration of the epidermis, and make the skin smoother and softer; the rose water has moisturizing properties and is suitable for use when the skin is dry, providing a moisturizing effect. It is also rich in antioxidants such as vitamin C, anthocyanins, and polyphenols, which can effectively combat free radicals and thus have excellent anti-wrinkle effects. The three ingredients, when combined, have a synergistic effect, giving the composition multiple effects such as skin rejuvenation, anti-wrinkle, and skin firming.

[0010] Preferably, the mass ratio of the bifida ferment filtrate, the yeast / rice ferment filtrate, and the rose water is (3-5):(1-4):(0.1-10).

[0011] Among them, "3-5" can be 3.1, 3.3, 3.5, 3.7, 3.9, 4, 4.1, 4.3, 4.5, 4.7 or 4.9, etc.;

[0012] "1-4" can be 1.2, 1.5, 1.8, 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5, or 3.8, etc.

[0013] "0.1-10" can be 0.2, 0.4, 0.6, 0.8, 1, 1.52, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9 or 9.5, etc.

[0014] Preferably, the composition having skin-rejuvenating, firming and anti-wrinkle effects further includes any one or a combination of at least two of hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid or Micrococcus ovalis extract.

[0015] Preferably, the composition having skin-rejuvenating, firming, and anti-wrinkle effects further includes hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, and *Microcystis globulus* extract.

[0016] The hydroxypropyl tetrahydropyranotriol in the composition of this invention contains a large number of hydroxyl (-OH) groups. These hydroxyl groups can absorb surrounding moisture and retain it on the skin surface, thereby preventing moisture loss. It also has a strong antioxidant effect. The tetrahydromethylpyrimidine carboxylic acid and the extract of Microcystis globulus have excellent moisturizing effects, can stabilize the skin cell structure, help the skin lock in moisture and keep it hydrated, and can also resist oxidation and improve skin elasticity. The three work synergistically to further improve the skin-rejuvenating, anti-wrinkle and skin-firming effects of the composition.

[0017] Preferably, the composition having skin-rejuvenating, firming, and anti-wrinkle effects comprises, by weight, 15-25 parts of Bifida ferment filtrate, 5-20 parts of yeast / rice ferment filtrate, 5-10 parts of rose water, 1-2 parts of hydroxypropyl tetrahydropyranotriol, 1-5 parts of tetrahydromethylpyrimidine carboxylic acid, and 0.1-2 parts of *Microcystis globulus* extract.

[0018] The amount of Bifida Ferment Lysate filtrate added to the composition of the present invention having skin-rejuvenating, firming and anti-wrinkle effects can be 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, 23 parts or 24 parts, etc.

[0019] The amount of yeast / rice fermentation product filtrate added can be 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18 or 19 parts, etc.

[0020] The amount of rose water added can be 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, or 9.5 parts, etc.;

[0021] The amount of hydroxypropyl tetrahydropyranotriol added can be 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, or 1.9 parts, etc.;

[0022] The amount of tetrahydromethylpyrimidine carboxylic acid added can be 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, 2.2 parts, 2.5 parts, 2.8 parts, 3 parts, 3.2 parts, 3.5 parts, 3.8 parts, 4 parts, 4.2 parts, 4.5 parts, or 4.8 parts, etc.;

[0023] The amount of the ocular vein-like microphobic algae extract can be 0.2 parts, 0.4 parts, 0.6 parts, 0.8 parts, 1 part, 1.2 parts, 1.4 parts, 1.6 parts, or 1.8 parts, etc.

[0024] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0025] Secondly, the present invention provides the application of the composition having skin-rejuvenating, firming and anti-wrinkle effects as described in the first aspect in the preparation of cosmetics.

[0026] Preferably, the cosmetic includes any one of serum, lotion, cream, facial cleanser, or toner.

[0027] Preferably, the composition having skin-rejuvenating, firming, and anti-wrinkle effects is present in the cosmetic at a mass percentage of 1-15%, for example, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, or 14%.

[0028] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0029] Thirdly, the present invention provides a serum, the components of which include the composition having skin-rejuvenating, firming and anti-wrinkle effects as described in the first aspect, a moisturizer, an emulsifier, a thickener and water.

