An observation device based on toxicity test and method thereof

Abstract

The application discloses an observation device based on toxicity test and a method thereof, and belongs to the technical field of observation devices, which comprises a base station; a plurality of long strip-shaped observation boxes are installed on the base station; a detection mechanism for detecting the weight and movement ability of mice is installed on the observation boxes; the detection mechanism comprises a floating feeding table, an adjusting support assembly and an electronic scale; one end of the observation box is provided with the floating feeding table for feeding, and the other end of the observation box is provided with the floating feeding table for feeding water; the lower side of the two ends of the observation box is provided with the adjusting support assembly for adjusting the height of the floating feeding table; the top of the adjusting support assembly is provided with the electronic scale for weighing the floating feeding table; the lower side of the observation box on the base station is provided with a collecting mechanism for collecting and storing the mouse excrement in the plurality of observation boxes according to the defecation time and sequence. In the above manner, the excrement of the mice is automatically collected and stored in real time according to time during the observation process.

Description

Technical Field

[0001] This invention relates to the field of observation device technology, and more specifically to an observation device and method based on toxicity testing. Background Technology

[0002] The basic procedure for animal toxicity experiments is to inject toxic drugs into mice by injection, feeding, or gavage, and then place the mice in an observation device. The experimenters observe, record, and analyze the mice's physical characteristics, such as weight, mobility, drug residue in feces, overall mortality rate, and the corresponding drug injection dosage.

[0003] For example, Chinese patent CN219330352U discloses a mouse observation box and its experimental equipment. The observation box and experimental equipment are equipped with an observation window, a feces storage mechanism and a cleaning mechanism. The mouse condition is observed through the observation window, and the mouse feces are swept into the feces storage mechanism for collection and storage through the cleaning mechanism.

[0004] However, the timing and frequency of mouse defecation are difficult to control, and if the feces are not preserved in time after excretion, they will easily dry out, or multiple excrements will accumulate, making it difficult to record the corresponding excretion time and affecting the accuracy of the test. In addition, when the device detects the weight of mice, the mice need to be taken out of the observation box for measurement, which is inconvenient to operate.

[0005] Based on this, the present invention designs an observation device and method based on toxicity testing to solve the above problems. Summary of the Invention

[0006] In view of the above-mentioned shortcomings of the existing technology, the present invention provides an observation device and method based on toxicity testing.

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] An observation device based on toxicity testing, comprising a base;

[0009] Multiple elongated observation boxes are mounted on the base; each observation box is equipped with a detection mechanism for detecting the mouse's weight and motor ability.

[0010] The testing mechanism includes a floating feeding platform, an adjustable support assembly, and an electronic scale; a floating feeding platform for feeding is installed at one end of the observation box, and a floating feeding platform for watering is installed at the other end of the observation box; adjustable support assemblies for adjusting the height of the floating feeding platforms are installed on the lower sides of both ends of the observation box; an electronic scale for weighing the floating feeding platform is installed on the top of the adjustable support assembly.

[0011] A collection mechanism is installed on the base, aligned with the lower side of the observation box, for collecting and storing mouse feces from multiple observation boxes according to defecation time and order.

[0012] Furthermore, the observation box includes an observation box body, a locking component, and a blocking component. The observation box body is mounted on a base, the locking component is mounted on the observation box body, and the blocking component is mounted on the observation box body.

[0013] Furthermore, the main body of the observation box includes a first support frame, a first support platform, a box body, side covers, a feeding port, a middle cover, and a bottom structure. The two ends of the box body are respectively fixedly mounted on the upper side of a first support platform, the first support platform is fixedly mounted on the upper side of a first support frame, and the first support frame is fixedly mounted on the upper side of a base. A middle cover is rotatably mounted on the top center of the box body, and two side covers are symmetrically arranged at both ends of the box body. One side of each side cover is rotatably connected to the top of the box body, and a feeding port is provided on the side of each side cover. A bottom structure is installed at the bottom of the box body. Floating feeding platforms are symmetrically installed at both ends of the bottom of the box body. The box body, side covers, middle cover, bottom structure, and floating feeding platforms constitute a movement space for the mouse. Locking components for locking the side covers are installed on the box body and side covers, and a locking component for locking the middle cover is installed between the box body and the middle cover.

[0014] Furthermore, the bottom structure includes a collection hopper and round rods, with multiple crisscrossing round rods fixedly installed at the bottom of the box; a collection hopper that is larger at the top and smaller at the bottom is also fixedly installed at the bottom of the box.

