Application of isothiazolinone compound in prevention or killing of nematodes

Abstract

The invention discloses an application of an isothiazolinone compound in prevention or killing of nematodes. The isothiazolinone compound comprises 1, 2-benzisothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, and 4, 5-dichloro-2-methoxypropyl-4-isothiazolin-3-one, and the isothiazolinone compound comprises 1, 2-benzisothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, and 4, 5-dichloro-2-methoxypropyl-4-isothiazolin-3-one. Wherein the 1, 2-benzisothiazolin-3-one and the 2-methyl-4-isothiazolin-3-one are used in a combined mode, the 1, 2-benzisothiazolin-3-one and the 4, 5-dichloro-2-methoxypropyl-4-isothiazolin-3-one are used in a combined mode, synergistic interaction is achieved, and the compound can be applied to prevention and treatment of plant disease nematodes.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to application of an isothiazolinone compound in preventing or killing nematodes. Background Art

[0002] Soybean cyst nematode (Heterodera glycines Ichinohe, hereinafter referred to as: HGI) is an important plant pathogenic nematode that mainly harms the roots of soybeans, causing the so-called "fire dragon seedling" disease. The nematode infects the tender roots of soybeans, causing symptoms such as poor root development, yellowing and early fall of leaves, delayed flowering, and clustered flower organs. In severe cases, it can cause plant death or a yield reduction of more than 50%. Soybean cyst nematode is widely distributed around the world, especially in major soybean producing countries such as the United States, Brazil, Argentina and China. The nematode is highly adaptable and can be spread through soil and crop residues, and is conducive to its growth and reproduction under high humidity and low temperature conditions. In addition, it can also enter new farmland through unloading at ports, spillage during transportation, and screenings from refineries.

[0003] At present, chemical methods are still the main means of controlling HGI. Chemical agents reported in the literature include dithiocyanate, phoxim, abamectin, clothianidin, and thiamethoxam. Although dithiocyanate has good indoor toxicity against HGI, it also has certain volatility, which is not conducive to controlling escape. Phoxim has a long residual period in the soil. Although abamectin can show high toxicity to HGI larvae and / or 2nd instar insects, its use cost is relatively high. Clothianidin and thiamethoxam have low independence against HGI and are suitable as adjuvants.

[0004] Isothiazolinone compounds are a class of organic compounds with high-efficiency and broad-spectrum bactericidal effects. Their main mechanism of action is to inhibit the growth of microorganisms by breaking the bonds between bacterial and algal proteins, and rapidly and irreversibly causing microbial cell death. There are a large number of reports on the use of isothiazolinone compounds in the prevention and treatment of fungal diseases, but there are few reports on the use of isothiazolinone compounds in plant nematode diseases, which deserves in-depth research. Summary of the invention

[0005] The object of the present invention is to provide an application of an isothiazolinone compound in preventing or killing nematodes.

[0006] Application of isothiazolinone compounds in preventing or killing nematodes. The isothiazolinone compounds include 1,2-benzisothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, and 4,5-dichloro-2-methoxypropyl-4-isothiazolin-3-one.

[0007] The isothiazolinone compound is 1,2-benzisothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, with a mass ratio of 1.0:0 to 1.0:0.2, and a preferred mass ratio is 1.2:1.0.

[0008] The isothiazolinone compound is 1,2-benzisothiazolin-3-one and 4,5-dichloro-2-methoxypropyl-4-isothiazolin-3-one, with a mass ratio of 1.0:0 to 1.0:0.1, and a preferred mass ratio is 2.1:1.0.

[0009] A pesticide for preventing or killing nematodes comprises 1,2-benzisothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, and a pesticide acceptable carrier.

[0010] A pesticide for preventing or killing nematodes comprises 1,2-benzisothiazolin-3-one, 4,5-dichloro-2-methoxypropyl-4-isothiazolin-3-one, and a pesticide acceptable carrier.

[0011] Preferably, the pesticide-acceptable carrier includes one or more of a solvent, a dispersant, an emulsifier, and a thickener.

[0012] Preferably, the solvent is one or more of water, ethanol, N-methylpyrrolidone, cyclohexanone, dimethyl carbonate, and white oil.