[0030] Preferably, the components of the essence include 1-15 parts of the composition with skin-rejuvenating, firming and anti-wrinkle effects as described in the first aspect, 10-30 parts of moisturizer, 1-10 parts of emulsifier, 0.1-2 parts of thickener and 45-90 parts of water.

[0031] The amount of the composition with skin-rejuvenating, firming and anti-wrinkle effects added to the essence of the present invention can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts or 14 parts, etc.

[0032] The amount of the humectant added can be 11 parts, 13 parts, 15 parts, 17 parts, 19 parts, 20 parts, 23 parts, 25 parts, 27 parts, or 29 parts, etc.

[0033] The amount of emulsifier added can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, or 9 parts, etc.

[0034] The amount of the thickener added can be 0.3 parts, 0.5 parts, 0.7 parts, 0.9 parts, 1 part, 1.2 parts, 1.5 parts, 1.7 parts, or 1.9 parts, etc.;

[0035] The amount of water added can be 50 parts, 55 parts, 60 parts, 65 parts, 70 parts, 75 parts, 80 parts, or 85 parts, etc.

[0036] Preferably, the moisturizer comprises any one or a combination of at least two of mucopolysaccharides, polypeptides, or complex ceramides.

[0037] Preferably, the moisturizer includes mucopolysaccharides, polypeptides, and complex ceramides.

[0038] In this invention, the combination of mucopolysaccharides, polypeptides and complex ceramides can provide more nutrients and energy to skin cells, and deeply moisturize the skin. When added to the serum, it can work synergistically with the composition that has skin-rejuvenating, firming and anti-wrinkle effects to further improve the moisturizing and skin-rejuvenating effect of the serum.

[0039] Preferably, the mass ratio of the mucopolysaccharide, polypeptide, and complex ceramide is (0.5-5):(0.5-10):(1-5).

[0040] The values ​​“0.5-5” can be 0.8, 1, 1.2, 1.5, 1.7, 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5, or 4.8, etc.

[0041] "0.5-10" can be 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9 or 9.5, etc.

[0042] “1-5” can be 1.2, 1.5, 1.7, 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5 or 4.8, etc.

[0043] Preferably, the composite ceramide includes ceramide 2, ceramide 3, ceramide 6 and ceramide 7.

[0044] Preferably, the emulsifier comprises polyglycerol esters and / or PEG / PPG-14 / 7 dimethyl ether.

[0045] Preferably, the emulsifier includes polyglycerol esters and PEG / PPG-14 / 7 dimethyl ether.

[0046] Preferably, the mass ratio of the polyglycerol ester to PEG / PPG-14 / 7 dimethyl ether is (1-2):(0.1-1).

[0047] "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, or 1.9, etc.

[0048] "0.1-1" can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 or 0.9, etc.

[0049] Preferably, the polyglycerol esters include polyglycerol fatty acid esters and / or polyglycerol ricinoleate.

[0050] Preferably, the thickener comprises any one or a combination of at least two of xanthan gum, guar gum, hydroxyethyl cellulose, or sodium acrylate / sodium acryloyl dimethyl taurate copolymer.

[0051] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0052] Fourthly, the present invention provides a method for preparing an essence as described in the third aspect, the method comprising:

[0053] (1) Aqueous liquid is obtained by stirring water and humectant, heating and keeping warm; oil liquid is obtained by stirring emulsifier and thickener, heating and keeping warm.

[0054] (2) After the heat preservation is completed, the aqueous phase liquid and the oil phase liquid are mixed and homogenized, cooled down, and the composition with skin-rejuvenating, firming and anti-wrinkle effects is added. After homogenization, the essence is obtained.

[0055] Preferably, in step (1), the aqueous phase liquid is heated to 80-85℃ (e.g., 81℃, 82℃, 83℃, 84℃ or 85℃) and kept at that temperature for 1-3 hours (e.g., 1.1 hours, 1.5 hours, 1.8 hours, 2 hours, 2.2 hours, 2.5 hours or 2.8 hours).