[0015] Furthermore, the locking assembly includes a locking hole and a locking pin. The locking hole is provided on the side of the housing, side cover and middle cover away from the hinge point; the locking hole on the housing is aligned with the locking holes on the side cover and middle cover; the locking pin is inserted into the locking hole.

[0016] Furthermore, the guard assembly includes a mounting block, insert rods, and slots. Insert rods are evenly and uniformly fixed on the mounting block, and through slots are symmetrically opened on the side of the housing, with the slots located between the side cover and the middle cover; the insert rods are inserted into the slots.

[0017] Furthermore, the floating feeding platform includes sliding rods, limiting blocks, support plates, feeding troughs, and baffles. The support plate is located inside the box, and multiple sliding rods are symmetrically fixedly installed on the lower side of the support plate. The bottom of the sliding rods passes through the lower side of the box and is fixedly connected to the limiting blocks. The sliding rods are slidably connected to the box. A feeding trough is fixedly installed on the upper outer side of the support plate, and the feeding trough is aligned with the feeding port. A baffle is fixedly installed on the lower inner side of the support plate.

[0018] Furthermore, the adjusting support assembly includes a first motor, a screw, a movable sleeve, a guide rod, and a second support platform. The first motor is fixedly installed on the lower side of the first support frame. The output end of the first motor passes through the first support frame and the first support platform and is fixedly connected to the lower end of the screw. The lower side of the movable sleeve is sleeved over the screw, and the screw is threadedly connected to the movable sleeve through a threaded sleeve. The top of the movable sleeve is fixedly installed with the second support platform, and guide rods are symmetrically fixedly installed on the lower side of the second support platform. The guide rods are slidably connected to the first support platform. An electronic scale for weighing the support plate is fixedly installed on the upper side of the second support platform.

[0019] Furthermore, the collection mechanism includes a film, a feeding roller, a guide roller, an adjusting roller, a feed roller, a take-up roller, and a second support frame. The second support frame is symmetrically fixed on the base. From left to right, the second support frame is rotatably mounted with a feeding roller for driving the film unwinding, a guide roller for guiding and supporting the film, an adjusting roller for adjusting the film tension, a feed roller for driving the film to move uniformly to the right, and a take-up roller for winding the film. The film between the feed roller and the take-up roller is aligned with the lower side of multiple collection hoppers.

[0020] To better achieve the objectives of this invention, this invention also provides a testing method based on an observation device for toxicity testing, comprising the following steps:

[0021] Step 1: Select multiple mice and divide them into three groups: a, b, and c; administer the drugs to the mice orally or by gavage during the experiment;

[0022] Step 2: Administer the drug to mice in group a, and increase the dosage after observing the mice recovering. Repeat this process multiple times until the dosage in group a mice is close to the lethal dose range to cultivate drug resistance in the mice. Mice in groups b and c are not given the drug initially. Place the mice in the main body of the observation box, close the main body of the observation box and lock it using the locking component, and monitor the vital signs of the mice inside the observation box.

[0023] Step 3: When the mouse leaves one end of the floating feeding platform, zero the electronic scale under the floating feeding platform. When the mouse returns to the floating feeding platform, weigh the mouse on the floating feeding platform using the electronic scale.

[0024] Step 4: After administering the medication to the mice, adjust the floating feeding platforms at both ends to a lower position by adjusting the support components, so that the mice can move normally to the floating feeding platforms to eat. During the subsequent feeding process, gradually raise the height of the floating feeding platforms by adjusting the support components. If the mice cannot or have difficulty moving to the floating feeding platforms after raising the height, the height of the floating feeding platforms can be lowered. Continue until the mice are able to climb normally to the floating feeding platforms at a higher position. At this point, the mice are considered to have recovered their mobility.

[0025] Step 5: While performing steps 1, 2, and 3, mouse feces are collected and preserved in real time using a collection device. This device also records the time of each mouse defecation, eliminating the need for researchers to collect and record feces in real time. This makes recording more accurate and collection and preservation more timely, maximizing the accuracy of fecal samples. By analyzing the components of the mouse fecal samples, the presence and amount of toxic drug residues in the feces can be determined, as well as the changes in the amount of toxic drug residues in the mouse feces over time.

[0026] Step Six: Repeat steps Two, Three, Four, and Five until the dosage of the drug in group a mice is close to the lethal dose range. At the same time, increase the dosage of the drug to group a and group b mice. Observe the condition and mortality rate of group a and group b mice and draw the corresponding experimental conclusions.