[0013] Preferably, the dispersant is one or more of phenylethylphenol polyoxyethylene polyoxypropylene ether, sodium methylnaphthalene sulfonate formaldehyde condensate, sodium lignin sulfonate, malonic anhydride polyether, sucrose fatty acid ester, sodium dodecyl sulfate, trisiloxane polyether, sodium tripolyphosphate, and alkylphenol polyoxyethylene ether.

[0014] Preferably, the emulsifier is one or more of fatty alcohol polyoxyethylene ether, castor oil polyoxyethylene ether, maleic rosin octylphenol polyoxyethylene ether diester carboxylate sodium, rosin polyethylene glycol citrate, sodium N-lauroyl sarcosinate and sodium di-sec-octyl maleate sulfonate.

[0015] Preferably, the thickener is one or more of xanthan gum, magnesium aluminum silicate, sodium hydroxymethyl cellulose, and polyvinyl alcohol.

[0016] The beneficial effects of the present invention are as follows: The present invention finds that 1,2-benzisothiazolin-3-one, 2-methyl-4-isothiazolin-3-one and 4,5-dichloro-2-methoxypropyl-4-isothiazolin-3-one among the isothiazolinone compounds have the effect of killing nematodes, and the combination of 1,2-benzisothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, and the combination of 1,2-benzisothiazolin-3-one and 4,5-dichloro-2-methoxypropyl-4-isothiazolin-3-one have synergistic effects and can be applied to the prevention and treatment of plant disease nematodes. DETAILED DESCRIPTION

[0017] In order to facilitate the understanding of the present invention, the present invention will be described more fully below. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0018] Example 1

[0019] A pesticide for preventing or killing nematodes is formulated according to the following components in percentage by weight:

[0020] 1,2-Benzisothiazoline-3-one 6%, 2-methyl-4-isothiazoline-3-one 5%, phenethylphenol polyoxyethylene polyoxypropylene ether 1%, sodium lignin sulfonate 1%, fatty alcohol polyoxyethylene ether 2%, xanthan gum 0.5%, water makes up to 100%.

[0021] The above components are mixed into a slurry, and sheared in a high-speed homogenizer for 8 minutes, and the average particle size of the dispersed substance is controlled to be ≤5μm to prepare.

[0022] Example 2

[0023] A pesticide for preventing or killing nematodes is formulated according to the following weight percentages:

[0024] 1,2-Benzisothiazoline-3-one 6.3%, 4,5-dichloro-2-methoxypropyl-4-isothiazoline-3-one 3%, sodium lignin sulfonate 1%, malonic anhydride polyether 1%, rosin polyethylene glycol citrate 1%, sodium N-lauroyl sarcosinate 2%, magnesium aluminum silicate 0.5%, water makes up to 100%.

[0025] The above components are mixed into a slurry, and sheared in a high-speed homogenizer for 8 minutes, and the average particle size of the dispersed substance is controlled to be ≤5μm to prepare.

[0026] Example 3

[0027] A pesticide for preventing or killing nematodes is formulated according to the following components in percentage by weight:

[0028] 1,2-Benzisothiazoline-3-one 6%, 2-methyl-4-isothiazoline-3-one 5%, sodium methylnaphthalene sulfonate formaldehyde condensate 1%, alkylphenol polyoxyethylene ether 2%, castor oil polyoxyethylene ether 1%, maleic rosin octylphenol polyoxyethylene ether diester sodium carboxylate 1%, sodium hydroxymethyl cellulose 0.5%, water to 100%.

[0029] The above components are mixed into a slurry, and sheared in a high-speed homogenizer for 8 minutes, and the average particle size of the dispersed substance is controlled to be ≤5μm to prepare.

[0030] Comparative Example 1

[0031] A pesticide for preventing or killing nematodes is formulated according to the following components in percentage by weight:

[0032] 1,2-Benzisothiazoline-3-one 11%, phenethylphenol polyoxyethylene polyoxypropylene ether 1%, sodium lignin sulfonate 1%, fatty alcohol polyoxyethylene ether 2%, xanthan gum 0.5%, water makes up to 100%.

[0033] The above components are mixed into a slurry, and sheared in a high-speed homogenizer for 8 minutes, and the average particle size of the dispersed substance is controlled to be ≤5μm to prepare.