[0056] Preferably, in step (1), the oil phase liquid is heated to 75-85℃ (e.g., 76℃, 78℃, 80℃, 82℃ or 84℃, etc.) and kept at that temperature for 1-3 hours (e.g., 1.1 hours, 1.5 hours, 1.8 hours, 2 hours, 2.2 hours, 2.5 hours or 2.8 hours, etc.).

[0057] Preferably, the homogenization temperature in step (2) is 75-85°C, for example, it can be 76°C, 78°C, 80°C, 82°C or 84°C.

[0058] Preferably, the cooling in step (2) refers to cooling to 30-40℃, for example, 31℃, 33℃, 35℃, 37℃ or 39℃.

[0059] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0060] Compared with the prior art, the present invention has the following beneficial effects:

[0061] (1) The combination of Bifida Ferment Lysate, Yeast / Rice Fermentation Product Filtrate and Rose Water in the composition of the present invention with skin-rejuvenating, firming and anti-wrinkle effects has a synergistic effect, which can make the composition have multiple effects such as skin rejuvenation, anti-wrinkle and skin firming.

[0062] (2) The essence of the present invention, through the combination of the composition with skin-rejuvenating, firming and anti-wrinkle effects with moisturizers, emulsifiers and other components, can provide more nutrients and energy to skin cells, deeply moisturize the skin, and thus have excellent anti-wrinkle and skin-firming effects.

[0063] (3) The preparation method of the essence provided by the present invention is simple, more energy-saving and environmentally friendly, and suitable for large-scale production. Detailed Implementation

[0064] To further illustrate the technical means and effects of the present invention, the following describes the technical solution of the present invention in conjunction with preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0065] The following are some of the material and grade information involved in the embodiments and comparative examples:

[0066]

[0067] All other raw materials can be used as long as they are purchased from authorized distributors.

[0068] Example 1

[0069] This embodiment provides a composition with skin-rejuvenating, firming, and anti-wrinkle effects. The components of the composition with skin-rejuvenating, firming, and anti-wrinkle effects are, by weight, 20 parts of Bifida Ferment Lysate, 15 parts of Yeast / Rice Fermentation Product Filtrate, 8 parts of Rose Water, 1.5 parts of Hydroxypropyl Tetrahydropyranotriol, 3 parts of Tetrahydromethylpyrimidine Carboxylic Acid, and 1.5 parts of Micrococcus oxycarboxylate Extract.

[0070] Example 2

[0071] This embodiment provides a composition with skin-rejuvenating, firming and anti-wrinkle effects. The composition with skin-rejuvenating, firming and anti-wrinkle effects comprises, by weight, 25 parts of Bifida Ferment Lysate, 10 parts of Yeast / Rice Fermentation Product Filtrate, 10 parts of Rose Water, 1 part of Hydroxypropyl Tetrahydropyranotriol, 5 parts of Tetrahydromethylpyrimidine Carboxylic Acid, and 1 part of Microcystis oculiformis Extract.

[0072] Example 3

[0073] This embodiment provides a composition with skin-rejuvenating, firming and anti-wrinkle effects. The composition with skin-rejuvenating, firming and anti-wrinkle effects comprises, by weight, 15 parts of Bifida Ferment Lysate, 20 parts of Yeast / Rice Fermentation Product Filtrate, 5 parts of Rose Water, 2 parts of Hydroxypropyl Tetrahydropyranotriol, 2 parts of Tetrahydromethylpyrimidine Carboxylic Acid, and 2 parts of Microcystis oculiformis Extract.

[0074] Example 4

[0075] This embodiment provides a composition with skin-rejuvenating, firming and anti-wrinkle effects. The composition with skin-rejuvenating, firming and anti-wrinkle effects comprises, by weight, 20 parts of Bifida ferment filtrate, 15 parts of yeast / rice ferment filtrate and 8 parts of rose water.

[0076] Example 5

[0077] This embodiment provides a composition with skin-rejuvenating, firming and anti-wrinkle effects. The composition with skin-rejuvenating, firming and anti-wrinkle effects comprises, by weight, 20 parts of Bifida Ferment Lysate, 15 parts of Yeast / Rice Fermentation Product Filtrate, 8 parts of Rose Water, 2 parts of Hydroxypropyl Tetrahydropyranotriol and 4 parts of Tetrahydromethylpyrimidine Carboxylic Acid.