[0027] Step 7: All surviving mice are assigned to group a, and mice from group c are selected to be assigned to group b. After a period of feeding and recovery, the dosage of the drug is increased again for mice in groups a and b. The condition and mortality rate of mice in groups a and b are observed, and the corresponding experimental conclusions are drawn. This operation is repeated until the mortality rate of one group of mice in groups a and b is greater than 90%, at which point the experiment ends.

[0028] Compared with the prior art, the beneficial effects of this invention are as follows: 1. By setting the floating feeding platforms for feeding food and water at the two ends of the main body of the observation box, the mice are prevented from staying at the same end for a long time; when the mice leave the floating feeding platform at one end, the electronic scale under the floating feeding platform is zeroed; when the mice return to the floating feeding platform, the weight of the mice on the floating feeding platform is measured by the electronic scale.

[0029] 2. After administering the drug to the mice, adjust the floating feeding platforms at both ends to a lower position by adjusting the support components, so that the mice can move normally to the floating feeding platforms to eat. During the subsequent feeding process, gradually raise the height of the floating feeding platforms by adjusting the support components. If the mice cannot or have difficulty moving to the floating feeding platforms after raising the height, the height of the floating feeding platforms can be lowered. Continue until the mice are able to climb normally to the floating feeding platforms at a higher position. At this point, the mice are considered to have recovered their mobility.

[0030] 3. The design of the observation box body, locking components, and blocking components facilitates the removal of mice from the observation box body and prevents mice from escaping from the observation box body when being removed.

[0031] 4. Mouse feces are collected and stored in real time through a collection device, which also records the time of each defecation. This eliminates the need for researchers to collect and record mouse feces in real time, making the records more accurate and the collection and storage more timely, thus maximizing the accuracy of the fecal samples. By analyzing the components of mouse fecal samples, it is possible to determine whether there are toxic drug residues in the mouse feces and the amount of residues, as well as how the amount of toxic drug residues in mouse feces changes over time. Attached Figure Description

[0032] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without any creative effort.

[0033] Figure 1 A three-dimensional observation device based on toxicity testing according to the present invention. Figure 1 ;

[0034] Figure 2 This is a front view of an observation device based on toxicity testing according to the present invention;

[0035] Figure 3 This is a schematic diagram of the first support platform and its connecting structure;

[0036] Figure 4 This is a schematic diagram of the box and its connection structure;

[0037] Figure 5 This is a schematic diagram of the side cover and its connection structure;

[0038] Figure 6 This is a schematic diagram of the feeding trough and its connection structure.

[0039] Figure 7 This is a schematic diagram of the first support platform and its connecting structure;

[0040] Figure 8 This is a schematic diagram of the second support frame and its connecting structure;

[0041] Figure 9 This is a schematic diagram of the thin film and its connection structure.

[0042] The labels in the diagram represent:

[0043] 1. Base; 2. Observation box; 21. Observation box body; 211. First support frame; 212. First support platform; 213. Box body; 214. Side cover; 215. Feeding port; 216. Middle cover; 217. Collection hopper; 218. Round rod; 22. Locking assembly; 221. Locking hole; 222. Locking pin; 23. Barrier assembly; 231. Mounting block; 232. Insert rod; 3. Detection mechanism; 31. Floating feeding platform; 31 1. Slide bar; 312. Limiting block; 313. Support plate; 314. Feeding trough; 315. Baffle; 32. Adjusting support assembly; 321. First motor; 322. Screw; 323. Moving sleeve; 324. Guide rod; 325. Second support platform; 33. Electronic scale; 4. Collection mechanism; 41. Film; 42. Feeding roller; 43. Guide roller; 44. Adjusting roller; 45. Feeding roller; 46. Rewinding roller; 47. Second support frame. Detailed Implementation

[0044] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0045] The terms "left," "right," "front," "back," "up," and "down" used in the following description refer to the orientation from the perspective of the front view.

[0046] Example 1: In some embodiments, please refer to the accompanying drawings. Figures 1-3 An observation device based on toxicity testing includes a base 1;

[0047] Multiple elongated observation boxes 2 are installed on the base 1; each observation box 2 is equipped with a detection mechanism 3 for detecting the weight and motor ability of mice.

[0048] The detection mechanism 3 includes a floating feeding platform 31, an adjusting support assembly 32, and an electronic scale 33; a floating feeding platform 31 for feeding is installed at one end of the observation box 2, and a floating feeding platform 31 for watering is installed at the other end of the observation box 2; adjusting support assemblies 32 for adjusting the height of the floating feeding platform 31 are installed on the lower sides of both ends of the observation box 2; an electronic scale 33 for weighing the floating feeding platform 31 is installed on the top of the adjusting support assembly 32.

