Elastin-like polypeptide V20K40L, mRNA vaccine and preparation method and application thereof

Abstract

The invention discloses elastin-like polypeptide V20K40L, an mRNA vaccine, a preparation method of the elastin-like polypeptide V20K40L and application of the elastin-like polypeptide V20K40L and the mRNA vaccine in preparation of drugs for preventing, relieving or treating tumors The elastin-like polypeptide carrier V20K40L provided by the invention has relatively good biocompatibility, and has no adverse reaction in vivo. The elastin-like polypeptide V20K40L provided by the invention has an amphiphilic structure, can efficiently load Melan-AMRNA through hydrophilic and hydrophobic self-assembly and electrostatic adsorption, and is in a tightening state when the temperature is higher than Tt, so that uncontrollable leakage of mRNA can be prevented. Moreover, the V20K40L contains lysosome escape peptide, so that the transfection efficiency of mRNA can be further improved by promoting lysosome escape. The elastin-like polypeptide V20K40L disclosed by the invention can be used for efficiently and safely delivering Melan-AmRNA, so that the melanoma can be prevented and treated.

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