[0078] Example 6

[0079] This embodiment provides a composition with skin-rejuvenating, firming and anti-wrinkle effects. The composition with skin-rejuvenating, firming and anti-wrinkle effects comprises, by weight, 20 parts of Bifida ferment filtrate, 15 parts of yeast / rice ferment filtrate, 8 parts of rose water, 3 parts of hydroxypropyl tetrahydropyrantriol and 3 parts of Micrococcus iridis extract.

[0080] Example 7

[0081] This embodiment provides a composition with skin-rejuvenating, firming and anti-wrinkle effects. The composition with skin-rejuvenating, firming and anti-wrinkle effects comprises, by weight, 20 parts of Bifida ferment filtrate, 15 parts of yeast / rice ferment filtrate, 8 parts of rose water, 4 parts of tetrahydromethylpyrimidine carboxylic acid and 2 parts of Micrococcus iridis extract.

[0082] Comparative Example 1

[0083] This comparative example provides a composition with skin-rejuvenating, firming, and anti-wrinkle effects. The composition with skin-rejuvenating, firming, and anti-wrinkle effects does not include Bifida Ferment Lysate filtrate. The reduced amount of Bifida Ferment Lysate is allocated to the yeast / rice ferment filtrate and rose water in proportion to the amount of Bifida Ferment Lysate filtrate and rose water, while the remaining components and amounts remain unchanged.

[0084] Comparative Example 2

[0085] This comparative example provides a composition with skin-rejuvenating, firming, and anti-wrinkle effects. The composition with skin-rejuvenating, firming, and anti-wrinkle effects does not include yeast / rice ferment filtrate. The reduced amount of yeast / rice ferment filtrate is allocated to the Bifida ferment filtrate and rose water in a proportionate ratio, while the remaining components and amounts remain unchanged.

[0086] Comparative Example 3

[0087] This comparative example provides a composition with skin-rejuvenating, firming, and anti-wrinkle effects. The composition with skin-rejuvenating, firming, and anti-wrinkle effects does not include rose water. The reduced amount of rose water is allocated to the yeast / rice ferment filtrate and the bifida ferment filtrate in proportion to the amount of rose water. The remaining components and their amounts remain unchanged.

[0088] Application Examples 1-3

[0089] This application example provides three serums with the following formulas. The unit of measurement for each component in the table is "parts".

[0090]

[0091]

[0092] The composite ceramide is composed of ceramide 2, ceramide 3, ceramide 6 and ceramide 7 in a mass ratio of 1:1:3:1.

[0093] Its preparation method is as follows:

[0094] (1) After stirring water and humectant and heating to 80°C, keep warm for 2 hours to obtain an aqueous phase liquid;

[0095] (2) After stirring the emulsifier and thickener and heating to 80°C and holding for 2 hours, an oil phase liquid is obtained;

[0096] (2) After the heat preservation is completed, the aqueous phase liquid and the oil phase liquid are mixed and homogenized at 80°C. After cooling to 35°C, the composition with skin-rejuvenating, firming and anti-wrinkle effects is added. After homogenization, the essence is obtained.

[0097] Application Example 4-7

[0098] This application example provides four types of serums. The only difference between these serums and Application Example 1 is that the composition with skin-rejuvenating, firming, and anti-wrinkle effects in Example 1 is replaced in equal amounts with the compositions with skin-rejuvenating, firming, and anti-wrinkle effects in Examples 4-7. The remaining components and dosages are the same as in Application Example 1, and the preparation method is the same as in Application Example 1.

[0099] Application Example 8

[0100] This application example provides an essence, which differs from Application Example 1 only in that the moisturizer does not include compound ceramides, and the reduced amount is allocated to the polysaccharides and peptides in proportion. The remaining components and amounts are consistent with Application Example 1, and the preparation method is the same as that of Application Example 1.

[0101] Application Example 9

[0102] This application example provides an essence, which differs from Application Example 1 only in that the moisturizer does not contain peptides. The reduced amount of peptides is allocated to the complex ceramide and mucopolysaccharide in proportion to the amount of peptides. The remaining components and amounts are the same as in Application Example 1, and the preparation method is the same as in Application Example 1.