[0049] A collection mechanism 4 is installed on the base 1, aligned with the lower side of the observation box 2, for collecting and storing mouse feces in multiple observation boxes 2 according to defecation time and order.

[0050] In this embodiment, when the toxicity test-based observation device is working normally, multiple sets of devices are set up, and multiple mice are selected and divided into three groups: a, b, and c. During the experiment, the mice are administered drugs orally or by gavage. The mice are placed in the main body 21 of the observation box, and after the main body 21 of the observation box is closed, it is locked by the locking component 22. The mice are then fed until the set time of the experiment ends.

[0051] During this process, by setting the floating feeding platform 31 for feeding and watering at both ends of the observation box body 21, the mice are prevented from staying at the same end for a long time. When the mice leave the floating feeding platform 31 at one end, the electronic scale 33 on the lower side of the floating feeding platform 31 is zeroed. When the mice return to the floating feeding platform 31, the weight of the mice on the floating feeding platform 31 is measured by the electronic scale 33.

[0052] After administering the drug to the mice, the floating feeding platforms 31 at both ends were adjusted to a lower position by adjusting the support component 32, so that the mice could move normally onto the floating feeding platforms 31 to eat. During the subsequent feeding process, the height of the floating feeding platforms 31 was gradually increased by adjusting the support component 32. If the mice could not or had difficulty moving onto the floating feeding platforms 31 after increasing the height, the height of the floating feeding platforms 31 could be reduced. This process continued until the mice were able to climb normally onto the floating feeding platforms 31 at a higher position, at which point the mice were considered to have recovered their mobility.

[0053] The design of the observation box body 21, locking component 22 and blocking component 23 facilitates the removal of mice from the observation box body 21 and prevents mice from escaping from the observation box body 21 when they are removed.

[0054] Mouse feces are collected and stored in real time through collection device 4. Simultaneously, the collection device 4 records the time of each mouse defecation, eliminating the need for researchers to collect and record defecation times in real time. This makes recording more accurate, collection and storage more timely, and maximizes the accuracy of fecal samples. By analyzing the components of mouse fecal samples, the presence and amount of toxic drug residues in the feces can be determined, as well as the changes in the amount of toxic drug residues in the mouse feces over time.

[0055] During the initial preparation, mice in group A were given a smaller initial dose, and the dose was increased after the mice recovered. This process was repeated multiple times until the dose in group A approached the lethal dose range, thereby cultivating drug resistance in the mice. Mice in groups B and C were not given any drug initially.

[0056] When the drug dosage for group a mice is close to the lethal dose range, and the drug dosage is increased for both group a and group b mice; when the mortality rates of both group a and group b mice are low (below 20%), if the mortality rate of group a mice is greater than that of group b mice, then the drug administration to group a mice during the early feeding phase causes short-term irreversible damage to the mice; if the mortality rates of group a mice and group b mice are not significantly different, then the drug administration to group a mice during the early feeding phase does not cause short-term irreversible damage to the mice.

[0057] The surviving mice were then assigned to group a, and mice were selected from group c to be assigned to group b. After a period of feeding, the mice regained their mobility, ate normally, and their weight did not decrease significantly compared to the previous weighing, and there were no toxic drug residues in their feces. The mice were then removed to test other vital signs, and the drug dosage was increased again and administered to mice in groups a and b. This process was repeated until the mortality rate of one group of mice in groups a and b was greater than 90%, at which point the experiment was terminated.

[0058] During this process, the mortality rates of mice in groups a and b were continuously observed. If the mortality rates of mice in group a and group b were the same, then no significant drug resistance was observed in the early feeding of mice in group a during this experimental period. If the mortality rate of mice in group a was significantly lower than that of mice in group b (more than twice the difference), then mice in group a developed drug resistance during the early feeding stage. If the mortality rate of mice in group a was significantly higher than that of mice in group b (more than twice the difference), then the drug administration in the previous feeding stage caused short-term, irreversible damage to mice in group a.

[0059] Example 2: In some embodiments, such as Figures 1-9As shown, in a preferred embodiment of the present invention, the observation box 2 includes an observation box body 21, a locking component 22 and a blocking component 23. The observation box body 21 is mounted on the base 1, the locking component 22 is mounted on the observation box body 21, and the blocking component 23 is mounted on the observation box body 21.