[0034] Comparative Example 2

[0035] A pesticide for preventing or killing nematodes is formulated according to the following components in percentage by weight:

[0036] 2-Methyl-4-isothiazoline-3-one 11%, phenethylphenol polyoxyethylene polyoxypropylene ether 1%, sodium lignin sulfonate 1%, fatty alcohol polyoxyethylene ether 2%, xanthan gum 0.5%, water is added to 100%.

[0037] The above components are mixed into a slurry, and sheared in a high-speed homogenizer for 8 minutes, and the average particle size of the dispersed substance is controlled to be ≤5μm to prepare.

[0038] Comparative Example 3

[0039] A pesticide for preventing or killing nematodes is formulated according to the following components in percentage by weight:

[0040] 1,2-Benzisothiazoline-3-one 9.3%, sodium lignin sulfonate 1%, malonic anhydride polyether 1%, rosin polyethylene glycol citrate 1%, sodium N-lauroyl sarcosinate 2%, magnesium aluminum silicate 0.5%, water to 100%.

[0041] The above components are mixed into a slurry, and sheared in a high-speed homogenizer for 8 minutes, and the average particle size of the dispersed substance is controlled to be ≤5μm to prepare.

[0042] Comparative Example 4

[0043] A pesticide for preventing or killing nematodes is formulated according to the following components in percentage by weight:

[0044] 4,5-Dichloro-2-methoxypropyl-4-isothiazoline-3-one 9.3%, sodium lignin sulfonate 1%, malonic anhydride polyether 1%, rosin polyethylene glycol citrate 1%, sodium N-lauroyl sarcosinate 2%, magnesium aluminum silicate 0.5%, water to 100%.

[0045] The above components are mixed into a slurry, and sheared in a high-speed homogenizer for 8 minutes, and the average particle size of the dispersed substance is controlled to be ≤5μm to prepare.

[0046] Comparative Example 5

[0047] Avermectin 11%, phenethylphenol polyoxyethylene polyoxypropylene ether 1%, sodium lignin sulfonate 1%, fatty alcohol polyoxyethylene ether 2%, xanthan gum 0.5%, water is made up to 100%.

[0048] The above components are mixed into a slurry, and sheared in a high-speed homogenizer for 8 minutes, and the average particle size of the dispersed substance is controlled to be ≤5μm to prepare.

[0049] Comparative Example 6

[0050] A pesticide for preventing or killing nematodes is formulated according to the following components in percentage by weight:

[0051] Avermectin 9.3%, sodium lignin sulfonate 1%, malonic anhydride polyether 1%, rosin polyethylene glycol citrate 1%, sodium N-lauroyl sarcosinate 2%, magnesium aluminum silicate 0.5%, water is added to 100%.

[0052] The above components are mixed into a slurry, and sheared in a high-speed homogenizer for 8 minutes, and the average particle size of the dispersed substance is controlled to be ≤5μm to prepare.

[0053] Experimental Example 1:

[0054] Select soybean planting fields with severe soybean cyst nematode infection and apply pesticide to soybean seeds (dosage: 60 g / kg) before sowing. Each treatment was repeated three times, with a 30 m2 per plot. 2 Each plot was arranged in random blocks, the pesticides were the pesticides prepared in Example 1, Example 3, and Comparative Examples 1-2, and the control was not treated with the pesticides.

[0055] Samples were taken at five points in each plot before soybean sowing and after soybean harvest. 10 cm of soil was dug with a soil sampler, air-dried and mixed, 500 g of soil was weighed, and cysts in the soil were separated by sieve flotation method. The number of full cysts in the soil at each sampling point was counted under a stereo microscope, and the cyst reduction rate was calculated according to the following formula:

[0056] Cyst reduction rate (%) = (number of cysts before sowing - number of cysts after sowing) / number of cysts before sowing × 100.

[0057] SPSS 24.0 software was used for statistical analysis, and the quantitative data were analyzed by (mean ± standard deviation), the Kolmogorov-Smirnov test was used for data normality test, and the t-test was used to compare the mean differences between the two groups for data that met the normal distribution, with P < 0.05 as the difference being statistically significant. The results are shown in Table 1:

[0058] Table 1

[0059]

[0060]

[0061] Note: * represents P < 0.05 compared with the group in Example 1.