[0103] Application Example 10

[0104] This application example provides an essence, which differs from Application Example 1 only in that the moisturizer does not include mucopolysaccharides. The reduced amount is allocated to the compound ceramide and peptides in proportion, while the remaining components and amounts are consistent with Application Example 1. The preparation method is the same as in Application Example 1.

[0105] Comparative Application Examples 1-3

[0106] This application example provides three serums. The only difference between the serums and application example 1 is that the composition with skin-rejuvenating, firming and anti-wrinkle effects in example 1 is replaced in equal amounts with the compositions with skin-rejuvenating, firming and anti-wrinkle effects in comparative examples 1-3. The remaining components and dosages are the same as in application example 1, and the preparation method is the same as in application example 1.

[0107] Comparative Application Example 4

[0108] This application example provides a serum, which differs from Application Example 1 only in that it does not include the composition with skin-rejuvenating, firming and anti-wrinkle effects of Example 1. The reduction in its amount is made up by water, while the remaining components and amounts are consistent with Application Example 1. The preparation method is the same as that of Application Example 1.

[0109] Test Example 1: Antioxidant Efficacy Study – DPPH Free Radical Scavenging Experiment

[0110] Test samples: Samples provided in Application Examples 1-10 and Comparative Application Examples 1-4;

[0111] Test method:

[0112] Accurately weigh 47.7 mg of DPPH reagent and dilute to a 100 ml amber volumetric flask. Take 5 ml of this flask and dilute to a 50 ml amber volumetric flask with anhydrous ethanol to obtain the DPPH test solution (47.7 mg / L). Transfer 2 mL of the DPPH test solution to each of the following samples: 0.1 mL of the samples provided in Application Examples 1-10 and Comparative Application Examples 1-4, as shown in the table below. Add an appropriate amount of anhydrous ethanol to ensure equal total mass. Mix thoroughly and let stand in the dark for 30 min. Using anhydrous ethanol as a blank control, measure the absorbance at 517 nm. Vitamin C is used as a control. Calculate the DPPH scavenging rate of each extract using the following formula:

[0113] DPPH clearance rate (%) = [1 - (As - Ar) / A0] × 100%

[0114] Where: As—absorbance of the reaction system of extract and DPPH; Ar—absorbance of extract-anhydrous ethanol; A0—absorbance of blank control anhydrous ethanol.

[0115]

[0116] The DPPH free radical scavenging rate reflects an antioxidant capacity to some extent; a higher scavenging rate indicates better antioxidant activity and thus better anti-aging effects. Therefore, the anti-aging effect of a serum can be judged by studying its ability to scavenge DPPH free radicals. Vitamin C is a recognized high-efficiency antioxidant in the cosmetics industry. This experiment used Vitamin C as a control to examine its antioxidant capacity. The test results are shown in Table 1.

[0117] Table 1

[0118]

[0119]

[0120] As shown in Table 1, compared with Comparative Application Example 4, the serums of Application Examples 1-3 all showed better DPPH removal effects, with removal rates all above 88%. However, the DPPH removal effect of Application Examples 4-7 decreased. This indicates that the addition of hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, and *Microcystis globulus* extract to the composition can enhance the antioxidant effect of the serum. Furthermore, the simultaneous presence of these three components can better improve the anti-wrinkle and firming effects. The decreased DPPH removal effect in Comparative Application Examples 1-3 indicates that the three components—Bifida Ferment Lysate, Yeast / Rice Fermentation Product Filtrate, and Rose Water—mutually promote each other in terms of antioxidant effects, exhibiting a synergistic effect.

[0121] Test Example 2: Wrinkle Reduction and Skin Firming Test

[0122] The skin-firming effect of the serums prepared in corresponding use cases 1-10 and comparative application examples 1-4 was evaluated:

[0123] 140 participants aged 20-45 were randomly selected and divided into 14 groups of 10 each. The 15 groups used the serums prepared in Examples 1-10 and Control Examples 1-4 (twice daily, morning and evening) for 30 days. Participants were prohibited from using any other skin-maintaining products or cosmetics that might affect the results during the trial, and their original dietary habits remained unchanged.