[0060] The main body 21 of the observation box includes a first support frame 211, a first support platform 212, a box body 213, side covers 214, a feeding port 215, a middle cover 216, and a bottom structure. The two ends of the box body 213 are respectively fixedly mounted on the upper side of a first support platform 212. The first support platform 212 is fixedly mounted on the upper side of the first support frame 211, and the first support frame 211 is fixedly mounted on the upper side of the base 1. A middle cover 216 is rotatably mounted on the top center of the box body 213. Two side covers 214 are symmetrically arranged at both ends of the box body 213. One side of the side cover 214... The top of the box 213 is rotatably connected to the side cover 214, which has a feeding port 215 on its side. The bottom of the box 213 is equipped with a bottom structure. The floating feeding platform 31 is symmetrically installed at both ends of the bottom of the box 213. The box 213, the side cover 214, the middle cover 216, the bottom structure and the floating feeding platform 31 form a movement space for the mouse. The box 213 and the side cover 214 are equipped with locking components 22 for locking the side cover 214, and the box 213 and the middle cover 216 are equipped with locking components 22 for locking the middle cover 216.

[0061] The bottom structure includes a collection hopper 217 and round rods 218. Multiple intersecting round rods 218 are fixedly installed at the bottom of the box body 213. A collection hopper 217, which is larger at the top and smaller at the bottom, is fixedly installed at the bottom of the box body 213.

[0062] The locking assembly 22 includes a locking hole 221 and a locking pin 222. The locking hole 221 is provided on the side of the housing 213, the side cover 214 and the middle cover 216 away from the hinge point; the locking hole 221 on the housing 213 is aligned with the locking hole 221 on the side cover 214 and the middle cover 216; the locking pin 222 is inserted into the locking hole 221.

[0063] The guard assembly 23 includes a mounting block 231, a rod 232, and a slot. The rod 232 is evenly fixedly mounted on the mounting block 231 at equal intervals. The side of the housing 213 is symmetrically provided with through slots, which are located between the side cover 214 and the middle cover 216. The rod 232 is inserted into the slot.

[0064] The floating feeding platform 31 includes a sliding rod 311, a limiting block 312, a support plate 313, a feeding trough 314, and a baffle 315. The support plate 313 is located inside the housing 213. Multiple sliding rods 311 are symmetrically fixedly installed on the lower side of the support plate 313. The bottom of the sliding rods 311 passes through the lower side of the housing 213 and is fixedly connected to the limiting block 312. The sliding rods 311 are slidably connected to the housing 213. The feeding trough 314 is fixedly installed on the upper outer side of the support plate 313 and is aligned with the feeding port 215. The baffle 315 is fixedly installed on the lower inner side of the support plate 313.

[0065] The adjusting support assembly 32 includes a first motor 321, a screw 322, a movable sleeve 323, a guide rod 324, and a second support platform 325. The first motor 321 is fixedly installed on the lower side of the first support frame 211. The output end of the first motor 321 passes through the first support frame 211 and the first support platform 212 and is fixedly connected to the lower end of the screw 322. The lower side of the movable sleeve 323 is sleeved on the outside of the screw 322, and the screw 322 is threadedly connected to the movable sleeve 323 through a threaded sleeve. The second support platform 325 is fixedly installed on the top of the movable sleeve 323. The guide rods 324 are symmetrically fixedly installed on the lower side of the second support platform 325. The guide rods 324 are slidably connected to the first support platform 212. An electronic scale 33 for weighing the support plate 313 is fixedly installed on the upper side of the second support platform 325.

[0066] The collection mechanism 4 includes a film 41, a feeding roller 42, a guide roller 43, an adjusting roller 44, a feed roller 45, a take-up roller 46, and a second support frame 47. The second support frame 47 is symmetrically fixed on the base 1. The second support frame 47 is rotatably mounted from left to right with the following components: a feeding roller 42 for unwinding the film 41, a guide roller 43 for guiding and supporting the film 41, an adjusting roller 44 for adjusting the tension of the film 41, a feed roller 45 for moving the film 41 uniformly to the right, and a take-up roller 46 for winding the film 41. The film 41 between the feed roller 45 and the take-up roller 46 is aligned with the lower side of multiple collection hoppers 217.

[0067] The feeding roller 42, feed roller 45, and take-up roller 46 are all driven by motors, specifically servo motors or geared motors. The feed roller 45 includes a drive roller and a driven roller, which are rotatably mounted on the second support frame 47. The drive roller and the driven roller clamp the film 41 on the upper and lower sides. The motor drives the drive roller to rotate, and the drive roller conveys the film 41 at a uniform speed through the cooperation of the driven roller.