[0062] Experimental Example 2:

[0063] Wheat fields with severe wheat cyst nematode infection were selected and the seeds were treated with pesticides (dosage was 60 g / kg) before sowing. Each treatment was repeated three times and the area was 30 m2 per plot. 2 Each plot was arranged in random blocks, the pesticides were the pesticides prepared in Example 2 and Comparative Examples 3-4, and the control was not treated with the pesticides.

[0064] Samples were taken at five points in each plot before wheat sowing and after wheat harvest. 10 cm of soil was dug with a soil sampler, air-dried and mixed, 500 g of soil was weighed, and cysts in the soil were separated by sieve flotation method. The number of full cysts in the soil at each sampling point was counted under a stereo microscope, and the cyst reduction rate was calculated according to the following formula:

[0065] Cyst reduction rate (%) = (number of cysts before sowing - number of cysts after sowing) / number of cysts before sowing × 100.

[0066] SPSS 24.0 software was used for statistical analysis, and the quantitative data were analyzed by (mean ± standard deviation) was expressed, and the Kolmogorov-Smirnov test was used for data normality test. For data that met the normal distribution, the mean difference between the two groups was compared using the t test, and the difference was considered statistically significant when P < 0.05. The measurement results are shown in Table 2:

[0067] Table 2

[0068]

[0069] Note: * represents P < 0.05 compared with the group in Example 2.

[0070] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. Use of an isothiazolinone compound in preventing or killing nematodes, characterized in that: The isothiazolinone compounds include 1,2-benzisothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, and 4,5-dichloro-2-methoxypropyl-4-isothiazolin-3-one.

2. The use of the isothiazolinone compound in preventing or killing nematodes according to claim 1, characterized in that: The isothiazolinone compound is 1,2-benzisothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, and the mass ratio is 1.0:0 to 1.0:0.

2.

3. The use of the isothiazolinone compound in preventing or killing nematodes according to claim 1, characterized in that: The isothiazolinone compound is 1,2-benzisothiazolin-3-one and 4,5-dichloro-2-methoxypropyl-4-isothiazolin-3-one, and the mass ratio is 1.0:0 to 1.0:0.

1.

4. A pesticide for preventing or killing nematodes, characterized in that: The invention comprises 1,2-benzisothiazolin-3-one, 2-methyl-4-isothiazolin-3-one and a pesticide-acceptable carrier.

5. A pesticide for preventing or killing nematodes, characterized in that: The invention comprises 1,2-benzisothiazolin-3-one, 4,5-dichloro-2-methoxypropyl-4-isothiazolin-3-one and a pesticide-acceptable carrier.

6. The pesticide for preventing or killing nematodes according to claim 4 or 5, characterized in that: The pesticide-acceptable carrier includes one or more of a solvent, a dispersant, an emulsifier, and a thickener.

7. The pesticide for preventing or killing nematodes according to claim 4 or 5, characterized in that: The solvent is one or more of water, ethanol, N-methylpyrrolidone, cyclohexanone, dimethyl carbonate and white oil.

8. The pesticide for preventing or killing nematodes according to claim 4 or 5, characterized in that: The dispersant is one or more of phenylethylphenol polyoxyethylene polyoxypropylene ether, sodium methylnaphthalene sulfonate formaldehyde condensate, sodium lignin sulfonate, malonic anhydride polyether, sucrose fatty acid ester, sodium dodecyl sulfonate, trisiloxane polyether, sodium tripolyphosphate, and alkylphenol polyoxyethylene ether.

9. The pesticide for preventing or killing nematodes according to claim 4 or 5, characterized in that: The emulsifier is one or more of fatty alcohol polyoxyethylene ether, castor oil polyoxyethylene ether, maleic rosin octylphenol polyoxyethylene ether diester carboxylate sodium, rosin polyethylene glycol citrate, sodium N-lauroyl sarcosinate and sodium di-sec-octyl maleate sulfonate.

10. The pesticide for preventing or killing nematodes according to claim 4 or 5, characterized in that: The thickener is one or more of xanthan gum, magnesium aluminum silicate, sodium hydroxymethyl cellulose and polyvinyl alcohol.

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