[0124] Skin condition was tested on day 0 and day 30 using a Cutometer MPA580 skin elastometer. The test site was the cheekbone area. Measurements were taken three times on the same area, and the average value was recorded. The test parameter was R² (the ratio of skin rebound Ua without negative pressure to the maximum stretch Uf under negative pressure). The closer R² is to 1, the better the skin elasticity. The average change in R² = R² value on day 30 - R² value on day 0, and the average rate of change in R² = average change in R² / R² value on day 0. The specific skin elasticity test results are shown in Table 2.

[0125] Table 2

[0126]

[0127]

[0128] As shown in Table 2, the essence involved in this invention has a good skin-tightening effect. The three components, namely, Bifida Ferment Lysate, Yeast / Rice Ferment Lysate, and Rose Water, promote each other in this effect and have an unexpected synergistic effect. Furthermore, the addition of hydroxypropyltetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, and Micrococcus micranthum extract can further enhance the skin-tightening effect of the composition.

[0129] Test Example 3: VISIA Skin Analysis and Evaluation Experiment

[0130] The VISIA skin image analyzer employs multispectral imaging technology, using a 12-megapixel camera to capture images from three angles: front, left, and right. It images three times under three light sources: standard white light, cross-sectional polarized light, and ultraviolet light. Therefore, the VISIA facial image analysis system can systematically analyze the pigmentation, texture, pores, wrinkles, ultraviolet spots, stable pigmentation, red areas, and purplish-red areas of the subject's facial skin, providing precise and quantitative analytical data. By recording the pigmentation, wrinkles, and texture of the subject's face before and after the test, the absolute score represents the area and intensity of skin characteristic detection values, used to observe changes in skin properties. Therefore, the data obtained from the absolute score (the average of the test results from the front, left, and right sides) is selected as the analytical standard and data statistical basis. All data for each subject are entered into a computer, the measurement results are compared, and statistical analysis is performed to analyze the subject's skin characteristics and various indicator data. The testing instrument used in the experiment is the VISIA facial image analysis system (CANFIELD Technologies, USA).

[0131] The volunteers and test case 2 were from the same group of volunteers. They used the serums prepared in Application Examples 1-10 and Control Examples 1-4, respectively. Participants were required to use the products continuously, applying them twice daily (morning and evening) for 28 consecutive days. Testing was conducted on day 0 (D0), day 14 (D14), and day 28 (D28). Participants were prohibited from using any other skin-elasticity-maintaining products or cosmetics that might affect the results during the trial period, and their original dietary habits remained unchanged. Testing of the same participant was performed by the same measurement personnel.

[0132] The specific testing method is as follows:

[0133] After thoroughly cleansing their faces with a standardized facial cleanser, subjects gently patted their faces dry with a tissue. In a room with a temperature of 22℃±2℃ and a relative humidity of 40±2%, subjects waited 15 minutes before having their faces photographed using a VISIA facial image analyzer. The analysis area should be kept as clear as possible, avoiding reflective areas and interference from hair. Images of the subjects' faces (including the front, left side, and right side) were taken, and the absolute scores were statistically analyzed. Data on facial texture and wrinkles were recorded. The experimental results are shown in Table 3.

[0134] Table 3

[0135]

[0136]

[0137] As shown in Table 3, compared with Comparative Application Example 4, the serums in Application Examples 1-3 showed significant improvement in wrinkles and skin texture in the subjects. This indicates that the serum provided by the present invention, by adding a composition with skin-rejuvenating, firming, and anti-wrinkle effects, can achieve good wrinkle removal and skin texture smoothing effects. However, the improvement effect in Application Examples 4-7 decreased, indicating that the addition of hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, and *Microcystis aurea* extract can enhance the wrinkle removal and firming effects of the serum. Furthermore, the simultaneous presence of hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, and *Microcystis aurea* extract can better improve the anti-wrinkle and firming effects. In Application Examples 8-10, the choice of moisturizer also affected the anti-wrinkle and firming effects of the serum. The decrease in improvement effect compared with Application Examples 1-3 indicates that the three components—Bifida Ferment Lysate, Yeast / Rice Fermentation Product Filtrate, and Rose Water—mutually promote each other in improving wrinkles and skin texture, exhibiting a synergistic effect.