[0068] In this embodiment, when the observation box 2, the detection mechanism 3, and the collection mechanism 4 are working normally, the locking pin 222 locks the side cover 214 and the middle cover 216 to the box body 213 through the locking hole 221. Before removing the mouse from the box body 213, the mouse's location can be observed. By inserting the insertion rod 232 into the slot, the box body 213 is separated by the insertion rod 232, preventing the mouse from escaping to other areas inside the box body 213 when it is removed. Then, the locking pin 222 corresponding to the area where the mouse is located is pulled out, so that the locking pin 222 no longer obstructs the movement of the upper side cover 214 or the middle cover 216. The side cover 214 or the middle cover 216 is flipped open, and the mouse is taken out or put in. Then, the insertion rod 232 is pulled out so that it does not affect the movement of the mouse inside the box body 213.

[0069] The first motor 321 drives the screw 322 to rotate. The rotation of the screw 322 drives the moving sleeve 323 and the second support platform 325 to move. The moving sleeve 323 and the second support platform 325 move vertically under the limiting action of the guide rod 324, thereby pushing the support plate 313 to move through the electronic scale 33. The support plate 313 moves vertically within the box 213 under the limiting action of the slide rod 311 and the limiting block 312, thereby realizing the adjustment of the position of the support plate 313. The baffle 315 prevents the mouse from escaping from under the support plate 313.

[0070] Food or water is fed into the feeding trough 314 through the feeding port 215. By setting the feeding troughs 314 for food and water at opposite ends of the box 213, the mice are prevented from staying in one position. After the mice leave the support plate 313, the electronic scale 33 is zeroed. After the mice return to the support plate 313, the weight of the mice is measured by the electronic scale 33. Thus, the weight of the mice can be measured before they eat without removing them from the box 213.

[0071] During the experiment, the take-up roller 46, in coordination with the feed roller 42, guide roller 43, adjusting roller 44, and feed roller 45, uniformly winds up the film 41 at a low speed, keeping the linear velocity of the film 41 constant. The feces produced by the experimental mice fall through the crisscrossing circular rods 218 and, guided by the collection hopper 217, land on the upper side of the film 41 between the feed roller 45 and the take-up roller 46. The take-up roller 46 winds up the film 41, simultaneously winding up the mouse feces on it. The film 41 collects the mouse feces promptly, and the wound film 41 encapsulates the feces, thus preserving the film 41. By uniformly winding up the film 41, the defecation time can be estimated based on the location of the feces. Therefore, when analyzing the fecal composition, the toxic drug residues in the feces and the changes in toxic drug residues in the feces over time can be obtained.

[0072] Example 3: In some embodiments, such as Figures 1-9 As shown, as a preferred embodiment of the present invention, a test method for an observation device based on toxicity testing includes the following steps:

[0073] Step 1: Select multiple mice and divide them into three groups: a, b, and c; administer the drugs to the mice orally or by gavage during the experiment;

[0074] Step 2: Administer the drug to group a mice, and increase the dosage after observing the mice recovering. Repeat this process multiple times until the dosage of group a mice is close to the lethal dose range to cultivate drug resistance in the mice. Group b and group c mice are not given the drug initially. Place the mice in the observation box body 21, close the observation box body 21 and lock it using the locking component 22, and monitor the vital signs of the mice inside the observation box body 21.

[0075] Step 3: When the mouse leaves the floating feeding platform 31 at one end, the electronic scale 33 under the floating feeding platform 31 is zeroed. When the mouse returns to the floating feeding platform 31, the weight of the mouse on the floating feeding platform 31 is measured by the electronic scale 33.

[0076] Step 4: After administering the medication to the mice, adjust the floating feeding platforms 31 at both ends to a lower position by adjusting the support component 32, so that the mice can move normally onto the floating feeding platforms 31 to eat. During the subsequent feeding process, gradually raise the height of the floating feeding platforms 31 by adjusting the support component 32. If the mice cannot or have difficulty moving onto the floating feeding platforms 31 after raising the height, the height of the floating feeding platforms 31 can be lowered. Continue until the mice are able to climb normally to the floating feeding platforms 31 at a higher position. At this point, the mice are considered to have recovered their mobility.

[0077] Step 5: While performing steps 1, 2, and 3, mouse feces are collected and stored in real time using collection device 4. Simultaneously, the collection device 4 records the time of each mouse defecation, eliminating the need for researchers to collect and record defecation times in real time. This makes recording more accurate, collection and storage more timely, and maximizes the accuracy of fecal samples. By analyzing the components of mouse fecal samples, the presence and amount of toxic drug residues in the feces can be determined, as well as the changes in the amount of toxic drug residues in mouse feces over time.

