A primer group, kit and method for identifying yunmang 3

The identification problem of Yunmang No. 3 was solved by PCR amplification and fluorescence capillary electrophoresis detection using primer sets and kits, achieving efficient and low-cost accurate identification and ensuring the authenticity of the variety and market protection.

CN119332009BActive Publication Date: 2026-07-14YUNNAN INST OF TROPICAL CROPS

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YUNNAN INST OF TROPICAL CROPS
Filing Date
2024-10-30
Publication Date
2026-07-14

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Abstract

The disclosure provides a primer group, a kit and a method for identifying Yunmang No. 3, and belongs to the field of germplasm resource identification. The primer group comprises six primer pairs, each primer pair comprising a forward primer and a reverse primer, the forward primer of the first primer pair, the reverse primer of the first primer pair, the forward primer of the sixth primer pair and the reverse primer of the sixth primer pair are sequentially shown as SEQ ID NO: 1 to SEQ ID NO: 12 in the sequence table. The primer group and the corresponding method provided in the embodiment can overcome the uncertainty of variety identification based on the external morphological characteristics of mango, accurately identify the new mango variety Yunmang No. 3, and are simple to operate, low in cost, high in efficiency and intuitive and reliable in result. The disclosure provides a scientific technical support for the protection of the new mango variety Yunmang No. 3, and is conducive to the popularization and application of the mango variety.
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