[0138] Test Example 4: Safety Evaluation Experiment

[0139] The specific method was as follows: Forty women aged 18-35 years with no history of allergies were selected. Each subject used samples prepared as described in Examples 1-10. After cleaning the subject's back, the patch applicator containing the sample was applied to the selected location on the back using non-irritating adhesive tape. After application, the patch was gently pressed with the fingers to ensure even application to the skin, and left on for 48 hours. During these 48 hours, the subject kept the patch area dry and avoided strenuous exercise, scratching, and prolonged sun exposure. After 48 hours, the applicator was removed and marked. After 30 minutes, once the indentation disappeared, the sample was evaluated under sufficient light.

[0140] The grading criteria for adverse skin reactions are shown in Table 4:

[0141] Table 4

[0142]

[0143] The results are shown in Table 5:

[0144] Table 5

[0145]

[0146]

[0147] As shown in Table 5, no adverse skin reactions were observed in the volunteers after the spot tester was removed. This indicates that the raw materials used in the essence of this invention are safe and non-irritating, have no adverse reactions on the human body, and are highly safe.

[0148] The applicant declares that this invention illustrates the composition with skin-rejuvenating, firming, and anti-wrinkle effects and its application through the above embodiments. However, this invention is not limited to the above embodiments, meaning that this invention does not necessarily rely on the above embodiments for implementation. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of the raw materials in the product, addition of auxiliary components, and selection of specific methods, etc., all fall within the protection and disclosure scope of this invention.

[0149] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0150] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable way without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. An essence, characterized in that, The essence is composed of 1-15 parts of a composition with skin-rejuvenating, firming and anti-wrinkle effects, 1-15 parts of a moisturizer, 1-10 parts of an emulsifier, 0.1-2 parts of a thickener and 45-90 parts of water. The composition having skin-rejuvenating, firming, and anti-wrinkle effects comprises, by weight, 15-25 parts of Bifida Ferment Lysate, 5-20 parts of Yeast / Rice Fermentation Product Filtrate, 5-10 parts of Rose Water, 1-2 parts of Hydroxypropyl Tetrahydropyranotriol, 1-5 parts of Tetrahydromethylpyrimidine Carboxylic Acid, and 0.1-2 parts of Micrococcus globulus Extract; the moisturizer is composed of polysaccharides, polypeptides, and complex ceramides in a mass ratio of (0.5-5):(0.5-10):(1-5). The polypeptide is ZPC® Creasend053S anti-aging complex peptide. The composite ceramides include ceramide 2, ceramide 3, ceramide 6 and ceramide 7; The emulsifiers include polyglycerol esters and PEG / PPG-14 / 7 dimethyl ether.

2. The essence as described in claim 1, characterized in that, The polyglycerol esters include polyglycerol fatty acid esters and / or polyglycerol ricinoleate.

3. The serum as described in claim 1, characterized in that, The thickener includes any one or a combination of at least two of xanthan gum, guar gum, hydroxyethyl cellulose, or sodium acrylate / sodium acryloyl dimethyl taurate copolymer.

4. A method for preparing an essence as described in any one of claims 1-3, characterized in that, The preparation method includes: (1) Aqueous phase liquid is obtained by stirring water and humectant, heating and keeping warm; oil phase liquid is obtained by stirring emulsifier and thickener, heating and keeping warm; (2) After the heat preservation is completed, the aqueous phase liquid and the oil phase liquid are mixed and homogenized, cooled down, and a composition with skin-rejuvenating, firming and anti-wrinkle effects is added. After homogenization, the essence is obtained.

5. The preparation method according to claim 4, characterized in that, In step (1), the aqueous phase liquid is prepared by heating to 80-85℃ and holding for 1-3 hours.

6. The preparation method according to claim 4, characterized in that, In step (1), the oil phase liquid is prepared by heating to 75-85℃ and holding for 1-3 hours.

7. The preparation method according to claim 4, characterized in that, The homogenization temperature in step (2) is 75-85℃.

8. The preparation method according to claim 4, characterized in that, The cooling mentioned in step (2) refers to cooling down to 30-40℃.

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