[0078] Step Six: Repeat steps Two, Three, Four, and Five until the dosage of the drug in group a mice is close to the lethal dose range. At the same time, increase the dosage of the drug to group a and group b mice. Observe the condition and mortality rate of group a and group b mice and draw the corresponding experimental conclusions.

[0079] Step 7: All surviving mice are assigned to group a, and mice from group c are selected to be assigned to group b. After a period of feeding and recovery, the dosage of the drug is increased again for mice in groups a and b. The condition and mortality rate of mice in groups a and b are observed, and the corresponding experimental conclusions are drawn. This operation is repeated until the mortality rate of one group of mice in groups a and b is greater than 90%, at which point the experiment ends.

[0080] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An observation device based on toxicity testing, comprising a base (1), characterized in that: Multiple elongated observation boxes (2) are installed on the base (1); the observation boxes (2) are equipped with a detection mechanism (3) for detecting the weight and motor ability of mice. The testing mechanism (3) includes a floating feeding platform (31), an adjusting support assembly (32), and an electronic scale (33); a floating feeding platform (31) for feeding is installed at one end of the observation box (2), and a floating feeding platform (31) for water feeding is installed at the other end of the observation box (2); an adjusting support assembly (32) for adjusting the height of the floating feeding platform (31) is installed on the lower side of both ends of the observation box (2); an electronic scale (33) for weighing the floating feeding platform (31) is installed on the top of the adjusting support assembly (32). A collection mechanism (4) is installed on the base (1) facing the lower side of the observation box (2) for collecting and storing mouse feces in multiple observation boxes (2) according to the defecation time and order. The observation box (2) includes an observation box body (21), a locking component (22) and a blocking component (23). The observation box body (21) is mounted on the base (1), the locking component (22) is mounted on the observation box body (21), and the blocking component (23) is mounted on the observation box body (21). The main body (21) of the observation box includes a first support frame (211), a first support platform (212), a box body (213), side covers (214), a feeding port (215), a middle cover (216), and a bottom structure. The two ends of the box body (213) are respectively fixedly installed on the upper side of a first support platform (212), the first support platform (212) is fixedly installed on the upper side of the first support frame (211), and the first support frame (211) is fixedly installed on the upper side of the base (1). A middle cover (216) is rotatably installed in the middle of the top of the box body (213), and two side covers (214) are symmetrically arranged at both ends of the box body (213). One side of the side cover (214) The top of the box (213) is rotatably connected to the side cover (214), and a feeding port (215) is opened on the side of the side cover (214); a bottom structure is installed at the bottom of the box (213); a floating feeding platform (31) is symmetrically installed at both ends of the bottom of the box (213); the box (213), the side cover (214), the middle cover (216), the bottom structure and the floating feeding platform (31) constitute the movement space of the mouse; a locking component (22) for locking the side cover (214) is installed on the box (213) and the side cover (214), and a locking component (22) for locking the middle cover (216) is installed between the box (213) and the middle cover (216). The floating feeding platform (31) includes a sliding rod (311), a limiting block (312), a support plate (313), a feeding trough (314), and a baffle (315). The support plate (313) is located inside the box (213). Multiple sliding rods (311) are symmetrically fixedly installed on the lower side of the support plate (313). The bottom of the sliding rod (311) passes through the lower side of the box (213) and is fixedly connected to the limiting block (312). The sliding rod (311) is slidably connected to the box (213). The feeding trough (314) is fixedly installed on the upper side of the outer end of the support plate (313) and is aligned with the feeding port (215). The baffle (315) is fixedly installed on the lower side of the inner end of the support plate (313). The adjusting support assembly (32) includes a first motor (321), a screw (322), a movable sleeve (323), a guide rod (324), and a second support platform (325). The first motor (321) is fixedly installed on the lower side of the first support frame (211). The output end of the first motor (321) passes through the first support frame (211) and the first support platform (212) and is fixedly connected to the lower end of the screw (322). The lower side of the movable sleeve (323) is sleeved on the screw. Outside the rod (322), the screw (322) is threadedly connected to the movable sleeve rod (323) through a threaded sleeve; a second support platform (325) is fixedly installed on the top of the movable sleeve rod (323), and guide rods (324) are symmetrically fixedly installed on the lower side of the second support platform (325). The guide rods (324) are limited and slidably connected to the first support platform (212); an electronic scale (33) for weighing the support plate (313) is fixedly installed on the upper side of the second support platform (325).

2. The observation device based on toxicity testing according to claim 1, characterized in that, The bottom structure includes a collection hopper (217) and round rods (218). Multiple intersecting round rods (218) are fixedly installed at the bottom of the box (213); a collection hopper (217) with a larger top and a smaller bottom is fixedly installed at the bottom of the box (213).

3. The observation device based on toxicity testing according to claim 2, characterized in that, The locking assembly (22) includes a locking hole (221) and a locking pin (222). The locking hole (221) is provided on the side away from the hinge point of the housing (213), the side cover (214) and the middle cover (216); the locking hole (221) on the housing (213) is aligned with the locking hole (221) on the side cover (214) and the middle cover (216); the locking pin (222) is inserted into the locking hole (221).

4. The observation device based on toxicity testing according to claim 3, characterized in that, The blocking assembly (23) includes a mounting block (231), a plug rod (232) and a slot. The plug rod (232) is evenly fixed on the mounting block (231) at equal intervals. The side of the housing (213) is symmetrically provided with through slots, which are located between the side cover (214) and the middle cover (216). The plug rod (232) is inserted into the slot.

5. The observation device based on toxicity testing according to claim 4, characterized in that, The collection mechanism (4) includes a film (41), a feeding roller (42), a guide roller (43), an adjusting roller (44), a feed roller (45), a take-up roller (46), and a second support frame (47). The second support frame (47) is symmetrically fixed on the base (1). The second support frame (47) is rotatably mounted from left to right with the following components: a feeding roller (42) for driving the film (41) to unwind; a guide roller (43) for guiding and supporting the film (41); an adjusting roller (44) for adjusting the tension of the film (41); a feed roller (45) for driving the film (41) to move to the right at a uniform speed; and a take-up roller (46) for winding the film (41). The film (41) between the feed roller (45) and the take-up roller (46) is aligned with the lower side of multiple collection hoppers (217).

6. A test method for an observation device based on toxicity testing, utilizing the observation device based on toxicity testing as described in claim 5, characterized in that, Includes the following steps: Step 1: Select multiple mice and divide them into three groups: a, b, and c; During the experiment, mice were administered the medication orally or by gavage. Step 2: Administer the drug to mice in group a, and increase the dosage after observing the mice recovering. Repeat this process multiple times until the dosage of mice in group a is close to the lethal dose. Mice in groups b and c are not given the drug initially. Place the mice in the main body (21) of the observation box, close the main body (21) and lock it using the locking component (22), and monitor the vital signs of the mice in the main body (21). Step 3: When the mouse leaves the floating feeding platform (31) at one end, the electronic scale (33) under the floating feeding platform (31) is zeroed. When the mouse returns to the floating feeding platform (31), the weight of the mouse on the floating feeding platform (31) is measured by the electronic scale (33). Step 4: After administering the drug to the mice, adjust the floating feeding platforms (31) at both ends to a lower position by adjusting the support component (32) so that the mice can move normally to the floating feeding platform (31) to eat. During the subsequent feeding process, adjust the support component (32) to raise the height of the floating feeding platform (31). If the mice cannot or have difficulty moving to the floating feeding platform (31) after raising the height of the floating feeding platform (31), then lower the height of the floating feeding platform (31) again. Continue until the mice are able to climb normally to the floating feeding platform (31) at a higher position. At this point, it is determined that the mice's mobility has recovered. Step 5: While performing steps 1, 2 and 3, collect and store mouse feces in real time through the collection mechanism (4), and record the time of each defecation of the mouse through the collection mechanism (4); by detecting the components of the mouse feces sample, determine whether there are toxic drug residues in the mouse feces and the amount of residues, as well as the change of the amount of toxic drug residues in the mouse feces over time. Step Six: Repeat steps Two, Three, Four, and Five until the dosage of the drug in group a mice is close to the lethal dose. At the same time, increase the dosage of the drug to both group a and group b mice. Observe the condition of the mice in groups a and b, calculate the mortality rate of the mice, and draw the corresponding experimental conclusions. Step 7: All surviving mice are assigned to group a, and mice from group c are selected to be assigned to group b. After a period of feeding and recovery, the dosage is increased again for mice in groups a and b. The condition of mice in groups a and b is observed, and the mortality rate is calculated. This operation is repeated until the mortality rate of one group of mice in groups a and b is greater than 90%, at which point the experiment ends.

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